Anti-Carbonic Anhydrase IX/CA9 Antibodies: Novus Biologicals

NB100-417

Carbonic Anhydrase IX/CA9 Antibody - BSA Free

(7 Reviews)

(294 Publications)

0.1 ml

NB100-417

<b>Orthogonal Strategies Validation. </b>Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for Carbonic Anhydrase IX/CA9 mRNA (ACD RNAScope Probe, catalog # 559348; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus catalog # NB100-417) at 1:1000 dilution with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Analysis on various human cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 in HEK-293 and A498 cell lines at a molecular weight of approximately 50 kDa. WB image submitted by a verified customer review.

Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-NB100-417-img0040.jpg

<b>Biological Strategies Validation. </b>Hypoxias impact on proteins of the mitochondrial ISC assembly machinery. (A) Immunoblotting analyzed the total protein extracts from HeLa cells grown in normoxia (Nx, 21% O2) or hypoxia (Hx, 1% O2) conditions using VDACs poly antibody and anti-CAIX, -ISCU, -FXN, -NFS1, -HSC20 antibodies. Beta-Actin was used as loading control. Citation: Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.

<b>Biological Strategies Validation. </b>Analysis using the DyLight 488 conjugate of NB100-417. Staining of Carbonic Anhydrase IX (red) in human glioma U87 cells. DAPI counterstains nuclei (blue). ICC/IF image submitted by a verified customer review.

Simple Western lane view shows a specific band for CAIX in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

An intracellular stain was performed on U-87 MG Cells with NB100-417 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody, (R&D Systems, F0111).

Western Blot analysis of Carbonic Anhydrase IX/CA9 Antibody on human breast cancer MCF7 cells. Image from verified customer review.

A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Carbonic Anhydrase IX/CA9 Antibody NB100-417 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 Antibody NB100-417 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Analysis in 1) HeLa, 2) MDA-MB-231, and 3) A549 whole cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 at a molecular weight of 50 kDa.

Analysis on rat renal cortex. A specific band was detected at a molecular weight of approximately 50 kDa.

Carbonic-Anhydrase-IX-CA9-Antibody-Immunocytochemistry-Immunofluorescence-NB100-417-img0039.jpg

Analysis of human colon carcinoma, xenografted in mice. IHC-Fr image submitted by a verified customer review.

IHC analysis of a FFPE tissue section of human breast cancer using CAIX antibody at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This CAIX antibody generated an expected cytoplasmic staining of CAIX protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed CAIX staining is specific.

Strong cytoplasmic and membranous CA9 staining in glandular cells of human stomach tissue. Citrate buffer pH 6 antigen retrieval. Antibody at 1:250 dilution and detected with an Alexa Fluor 546 labeled goat-anti-rabbit secondary antibody. Nuclei detected with DAPI. IHC-P image submitted by a verified customer review.

Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.

Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-NB100-417-img0046.jpg

<b>Genetic Strategies Validation. </b>Iron depletion and nitric oxide stress and Iron depletion induce the truncated VDAC1 form accumulation. (D) Total protein extracts were analyzed by immunoblotting using VDACs poly antibody and anti-HIF-1 alpha and -CAIX antibodies. (E) Immunoblotting was used to analyze total protein extracts using antibodies against the three VDAC isoforms. (F) HeLa cells were grown in hypoxia (Hx, 1% O2) conditions and transfected with iscu- or NC-siRNA for 3 days, or grown in normoxia (Nx, 21% O2), some treated with DFO for 16 h. Western Blots analyzed total proteins using VDACs poly antibody and anti-HIF-1 alpha, -CAIX, -ISCU antibodies. Citation: Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.

Immunohistochemical representative microphotographs representing the HIF-1 alpha , GLUT-1, and CAIX expression in endometrial cancer according to FIGO classification (IA, IB, II, IIIA, IIIC, and IV). Primary objective magnification 20x.

The images are representative of immunohistochemistry staining of nuclear staining of proliferation marker Ki67, membrane protein CD 44, and CA IX. The images of scaffold culture (left panel) are compared with cell pellet paraffin section (right panel). The scaffold dissolved in xylene presented as a clear region marked ∗. All scale bars are 100 μm, magnification 40x.

Chemosensitivity of the CTC SCLC lines (IC50, mean ± SD) and immunohistochemistry of sections of tumorospheres. All differences between single cells (SC) and tumorospheres (TS) are statistically significant. Immunohistochemical staining of sections of UHGc5, BHGc16 and BHGc26 CTCs was performed using antibodies directed to CD56, CHGA, CAIX and Ki67, respectively.

