TGR5/GPBAR1 Antibody

Images

 
Western Blot: TGR5/GPBAR1 Antibody [NBP2-23669] - Analysis of spleen lysate. (1) Pre-immune sera. TGR5/GPBAR1 antibody tested on (2) Human 1:1000, (3) Mouse 1:5000, and (4) Rat 1:5000 spleen lysates.
Immunocytochemistry/ Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: TGR5/GPBAR1 Antibody [NBP2-23669] - Tissue section of mouse colon using TGR5/GPBAR1 antibody at 1:200 dilution. The signal was detected using HRP-labelled secondary antibody and DAB ...read more
Immunocytochemistry/ Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Genetic Strategies: Knockdown Validated: TGR5/GPBAR1 Antibody [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5/GPBAR1 and by CAR receptors. TGR5/GPBAR1, CAR, VDR, and PXR bile acid ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

TGR5/GPBAR1 Antibody Summary

Immunogen
Synthetic peptide made to an internal region of the human TGR5/GPBAR1 protein (between amino acids 250-300) [UniProt Q8TDU6]
Predicted Species
Primate (98%), Rabbit (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPBAR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
Publications
Read Publications using
NBP2-23669 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TGR5/GPBAR1 Antibody

  • BG37
  • BG37hBG37
  • G protein-coupled bile acid receptor 1
  • GPBA
  • GPBAR1
  • GPCR19
  • GPR131
  • G-protein coupled bile acid receptor 1
  • G-protein coupled bile acid receptor BG37
  • G-protein coupled receptor GPCR19
  • M-BAR
  • M-BARhGPCR19
  • membrane bile acid receptor
  • Membrane-type receptor for bile acids
  • MGC40597
  • TGR5
  • TGR5GPCR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGR5/GPBAR1 Antibody (NBP2-23669)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP2-23669 Applications Species
Miko E, Vida A, KovAcs T et al. Lithocholic acid, a bacterial metabolite reduces breast cancer cell proliferation and aggressiveness. Biochim. Biophys. Acta Apr 12 2018 [PMID: 29655782] (Mouse) Mouse
Sun S, Guo H, Liang N et al. GPBAR1 Promotes Proliferation and Is Related to Poor Prognosis of High-Grade Glioma via Inducing MAFB Expression Research Square May 7 2021
Beck L De invloed van zwangerschapscholestase en de behandeling met Ursochol op de verhouding van macrofagen subset M1 en M2 en de TGR5 receptor Thesis 2017 Jun 01 (IHC, Human) IHC Human
Trah J, Arand J, Oh J et al. Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option Sci Rep Nov 23 2020 [PMID: 33230229] (WB) WB

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGR5/GPBAR1 Antibody (NBP2-23669) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-23669AF350
Alexa Fluor 405 NBP2-23669AF405
Alexa Fluor 488 NBP2-23669AF488
Alexa Fluor 532 NBP2-23669AF532
Alexa Fluor 594 NBP2-23669AF594
Alexa Fluor 647 NBP2-23669AF647
Alexa Fluor 700 NBP2-23669AF700
Alexa Fluor 750 NBP2-23669AF750
Biotin NBP2-23669B
DyLight 350 NBP2-23669UV
DyLight 405 NBP2-23669V
DyLight 488 NBP2-23669G
DyLight 550 NBP2-23669R
DyLight 594 NBP2-23669DL594
DyLight 650 NBP2-23669C
DyLight 680 NBP2-23669FR
DyLight 755 NBP2-23669IR
FITC NBP2-23669F
HRP NBP2-23669H
Janelia Fluor 549 NBP2-23669JF549
Janelia Fluor 646 NBP2-23669JF646

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Bioinformatics Tool for TGR5/GPBAR1 Antibody (NBP2-23669)

Discover related pathways, diseases and genes to TGR5/GPBAR1 Antibody (NBP2-23669). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TGR5/GPBAR1.

The TGR5 Receptor and metabolic disease
The bile acid-responsive G-protein-coupled receptor TGR5 is widely distributed across the human body - including the endocrine glands, adipocyte cells, muscles, immune organs, spinal cord, and the enteric nervous system. G protein coupled receptor...  Read full blog post.

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Bioinformatics

Gene Symbol GPBAR1
Uniprot