LRH-1/NR5A2 Antibody

Images

 
Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat ...read more
Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, ChHa, Eq, Gp, Ma-Op, Pm, Rb, RM, ZeSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LRH-1/NR5A2 Antibody Summary

Immunogen
A portion of amino acids 140-190 from human NR5A2 was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR5A2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5ug/ml~
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Knockdown Validated
Application Notes
Knockdown validation (PMID: 31978970).
Publications
Read Publication using
NBP2-27196 in the following applications:

  • KD
    1 publication
  • WB
    1 publication

Reactivity Notes

Orangutan (100%), Chimpanzee (100%), New World Monkey (100%), Panda (100%), Great Ape (100%), Mole Rat (100%), White cheeked gibbon (100%), Platypus (100%), Zebra finch (100%), Catfish (100%), Frog (100%), Carolina anole (100%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for LRH-1/NR5A2 Antibody

  • Alpha-1-fetoprotein transcription factor
  • B1-binding factor
  • b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
  • B1F
  • B1F2
  • fetoprotein-alpha 1 (AFP) transcription factor
  • FTFLRH1
  • FTZ-F1 beta
  • FTZ-F1
  • FTZ-F1beta
  • HB1F
  • hB1F-2
  • hB1FCYP7A promoter-binding factor
  • Hepatocytic transcription factor
  • Liver receptor homolog 1
  • liver receptor homolog-1
  • LRH1
  • LRH-1
  • LRH-1CPF
  • NR5A2
  • nuclear receptor NR5A2
  • nuclear receptor subfamily 5 group A member 2
  • nuclear receptor subfamily 5, group A, member 2

Background

NR5A2 is a member of the so-called NR5A family of nuclear receptors. NR5A2, also call LRH1 or Liver Receptor Homolog 1, appears to play an important role in regulating pancreatic and embryonal stem cell differentiation maintaining a pluripotential or undifferentiated state. It also plays a role in controlling cholesterol balance (1, 2). NR5A2 may control pluripotentiality through its regulation of Oct4 and Nanog, both are commonly held as requisite factors for maintenance of stem cell undifferentiated states (3). Additionally, NR5A2 has a role in colon, pancreatic and breast cancer, accompanied by overexpression in the latter in ductal carcinomas (4, 5). Antibody to NR5A2 provides a tool to link expression and function with undifferentiated states in embryonic stem cells or in cancers of the breast, intestine, and pancreas, and for further characterization of NR5A2 as a potential biomarker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRH-1/NR5A2 Antibody (NBP2-27196)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 2 applications: KD, WB.


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Bioinformatics Tool for LRH-1/NR5A2 Antibody (NBP2-27196)

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Bioinformatics

Gene Symbol NR5A2