Lymphotoxin beta R/TNFRSF3 Antibody

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lymphotoxin beta R/TNFRSF3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
  Ser28-Met227
  Accession # P36941
• Specificity: Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LTBR
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Inhibition of Cell Growth
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin beta R/TNFRSF3 Antibody

• CD18
• D12S370
• ltbetar
• LT-BETA-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp), was originally identified as a transcribed sequence on human chromosome 12p with homology to the TNF receptor superfamily. In the new TNF nomenclature, LT beta R is referred to as TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motif characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R share little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be utilized. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. The human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
2. Rennert, P.D. et al. (1998) Immunity 9:71.
3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
5. Crowe, P.D. et al. (1994) Science 264:707.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta R/TNFRSF3 Antibody (AF629)(10)

Publications using AF629

• M Maksymowic, M Mi?czy?ska, M Banach-Or? Clathrin- and dynamin-dependent endocytosis limits canonical NF-&amp;kappaB signaling triggered by lymphotoxin &amp;beta receptor Cell Commun Signal, 2020-11-04;18(1):176. 2020-11-04 [PMID: 33148272] (Western Blot, Human)
• Siyuan Ding, Yanhua Song, Kevin F. Brulois, Junliang Pan, Julia Y. Co, Lili Ren et al. Retinoic Acid and Lymphotoxin Signaling Promote Differentiation of Human Intestinal M Cells Gastroenterology 7/1/2020 [PMID: 32247021]
• Magdalena Banach-Orłowska, Renata Wyszyńska, Beata Pyrzyńska, Małgorzata Maksymowicz, Jakub Gołąb, Marta Miączyńska Cholesterol restricts lymphotoxin beta receptor-triggered NF-kappa B signaling Cell Communication and Signaling 12/26/2019 [PMID: 31878945]
• M Banach-Or?, K Jastrz?bsk, J Cendrowski, M Maksymowic, K Wojciechow, M Korosty?sk, D Moreau, J Gruenberg, M Miaczynska The topology of lymphotoxin ? receptor accumulated upon endolysosomal dysfunction dictates the NF-?B signaling outcome J. Cell. Sci., 2018-11-21;0(0):. 11/21/2018 [PMID: 30333141]
• Yinghui Li, Qi-Ling Zhou, Wenjie Sun, Prashant Chandrasekharan, Hui Shan Cheng, Zhe Ying et al. Non-canonical NF-kappa B signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation Nature Cell Biology 10/1/2015 [PMID: 26389665]
• Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L TNF-alpha blockade induces IL-10 expression in human CD4+ T cells. Nat Commun, 2014-01-01;5(0):3199. 2014-01-01 [PMID: 24492460] (Neutralization, Human)
• Corinne Ganeff, Caroline Remouchamps, Layla Boutaffala, Cécile Benezech, Géraldine Galopin, Sarah Vandepaer et al. Induction of the Alternative NF-kappa B Pathway by Lymphotoxin alpha beta (LT alpha beta ) Relies on Internalization of LT beta Receptor Molecular and Cellular Biology 11/1/2011 [PMID: 21896778]
• Takahashi A, Ohtani N, Yamakoshi K, Iida S, Tahara H, Nakayama K, Nakayama KI, Ide T, Saya H, Hara E Mitogenic signalling and the p16INK4a-Rb pathway cooperate to enforce irreversible cellular senescence. Nat. Cell Biol., 2006-10-08;8(11):1291-7. 2006-10-08 [PMID: 17028578] (Western Blot, Human)
• Girard S, Vossman E, Misek DE, Podevin P, Hanash S, Brechot C, Beretta L Hepatitis C virus NS5A-regulated gene expression and signaling revealed via microarray and comparative promoter analyses. Hepatology, 2004-09-01;40(3):708-18. 2004-09-01 [PMID: 15349911] (Western Blot, Human)
• Hikichi Y, Tsuji I, Shintani Y LIGHT, a member of the tumor necrosis factor ligand superfamily, prevents tumor necrosis factor-alpha-mediated human primary hepatocyte apoptosis, but not Fas-mediated apoptosis. J. Biol. Chem., 2002-10-18;277(51):50054-61. 2002-10-18 [PMID: 12393901] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: LTBR
• Uniprot:
  • P36941()