FoxP3 Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

FoxP3 Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This FOXP3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. By Western blot, this antibody recognizes bands at ~47.2 kDa representing FOXP3. For IHC-Fr it has been used in mouse thymus from an influenza-infected mouse or thymus from healthy mice (C57BL/6).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. This antibody does not appear to detect the mouse FOXP3 protein by western blot, but does work on mouse tissue for IHC (see data below).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FoxP3 Antibody [DyLight 405]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (forkhead box protein P3) belongs to forkhead/winged-helix gene family of transcription factors that regulates the development and function of immune system. Localized in the nucleus, FOXP3 interacts with IKZF3 and is a crucial factor for the generation of CD4+CD25+ regulatory T cells (Tregs) and high levels expression of FOXP3 is essential for Treg's suppressive functions. SIRT1 facilitates FOXP3' decetylation, whereas, its acetylation on lysine residues causes its stabilization and promotes differentiation of T-cells into induced Tregs. Mutations of the FOXP3 gene lead to X-linked autoimmunity-allergic dysregulation syndrome in humans (also called immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome or IPEX) and the lymphoproliferative disease in Scurfy mouse. The FOXP3 X-linked recessive mutation results in lethality in hemizygous male mice soon after birth, and is characterized by excessive proliferation of CD4+ T cells, extensive multi-organ infiltration by leukocytes and systemic elevation of numerous cytokines. FoxP3 has also been shown to function as a tumor suppressor in several types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB600-245V). (Showing 1 - 3 of 3 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the immunogen sequence of this FoxP3 antibody?
    • A synthetic sequence of amino acids 400 - 431 within the C-terminal region of Human FoxP3.

Secondary Antibodies

 

Isotype Controls

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Blogs on FoxP3.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively,...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Bioinformatics

Gene Symbol FOXP3
Entrez