Western Blot: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Analysis of protein levels of genes with increased transcription in hypoxia, IOX2 & VH032.HIF targets were increased in hypoxia, VH298 & FG-4592. 0.05% DMSO (vehicle control), 1% O2 (hypoxia), 100 µM VH298 & 50 µM FG-4592 were introduced to (A) HeLa or HFF for 24 hours & (B) HeLa for indicated time. Protein levels were analysed by immunoblotting using antibodies against indicated proteins, with beta -Actin as loading control. The blots shown are representative of three independent experiments. * indicates longer exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30801039), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - IGF1R & CA9 expression in PDAC.(A) Representative staining of CA9 cells quantified on a scoring system of 0–3 according to staining intensity (original magnification ×200). CA9 was mainly expressed in the cell membrane of PDAC cells. (B) IGF1R was mainly expressed in the cytoplasm of PDAC cells. (C) Association between IGF1Rexpression & CA9 expression in 120 pancreas cancer. A significant correlation was found between IGF1R expression level was significantly correlated with CA9 expression in PDAC cells (p < 0.01, rs = 0.649; Spearman’s rank sum test). The size of circle indicates the number of PDAC patients. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27487118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Representative human colorectal cancer specimen. Immunohistochemistry of CA9 & GGT1 in mLNs. In these cases, both CA9 & GGT1 were expressed, suggesting that GGT1 expression is related to hypoxia in mLNs. Scale bar, 500 μm. Black arrowheads: GGT of cancer cells, white arrowheads: GGT surrounding cancer cells, arrow: accumulation of GGT. mLNs: metastatic lymph nodes, GGT: gamma-glutamyl transpeptidase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542087), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Representative microscopy images of staining for hypoxia markers in prostate tissues (MO, 400×). A) HIF-1 alpha - notice the granular cytoplasmic immunoreactivity of the malignant epithelial cells. In this case, more than 50% of the glands stained. B) LOX - strong & diffuse nuclear immunoreactivity of the epithelial cells. C) CAIX - note a focal apical cytoplasmic immunoreactivity in epithelial cells. D) VEGFR2 - moderate nuclear & weak cytoplasmic expression of the epithelial cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28143503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - The images are representative of immunohistochemistry staining of nuclear staining of proliferation marker Ki67, membrane protein CD 44, & CA IX. The images of scaffold culture (left panel) are compared with cell pellet paraffin section (right panel). The scaffold dissolved in xylene presented as a clear region marked ∗. All scale bars are 100 μm, magnification 40x. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24101930), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Hypoxia, blood vessel & proliferation staining. IHC staining in the Biopsy 23 & Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 & LYVE1. 15× Magnification. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - VH298 elicits a HIF-dependent hypoxic response in various cell lines.1% DMSO, hypoxia (1% O2) & indicated compounds at 100 μM were introduced to (a) HFF, HeLa, U2OS & (b) RCC4 cells expressing HA-tagged VHL (with the exception of cis-VH298 & VH298 used at 150 μM) for 24 h & (c) CTLs for 8 h. Protein levels were analysed by immunoblotting using antibodies against the indicated proteins, with beta -actin (SMC1 for CTLs) as the loading control. The blots shown are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27811928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - HE staining & immunohistochemistry (CA9 & GGT1) of fixed primary tumor & mLN specimens from orthotopic mouse model. CA9 & GGT1 were expressed inside the primary tumor & in mLNs. GGT1 was accumulated in the area of central necrosis. HE: hematoxylin-eosin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542087), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Comparison between primary tumor & mLNs of the mouse model. Expression of CA9 & GGT1 in mLNs was higher than that in the primary tumor at 1week after injection of cells. (A) CA9 & GGT1 expression of HT29 mLNs compared to primary tumor at 6 weeks or 1 week after injection of cells. (B) Tissue oxygen concentration (%) of rectal submucosa (SM) & nLNs (n = 3). Error bars represent SD. mNs: metastatic lymph nodes, nLNs: non metastatic lymph nodes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542087), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Chemosensitivity of the CTC SCLC lines (IC50, mean ± SD) & immunohistochemistry of sections of tumorospheres. All differences between single cells (SC) & tumorospheres (TS) are statistically significant. Immunohistochemical staining of sections of UHGc5, BHGc16 & BHGc26 CTCs was performed using antibodies directed to CD56, CHGA, CAIX & Ki67, respectively. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05562-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Immunocytochemical analysis of CNHCs with antibodies against the RCC marker CAIX. Clusters of CNHCs cytomorphologically classified as uncertain malignant (−UMF) with cytoplasmic positive staining with antibodies against the RCC marker CAIX (A). Clusters of CNHC-UMF & -BF without reactivity for CAIX antibodies (B & C, respectively). A single CNHC-MF with positive cytoplasmic (D) & without staining for CAIX (E). Single CAIX-negative CNHC-UMF & -BF (F & G, respectively). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - GPRC5A promotes hypoxic cell survival via YAPAHypoxia induced increased total YAP expression in a panel of colorectal cancer cell lines. Actin confirmed equal loading; blots are representative of at least two independent experiments. Related to Fig 4.B–EqRT–PCR analysis revealed that hypoxia increased the expression of known YAP target genes AREG, BCL2L1 (BCL‐XL), CTGF & CYR61. Representative experiments are shown. Representative examples of n = 3 independent experiments are shown; data are presented as mean ± SD.FIncreased expression of YAP Ser397 in GPRC5A‐depleted cells was overridden by expression of constitutively active RhoA (G14V) in hypoxia. Expression of dominant negative RhoA (T19N) did not further increase YAP Ser397 phosphorylation in response to GPRC5A depletion.GDarker exposure of the GPRC5A blot in Fig 4I, confirming expression of 30–40 kDa & 80 kDa species. Note that YAP siRNA partially diminishes GPRC5A in line with the existence of positive HIF‐GPRC5A‐YAP feedback loop. Asterisk (*) indicates non‐specific band.