FXR/NR1H4 Antibody [HRP]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

FXR/NR1H4 Antibody [HRP] Summary

• Immunogen: A synthetic peptide made to the C-terminus of the human FXR protein. [UniProt# Q96RI1]
• Localization: Cytoplasmic, nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1H4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This FXR antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis, where a band is seen at ~66 kDa. In ICC/IF nuclear and cytoplasmic staining was observed.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29491146).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FXR/NR1H4 Antibody [HRP]

• BAR
• bile acid receptor
• farnesoid X receptor
• Farnesoid X-activated receptor
• Farnesol receptor HRR-1
• FXR
• FXRBAR
• HRR-1
• HRR1MGC163445
• NR1H4
• Nuclear receptor subfamily 1 group H member 4
• nuclear receptor subfamily 1, group H, member 4
• Retinoid X receptor-interacting protein 14
• RIP14
• RIP14RXR-interacting protein 14

Background

The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR. FXR and LXR fall into a category of proteins termed "orphan receptors" because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR1H4