CD11b Antibody

Images

 
Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using CD11b antibody at 1:400 dilution. Nuclei counterstained with DAPI (blue). Image from product review by verified customer.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody [NB110-89474] - CD11b antibody was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Simple Western: CD11b Antibody [NB110-89474] - Specific band for Cd11b in 1.0 mg/mL of Dentate Gyrus from Rat Brain. Antibody at 1:50. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: CD11b Antibody [NB110-89474] - Detection of CD11b in RAW 264.7 whole cell lysates using NB110-89474.
Immunohistochemistry: CD11b Antibody [NB110-89474] - Analysis of CD11b in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Mouse spleen. Image from verified customer review.
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Blue: DAPI+ nuclei. Red: CD11b. Lung collected from an IL1b-KO monocytes-treated recipient was stained with CD11b antibody (1:100), diluted in a blocking ...read more
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Blue: DAPI+ nuclei Red: CD11b Human lung tissue was stained with CD11b antibody (1:100), diluted in a blocking buffer. A secondary Alexa Fluor 647-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CD11b Antibody Summary

Immunogen
A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Localization
Monocytes and granulocytes.
Marker
Microglia Marker, Myeloid Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 1:50
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
  • Single Cell Western 1:10
Application Notes
This CD11b antibody is useful for WB analysis, ICC, Flow Cytometry (PMID 21422470) and IHC-P embedded sections. In WB a specific band is observed ~160 kDa and an apparant non-specific band is observed ~56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human samples with WB. Use in IHC-Fr reported in scientific literature (PMID 23980916). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raw 264.7 Lysate (NBP1-30158)
Reviewed Applications
Read 9 Reviews rated 4.7
using
NB110-89474 in the following applications:

Publications
Read Publications using
NB110-89474 in the following applications:

Reactivity Notes

Sheep (88%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research.

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD11b Antibody

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • CD11b
  • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
  • Cell surface glycoprotein MAC-1 subunit alpha
  • CR-3 alpha chain
  • CR3AMGC117044
  • Integrin alpha M
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • ITGAM
  • Leukocyte adhesion receptor MO1
  • MAC 1
  • MAC1
  • MAC-1
  • Mac1, alpha subunit
  • MAC1A
  • Mac-1a
  • macrophage antigen alpha polypeptide
  • Mo1, alpha subunit
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. This CD11B antibody was used to identify granulocytes in mouse uterine lining(1) in IHC staining. A Scripps institute study used the CD11B antibody to look at the effect of elevated IL-6 levels on the expression of cellular and synaptic proteins in hippocampal samples where CD11B was considered an indicator of overall expression levels (2). Another study used the CD11B antibody as a marker of microglia cells to look at the effect of increased CCL2 expression in transgenic mice on synaptic transmission in the hippocampus (3).
PMIDs: 1. 20877575 Staining of Granulocytes Using CD11B antibody.
2. 22609298 IHC Staining of Hippocampus Sections Using CD11B Antibody.
3. 21356306 Quantitative WB Using CD11B Antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11b Antibody (NB110-89474)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(7)
IHC
(6)
IHC-Fr
(3)
IHC-P
(5)
WB
(8)
All Applications
Filter By Species
Human
(5)
Mouse
(21)
Rat
(5)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NB110-89474 Applications Species
Flores-Martinez A, Garcia-Nunez A, Rojas A, Cano DA. Stabilization of HIF-2a impacts pancreas growth. Sci Rep Sep 12 2018 [PMID: 30209343] (IHC, Mouse) IHC Mouse
Anderton H, Bandala-Sanchez E, Simpson DS et al. RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development. Cell Death Differ. Sep 5 2018 [PMID: 30185824] (IHC-P, Mouse) IHC-P Mouse
Xu Y, Xu J, Ge K et al. Anti-inflammatory effect of low molecular weight fucoidan from Saccharina japonica on atherosclerosis in apoE-knockout mice Int. J. Biol. Macromol. Jun 12 2018 [PMID: 29906534] (IHC, Mouse) IHC Mouse
Periyasamy P, Thangaraj A, Guo ML et al. Epigenetic promoter DNA methylation of miR-124 promotes HIV-1 Tat-mediated microglial activation via MECP2-STAT3 axis J. Neurosci. May 14 2018 [PMID: 29760177] (Mouse) Mouse
Schmidt ML, Hobbing KR, Donninger H, Clark GJ. RASSF1A Deficiency Enhances RAS-Driven Lung Tumorigenesis Cancer Res. May 15 2018 [PMID: 29735543] (Human) Human
Dorsam B, Seiwert N, Foersch S et al. PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression. Proc. Natl. Acad. Sci. U.S.A. Apr 24 2018 [PMID: 29632181] (ICC/IF, Mouse) ICC/IF Mouse
Nurnberg C, Kociok N, Brockmann C et al. Dataset on the activation of Muller cells through macrophages upon hypoxia in the retina Data in Brief [PMID: 29255783] (Mouse) Mouse
Li Y, Lu B, Sheng L et al. Hexokinase 2-dependent Hyperglycolysis Driving Microglial Activation Contributes to Ischemic Brain Injury. J. Neurochem. 2017 Dec 05 [PMID: 29205357] (Rat) Rat
Baranova IN, Souza ACP, Bocharov AV et al. Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo. PLoS ONE. Apr 19 2017 [PMID: 28423002] (ICC/IF, Human) ICC/IF Human
Kim So Young, Kang Dongxu, Choi Hye Jin et al. Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-b2 induces anti-tumor immune activation. Oncotarget 2017 [PMID: 28178658] (IHC, Mouse) IHC Mouse
Show All 36 Publications.

