Immunocytochemistry/ Immunofluorescence: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using CD11b antibody at 1:400 dilution. Nuclei counterstained with DAPI (blue). Image provided by product review by ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody [NB110-89474] - CD11b antibody was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Simple Western: CD11b Antibody [NB110-89474] - Simple Western lane view shows a specific band for Cd11b in 1.0 mg/ml of Dentate Gyrus from Rat Brain at an antibody concentration of 1:50. This experiment was performed ...read more
Western Blot: CD11b Antibody [NB110-89474] - Detection of CD11b in RAW 264.7 whole cell lysates using NB110-89474.
Immunohistochemistry: CD11b Antibody [NB110-89474] - Analysis of CD11b in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Blue: DAPI+ nuclei. Red: CD11b. Lung collected from an IL1b-KO monocytes-treated recipient was stained with CD11b. Primary antibody (1:100) was diluted in a ...read more
Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Blue: DAPI+ nucleiRed: CD11bHuman lung tissue was stained with CD11b. Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary ...read more
Hu, Mu, Rt, BvSpecies Glossary
WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
127 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for CD11b Antibody
antigen CD11b (p170)
CD11 antigen-like family member B
CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
Cell surface glycoprotein MAC-1 subunit alpha
CR-3 alpha chain
Integrin alpha M
integrin, alpha M (complement component 3 receptor 3 subunit)
Leukocyte adhesion receptor MO1
Mac1, alpha subunit
macrophage antigen alpha polypeptide
Mo1, alpha subunit
neutrophil adherence receptor alpha-M subunit
Neutrophil adherence receptor
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. This CD11B antibody was used to identify granulocytes in mouse uterine lining(1) in IHC staining. A Scripps institute study used the CD11B antibody to look at the effect of elevated IL-6 levels on the expression of cellular and synaptic proteins in hippocampal samples where CD11B was considered an indicator of overall expression levels(2). Another study used the CD11B antibody as a marker of microglia cells to look at the effect of increased CCL2 expression in transgenic mice on synaptic transmission in the hippocampus(3). PMIDs: 1. 20877575 Staining of Granulocytes Using CD11B antibody 2. 22609298 IHC Staining of Hippocampus Sections Using CD11B Antibody 3. 21356306 Quantitative WB Using CD11B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Ohshima R, Hotsumi K. Age-related decrease in glucagon-like peptide-1 in mouse prefrontal cortex but not in hippocampus despite the preservation of its receptor American Journal of BioScience. 2015-02-29 (IHC-Fr, Mouse)
Human lung tissue was stained with CD11b. Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
Lung collected from an IL1b-KO monocytes-treated recipient was stained with CD11b. Primary antibody (1:100) was diluted in a blocking buffer (PBS with 0.5%). A secondary Alexa647-conjugated Goat anti-Rabbit antibody (1:400) was used to detect CD11b.
FAQs for CD11b Antibody (NB110-89474). (Showing 1 - 7 of 7 FAQs).
I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
What types of cells can this CD11b antibody be used as a marker for?
A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
The protocol is referencting the PIER method using Proteinase K as the enzyme.
I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
CD11b - More than a microglial marker The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the... Read full blog post.
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