Western Blot: FOXP3 Antibody [NB600-245] - Detection of human FOXP3 using NB 600-245. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector
Immunocytochemistry/ Immunofluorescence: FOXP3 Antibody [NB600-245] - Incubated only with CD4 and no Foxp3
Immunocytochemistry/ Immunofluorescence: FOXP3 Antibody [NB600-245] - Sample: Healthy thymus from a C57BL/6 mouse
This FOXP3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. By Western blot, this antibody recognizes bands at ~47.2 kDa representing FOXP3. For IHC-Fr it has been used in mouse thymus from an influenza-infected mouse or thymus from healthy mice (C57BL/6). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
47.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FOXP3 (forkhead box protein P3) belongs to forkhead/winged-helix gene family of transcription factors that regulates the development and function of immune system. Localized in the nucleus, FOXP3 interacts with IKZF3 and is a crucial factor for the generation of CD4+CD25+ regulatory T cells (Tregs) and high levels expression of FOXP3 is essential for Treg's suppressive functions. SIRT1 facilitates FOXP3' decetylation, whereas, its acetylation on lysine residues causes its stabilization and promotes differentiation of T-cells into induced Tregs. Mutations of the FOXP3 gene lead to X-linked autoimmunity-allergic dysregulation syndrome in humans (also called immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome or IPEX) and the lymphoproliferative disease in Scurfy mouse. The FOXP3 X-linked recessive mutation results in lethality in hemizygous male mice soon after birth, and is characterized by excessive proliferation of CD4+ T cells, extensive multi-organ infiltration by leukocytes and systemic elevation of numerous cytokines. FoxP3 has also been shown to function as a tumor suppressor in several types of cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively,... Read full blog post.