Integrin beta 2/CD18 Antibody [Unconjugated]

Images

 
Integrin  beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin  beta 2/CD18 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Integrin beta 2/CD18 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2
Gln23-Asn700
Accession # AAA59490
Specificity
Detects human Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, recombinant mouse Integrin beta 2, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 5, rhIntegrin beta 6, and rhIntegrin beta 7 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGB2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Adhesion Blockade
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1730 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 2/CD18 Antibody [Unconjugated]

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

796-IC
Species: Mu
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
7268-CT
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF728
Species: Hu
Applications: WB, AdBlk
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-97871
Species: Mu
Applications: Flow, IHC, IHC-P, IP, CyTOF-ready
NBP2-25200
Species: Hu
Applications: WB, Flow, IHC, IHC-Fr, B/N
NBP2-22542
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
BBA16
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-64843
Species: Mu
Applications: Flow, IHC, IHC-Fr, IHC-P

Publications for Integrin beta 2/CD18 Antibody (AF1730)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Cell Migration, ICC, Neut, WB.


Filter By Application
Cell Migration
(1)
ICC
(1)
Neut
(3)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1730 Applications Species
BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities J. Immunol., 2018;0(0):. 2018 [PMID: 29686052] (Neut,, Human) Neut Human
X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu Bystander cells enhance NK cytotoxic efficiency by reducing search time Sci Rep, 2017;7(0):44357. 2017 [PMID: 28287155] (Cell Migration,, Human) Cell Migration Human
Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L, Jansen T, van de Veerdonk F, Williams D, Joosten L, Dinarello C, Netea M An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism. Cytokine, 2015;71(2):215-22. 2015 [PMID: 25461401] (Neut,, Human) Neut Human
Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Cell Commun Signal, 2014;12(0):36. 2014 [PMID: 24965524] (Neut,, Human) Neut Human
Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation. J. Biol. Chem., 2011;286(9):7070-81. 2011 [PMID: 21193407] (WB,, Human) WB Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007;52(10):2775-83. 2007 [PMID: 17394066] (ICC,, Human) ICC Human

Reviews for Integrin beta 2/CD18 Antibody (AF1730) (0)

There are no reviews for Integrin beta 2/CD18 Antibody (AF1730). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin beta 2/CD18 Antibody (AF1730) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Integrin beta 2/CD18 Antibody (AF1730)

Discover related pathways, diseases and genes to Integrin beta 2/CD18 Antibody (AF1730). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Integrin beta 2/CD18 Antibody (AF1730)

Discover more about diseases related to Integrin beta 2/CD18 Antibody (AF1730).
 

Pathways for Integrin beta 2/CD18 Antibody (AF1730)

View related products by pathway.

PTMs for Integrin beta 2/CD18 Antibody (AF1730)

Learn more about PTMs related to Integrin beta 2/CD18 Antibody (AF1730).

Blogs on Integrin beta 2/CD18.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Integrin beta 2/CD18 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol ITGB2
Entrez
Uniprot