SPG7 Antibody (OTI1C1)

Images

 
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SPG7 Antibody (1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SPG7 Antibody (OTI1C1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPG7
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28131770).
Theoretical MW
88.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-01860 in the following applications:

Publications
Read Publications using
NBP2-01860 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for SPG7 Antibody (OTI1C1)

  • CARMGC126331
  • cell matrix adhesion regulator
  • CMARMGC126332
  • EC 3.4.24
  • EC 3.4.24.-
  • FLJ37308
  • paraplegin
  • PGNcell adhesion regulator
  • spastic paraplegia 7 (pure and complicated autosomal recessive)
  • Spastic paraplegia 7 protein
  • SPG5C

Background

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPG7 Antibody (NBP2-01860)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Human
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Mouse
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Review for SPG7 Antibody (NBP2-01860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-01860:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot SPG7 NBP2-01860
Enlarge
4
reviewed by:
Anonymous
WB Mouse 11/22/2020
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse
LotF001

Comments

CommentsMouse liver lysate processed and loaded in 12% TGX gel in reduced and denaturalization conditions. Milk 5% blocking in PBST (0.1% Tween) 1h. Primary antibody 1:1000 in Milk 5% in PBST (0.1% Tween) O/N 4ºC. Secondary Donkey anti-mouse Alexa fluor 555 1:5000 1h RT. Band observed around 75 kDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SPG7 Antibody (NBP2-01860) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74327AF350
Alexa Fluor 405 NBP2-74327AF405
Alexa Fluor 488 NBP2-74327AF488
Alexa Fluor 532 NBP2-74327AF532
Alexa Fluor 594 NBP2-74327AF594
Alexa Fluor 647 NBP2-74327AF647
Alexa Fluor 700 NBP2-74327AF700
Alexa Fluor 750 NBP2-74327AF750
Allophycocyanin NBP2-74327APC
Allophycocyanin/Cy7 NBP2-74327APCCY7
Biotin NBP2-74327B
DyLight 350 NBP2-74327UV
DyLight 405 NBP2-74327V
DyLight 488 NBP2-74327G
DyLight 550 NBP2-74327R
DyLight 594 NBP2-74327DL594
DyLight 650 NBP2-74327C
DyLight 680 NBP2-74327FR
DyLight 755 NBP2-74327IR
FITC NBP2-74327F
HRP NBP2-74327H
Janelia Fluor 549 NBP2-74327JF549
Janelia Fluor 646 NBP2-74327JF646
PE NBP2-74327PE
PE/Atto594 NBP2-74327PEATT594
PE/Cy5.5 NBP2-74327PECY55
PE/Cy7 NBP2-74327PECY7
PerCP NBP2-74327PCP

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
11/22/2020
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SPG7
Entrez