Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SPG7 Antibody (1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Use in Immunohistochemistry reported in scientific literature (PMID 28131770).
88.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP2-01860 in the following applications:
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA, 50% Glycerol
0.02% Sodium Azide
Alternate Names for SPG7 Antibody (OTI1C1)
cell matrix adhesion regulator
PGNcell adhesion regulator
spastic paraplegia 7 (pure and complicated autosomal recessive)
Spastic paraplegia 7 protein
This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Mouse liver lysate processed and loaded in 12% TGX gel in reduced and denaturalization conditions. Milk 5% blocking in PBST (0.1% Tween) 1h. Primary antibody 1:1000 in Milk 5% in PBST (0.1% Tween) O/N 4ºC. Secondary Donkey anti-mouse Alexa fluor 555 1:5000 1h RT. Band observed around 75 kDa.
Product General Protocols
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.