MRP2 Antibody (M2III-6)

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: M2III-6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

MRP2 Antibody (M2III-6) Summary

• Description: Novus Biologicals Mouse MRP2 Antibody (M2III-6) (NBP1-42349) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-MRP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: 202-amino acid sequence at C-terminal end of MRP2
• Specificity: Reacts with an internal epitope of MRP2. Does not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Western Blot 1:20-1:50
• Application Notes: M2III-6 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-6 (use 1:20) on acetone fixed frozen sections

Reactivity Notes

Use in Rat reported in scientific literature (PMID: 32697600).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for MRP2 Antibody (M2III-6)

• ABC30
• ABCC2
• ATP-binding cassette, sub-family C (CFTR/MRP), member 2
• Canalicular multidrug resistance protein
• canalicular multispecific organic anion transporter 1
• CMOAT
• CMOAT1
• CMOATABC30
• cMRP
• DJS
• KIAA1010
• MRP2
• MRP2ATP-binding cassette sub-family C member 2
• Multidrug resistance-associated protein 2

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRP2 Antibody (NBP1-42349)(1)

Publication using NBP1-42349

• Choi JH, Loarca L, De Hoyos-Vega JM et al. Microfluidic confinement enhances phenotype and function of hepatocyte spheroids Am. J. Physiol., Cell Physiol. 2020-07-22 [PMID: 32697600] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: ABCC2
• Entrez:
  • 1244(Human)
• Uniprot:
  • Q92887(Human)