CXADR Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue and mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by ...read more
Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. Specific bands were detected for CXADR at approximately 60 and 53 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse CXADR ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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CXADR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CXADR
Leu20-Gly237
Accession # P97792
Specificity
Detects mouse CXADR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross‑reactivity with recombinant human CXADR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXADR
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 12.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2654 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXADR Antibody [Unconjugated]

  • CAR10Coxsackievirus B-adenovirus receptor
  • CAR4/6
  • coxsackie virus and adenovirus receptor
  • coxsackie virus B receptor
  • coxsackievirus and adenovirus receptor
  • CVB3 binding protein
  • CVB3 BP
  • CVB3-binding protein
  • CXADR
  • HCAR
  • HCVADR

Background

CXADR (Coxsackie and Adenovirus Receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) mouse CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). A PDZ binding motif at the C‑terminus interacts with several cytoplasmic junctional proteins (1). The ECD of mouse CXADR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows the same expression pattern, but may show different subcellular localization (4, 8). Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection (9).

  1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
  2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
  3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
  4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
  5. Hotta, Y. et al. (2003) Dev. Brain Res. 143:1.
  6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
  7. Chen, J. et al. (2006) Circ. Res. 98:923.
  8. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
  9. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXADR Antibody (AF2654)(3)

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Secondary Antibodies

 

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Biotin BAF2654

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Bioinformatics

Gene Symbol CXADR
Uniprot