Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - Human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - Renal carcinoma tissue stained with polyclonal carbonic anhydrase IX, NB 100-417.
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock ...read more
This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24700751) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, dog, rat and mouse (PMID 22842475). Plant reactivity reported in scientific literature (PMID: 19278636)
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Carbonic Anhydrase IX/CA9 Antibody [Biotin]
CAIXcarbonic anhydrase 9
Carbonate dehydratase IX
Carbonic Anhydrase IX
carbonic anhydrase IXpMW1
Membrane antigen MN
MNRCC-associated antigen G250
RCC-associated protein G250
Renal cell carcinoma-associated antigen G250
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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