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - CAIX expression in PDAC & association with outcomeA. Representative images of immunohistochemical staining showing: negative expression of CAIX in both tumor cell & stromal compartments (top left), positive expression of CAIX in tumor cells but not stromal compartment (top right), positive expression of CAIX in stromal compartment but not tumor cells (bottom left), positive expression of CAIX in both tumor cell & stromal compartments (bottom right). B. Kaplan-Meier analysis of CAIX expression in the distinct tumor cell & stromal compartments & the associated overall survival. C. Representative images of immunohistochemical staining showing low stromal volume & high stromal volume & low & high microvessel density D. Representative images of immunohistochemical staining showing: positive expression of MCT4 in tumor cell compartment & positive expression of MCT4 in tumor stromal compartment. Kaplan-Meier analysis of combinatorial MCT4 & CAIX expression in the stromal compartment & the associated overall survival. Hazard ratios & p-values for each comparison are summarized in the table. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Hypoxia & autophagy markers in breast cancer stem-cells(A) Co-expression of CD133 & BECLIN1 in pCR & non-pCR patients. Double IF X800. (B) Tumor cells (T) distributed around necrosis (N) express LC3B with typical cytoplasmic punctuated staining, ×1000. Double IF shows that some CD133-positive tumor cells co-express LC3B. ×400. (C) Tumor cells (T) distributed around necrosis (N) express CAIX, on their cytoplasmic membrane, ×1000. Double IF shows a co-expression of LC3B & CAIX in some tumor cells. ×400. (D) Laser microdissection of CD133-positive cells. (E) Laser-microdissected CD133-positive cells express PROMININ & not PTPRC (CD45). (F) mRNA relative quantification of HIF1A, CAIX, LC3B, BECN1, & BNIP3L show a significantly higher expression level in CD133-positive cells versus CD133-negative cells, in the 35 non-pCR biopsies. *p < 0.05. (G) Multivariate analysis of tumor cell characteristics & pCR shows an inverse correlation between pCR & cells bearing markers of stemness or autophagy. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28445132), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Immunofluorescent images showing CAIX expression, pimonidazole, apoptosis & proliferation in SCCNij202.CAIX is expressed in hypoxic regions as assessed by pimonidazole staining (A), but is also observed in non-pimonidazole areas (B). Yet, most of CAIX is expressed in pimonidazole positive regions (C). Proliferation (BrdUrd) & apoptosis (caspase-3) in relation to CAIX expression are shown in figure D & E respectively. Red, CAIX; Green, pimonidazole (A–B), BrdUrd (D) or caspase-3 (E); Yellow, overlap of CAIX (red) & pimonidazole (green); Light blue, vessels. Magnification 100×. Scale bars represent 100 µm. Closed circles represent CAIX expression in pimonidazole positive regions; open circles represent CAIX expression in pimonidazole negative regions. Abbrevations: 1× S4, 8 h, one i.p. injection S4, harvest after 8 hours; 1× S4, 24 h, one i.p. injection S4, harvest after 24 hours; 3× S4, one i.p. injection S4 a day for 3 days, harvest 8 hours after the last injection; 5× S4, one i.p. injection S4 a day for 5 days, harvest 8 hours after the last injection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25225880), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Colorectal cancer xenograft growth was suppressed by CB-PIC(20 & 50 mg/kg body weight) in female athymic nude mice. Starting three days after SW620 cell inoculation, CB-PIC (20 & 50 mg/kg body weight) was injected in abdomen with 4% Tween 20 as vehicle once daily. (a) Body weights of mice. (b) Tumor growth in a time course. (c) Final tumor weight at termination of experiment. (d) Representative examples of immunohistochemical staining for Ki-67, CD34, TUNEL, CA IX & pAMPK alpha in tumor sections. Graphs show the Ki67 index (proliferation), CD34 index (angiogenesis), TUNEL index (apoptosis), CA IX (hypoxia region), & pAMPK alpha index in tumor sections. Values are means ± SD, n = 6. *P < 0.05 & ***P < 0.001 compared with control mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23589723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Hypoxia induces GPRC5A directly via HIFsASILAC‐based proteomics data identify known (red) & novel (green) hypoxia‐induced proteins in SW620 cells. One‐sample t‐test was performed.BWestern blotting confirmed GPRC5A as a hypoxia‐induced protein in SILAC lysates.CValidation of GPRC5A Western blot data using siRNA. *Non‐specific band of ˜60 kDa not depleted by GPRC5A siRNA.DConfocal microscopy showing plasma membrane GPRC5A expression in hypoxic SW620 cells (scale bars: 75 μm).EWestern blotting showing GPRC5A upregulation by hypoxia in a panel of colorectal tumour cell lines.FBasal & hypoxia‐induced GPRC5A protein expression was decreased by HIF‐1/2 alpha depletion.GDepletion of HIF‐1 beta decreased GPRC5A protein upregulation in hypoxia.HHypoxia mimetic DMOG induced HIF‐1/2 alpha , CA9 & GPRC5A protein expression. Dual HIF‐1/2 alpha depletion reduced GPRC5A induction by DMOG.IqRT–PCR demonstrating that GPRC5A mRNA was upregulated by hypoxia (n = 3). GPRC5A was normalised to HPRT (error bars ± SD).JqRT–PCR demonstrating that HIF‐1/2 alpha depletion decreased GPRC5A induction during hypoxia (n = 3). GPRC5A was normalised to HPRT (error bars ± SD).KChIP‐PCR analyses identify HIF‐1 alpha binding to the GPRC5A promoter region containing a putative optimal HRE (error bars ± SD, n = 3).Data information: Asterisks (*) indicate non‐specific band. Level adjustments were made to images in Adobe Photoshop post‐acquisition for clarity (equal changes applied to the entire image). Representative examples of n = 3 independent experiments are shown.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Carbonic Anhydrase IX/CA9 Antibody - BSA Free [NB100-417] - Establishment of a human protein replacement stable cell line using 47snoMEN.(a) Images of protein replacement stable cell lines (U2OSGFP–HIF-1 alpha -47PR). Expression of FP proteins was confirmed by fluorescence imaging with DFX treatment. Bar is 10 μm. (b) U2OS & U2OSGFP–HIF-1 alpha -47PR cells were exposed to 1% O2 for the indicated periods of time prior to lysis. Cell extracts were analysed by western blot using the indicated antibodies. (c) U2OS cells were transfected with 1 μg of control & HIF-1 alpha -47snoMEN vectors for 48 hours prior to lysis. DFX was added for the last 24 hours. Cell extracts were analysed by western blot using the indicated antibodies. (d) 3D culture analysis. HeLaGFP–HIF-1 alpha -47PR cells were cultured in lipidure coated plates to induce spheroid formation. HIF-1 alpha is visible in the core of the spheroid revealing hypoxia. Bar is 10 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154759), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pl

