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MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox" target="_blank">
HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
1310-SE) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human uPAR His-tag Avi-tag (Catalog # AVI807) binds with an ED50 of 40-240 ng/mL." class="big_lightbox" target="_blank">
uPAR (Urokinase plasminogen activator receptor) is a glycoprotein I anchored surface receptor specific for urokinase plasminogen activator (uPA). Upon binding to uPAR, uPA converts the surface bound, large serum beta globulin plasminogen to plasmin. Plasmin, which is also designated fibrinolysin, is a trypsin-like enzyme that acts on Arg-Lys bonds and induces pericellular proteolysis in fibrin and fibrinogen, and thereby contributes to the systematic activation of the coagulation cascade. uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells and are required for tumor invasion and metastasis.