GST Epitope Tag Antibody

Images

 
Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the HRP conjugate of NB600-326. Detection of 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml ...read more
Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).
Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the Biotin conjugate of NB600-326. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml ...read more
Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed ...read more
Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST ...read more
Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This ...read more
Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

GST Epitope Tag Antibody Summary

Immunogen
Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:30000
  • Simple Western 1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-326 in the following applications:

Publications
Read Publications using
NB600-326 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GST Epitope Tag Antibody

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GST Epitope Tag Antibody (NB600-326)(17)

We have publications tested in 2 confirmed species: Rat, Bacteria.

We have publications tested in 3 applications: IHC-P, IP, WB.


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IHC-P
(1)
IP
(1)
WB
(5)
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Rat
(1)
Bacteria
(1)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB600-326 Applications Species
Chen Z, Morales JE, Guerrero PA et al. PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells Cancer Res. May 9 2018 [PMID: 29743287] (WB) WB
Kuchler P, Zimmermann G, Winzker M et al. Identification of novel PDEd interacting proteins Bioorg. Med. Chem. 2017 Aug 31 [PMID: 28935183] (IP) IP
Jalouli M, Mokas S, Turgeon C et al. Selective HIF-1 Regulation under Nonhypoxic Conditions by the p42/p44 MAP Kinase Inhibitor PD184161. Mol Pharmacol 2017 Aug 16 [PMID: 28814529] (WB) WB
Mokas S, Lariviere R, Lamalice L et al. Hypoxia-inducible factor-1 plays a role in phosphate-induced vascular smooth muscle cell calcification Kidney Int. [PMID: 27470678] (WB, Bacteria) WB Bacteria
Chandler BD, Burkhardt AL, Foley K et al. A Fully Synthetic and Biochemically Validated Phosphatidyl Inositol-3-Phosphate Hapten via Asymmetric Synthesis and Native Chemical Ligation J Am Chem Soc 2014 Jan 8 [PMID: 24344932] (WB)

Details:
This citation used the HRP version of this antibody.
WB
Pilar AV, Reid-Yu SA, Cooper CA et al. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog 2012 [PMID: 22761574]
Iorio-Morin C, Germain P, Roy S et al. Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism. Cell Death Differ 2012 Aug [PMID: 22343716]
Mason FM, Heimsath EG, Higgs HN et al. Bi-modal regulation of a formin by srGAP2 J Biol Chem 2011 Feb [PMID: 21148482]

Details:
This citation used the HRP version of this antibody.
Parent A, Roy SJ, Iorio-Morin C et al. ANKRD13C acts as a molecular chaperone for G protein-coupled receptors. J Biol Chem 2010 Dec [PMID: 20959461]
Fujita A, Cheng J, Tauchi-Sato K et al. A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique. Proc Natl Acad Sci U S A 2009 Jun [PMID: 19470488]
Show All 17 Publications.

Reviews for GST Epitope Tag Antibody (NB600-326) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB600-326:
Filter by Applications
WB
(2)
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Filter by Species
Other
(2)
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Images Ratings Applications Species Date Details
Western Blot GST Epitope Tag NB600-326
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reviewed by:
Anonymous
WB Other 01/07/2016
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Summary

ApplicationWestern Blot
Sample TestedGST fustion protein
SpeciesOther

Blocking

Blocking Details-

Details

Detection Notes-
Western Blot GST Epitope Tag NB600-326
Enlarge
reviewed by:
Darren Richard
WB Other 07/24/2015
View

Summary

ApplicationWestern Blot
Sample TestedGST pulldowns
SpeciesOther

Blocking

Blocking DetailsTBS-T, Milk, 2h, room temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG
Secondary Manufacturer Cat#Promega
Secondary Concentration1:3000

Details

Detection NotesHomemade ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GST Epitope Tag Antibody (NB600-326) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB600-326B
FITC Labeled NB600-326F
HRP Labeled NB600-388

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
01/07/2016
Application: WB
Species: Other

Darren Richard
07/24/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol GSTM1