Species Glossary

Reactivity: Hu, Mu, Rt, Pl

Applications:

, , , , , , , , , , , ,

Applications: , , , , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-417AF350
Alexa Fluor 405	NB100-417AF405
Alexa Fluor 488	NB100-417AF488
Alexa Fluor 532	NB100-417AF532
Alexa Fluor 594	NB100-417AF594
Alexa Fluor 647	NB100-417AF647
Alexa Fluor 700	NB100-417AF700
Alexa Fluor 750	NB100-417AF750
Alexa Fluor Plus 405	NB100-417AFP405
Alexa Fluor Plus 488	NB100-417AFP488
Alexa Fluor Plus 555	NB100-417AFP555
Alexa Fluor Plus 594	NB100-417AFP594
Alexa Fluor Plus 647	NB100-417AFP647
Alexa Fluor Plus 680	NB100-417AFP680
Allophycocyanin	NB100-417APC
Allophycocyanin/Cy7	NB100-417APCCY7
Biotin	NB100-417B
CoraFluor 1	NB100-417CL1
DyLight 350	NB100-417UV
DyLight 405	NB100-417V
DyLight 488	NB100-417G
DyLight 550	NB100-417R
DyLight 594	NB100-417DL594
DyLight 650	NB100-417C
DyLight 680	NB100-417FR
DyLight 755	NB100-417IR
FITC	NB100-417F
HRP	NB100-417H
Janelia Fluor 525	NB100-417JF525
Janelia Fluor 549	NB100-417JF549
Janelia Fluor 646	NB100-417JF646
Janelia Fluor 669	NB100-417JF669
PE	NB100-417PE
PE/Atto594	NB100-417PEATT594
PE/Cy5.5	NB100-417PECY55
PE/Cy7	NB100-417PECY7
PerCP	NB100-417PCP
mFluor Violet 450 SE	NB100-417MFV450
mFluor Violet 500 SE	NB100-417MFV500
mFluor Violet 610 SE	NB100-417MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-417SS

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NBP1-51691

Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

(2 Reviews)

(14 Publications)

0.1 ml

NBP1-51691

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Carbonic Anhydrase IX/CA9 Antibody [2D3] (NBP1-51691) at 2ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Mouse choroid stained with anti-Carbonic Anhydrase IX antibody. ICC/IF image submitted by a verified customer review.

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-51691AF350
Alexa Fluor 405	NBP1-51691AF405
Alexa Fluor 488	NBP1-51691AF488
Alexa Fluor 532	NBP1-51691AF532
Alexa Fluor 594	NBP1-51691AF594
Alexa Fluor 647	NBP1-51691AF647
Alexa Fluor 700	NBP1-51691AF700
Alexa Fluor 750	NBP1-51691AF750
Alexa Fluor Plus 405	NBP1-51691AFP405
Alexa Fluor Plus 488	NBP1-51691AFP488
Alexa Fluor Plus 555	NBP1-51691AFP555
Alexa Fluor Plus 594	NBP1-51691AFP594
Alexa Fluor Plus 647	NBP1-51691AFP647
Alexa Fluor Plus 680	NBP1-51691AFP680
Allophycocyanin	NBP1-51691APC
Allophycocyanin/Cy7	NBP1-51691APCCY7
Biotin	NBP1-51691B
CoraFluor 1	NBP1-51691CL1
DyLight 350	NBP1-51691UV
DyLight 405	NBP1-51691V
DyLight 488	NBP1-51691G
DyLight 550	NBP1-51691R
DyLight 594	NBP1-51691DL594
DyLight 650	NBP1-51691C
DyLight 680	NBP1-51691FR
DyLight 755	NBP1-51691IR
FITC	NBP1-51691F
HRP	NBP1-51691H
Janelia Fluor 525	NBP1-51691JF525
Janelia Fluor 549	NBP1-51691JF549
Janelia Fluor 646	NBP1-51691JF646
Janelia Fluor 669	NBP1-51691JF669
PE	NBP1-51691PE
PE/Atto594	NBP1-51691PEATT594
PE/Cy5.5	NBP1-51691PECY55
PE/Cy7	NBP1-51691PECY7
PerCP	NBP1-51691PCP
mFluor Violet 450 SE	NBP1-51691MFV450
mFluor Violet 500 SE	NBP1-51691MFV500
mFluor Violet 610 SE	NBP1-51691MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-51691SS
Azide and BSA Free	NBP2-80611

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AF2188

Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

(1 Review)

