Aminopeptidase N/CD13 Antibody [Unconjugated]

Images

 
Aminopeptidase N/CD13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 15 µg/mL for 3 hours at room ...read more
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 0.1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Aminopeptidase N/CD13 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase N/CD13
Lys69-Ser966
Accession # P97449
Specificity
Detects mouse Aminopeptidase N/CD13 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANPEP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2335 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody [Unconjugated]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

  1. Chen, H. et al. (1996) J. Immunol. 157:2593.
  2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
  3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
  4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aminopeptidase N/CD13 Antibody (AF2335)(16)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ICC
(1)
IHC
(11)
IHC-Fr
(3)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(16)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF2335 Applications Species
MR Williamson, CJA Fuertes, AK Dunn, MR Drew, TA Jones Reactive astrocytes facilitate vascular repair and remodeling after stroke Cell Reports, 2021;35(4):109048. 2021 [PMID: 33910014] (IHC, Mouse) IHC Mouse
Y Wu, H Wu, J Zeng, B Pluimer, S Dong, X Xie, X Guo, T Ge, X Liang, S Feng, Y Yan, JF Chen, N Sta Maria, Q Ma, F Gomez-Pini, Z Zhao Mild traumatic brain injury induces microvascular injury and accelerates Alzheimer-like pathogenesis in mice Acta neuropathologica communications, 2021;9(1):74. 2021 [PMID: 33892818] (IHC, Mouse) IHC Mouse
S Grubb, C Cai, BO Hald, L Khennouf, RP Murmu, AGK Jensen, J Fordsmann, S Zambach, M Lauritzen Precapillary sphincters maintain perfusion in the cerebral cortex Nat Commun, 2020;11(1):395. 2020 [PMID: 31959752] (IHC, Mouse) IHC Mouse
RM Rajani, J Ratelade, V Domenga-De, Y Hase, H Kalimo, RN Kalaria, A Joutel Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL Acta Neuropathol Commun, 2019;7(1):187. 2019 [PMID: 31753008] (IHC, Mouse) IHC Mouse
DY Chen, NH Sun, YP Lu, LJ Hong, TT Cui, CK Wang, XH Chen, SS Wang, LL Feng, WX Shi, K Fukunaga, Z Chen, YM Lu, F Han GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury Theranostics, 2019;9(20):5937-5955. 2019 [PMID: 31534530] (IHC, Mouse) IHC Mouse
A Ayyaz, S Kumar, B Sangiorgi, B Ghoshal, J Gosio, S Ouladan, M Fink, S Barutcu, D Trcka, J Shen, K Chan, JL Wrana, A Gregorieff Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell Nature, 2019;0(0):. 2019 [PMID: 31019301] (IHC, Mouse) IHC Mouse
V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019;10(1):518. 2019 [PMID: 30705270] (IHC, Mouse) IHC Mouse
S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019;26(5):1227-1241.e6. 2019 [PMID: 30699351] (IHC, Mouse) IHC Mouse
Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism Mol Neurodegener, 2018;13(1):57. 2018 [PMID: 30340601] (IHC, Mouse) IHC Mouse
K Koizumi, Y Hattori, SJ Ahn, I Buendia, A Ciacciarel, K Uekawa, G Wang, A Hiller, L Zhao, HU Voss, SM Paul, C Schaffer, L Park, C Iadecola Apo?4 disrupts neurovascular regulation and undermines white matter integrity and cognitive function Nat Commun, 2018;9(1):3816. 2018 [PMID: 30232327] (ICC, Mouse) ICC Mouse
Show All 16 Publications.

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Biotin BAF2335

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Bioinformatics

Gene Symbol ANPEP
Uniprot