CD8 Products

Antibodies
CD8 Antibody (YTS105.18) - BS ...
CD8 Antibody (YTS105.18) - BSA Free
NB200-578
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P (-)
Host: Rat Monoclonal
Antibody Pairs
CD8 Antibody Pair [HRP]
CD8 Antibody Pair [HRP]
NBP2-79328
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA, ICC/IF
Host: Mouse Monoclonal
Conjugate Catalog # Availability Size Price
CD8 Antibody Pair [HRP]
CD8 Antibody Pair [HRP]
NBP2-79574
Species: Mu
Applications: IP, S-ELISA, WB (-)
Host: Rabbit
Conjugate Catalog # Availability Size Price
CD8 Antibody Pair [HRP]
CD8 Antibody Pair [HRP]
NBP2-79612
Species: Ma
Applications: ELISA, S-ELISA
Host: Monoclonal
Conjugate Catalog # Availability Size Price
ELISA Kits
Human CD8 alpha DuoSet ELISA, ...
Human CD8 alpha DuoSet ELISA, 5 Plate
DY1509-05
Species: Hu
Applications: ELISA
Rat CD8 ELISA Kit (Colorimetr ...
Rat CD8 ELISA Kit (Colorimetric)
NBP2-75162
Species: Rt
Applications: ELISA
Mouse CD8 ELISA Kit (Colorime ...
Mouse CD8 ELISA Kit (Colorimetric)
NBP2-78729
Species: Mu
Applications: ELISA
Lysates
CD8 Overexpression Lysate
CD8 Overexpression Lysate
NBP2-10773
Species: Hu
Applications: WB
CD8 Overexpression Lysate
CD8 Overexpression Lysate
NBL1-08980
Species: Hu
Applications: WB
CD8 Overexpression Lysate
CD8 Overexpression Lysate
NBP2-11336
Species: Hu
Applications: WB
Proteins
Recombinant Human CD8 alpha F ...
Recombinant Human CD8 alpha Fc Chi...
AVI10927
Species: Hu
Applications: Bioactivity
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  
Recombinant Mouse CD8 alpha/b ...
Recombinant Mouse CD8 alpha/beta H...
11146-CD
Species: Mu
Applications: Bioactivity
Formulation Catalog # Availability Price  
Recombinant Human CD8 alpha F ...
Recombinant Human CD8 alpha Fc Chi...
10927-CD
Species: Hu
Applications: Bioactivity
Formulation Catalog # Availability Price  

Description

CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.

References

1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208

Bioinformatics

Product By Gene ID 925
Alternate Names
  • CD_antigen: CD8a
  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a molecule
  • CD8A
  • Leu2 T-lymphocyte antigen
  • LEU2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

PTMs for CD8

Learn more about PTMs related to CD8.

Demethylation
Methylation

Bioinformatics Tool for CD8

Discover related pathways, diseases and genes to CD8. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...    Read more.
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By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...    Read more.
Synthetic Biotic Medicine as Immunotherapy Against Cancer: Evidence From Arginine-Producing Engineered Bacteria
By Jamshed Arslan, Pharm D, PhDWhat do nuts, dairy and red meat have in common? In addition to the fact that they are all edible, one of the answers is L-arginine. This amino acid improves T cell’s respons...    Read more.
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