Human FGF-19 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human FGF-19 Quantikine ELISA Kit Summary

Background
The Quantikine Human FGF-19 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-19 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-19 showed linear curves that were parallel to the standard curves obt...ained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-19.
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Specificity
Natural and recombinant human FGF-19
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
FGF19

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DF1900.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FGF-19 Quantikine ELISA Kit

  • FGF19
  • FGF-19
  • fibroblast growth factor 19

Background

The Fibroblast Growth Factor (FGF) family consists of at least 22 highly conserved proteins and are found in many species ranging from C. elegans and Drosophila to humans (1). FGFs are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. In general, FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, metabolic regulation, cell migration, neurotrophic effects, and tissue repair (2). The activities of the FGF family are mediated by four receptors, FGF R1 through FGF R4 (2). The receptors are transmembrane proteins with a tyrosine kinase domain and are thought to act as classical receptor tyrosine kinases. FGF R5/FGF RL1 has also been described, but lacks the kinase domain and signaling capability (3). 
Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (4, 5). It shares approximately 50% aa sequence identity with mouse and rat FGF-15 and 62% aa sequence identity with chicken FGF-19 (4-6). Unlike most FGFs, which bind to and activate more than one FGF receptor, FGF-19 appears to bind only FGF R4 (5). Receptor binding is capable of activating MAP Kinase and inducing the Prolactin promoter (7). FGF-19 has two putative disulfide bonds, one of which is conserved in the FGF family (8). Uniquely, it also has an extended loop structure that may affect heparan sulfate binding (8). 
The functional roles of FGF-19 continue to be elucidated and include developmental, metabolic, and tumorigenic activities. During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (6). However, the mouse FGF-19 ortholog, FGF-15, is not required for this activity (9). FGF-19 is also expressed in the embryonic chicken eye where it may play a role in lens development (10). Questions regarding the functions of human FGF-19 have been addressed using ectopic expression. Transgenic over-expression of human FGF-19 in mice results in tumor formation, increased energy expenditure, decreased adiposity, and a resistance to weight gain in response to a high fat diet (11, 12). Similar affects have been observed in mice treated intravenously with recombinant FGF-19 (13).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for FGF-19 (DF1900)(21)

We have publications tested in 1 confirmed species: Human.


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Human
(21)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using DF1900 Applications Species
WS Liu, MJ Tang, TL Xu, JB Su, XQ Wang, F Xu, DM Zhang, Q Zhu, J Cao, H Wang Association of serum fibroblast growth factor 19 levels with arteriosclerosis parameters assessed by arterial stiffness and atherogenic index of plasma in patients with type 2 diabetes Diabetol Metab Syndr, 2020;12(0):44. 2020 [PMID: 32477430] (Human) Human
AE O'Mara, JW Johnson, JD Linderman, RJ Brychta, S McGehee, LA Fletcher, YA Fink, D Kapuria, TM Cassimatis, N Kelsey, C Cero, Z Abdul-Sate, F Piccinini, AS Baskin, BP Leitner, H Cai, CM Millo, W Dieckmann, M Walter, NB Javitt, Y Rotman, PJ Walter, M Ader, RN Bergman, P Herscovitc, KY Chen, AM Cypess Chronic mirabegron treatment increases human brown fat, HDL cholesterol, and insulin sensitivity J. Clin. Invest., 2020;0(0):. 2020 [PMID: 31961826] (Human) Human
MJ Tang, JB Su, TL Xu, XQ Wang, DM Zhang, XH Wang Serum fibroblast growth factor 19 and endogenous islet beta cell function in type 2 diabetic patients Diabetol Metab Syndr, 2019;11(0):79. 2019 [PMID: 31572498] (Human) Human
RN Appleby, I Moghul, S Khan, M Yee, P Manousou, TD Neal, JRF Walters Non-alcoholic fatty liver disease is associated with dysregulated bile acid synthesis and diarrhea: A prospective observational study PLoS ONE, 2019;14(1):e0211348. 2019 [PMID: 30682184] (Human) Human
L Gao, L Lang, X Zhao, C Shay, AY Shull, Y Teng FGF19 amplification reveals an oncogenic dependency upon autocrine FGF19/FGFR4 signaling in head and neck squamous cell carcinoma Oncogene, 2018;0(0):. 2018 [PMID: 30518874] (Human) Human
T Morville, RE Sahl, SA Trammell, JS Svenningse, MP Gillum, JW Helge, C Clemmensen Divergent effects of resistance and endurance exercise on plasma bile acids, FGF19, and FGF21 in humans JCI Insight, 2018;3(15):. 2018 [PMID: 30089729] (Human) Human
G Cui, RC Martin, H Jin, X Liu, H Pandit, H Zhao, L Cai, P Zhang, W Li, Y Li Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver J. Exp. Clin. Cancer Res., 2018;37(1):136. 2018 [PMID: 29973237] (Human) Human
D Friedrich, HU Marschall, F Lammert Response of fibroblast growth factor 19 and bile acid synthesis after a body weight-adjusted oral fat tolerance test in overweight and obese NAFLD patients: a non-randomized controlled pilot trial BMC Gastroenterol, 2018;18(1):76. 2018 [PMID: 29866129] (Human) Human
S Al-Dury, A Wahlström, S Wahlin, J Langedijk, RO Elferink, M Ståhlman, HU Marschall Pilot study with IBAT inhibitor A4250 for the treatment of cholestatic pruritus in primary biliary cholangitis Sci Rep, 2018;8(1):6658. 2018 [PMID: 29704003] (Human) Human
JM Lee, JR Ong, L Vergnes, TQ de Aguiar, J Nolan, RM Cantor, JRF Walters, K Reue Diet1, bile acid diarrhea, and FGF15/19: mouse model and human genetic variants J. Lipid Res., 2018;0(0):. 2018 [PMID: 29295820] (Human) Human
Show All 21 Publications.

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FAQs for FGF-19 (DF1900). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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Bioinformatics

Gene Symbol FGF19