CD11c Antibody (AP-MAB0806)

Images

 
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Mouse spleen tissue.
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Negative control, mouse spleen tissue section.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
AP-MAB0806
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
LYOPH

CD11c Antibody (AP-MAB0806) Summary

Immunogen
This CD11c antibody was produced from a hybridoma (mouse myeloma fused with spleen cells)from an Armenian Hamster immunized with mouse lymphocytes.
Marker
Dendritic Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
ITGAX
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:25-1:100
  • Immunohistochemistry-Paraffin 1:25-1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
4% PFA fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse CD11c. This antibody is CyTOF ready. Use in ICC/IF reported in scientific literature (PMID: 30300579).
Publications
Read Publications using
NB110-97871 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute the antibody with 200 ul sterile PBS (final concentration 500ug/ml). Reconstituted antibody can be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity.

Alternate Names for CD11c Antibody (AP-MAB0806)

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (NB110-97871)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-P
(1)
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Filter By Species
Mouse
(5)
All Species

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Product General Protocols

View specific protocols for CD11c Antibody (NB110-97871): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD11c Antibody (NB110-97871). (Showing 1 - 1 of 1 FAQs).

  1. For the following product, please let us know the lyophilized buffer composition. Is it only PBS?
    • This antibody (NB110-97871) is lyophilized and the lyophilized product only contains the antibody and it has to be reconstituted in PBS.

Secondary Antibodies

 

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Blogs on CD11c.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Bioinformatics

Gene Symbol ITGAX
Entrez
Uniprot