Calcium-sensing R/CaSR Antibody (5C10, ADD)

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CaSR in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue.

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of calcium sensing receptor in HEK7-2 cell extract.

Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody. Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at 4C in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8 um cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4C, immersing in sodium citrate buffer for 10 minutes at 100C before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with NB120-19347 (diluted 1:100 in blocking buffer) overnight at 4C followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT. Sections were also stained with DAPI nuclear stain (blue). NB120-19347 positive cells (green) were found at the base of the antral glands in the mouse stomach. Data courtesy of the Innovators Program

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human brain tissue.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 5C10, ADD
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Calcium-sensing R/CaSR Antibody (5C10, ADD) Summary

• Immunogen: Partial synthetic peptide corresponding to residues 214 - 235 of human calcium sensing receptor R / CaSR [UniProt P41180]
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: IHC-P usage was reported in scientific literature. In Western blot this antibody detects bands at approximately 130 and 150 kDa. We have conflicting data on the use of this antibody in Flow.
• Control:

  Calcium-sensing R/CaSR Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calcium-sensing R/CaSR Antibody (5C10, ADD)

• calcium-sensing receptor
• CAR
• CaSR
• EIG8
• extracellular calcium-sensing receptor
• FHH
• FIH
• GPRC2A
• GPRC2AHHC
• HHC1
• hypocalciuric hypercalcemia 1
• NSHPT
• parathyroid Ca(2+)-sensing receptor 1
• Parathyroid cell calcium-sensing receptor
• PCaR1
• PCAR1MGC138441

Background

The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcium-sensing R/CaSR Antibody (NB120-19347)(20)

Publications using NB120-19347

• Nery F, Siranosian J, Rosales I et al. Impaired kidney structure and function in spinal muscular atrophy Neurol Genet Oct 1 2019 [PMID: 31517062] (IHC-P, Human)
• Bachinger D, Egli H, Goosmann MM et al. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear Cell Tissue Res. Jul 23 2019 [PMID: 31338584] (IHC-P, Mouse)
• Luchsinger LL, Strikoudis A, Danzl NM et al. Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro Cell Stem Cell 2019 May 31 [PMID: 31178255] (Flow, Mouse, Human)
• Issa H, Henaut L, Abdallah JB et al. Activation of the calcium-sensing receptor in human valvular interstitial cells promotes calcification J. Mol. Cell. Cardiol. Feb 12 2019 [PMID: 30769016] (WB, IHC-Fr, Human)
• Frees S, Breuksch I, Haber T et al. Calcium-sensing receptor (CaSR) promotes development of bone metastasis in renal cell carcinoma. Oncotarget. [PMID: 29644008] (Flow, WB, Human)
• Boudot C, Henaut L, Thiem U et al. Overexpression of a functional calcium-sensing receptor dramatically increases osteolytic potential of MDA-MB-231 cells in a mouse model of bone metastasis through epiregulin-mediated osteoprotegerin downregulation Oncotarget 2017 Aug 22 [PMID: 28915604] (WB, Mouse)
• Dai XQ, Perez PL, Soria G et al. External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells. Exp. Cell Res. Nov 9 2016 [PMID: 27836810] (Porcine)
• Koh J, Hogue JA, Sosa JA. A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors. PLoS One 2016 Aug 18 [PMID: 27537691] (ICC/IF)
• Koh J, Hogue JA, Wang Y et al. Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism. J. Cell. Mol. Med. 2015 Dec 05 [PMID: 26638194] (ICC/IF, Human)
• Shilo V, Ben-Dov IZ, Nechama M et al. Parathyroid-specific deletion of dicer-dependent microRNAs abrogates the response of the parathyroid to acute and chronic hypocalcemia and uremia FASEB J. 2015 Jun 08 [PMID: 26054367] (IHC-P, Mouse)
• Szalat A, Shahar M, Shpitzen S et al. Calcium-sensing receptor sequencing in 21 patients with idiopathic or familial parathyroid disorder: pitfalls and characterization of a novel I32 V loss-of-function mutation. Endocrine. 2014 Aug 05 [PMID: 25091521]
• Olauson H, Lindberg K, Amin R et al. Parathyroid-Specific Deletion of Klotho Unravels a Novel Calcineurin-Dependent FGF23 Signaling Pathway That Regulates PTH Secretion. PLoS Genet 2013 Dec [PMID: 24348262] (IHC-P, ICC/IF, Mouse)
• Walter S, Baruch A, Dong J et al. Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the calcium-sensing receptor, for the treatment of secondary hyperparathyroidism in hemodialysis patients. J Pharmacol Exp Ther. 2013 Aug [PMID: 23674604]
• Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the Calcium-Sensing Receptor in the Kidney Causes Parathyroid Hormone-Independent Hypocalciuria J Am Soc Nephrol 2012 Sep 20 [PMID: 22997254]
• Galitzer H, Ben-Dov IZ, Silver J et al. Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int. 2010 Feb [PMID: 20016468] (IHC-P, Rat)
• Meir T, Levi R, Lieben L et al. Deletion of the vitamin D receptor specifically in the parathyroid demonstrates a limited role for the receptor in parathyroid physiology. Am J Physiol Renal Physiol;297(5):F1192-8. 2009 Nov. [PMID: 19692484] (IHC-P, Mouse)
• Alam MU et al. Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells. Cardiovasc Res;81(2):260-8. 2009 Feb 1. [PMID: 18852253] (WB, IHC)
• Weston AH et al. Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231. Circ Res;97(4):391-8. 2005 Aug 19. [PMID: 16037572] (IHC)
• Goldsmith PK et al. Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor. J Biol Chem. 274(16):11303-9. 1999 Apr 16. [PMID: 10196220] (WB)
• Goldsmith PK et al. Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition. J Bone Miner Res;12(11):1780-8. 1997 Nov. [PMID: 9383682] (ELISA, FLOW)

FAQs for Calcium-sensing R/CaSR Antibody (NB120-19347). (Showing 1 - 1 of 1 FAQs).

1. Do you have a blocking peptide for NB120-19347?
   • Unfortunately, no, the blocking peptide for the this antibody is not currently available.

Bioinformatics

• Gene Symbol: CASR
• Entrez:
  • 846(Human)
  • 24247(Rat)
• Uniprot:
  • P41180(Rat)