(18 Publications)

100 μg

AF2188

HAF109). A specific band was detected for Carbonic Anhydrase IX/CA9 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of U-87 MG human glioblastoma/ astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). A specific band was detected for Carbonic Anhydrase IX/CA9 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of U-87 MG human glioblastoma/ astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Carbonic Anhydrase IX/CA9 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Carbonic Anhydrase IX/CA9 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

$Beta-catenin enhances the breast cancer stem cell phenotype in response to hypoxia independently of its nuclear transcriptional activity. A, Western analysis (WB) of beta-catenin, SNAI2 and CA9 protein levels in Ctrl/shBeta MCF7 cells upon Nor/1%pO2 conditions; B, MS forming assay in stable beta-catenin silenced (shBeta) MCF7 cells upon Nor/1%pO2 conditions; C, Cytofluorimetric analysis of CD44high/CD24low stem/progenitor population in ctrl/shBeta MCF7 cells upon Nor/1%pO2 conditions; D, Real-Time PCR analysis of ESR1 mRNA level in Ctrl/shBeta MCF7 cells upon Nor/1%pO2 conditions; E, Immunofluorescence (IF) analysis of Beta-catenin in Nor/1%pO2 MCF7 cells; F, WB analysis of beta-catenin in Nor/1%pO2 MCF7 cells cytoplasmic and nuclear fractions (lamin B and beta-tubulin were used as fractionation controls); G, beta-catenin/TCF transcriptional reporter (TOPFLASH) assay in MCF7 cells and T-MS under Nor/1%pO2. Data are presented as mean +/- s.d.; p values refers to t test. n=3, unless otherwise specified. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24260469), licensed under a CC-BY license. Not internally tested by R&D Systems.$

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

U87-MG cells were stained with Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

In normoxic basal-like breast cancer cells, cytoplasmic beta-catenin promotes stem cell features in vitro and tumor growth in vivo via constitutive stabilization of CA9 and SNAI2 mRNAs.A, Beta-catenin IF analysis in luminal MCF7 cells and in basal-like MDA-MB-468 and MDA-MB-231 cells; B, Cytofluorimetric analysis of the CD44high/CD24low population in ctrl/shBeta MDA-MB-468 and MDA-MB-231 cells; C, Ctrl/shBeta MDA-MB-468 10-weeks mammary fat pad xenograft assay (n=5, each group); representative pictures of xenograft tissues hematoxylin-eosin staining are include; D, WB analysis of CA9, SNAI2 and beta-catenin protein levels in ctrl/shBeta normoxic MDA-MB-468 and MDA-MB-231 cells; E, CA9 and SNAI2 mRNA stability assay following inhibition of Polymerase 2 transcriptional activity by actinomycin D (100ng/ml) in Ctrl/shBeta MDA-MB-468 and MDA-MB-231 cells; F, Four weeks growth curve of ctrl/shSNAI2 MDA-MB-231 subcutaneous xenograft assay (n=6, each group); G, Hematoxylin-eosin, ESR1 and CDH1 immunohistochemical stainings in xenograft tissue sections. Data are presented as mean +/- s.d.; p values refers to t test. n=3, unless otherwise specified. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24260469), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sunitinib induces hypoxia and Carbonic Anhydrase IX (CAIX) expression in primary Triple Negative Breast Cancer (TNBC) tumors. (a) Images of MDA-MB-231 LM2-4Luc+ primary tumor tissue sections harvested at increasing tumor volumes from animals administered either vehicle or 60 mg/kg sunitinib and immunohistochemically stained for the indicated markers. Scale bars: upper panels, 1 mm; lower panels, 200 μm. (b to e) Image-based quantification of (b) CD31+ blood vessels, (c) CAIX expression, (d) pimonidazole and (e) proliferation. Data show the mean ± standard error of the mean (SEM). n = 3 animals/group with 10 images/animal. * p < 0.05, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31319613), licensed under a CC-BY license. Not internally tested by R&D Systems.

2188-CA) was serially diluted and captured by Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Carbonic Anhydrase IX/CA9 (Catalog # <a class=

2188-CA) was serially diluted and captured by Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2188U-100UG
Alexa Fluor 405	AF2188V-100UG
Alexa Fluor 488	AF2188G-100UG
Alexa Fluor 532	AF2188X-100UG
Alexa Fluor 594	AF2188T-100UG
Alexa Fluor 647	AF2188R-100UG
Alexa Fluor 700	AF2188N-100UG
Alexa Fluor 750	AF2188S-100UG
Alexa Fluor Plus 405	AF2188AFP405-100UG
Alexa Fluor Plus 488	AF2188AFP488-100UG
Alexa Fluor Plus 555	AF2188AFP555-100UG
Alexa Fluor Plus 594	AF2188AFP594-100UG
Alexa Fluor Plus 647	AF2188AFP647-100UG
Alexa Fluor Plus 680	AF2188AFP680-100UG
Allophycocyanin	AF2188A
Biotin	BAF2188
CoraFluor 1	AF2188CL1
DyLight 350	AF2188D
DyLight 405	AF2188E
DyLight 488	AF2188K
DyLight 550	AF2188L
DyLight 594	AF2188M
DyLight 650	AF2188W
DyLight 680	AF2188Y
DyLight 755	AF2188Z
FITC	AF2188F
HRP	AF2188H
Janelia Fluor 525	AF2188JF525
Janelia Fluor 549	AF2188I
Janelia Fluor 646	AF2188J
Janelia Fluor 669	AF2188JF669
PE	AF2188P
PerCP	AF2188C
mFluor Violet 450 SE	AF2188MFV450
mFluor Violet 500 SE	AF2188MFV500
mFluor Violet 610 SE	AF2188MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2188-SP

