SPG7 Antibody (OTI1C1)

Images

 
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Biological Strategies: Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. ...read more
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

SPG7 Antibody (OTI1C1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPG7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:500 - 2000
Application Notes
Use in IHC reported in scientific literature (PMID 28131770).
Theoretical MW
88.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-01860 in the following applications:

Publications
Read Publications using
NBP2-01860 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPG7 Antibody (OTI1C1)

  • CARMGC126331
  • cell matrix adhesion regulator
  • CMARMGC126332
  • EC 3.4.24
  • EC 3.4.24.-
  • FLJ37308
  • paraplegin
  • PGNcell adhesion regulator
  • spastic paraplegia 7 (pure and complicated autosomal recessive)
  • Spastic paraplegia 7 protein
  • SPG5C

Background

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPG7 Antibody (NBP2-01860)(3)

Review for SPG7 Antibody (NBP2-01860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-01860:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot SPG7 NBP2-01860
Enlarge
4
reviewed by:
Marina Villar
WB Mouse 11/22/2020
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Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse
LotF001

Comments

CommentsMouse liver lysate processed and loaded in 12% TGX gel in reduced and denaturalization conditions. Milk 5% blocking in PBST (0.1% Tween) 1h. Primary antibody 1:1000 in Milk 5% in PBST (0.1% Tween) O/N 4ºC. Secondary Donkey anti-mouse Alexa fluor 555 1:5000 1h RT. Band observed around 75 kDa.

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Recent Reviews

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4
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1
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Marina Villar
11/22/2020
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SPG7
Entrez