Aminopeptidase N/CD13 Antibody [Unconjugated]

Images

 
Aminopeptidase N/CD13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 15 µg/mL for 3 hours at room ...read more
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 0.1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Aminopeptidase N/CD13 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase N/CD13
Lys69-Ser966
Accession # P97449
Specificity
Detects mouse Aminopeptidase N/CD13 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANPEP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2335 in the following applications:

Publications
Read Publications using
AF2335 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody [Unconjugated]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

  1. Chen, H. et al. (1996) J. Immunol. 157:2593.
  2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
  3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
  4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aminopeptidase N/CD13 Antibody (AF2335)(32)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-F, IHC-Fr, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IHC
(24)
IHC-F
(2)
IHC-Fr
(4)
Western Blot
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(30)
Rat
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using AF2335 Applications Species
Jovanovic Macura, I;Zivanovic, A;Perovic, M;Ciric, J;Major, T;Kanazir, S;Ivkovic, S; The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer's Disease International journal of molecular sciences 2023-09-14 [PMID: 37762391] (IHC, Mouse) IHC Mouse
Bennett, HC;Zhang, Q;Wu, YT;Chon, U;Pi, HJ;Drew, PJ;Kim, Y; Aging drives cerebrovascular network remodeling and functional changes in the mouse brain bioRxiv : the preprint server for biology 2023-05-24 [PMID: 37305850] (IHC, Transgenic Mouse) IHC Transgenic Mouse
A Drieu, S Du, SE Storck, J Rustenhove, Z Papadopoul, T Dykstra, F Zhong, K Kim, S Blackburn, T Mamuladze, O Harari, CM Karch, RJ Bateman, R Perrin, M Farlow, J Chhatwal, Dominantly, S Hu, GJ Randolph, I Smirnov, J Kipnis Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid Nature, 2022-11-09;611(7936):585-593. 2022-11-09 [PMID: 36352225] (IHC, Mouse) IHC Mouse
MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus Elife, 2022-11-07;11(0):. 2022-11-07 [PMID: 36341713] (IHC, Mouse) IHC Mouse
S Kiani Shab, S Morabito, EP Danhash, A McQuade, JR Sanchez, E Miyoshi, JP Chadarevia, C Claes, MA Coburn, J Hasselmann, J Hidalgo, KN Tran, AC Martini, W Chang Roth, J Pascual, E Head, DA Hume, C Pridans, H Davtyan, V Swarup, M Blurton-Jo Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice Cell Reports, 2022-06-14;39(11):110961. 2022-06-14 [PMID: 35705056] (IHC, Mouse) IHC Mouse
JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022-05-25;0(0):. 2022-05-25 [PMID: 35614216] (IHC, Mouse) IHC Mouse
Q Zhao, W Dai, HY Chen, RE Jacobs, BV Zlokovic, BT Lund, A Montagne, A Bonnin Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation Proceedings of the National Academy of Sciences of the United States of America, 2022-04-04;119(15):e2113310119. 2022-04-04 [PMID: 35377817] (IHC-F, Mouse) IHC-F Mouse
YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (IHC, Mouse) IHC Mouse
T Higa, Y Okita, A Matsumoto, S Nakayama, T Oka, O Sugahara, D Koga, S Takeishi, H Nakatsumi, N Hosen, S Robine, MM Taketo, T Sato, KI Nakayama Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium Nature Communications, 2022-03-21;13(1):1500. 2022-03-21 [PMID: 35314700] (Flow Cytometry, IHC-F, Mouse) Flow Cytometry, IHC-F Mouse
TA Sakaue, Y Fujishima, Y Fukushima, Y Tsugawa-Sh, S Fukuda, S Kita, H Nishizawa, B Ranscht, K Nishida, N Maeda, I Shimomura Adiponectin accumulation in the retinal vascular endothelium and its possible role in preventing early diabetic microvascular damage Scientific Reports, 2022-03-09;12(1):4159. 2022-03-09 [PMID: 35264685] (Western Blot, Mouse) Western Blot Mouse
Show All 32 Publications.

Review for Aminopeptidase N/CD13 Antibody (AF2335) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2335:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Aminopeptidase N/CD13 AF2335
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 07/25/2022
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotVRR0322031

Comments

CommentsMouse brain tissue (12 micrometer thickness) was incubated overnight with AF2335, 1:500, in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody AlexaFluor 568-conjugated, 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. AF2335 signal identifies brain pericytes surrounding blood vessels.

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Verified Customer
07/25/2022
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ANPEP
Uniprot