CYP7A1 Antibody - Azide and BSA Free

Western Blot: CYP7A1 Antibody [NBP3-04836] - Analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836] - Immunofluorescence analysis of HepG2 cells using CYP7A1 Rabbit pAb (A10615) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CYP7A1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CYP7A1 Antibody - Azide and BSA Free (NBP3-04836) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2). YPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP7A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for CYP7A1 Antibody - Azide and BSA Free

• Cholesterol 7-alpha-hydroxylase
• cholesterol 7-alpha-monooxygenase
• CP7A
• CYP7
• CYP7A1
• CYP7MGC126826
• CYPVII
• Cytochrome P450 7A1
• cytochrome P450, family 7, subfamily A, polypeptide 1
• cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase)
• cytochrome P450, subfamily VIIA polypeptide 1
• EC 1.14.13
• EC 1.14.13.17
• MGC138389
• polypeptide 1

Background

The CYP7A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP7A1