Integrin beta 2/CD18 Antibody [Unconjugated]

Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 2/CD18 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Integrin beta 2
  Gln23-Asn700
  Accession # AAA59490
• Specificity: Detects human Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, recombinant mouse Integrin beta 2, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 5, rhIntegrin beta 6, and rhIntegrin beta 7 is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB2
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Adhesion Blockade
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 2/CD18 Antibody [Unconjugated]

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 2/CD18 Antibody (AF1730)(9)

Publications using AF1730

• LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity MBio, 2019;10(4):. 2019 [PMID: 31289186] (Western Blot, Human)
• MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis Sci Adv, 2019;5(3):eaau7518. 2019 [PMID: 30906859] (ICC, Mouse)
• BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
• SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities J. Immunol., 2018;0(0):. 2018 [PMID: 29686052] (Neutralization, Human)
• X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu Bystander cells enhance NK cytotoxic efficiency by reducing search time Sci Rep, 2017;7(0):44357. 2017 [PMID: 28287155] (Migration Assay, Human)
• Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L, Jansen T, van de Veerdonk F, Williams D, Joosten L, Dinarello C, Netea M An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism. Cytokine, 2015;71(2):215-22. 2015 [PMID: 25461401] (Neutralization, Human)
• Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Cell Commun Signal, 2014;12(0):36. 2014 [PMID: 24965524] (Neutralization, Human)
• Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation. J. Biol. Chem., 2011;286(9):7070-81. 2011 [PMID: 21193407] (Western Blot, Human)
• van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007;52(10):2775-83. 2007 [PMID: 17394066] (ICC, Human)

Bioinformatics

• Gene Symbol: ITGB2
• Uniprot:
  • AAA59490()