Arrestin 3 Antibody

Immunohistochemistry-Paraffin: Arrestin 3 Antibody [NBP1-37003] - Staining (2.5ug/ml) of paraffin embedded Human Retina. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Immunohistochemistry-Paraffin: Arrestin 3 Antibody [NBP1-37003] - Negative Control showing staining of paraffin embedded Human Retina, with no primary antibody.

• Reactivity: Hu, Pm
• Applications: ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Arrestin 3 Antibody Summary

• Immunogen: Arrestin 3 Antibody is made to a peptide with sequence C-RKGEEESQKAVE corresponding to C-Terminus according to NP_004303.2.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:64000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35024589).Use in ICC/IF reported in scientific literature (PMID: 30567931)..

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Arrestin 3 Antibody

• arrestin 3, retinal (X-arrestin)
• arrestin 4
• arrestin-C
• ARRXcArr
• CAR
• C-arrestin
• Cone arrestin
• Retinal cone arrestin-3
• X-arrestin

Background

Arrestin-C, also known as retinal cone arrestin-3, is a protein involved in the regulation of retinal cone-specific photo signal transduction. Arrestin 3 antibodies are useful for phototransduciton studies and general vision research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arrestin 3 Antibody (NBP1-37003)(6)

Publications using NBP1-37003

• Saha A, Capowski E, Fernandez Zepeda MA et al. Cone photoreceptors in human stem cell-derived retinal organoids demonstrate intrinsic light responses that mimic those of primate fovea Cell stem cell 2022-01-25 [PMID: 35104442] (ICC/IF, Human)
• Yamasaki S, Tu H, Matsuyama T et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation iScience 2022-01-01 [PMID: 35024589] (IHC-Fr, Monkey)
• Cowan CS, Renner M, De Gennaro M et al. Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution Cell 2020-09-17 [PMID: 32946783] (Human)
• Sridhar A, Hoshino A, Finkbeiner CR et al. Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures Cell Rep 2020-02-04 [PMID: 32023475] (Human)
• Capowski EE, Samimi K, Mayerl SJ et al. Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines Development 2018-12-19 [PMID: 30567931] (ICC/IF, Human)
• Krishnamurthy H, Galet C, Ascoli M. The association of arrestin-3 with the follitropin receptor depends on receptor activation and phosphorylation. Mol Cell Endocrinol 2003-06-30 [PMID: 12850288]

Bioinformatics

• Gene Symbol: ARR3
• Entrez:
  • 407(Human)
• Uniprot:
  • NP_004303.1(Human)