Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Image from product review ...read more
Orthogonal Strategies: Dual RNAscope ISH-IHC: CD11b Antibody [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; ...read more
Immunohistochemistry: CD11b Antibody [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), ...read more
Flow Cytometry: CD11b Antibody [NB110-89474] - A surface stain was performed on Raw264.7 cells with DyLight 550-conjugated [NB110-89474R] (blue) and a matched isotype control (orange). Cells were incubated in an ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody [NB110-89474] - CD11b antibody [NB110-89474] was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
Immunohistochemistry: CD11b Antibody [NB110-89474] - Representative photomicrographs of cross sections of uteri collected from wild type (WT) mice 24 h after intrauterine infusion with 100 ul of vehicle or 100 ug ...read more
Simple Western: CD11b Antibody [NB110-89474] - Specific band for Cd11b in 1.0 mg/mL of Dentate Gyrus from Rat Brain. [NB110-89474] was used at a dilution of 1:50. This experiment was performed under reducing conditions ...read more
Western Blot: CD11b Antibody [NB110-89474] - Western Blot detection of CD11b in RAW 264.7 whole cell lysates using [NB110-89474].
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Immunohistochemical analysis of frozen sections of mouse spleen using [NB110-89474]. Image from verified customer review.
Immunohistochemistry: CD11b Antibody [NB110-89474] - Immunohistochemical analysis of CD11b in human renal cancer with [NB110-89474] using 3,3'-Diaminobenzidine (DAB) with hematoxylin counterstain.
This CD11b antibody is useful for WB analysis (PMID 31082627), ICC, Flow Cytometry (PMID 21422470) and IHC-P (PMID 31022918) embedded sections. In WB a specific band is observed ~160 kDa and an apparant non-specific band is observed ~56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human samples with WB. Use in IHC-Fr and ICC/IF reported in scientific literature (PMID 23980916). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in In-situ Hybridization reported in scientific literature (PMID: 27133471).
127.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Monkey reactivity reported in scientific literature (PMID: 26443820). Rhesus Macaque reactivity reported in scientific literature (PMID: 29760177). Use in C. elegans reported in scientific literature (PMID:32058942).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for CD11b Antibody
antigen CD11b (p170)
CD11 antigen-like family member B
Cell surface glycoprotein MAC-1 subunit alpha
Complement component 3 receptor 3 subunit
CR-3 alpha chain
Integrin alpha M
integrin, alpha M (complement component 3 receptor 3 subunit)
Leukocyte adhesion receptor MO1
Mac1, alpha subunit
macrophage antigen alpha polypeptide
Mo1, alpha subunit
neutrophil adherence receptor alpha-M subunit
Neutrophil adherence receptor
CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S.,... Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S.,... Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Yew M, Koh R, Chye S et al. Edible birds nest improves motor behavior and protects dopaminergic neuron against oxidative and nitrosative stress in Parkinsons disease mouse model Journal of Functional Foods Sep 1 2018 (IHC, Mouse)
Lerbs T, Cui L, King M et al. CD47 prevents the elimination of diseased fibroblasts in scleroderma bioRxiv Jun 7 2020 (IHC, Human)
Goksu Erol A, Aklncl E, Kocancl F et al. Masitinib mediates TGF-Beta1 and Nitric Oxide Secretion and Ameliorates MPTP/Microglia-Induced Degeneration of Differentiated SH-SY5Y Cells bioRxiv Jul 17 2020 (ICC/IF, Human)
Ohshima R, Hotsumi K. Age-related decrease in glucagon-like peptide-1 in mouse prefrontal cortex but not in hippocampus despite the preservation of its receptor American Journal of BioScience. 2015-02-29 (IHC-Fr, Mouse)
Human lung tissue was stained with CD11b. Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
Lung collected from an IL1b-KO monocytes-treated recipient was stained with CD11b. Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
FAQs for CD11b Antibody (NB110-89474). (Showing 1 - 8 of 8 FAQs).
I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
What types of cells can this CD11b antibody be used as a marker for?
A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
The protocol is referencting the PIER method using Proteinase K as the enzyme.
I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
Is CD11b antibody also staining neutrophils in the brain
CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
CD11b - More than a microglial marker The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.