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AF2344

Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

(17 Publications)

100 μg

AF2344

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2344U-100UG
Alexa Fluor 405	AF2344V-100UG
Alexa Fluor 488	AF2344G-100UG
Alexa Fluor 532	AF2344X-100UG
Alexa Fluor 594	AF2344T-100UG
Alexa Fluor 647	AF2344R-100UG
Alexa Fluor 700	AF2344N-100UG
Alexa Fluor 750	AF2344S-100UG
Alexa Fluor Plus 405	AF2344AFP405-100UG
Alexa Fluor Plus 488	AF2344AFP488-100UG
Alexa Fluor Plus 555	AF2344AFP555-100UG
Alexa Fluor Plus 594	AF2344AFP594-100UG
Alexa Fluor Plus 647	AF2344AFP647-100UG
Alexa Fluor Plus 680	AF2344AFP680-100UG
Allophycocyanin	AF2344A
Biotin	AF2344B
CoraFluor 1	AF2344CL1
DyLight 350	AF2344D
DyLight 405	AF2344E
DyLight 488	AF2344K
DyLight 550	AF2344L
DyLight 594	AF2344M
DyLight 650	AF2344W
DyLight 680	AF2344Y
DyLight 755	AF2344Z
FITC	AF2344F
HRP	AF2344H
Janelia Fluor 525	AF2344JF525
Janelia Fluor 549	AF2344I
Janelia Fluor 646	AF2344J
Janelia Fluor 669	AF2344JF669
PE	AF2344P
PerCP	AF2344C
mFluor Violet 450 SE	AF2344MFV450
mFluor Violet 500 SE	AF2344MFV500
mFluor Violet 610 SE	AF2344MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2344-SP

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MAB2188

Carbonic Anhydrase IX/CA9 Antibody (303123) [Unconjugated]

(8 Publications)

100 μg

MAB2188

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="U87-MG cells were stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

2188-CA) was serially diluted and captured by Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2188-CA) was serially diluted and captured by Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2188U-100UG
Alexa Fluor 405	FAB2188V-100UG
Alexa Fluor 488	FAB2188G-100UG
Alexa Fluor 532	FAB2188X
Alexa Fluor 594	FAB2188T-100UG
Alexa Fluor 647	FAB2188R-100UG
Alexa Fluor 700	FAB2188N-100UG
Alexa Fluor 750	FAB2188S-100UG
Alexa Fluor Plus 405	FAB2188AFP405-100UG
Alexa Fluor Plus 488	FAB2188AFP488-100UG
Alexa Fluor Plus 555	FAB2188AFP555-100UG
Alexa Fluor Plus 594	FAB2188AFP594-100UG
Alexa Fluor Plus 647	FAB2188AFP647-100UG
Alexa Fluor Plus 680	FAB2188AFP680-100UG
Allophycocyanin	FAB2188A
Allophycocyanin/Cy7	FAB2188APCCY7
Biotin	FAB2188B
CoraFluor 1	FAB2188CL1
DyLight 350	FAB2188D
DyLight 405	FAB2188E
DyLight 488	FAB2188K
DyLight 550	FAB2188L
DyLight 594	FAB2188M
DyLight 650	FAB2188W
DyLight 680	FAB2188Y
DyLight 755	FAB2188Z
Fluorescein	FAB2188F
HRP	FAB2188H
Janelia Fluor 525	FAB2188JF525
Janelia Fluor 549	FAB2188I
Janelia Fluor 646	FAB2188J
Janelia Fluor 669	FAB2188JF669
PE/Atto594	FAB2188PEATT594
PE/Cy5.5	FAB2188PECY55
PE/Cy7	FAB2188PECY7
PerCP	FAB2188C
Phycoerythrin	FAB2188P
mFluor Violet 450 SE	FAB2188MFV450
mFluor Violet 500 SE	FAB2188MFV500
mFluor Violet 610 SE	FAB2188MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2188F
Carrier Free	FAB2188P
Carrier Free	FAB2188A
Carrier Free	MAB2188-SP
Carrier Free	MAB2188-500
Carrier Free	FAB2188V-100UG
Carrier Free	FAB2188U-100UG
Carrier Free	FAB2188T-100UG
Carrier Free	FAB2188S-100UG
Carrier Free	FAB2188R-100UG
Carrier Free	FAB2188N-100UG
Carrier Free	FAB2188G-100UG

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NBP2-15199

Carbonic Anhydrase IX/CA9 Antibody (66.4.C2 (PN-15))

(6 Publications)

0.1 mg

NBP2-15199

Reactivity:

Hu, Eq

Species Glossary

Reactivity: Hu, Eq

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-130A

Alt Catalog# ABX-130A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33152AF350
Alexa Fluor 405	NBP2-33152AF405
Alexa Fluor 488	NBP2-33152AF488
Alexa Fluor 532	NBP2-33152AF532
Alexa Fluor 594	NBP2-33152AF594
Alexa Fluor 647	NBP2-33152AF647
Alexa Fluor 700	NBP2-33152AF700
Alexa Fluor 750	NBP2-33152AF750
Alexa Fluor Plus 405	NBP2-33152AFP405
Alexa Fluor Plus 488	NBP2-33152AFP488
Alexa Fluor Plus 555	NBP2-33152AFP555
Alexa Fluor Plus 594	NBP2-33152AFP594
Alexa Fluor Plus 647	NBP2-33152AFP647
Alexa Fluor Plus 680	NBP2-33152AFP680
Allophycocyanin	NBP2-33152APC
Biotin	NBP2-33152B-100ul
CoraFluor 1	NBP2-33152CL1
DyLight 350	NBP2-33152UV-100ul
DyLight 405	NBP2-33152V-100ul
DyLight 488	NBP2-33152G-100ul
DyLight 550	NBP2-33152R-100ul
DyLight 594	NBP2-33152DL594
DyLight 650	NBP2-33152C-100ul
DyLight 680	NBP2-33152FR-100ul
DyLight 755	NBP2-33152IR-100ul
FITC	NBP2-33152F
HRP	NBP2-33152H-100ul
Janelia Fluor 525	NBP2-33152JF525
Janelia Fluor 549	NBP2-33152JF549
Janelia Fluor 646	NBP2-33152JF646
Janelia Fluor 669	NBP2-33152JF669
PE	NBP2-33152PE
PerCP	NBP2-33152PCP
mFluor Violet 450 SE	NBP2-33152MFV450
mFluor Violet 500 SE	NBP2-33152MFV500
mFluor Violet 610 SE	NBP2-33152MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33152
IHC-Prediluted	NBP2-48405

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MAB21881

Carbonic Anhydrase IX/CA9 Antibody (303104)

(1 Publication)

100 μg

MAB21881

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB21881U-100UG
Alexa Fluor 405	FAB21881V-100UG
Alexa Fluor 488	FAB21881G-100UG
Alexa Fluor 532	FAB21881X-100UG
Alexa Fluor 594	FAB21881T-100UG
Alexa Fluor 647	FAB21881R-100UG
Alexa Fluor 700	FAB21881N-100UG
Alexa Fluor 750	FAB21881S-100UG
Alexa Fluor Plus 405	FAB21881AFP405-100UG
Alexa Fluor Plus 488	FAB21881AFP488-100UG
Alexa Fluor Plus 555	FAB21881AFP555-100UG
Alexa Fluor Plus 594	FAB21881AFP594-100UG
Alexa Fluor Plus 647	FAB21881AFP647-100UG
Alexa Fluor Plus 680	FAB21881AFP680-100UG
Allophycocyanin	FAB21881A
Biotin	FAB21881B
CoraFluor 1	FAB21881CL1
DyLight 350	FAB21881D
DyLight 405	FAB21881E
DyLight 488	FAB21881K
DyLight 550	FAB21881L
DyLight 594	FAB21881M
DyLight 650	FAB21881W
DyLight 680	FAB21881Y
DyLight 755	FAB21881Z
FITC	FAB21881F
HRP	FAB21881H
Janelia Fluor 525	FAB21881JF525
Janelia Fluor 549	FAB21881I
Janelia Fluor 646	FAB21881J
Janelia Fluor 669	FAB21881JF669
PE	FAB21881P
PerCP	FAB21881C
mFluor Violet 450 SE	FAB21881MFV450
mFluor Violet 500 SE	FAB21881MFV500
mFluor Violet 610 SE	FAB21881MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB21881-SP

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MAB11526

Carbonic Anhydrase IX/CA9 Antibody (1349A) [Unconjugated]

100 μg

MAB11526

VCTS021). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB11526) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB11526) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11526U-100UG
Alexa Fluor 405	FAB11526V-100UG
Alexa Fluor 488	FAB11526G-100UG
Alexa Fluor 532	FAB11526X-100UG
Alexa Fluor 594	FAB11526T-100UG
Alexa Fluor 647	FAB11526R-100UG
Alexa Fluor 700	FAB11526N-100UG
Alexa Fluor 750	FAB11526S-100UG
Alexa Fluor Plus 405	FAB11526AFP405-100UG
Alexa Fluor Plus 488	FAB11526AFP488-100UG
Alexa Fluor Plus 555	FAB11526AFP555-100UG
Alexa Fluor Plus 594	FAB11526AFP594-100UG
Alexa Fluor Plus 647	FAB11526AFP647-100UG
Alexa Fluor Plus 680	FAB11526AFP680-100UG
Allophycocyanin	FAB11526A
Biotin	FAB11526B
CoraFluor 1	FAB11526CL1
DyLight 350	FAB11526D
DyLight 405	FAB11526E
DyLight 488	FAB11526K
DyLight 550	FAB11526L
DyLight 594	FAB11526M
DyLight 650	FAB11526W
DyLight 680	FAB11526Y
DyLight 755	FAB11526Z
FITC	FAB11526F
HRP	FAB11526H
Janelia Fluor 525	FAB11526JF525
Janelia Fluor 549	FAB11526I
Janelia Fluor 646	FAB11526J
Janelia Fluor 669	FAB11526JF669
PE	FAB11526P
PerCP	FAB11526C
mFluor Violet 450 SE	FAB11526MFV450
mFluor Violet 500 SE	FAB11526MFV500
mFluor Violet 610 SE	FAB11526MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11526-SP

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NBP2-54743

Carbonic Anhydrase IX/CA9 Antibody - BSA Free

100 ul

NBP2-54743

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human colon shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human duodenum shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human gallbladder shows strong membranous positivity in glandular cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-54743-25ul

