Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
IHC-P usage was reported in scientific literature. In Western blot this antibody detects bands at approximately 130 and 150 kDa. We have conflicting data on the use of this antibody in Flow.
Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified
Alternate Names for Calcium-sensing R/CaSR Antibody (5C10, ADD)
calcium-sensing receptor
CAR
CaSR
EIG8
extracellular calcium-sensing receptor
FHH
FIH
GPRC2A
GPRC2AHHC
HHC1
hypocalciuric hypercalcemia 1
NSHPT
parathyroid Ca(2+)-sensing receptor 1
Parathyroid cell calcium-sensing receptor
PCaR1
PCAR1MGC138441
Background
The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Reviews for Calcium-sensing R/CaSR Antibody (NB120-19347) (0)
There are no reviews for Calcium-sensing R/CaSR Antibody (NB120-19347).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
View specific protocols for Calcium-sensing R/CaSR Antibody (NB120-19347):
Bioinformatics Tool for Calcium-sensing R/CaSR Antibody (NB120-19347)
Discover related pathways, diseases and genes to Calcium-sensing R/CaSR Antibody (NB120-19347). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Calcium-sensing R/CaSR Antibody (NB120-19347)
Discover more about diseases related to Calcium-sensing R/CaSR Antibody (NB120-19347).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Calcium-sensing R/CaSR Antibody (5C10, ADD) and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.