Calcium-sensing R/CaSR Antibody (5C10, ADD)

Images

 
Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of calcium sensing receptor in HEK7-2 cell extract.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue extracts.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue.
Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody. Fresh stomach tissue was fixed in 4% formalin for 1 ...read more
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human brain tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
Clone
5C10, ADD
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Calcium-sensing R/CaSR Antibody (5C10, ADD) Summary

Immunogen
Synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CASR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunofluorescence 2 ug/ml
Application Notes
Flow usage was reported in scientific literature (PMID: 9383682). IHC-P usage was reported in scientific literature. WB: Detects an approx. 130 and 150 kDa.
Positive Control
Calcium-sensing R/CaSR Lysate (NBL1-08721)
Publications
Read Publications using
NB120-19347 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Calcium-sensing R/CaSR Antibody (5C10, ADD)

  • calcium-sensing receptor
  • CAR
  • CaSR
  • EIG8
  • extracellular calcium-sensing receptor
  • FHH
  • FIH
  • GPRC2A
  • GPRC2AHHC
  • HHC1
  • hypocalciuric hypercalcemia 1
  • NSHPT
  • parathyroid Ca(2+)-sensing receptor 1
  • Parathyroid cell calcium-sensing receptor
  • PCaR1
  • PCAR1MGC138441

Background

The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF

Publications for Calcium-sensing R/CaSR Antibody (NB120-19347)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC, IHC-P, WB.


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ELISA
(1)
FLOW
(1)
ICC/IF
(3)
IHC
(2)
IHC-P
(4)
WB
(3)
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Human
(1)
Mouse
(4)
Rat
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB120-19347 Applications Species
Boudot C, Henaut L, Thiem U et al. Overexpression of a functional calcium-sensing receptor dramatically increases osteolytic potential of MDA-MB-231 cells in a mouse model of bone metastasis through epiregulin-mediated osteoprotegerin downregulation Oncotarget 2017 Aug 22 [PMID: 28915604] (WB, Mouse) WB Mouse
Dai XQ, Perez PL, Soria G et al. External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells. Exp. Cell Res. Nov 9 2016 [PMID: 27836810] (Porcine) Porcine
Koh J, Hogue JA, Sosa JA. A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors. PLoS One 2016 Aug 18 [PMID: 27537691] (ICC/IF) ICC/IF
Koh J, Hogue JA, Wang Y et al. Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism. J. Cell. Mol. Med. 2015 Dec 05 [PMID: 26638194] (ICC/IF, Human) ICC/IF Human
Shilo V, Ben-Dov IZ, Nechama M et al. Parathyroid-specific deletion of dicer-dependent microRNAs abrogates the response of the parathyroid to acute and chronic hypocalcemia and uremia FASEB J. 2015 Jun 08 [PMID: 26054367] (IHC-P, Mouse) IHC-P Mouse
Szalat A, Shahar M, Shpitzen S et al. Calcium-sensing receptor sequencing in 21 patients with idiopathic or familial parathyroid disorder: pitfalls and characterization of a novel I32 V loss-of-function mutation. Endocrine. 2014 Aug 05 [PMID: 25091521]
Olauson H, Lindberg K, Amin R et al. Parathyroid-Specific Deletion of Klotho Unravels a Novel Calcineurin-Dependent FGF23 Signaling Pathway That Regulates PTH Secretion. PLoS Genet 2013 Dec [PMID: 24348262] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF Mouse
Walter S, Baruch A, Dong J et al. Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the calcium-sensing receptor, for the treatment of secondary hyperparathyroidism in hemodialysis patients. J Pharmacol Exp Ther. 2013 Aug [PMID: 23674604]
Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the Calcium-Sensing Receptor in the Kidney Causes Parathyroid Hormone-Independent Hypocalciuria J Am Soc Nephrol 2012 Sep 20 [PMID: 22997254]
Galitzer H, Ben-Dov IZ, Silver J et al. Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int. 2010 Feb [PMID: 20016468] (IHC-P, Rat) IHC-P Rat
Show All 15 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calcium-sensing R/CaSR Antibody (NB120-19347). (Showing 1 - 1 of 1 FAQs).

  1. Do you have a blocking peptide for NB120-19347?
    • Unfortunately, no, the blocking peptide for the this antibody is not currently available.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASR
Entrez
Uniprot