Human FGF-19 Quantikine ELISA Kit Summary
The Quantikine Human FGF-19 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-19 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-19 showed linear curves that were parallel to the standard curves obt...ained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-19.
Natural and recombinant human FGF-19
Solid Phase Sandwich ELISA
No significant interference observed with available related molecules.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human FGF-19 Quantikine ELISA Kit
- fibroblast growth factor 19
The Fibroblast Growth Factor (FGF) family consists of at least 22 highly conserved proteins and
are found in many species ranging from C. elegans and Drosophila to humans (1). FGFs are
heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a
common tertiary structure. In general, FGFs are expressed during embryonic development and
in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to
regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation,
metabolic regulation, cell migration, neurotrophic effects, and tissue repair (2). The activities of
the FGF family are mediated by four receptors, FGF R1 through FGF R4 (2). The receptors are
transmembrane proteins with a tyrosine kinase domain and are thought to act as classical
receptor tyrosine kinases. FGF R5/FGF RL1 has also been described, but lacks the kinase
domain and signaling capability (3).
Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a
229 aa secreted mature protein with no potential N-linked glycosylation sites (4, 5). It shares
approximately 50% aa sequence identity with mouse and rat FGF-15 and 62% aa sequence
identity with chicken FGF-19 (4-6). Unlike most FGFs, which bind to and activate more than one
FGF receptor, FGF-19 appears to bind only FGF R4 (5). Receptor binding is capable of activating
MAP Kinase and inducing the Prolactin promoter (7). FGF-19 has two putative disulfide bonds,
one of which is conserved in the FGF family (8). Uniquely, it also has an extended loop structure
that may affect heparan sulfate binding (8).
The functional roles of FGF-19 continue to be elucidated and include developmental,
metabolic, and tumorigenic activities. During chick embryogenesis, FGF-19 has been shown to
act synergistically with Wnt-8c to initiate inner ear development (6). However, the mouse
FGF-19 ortholog, FGF-15, is not required for this activity (9). FGF-19 is also expressed in the
embryonic chicken eye where it may play a role in lens development (10). Questions regarding
the functions of human FGF-19 have been addressed using ectopic expression. Transgenic
over-expression of human FGF-19 in mice results in tumor formation, increased energy
expenditure, decreased adiposity, and a resistance to weight gain in response to a high fat diet
(11, 12). Similar affects have been observed in mice treated intravenously with recombinant
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
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