Carbonic Anhydrase IX/CA9 Antibody [Biotin]

Images

 
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - Human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - Renal carcinoma tissue stained with polyclonal carbonic anhydrase IX, NB 100-417.
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [Biotin] [NB100-417B] - HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PlSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Carbonic Anhydrase IX/CA9 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]
Localization
Membrane.
Marker
Hypoxia Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CA9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24700751) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Positive Control
Carbonic Anhydrase IX/CA9 Lysate (NBL1-08619)
Publications
Read Publications using
NB100-417B in the following applications:

Reactivity Notes

Human, dog, rat and mouse (PMID 22842475). Plant reactivity reported in scientific literature (PMID: 19278636)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody [Biotin]

  • CA9
  • CA-IX
  • CAIXcarbonic anhydrase 9
  • Carbonate dehydratase IX
  • Carbonic Anhydrase IX
  • carbonic anhydrase IXpMW1
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • MNRCC-associated antigen G250
  • P54/58N
  • RCC
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (NB100-417B)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


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ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
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Human
(1)
Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Carbonic Anhydrase IX/CA9 Antibody (NB100-417B) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB100-417G
DyLight 550 Labeled NB100-417R
DyLight 650 Labeled NB100-417C
HRP Labeled NB100-417H

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Bioinformatics Tool for Carbonic Anhydrase IX/CA9 Antibody (NB100-417B)

Discover related pathways, diseases and genes to Carbonic Anhydrase IX/CA9 Antibody (NB100-417B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Carbonic Anhydrase IX/CA9.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

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Bioinformatics

Gene Symbol CA9
Entrez