Reviews for CD11b Antibody (NB110-89474) (9) 4.79

Average Rating: 4.7
(Based on 9 reviews)
We have 9 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-89474:
Filter by Applications
WB
(3)
IHC-Fr
(3)
IHC-P
(1)
IF
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
Western Blot CD11b NB110-89474
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reviewed by:
Anonymous
WB Mouse 11/30/2018
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Summary

ApplicationWestern Blot
Sample TestedAdult mouse brain tissue
SpeciesMouse
LotY-7
Western Blot CD11b NB110-89474
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reviewed by:
Anonymous
WB Human 10/28/2018
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Summary

ApplicationWestern Blot
Sample TestedJurkat and THP1 cells
SpeciesHuman
LotY-5
Immunohistochemistry-Frozen CD11b NB110-89474
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reviewed by:
Sasan Jalili
IHC-Fr Mouse 05/12/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen mouse spleen
SpeciesMouse
LotNB110-89474
Immunohistochemistry-Frozen CD11b NB110-89474
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reviewed by:
Jim Hsiao
IHC-Fr Human 02/22/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHuman lung graft
SpeciesHuman
LotY-2

Comments

CommentsHuman lung tissue was stained with CD11b.
Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
Immunohistochemistry-Frozen CD11b NB110-89474
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reviewed by:
Jim Hsiao
IHC-Fr Mouse 02/22/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLung graft,IL1b-KO monocyte treated lung
SpeciesMouse
LotY-2

Comments

CommentsLung collected from an IL1b-KO monocytes-treated recipient was stained with CD11b.
Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
Immunohistochemistry-Paraffin CD11b NB110-89474
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reviewed by:
Anonymous
IHC-P Mouse 09/15/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse spleen cells
SpeciesMouse
LotX-8

Comments

CommentsPermeabillized with 3% Triton-X100
Antigen Retrieval with Citrate buffer, 20 min, 90C
1st ab dilution: 1:200
Immunofluorescence CD11b NB110-89474
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reviewed by:
Anonymous
IF Mouse 11/29/2016
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Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsIn order to visualize staining the secondary method was used. Blocking was performed in goat serum and secondary antibodies used were Alexa Fluor 488 conjugate.
Western Blot CD11b NB110-89474
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reviewed by:
Anonymous
WB 01/21/2015
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Summary

ApplicationWestern Blot
Immunofluorescence CD11b NB110-89474
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reviewed by:
Dawn Jaquish
IF Mouse 03/05/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Pancreas
SpeciesMouse
CommentsThe antibody worked well at the recommended dilution for our research

Blocking

Blocking Details10% Goat serum + 5% BSA in TBS-T for 1 hr at RT. I also labeled for the nuclei with DAPI by 5min at RT in TBS

Primary Anitbody

Dilution RatioAntibody used at 1:400 dilution overnight at 4oC in TBS-T

Secondary Antibody

Secondary DescriptionGoat anti Rabbit Alexa 594
Secondary Manufacturer Cat#Invitrogen
Secondary Concentration1:500 Dilution

Details

Detection NotesDetection of signal was done by fluorescence and the negative control was normal rabbit IgG at the same concentration
Fixation DetailsFixed overnight with 10% NBF. Antigen Retrieval done for 20 min.
Wash Description3X 5min washes in TBS-T

Comments

CommentsThe antibody worked well at the recommended dilution for our research

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD11b Antibody (NB110-89474). (Showing 1 - 7 of 7 FAQs).

  1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
    • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
  2. What types of cells can this CD11b antibody be used as a marker for?
    • A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
  3. Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 
    • This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
  4. Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
    • I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
  5. We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
    • We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
  6. The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
    • The protocol is referencting the PIER method using Proteinase K as the enzyme.
  7. I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
    • We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-89474AF405
Alexa Fluor 488 Labeled NB110-89474AF488
Alexa Fluor 647 Labeled NB110-89474AF647
Alexa Fluor 700 Labeled NB110-89474AF700
Biotin Labeled NB110-89474B
DyLight 405 Labeled NB110-89474V
DyLight 488 Labeled NB110-89474G
DyLight 550 Labeled NB110-89474R
DyLight 650 Labeled NB110-89474C
FITC Labeled NB110-89474F
HRP Labeled NB110-89474H
PE Labeled NB110-89474PE

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Blogs on CD11b.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

CD11b - More than a microglial marker
The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

The CD11b Antibody: A Marker for Microglial Cells
Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac...  Read full blog post.

CD11b Expression, Leukocyte Adhesion and the Innate Immune System
CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen...  Read full blog post.

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Recent Reviews

4.7
5
7
4
1
3
1
2
0
1
0

Anonymous
11/30/2018
Application: WB
Species: Mouse

Anonymous
10/28/2018
Application: WB
Species: Human

Sasan Jalili
05/12/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ITGAM
Entrez
Uniprot