Integrin alpha L/CD11a Antibody (345913)

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin alpha L/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha L/CD11a at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 345913
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha L/CD11a Antibody (345913) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha L
  Tyr26-Met1089 (Tyr660Ile)
  Accession # CAA68747
• Specificity: Detects human Integrin alpha L in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAL
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha L/CD11a Antibody (345913)

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Integrin alpha L/CD11a Antibody (MAB3595) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20810987
• Species: Human

Blocking

• Blocking Details: See PMID 20810987

Details

• Detection Notes: See PMID 20810987

Comments

• Comments: Independent review from antibodyresource.com

Bioinformatics

• Gene Symbol: ITGAL
• Uniprot:
  • CAA68747()