Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase A regulatory subunit I alpha monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of paraffin-embedded carcinoma of bladder tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50).

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase A regulatory subunit I alpha.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded colon tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded endometrium tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Ovary tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded prostate tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI6C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector
• Specificity: This antibody is specific for Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

• cAMP-dependent protein kinase regulatory subunit RIalpha
• cAMP-dependent protein kinase type I-alpha regulatory chain
• cAMP-dependent protein kinase type I-alpha regulatory subunit
• CAR
• CNC
• CNC1
• MGC17251
• PKA R1 alpha
• PKA1
• PKR1
• PPNAD1
• PRKAR1
• PRKAR1A
• Protein Kinase A regulatory subunit I alpha
• protein kinase A type 1a regulatory subunit
• protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1)
• Tissue-specific extinguisher 1
• TSE1
• TSE1DKFZp779L0468

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein Kinase A regulatory subunit I alpha Antibody (NBP1-47935)(1)

Publication using NBP1-47935

• Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016-05-31 [PMID: 27245613] (WB, Human)

Review for Protein Kinase A regulatory subunit I alpha Antibody (NBP1-47935) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Daniel Popoola WB Rat 02/13/2017

Summary

• Application: Western Blot
• Sample Tested: Dorsal Hippocampus Brain Tissue
• Species: Rat

Comments

• Comments: Diluted 1:2000

Bioinformatics

• Gene Symbol: PRKAR1A
• Entrez:
  • 5573(Human)