Glycophorin A Antibody (R10) [Unconjugated]

Glycophorin A was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of red blood cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: R10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glycophorin A Antibody (R10) [Unconjugated] Summary

• Immunogen: Abelson mouse leukemia virus-induced human pre-B tumor cells
• Specificity: Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GYPA

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1.7-25 ug/mL
  • Immunoprecipitation
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycophorin A Antibody (R10) [Unconjugated]

• CD235a antigen
• CD235a
• glycophorin A (includes MN blood group)
• glycophorin A (MNS blood group)
• Glycophorin A
• glycophorin Erik
• glycophorin MiI
• glycophorin MiIII
• glycophorin MiV
• glycophorin MiX
• glycophorin SAT
• glycophorin Sta type C
• glycophorin-A
• GPA
• GPErik
• GpMiIII
• GPSAT
• GYPA
• HGpMiIII
• HGpMiV
• HGpMiX
• HGpMiXI
• HGpSta(C)
• Mi.V glycoprotein (24 AA)
• MN sialoglycoprotein
• MNS
• PAS-2
• recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

1. Greaves, M.F. et al. (1983) Blood 61(4):645.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycophorin A Antibody (MAB1228)(2)

Publications using MAB1228

• Estes ML, Mund JA, Mead LE Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A, 2010;77(9):831-9. 2010 [PMID: 20803735] (Flow Cytometry, Human)
• DiMeglio LA, Tosh A, Saha C Endothelial abnormalities in adolescents with type 1 diabetes: a biomarker for vascular sequelae? J. Pediatr., 2010;157(4):540-6. 2010 [PMID: 20542287] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: GYPA