Calcium-sensing R/CaSR Antibody (5C10, ADD)

Images

 
Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.
Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of calcium sensing receptor in HEK7-2 cell extract.
Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody. Fresh stomach tissue was fixed in 4% formalin for 1 ...read more
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human brain tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
5C10, ADD
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Calcium-sensing R/CaSR Antibody (5C10, ADD) Summary

Immunogen
Partial synthetic peptide corresponding to residues 214 - 235 of human calcium sensing receptor R / CaSR [UniProt P41180]
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CASR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
IHC-P usage was reported in scientific literature. In Western blot this antibody detects bands at approximately 130 and 150 kDa. We have conflicting data on the use of this antibody in Flow.
Control
Calcium-sensing R/CaSR Overexpression Lysate
Publications
Read Publications using
NB120-19347 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Calcium-sensing R/CaSR Antibody (5C10, ADD)

  • calcium-sensing receptor
  • CAR
  • CaSR
  • EIG8
  • extracellular calcium-sensing receptor
  • FHH
  • FIH
  • GPRC2A
  • GPRC2AHHC
  • HHC1
  • hypocalciuric hypercalcemia 1
  • NSHPT
  • parathyroid Ca(2+)-sensing receptor 1
  • Parathyroid cell calcium-sensing receptor
  • PCaR1
  • PCAR1MGC138441

Background

The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61816
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
PP-N4111-00
Species: Hu
Applications: WB, IHC, IP
AF7356
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP1-88192
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01800
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-128
Species: Hu, Mu, Rt
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-92637
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-66778
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB400-153
Species: Hu, Mu
Applications: WB, ICC/IF
AF1107
Species: Mu
Applications: WB, IHC
H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA

Publications for Calcium-sensing R/CaSR Antibody (NB120-19347)(20)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: ELISA, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
Flow
(2)
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(6)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(6)
Rat
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB120-19347 Applications Species
Nery F, Siranosian J, Rosales I et al. Impaired kidney structure and function in spinal muscular atrophy Neurol Genet Oct 1 2019 [PMID: 31517062] (IHC-P, Human) IHC-P Human
Bachinger D, Egli H, Goosmann MM et al. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear Cell Tissue Res. Jul 23 2019 [PMID: 31338584] (IHC-P, Mouse) IHC-P Mouse
Luchsinger LL, Strikoudis A, Danzl NM et al. Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro Cell Stem Cell 2019 May 31 [PMID: 31178255] (Flow, Mouse, Human) Flow Mouse, Human
Issa H, Henaut L, Abdallah JB et al. Activation of the calcium-sensing receptor in human valvular interstitial cells promotes calcification J. Mol. Cell. Cardiol. Feb 12 2019 [PMID: 30769016] (WB, IHC-Fr, Human) WB, IHC-Fr Human
Frees S, Breuksch I, Haber T et al. Calcium-sensing receptor (CaSR) promotes development of bone metastasis in renal cell carcinoma. Oncotarget. [PMID: 29644008] (Flow, WB, Human) Flow, WB Human
Boudot C, Henaut L, Thiem U et al. Overexpression of a functional calcium-sensing receptor dramatically increases osteolytic potential of MDA-MB-231 cells in a mouse model of bone metastasis through epiregulin-mediated osteoprotegerin downregulation Oncotarget 2017 Aug 22 [PMID: 28915604] (WB, Mouse) WB Mouse
Dai XQ, Perez PL, Soria G et al. External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells. Exp. Cell Res. Nov 9 2016 [PMID: 27836810] (Porcine) Porcine
Koh J, Hogue JA, Sosa JA. A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors. PLoS One 2016 Aug 18 [PMID: 27537691] (ICC/IF) ICC/IF
Koh J, Hogue JA, Wang Y et al. Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism. J. Cell. Mol. Med. 2015 Dec 05 [PMID: 26638194] (ICC/IF, Human) ICC/IF Human
Shilo V, Ben-Dov IZ, Nechama M et al. Parathyroid-specific deletion of dicer-dependent microRNAs abrogates the response of the parathyroid to acute and chronic hypocalcemia and uremia FASEB J. 2015 Jun 08 [PMID: 26054367] (IHC-P, Mouse) IHC-P Mouse
Show All 20 Publications.

Reviews for Calcium-sensing R/CaSR Antibody (NB120-19347) (0)

There are no reviews for Calcium-sensing R/CaSR Antibody (NB120-19347). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calcium-sensing R/CaSR Antibody (NB120-19347). (Showing 1 - 1 of 1 FAQs).

  1. Do you have a blocking peptide for NB120-19347?
    • Unfortunately, no, the blocking peptide for the this antibody is not currently available.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-19347AF350
Alexa Fluor 405 NB120-19347AF405
Alexa Fluor 488 NB120-19347AF488
Alexa Fluor 532 NB120-19347AF532
Alexa Fluor 594 NB120-19347AF594
Alexa Fluor 647 NB120-19347AF647
Alexa Fluor 700 NB120-19347AF700
Alexa Fluor 750 NB120-19347AF750
Biotin NB120-19347B
DyLight 350 NB120-19347UV
DyLight 405 NB120-19347V
DyLight 488 NB120-19347G
DyLight 550 NB120-19347R
DyLight 594 NB120-19347DL594
DyLight 650 NB120-19347C
DyLight 680 NB120-19347FR
DyLight 755 NB120-19347IR
FITC NB120-19347F
HRP NB120-19347H
Janelia Fluor 549 NB120-19347JF549
Janelia Fluor 646 NB120-19347JF646

Additional Calcium-sensing R/CaSR Products

Bioinformatics Tool for Calcium-sensing R/CaSR Antibody (NB120-19347)

Discover related pathways, diseases and genes to Calcium-sensing R/CaSR Antibody (NB120-19347). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Calcium-sensing R/CaSR Antibody (NB120-19347)

Discover more about diseases related to Calcium-sensing R/CaSR Antibody (NB120-19347).
 

Pathways for Calcium-sensing R/CaSR Antibody (NB120-19347)

View related products by pathway.

PTMs for Calcium-sensing R/CaSR Antibody (NB120-19347)

Learn more about PTMs related to Calcium-sensing R/CaSR Antibody (NB120-19347).

Blogs on Calcium-sensing R/CaSR

There are no specific blogs for Calcium-sensing R/CaSR, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calcium-sensing R/CaSR Antibody (5C10, ADD) and receive a gift card or discount.

Bioinformatics

Gene Symbol CASR
Entrez
Uniprot