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NBP2-45238

Carbonic Anhydrase IX/CA9 Antibody (PN-15)

0.1 mg

NBP2-45238

Reactivity:

Hu, Eq

Species Glossary

Reactivity: Hu, Eq

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11592AF350
Alexa Fluor 405	NBP3-11592AF405
Alexa Fluor 488	NBP3-11592AF488
Alexa Fluor 532	NBP3-11592AF532
Alexa Fluor 594	NBP3-11592AF594
Alexa Fluor 647	NBP3-11592AF647
Alexa Fluor 700	NBP3-11592AF700
Alexa Fluor 750	NBP3-11592AF750
Alexa Fluor Plus 405	NBP3-11592AFP405
Alexa Fluor Plus 488	NBP3-11592AFP488
Alexa Fluor Plus 555	NBP3-11592AFP555
Alexa Fluor Plus 594	NBP3-11592AFP594
Alexa Fluor Plus 647	NBP3-11592AFP647
Alexa Fluor Plus 680	NBP3-11592AFP680
Allophycocyanin	NBP3-11592APC
Biotin	NBP3-11592B
CoraFluor 1	NBP3-11592CL1
DyLight 350	NBP3-11592UV
DyLight 405	NBP3-11592V
DyLight 488	NBP3-11592G
DyLight 550	NBP3-11592R
DyLight 594	NBP3-11592DL594
DyLight 650	NBP3-11592C
DyLight 680	NBP3-11592FR
DyLight 755	NBP3-11592IR
FITC	NBP3-11592F
HRP	NBP3-11592H
Janelia Fluor 525	NBP3-11592JF525
Janelia Fluor 549	NBP3-11592JF549
Janelia Fluor 646	NBP3-11592JF646
Janelia Fluor 669	NBP3-11592JF669
PE	NBP3-11592PE
PerCP	NBP3-11592PCP
mFluor Violet 450 SE	NBP3-11592MFV450
mFluor Violet 500 SE	NBP3-11592MFV500
mFluor Violet 610 SE	NBP3-11592MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-11592

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NBP2-45237

Carbonic Anhydrase IX/CA9 Antibody (CA9/781)

0.1 mg

NBP2-45237

Reactivity:

Hu, Eq

Species Glossary

Reactivity: Hu, Eq

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11591AF350
Alexa Fluor 405	NBP3-11591AF405
Alexa Fluor 488	NBP3-11591AF488
Alexa Fluor 532	NBP3-11591AF532
Alexa Fluor 594	NBP3-11591AF594
Alexa Fluor 647	NBP3-11591AF647
Alexa Fluor 700	NBP3-11591AF700
Alexa Fluor 750	NBP3-11591AF750
Alexa Fluor Plus 405	NBP3-11591AFP405
Alexa Fluor Plus 488	NBP3-11591AFP488
Alexa Fluor Plus 555	NBP3-11591AFP555
Alexa Fluor Plus 594	NBP3-11591AFP594
Alexa Fluor Plus 647	NBP3-11591AFP647
Alexa Fluor Plus 680	NBP3-11591AFP680
Allophycocyanin	NBP3-11591APC
Biotin	NBP3-11591B
CoraFluor 1	NBP3-11591CL1
DyLight 350	NBP3-11591UV
DyLight 405	NBP3-11591V
DyLight 488	NBP3-11591G
DyLight 550	NBP3-11591R
DyLight 594	NBP3-11591DL594
DyLight 650	NBP3-11591C
DyLight 680	NBP3-11591FR
DyLight 755	NBP3-11591IR
FITC	NBP3-11591F
HRP	NBP3-11591H
Janelia Fluor 525	NBP3-11591JF525
Janelia Fluor 549	NBP3-11591JF549
Janelia Fluor 646	NBP3-11591JF646
Janelia Fluor 669	NBP3-11591JF669
PE	NBP3-11591PE
PerCP	NBP3-11591PCP
mFluor Violet 450 SE	NBP3-11591MFV450
mFluor Violet 500 SE	NBP3-11591MFV500
mFluor Violet 610 SE	NBP3-11591MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-11591

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NBP3-49540

Carbonic Anhydrase IX/CA9 Antibody (rCA9/9937) - Azide and BSA Free

100 μg

NBP3-49540

Western Blot Analysis of Human Stomach tissue cell lysate using Carbonic Anhydrase IX/CA9 Antibody (rCA9/9937) - Azide and BSA Free.

Formalin-fixed, paraffin-embedded human stomach stained with Carbonic Anhydrase IX/CA9 Antibody (rCA9/9937) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Western Blot Analysis of HeLa cell lysate using Carbonic Anhydrase IX/CA9 Antibody (rCA9/9937) - Azide and BSA Free.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Carbonic Anhydrase IX/CA9 Antibodies

Carbonic Anhydrase IX/CA9 Antibody - BSA Free

Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

Carbonic Anhydrase IX/CA9 Antibody (303123) [Unconjugated]

Carbonic Anhydrase IX/CA9 Antibody (66.4.C2 (PN-15))

Carbonic Anhydrase IX/CA9 Antibody (303104)

Carbonic Anhydrase IX/CA9 Antibody (1349A) [Unconjugated]

Carbonic Anhydrase IX/CA9 Antibody - BSA Free

Carbonic Anhydrase IX/CA9 Antibody (PN-15)

Carbonic Anhydrase IX/CA9 Antibody (CA9/781)

Carbonic Anhydrase IX/CA9 Antibody (rCA9/9937) - Azide and BSA Free