GST Epitope Tag Antibody - BSA Free

Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).

• Reactivity: ET
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GST Epitope Tag Antibody - BSA Free Summary

• Immunogen: Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSTM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
  • Simple Western 1:2000
  • Western Blot 1:1000 - 1:30000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in hUbiquitn-GST and hISG15-GST lysates, hVAV1-GST, E. coli lysate and hPARK2-GST, conditioned media, separated by Size, antibody dilution of 1:2000, apparent MW was 39, 47, 43, 81 kDa. IHC-P reactivity reported in (PMID: 19412421).

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808). Bacteria reported in (PMID: 27470678).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GST Epitope Tag Antibody - BSA Free

• EC 2.5.1.18
• glutathione S-alkyltransferase
• glutathione S-aralkyltransferase
• glutathione S-aryltransferase
• glutathione S-transferase M1
• glutathione S-transferase mu 1
• GST class-mu 1
• GST HB subunit 4
• GST1
• GSTM1-1
• GSTM1a-1a
• GSTM1b-1b
• GTH4
• GTM1
• HB subunit 4
• H-B
• MGC26563
• MU
• MU-1
• S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GST Epitope Tag Antibody (NB600-326)(18)

Publications using NB600-326

• Greulich BM, Rajendran S, Downing NF et al. A complex with poly(A)-binding protein and EWS facilitates the transcriptional function of oncogenic ETS transcription factors in prostate cells The Journal of biological chemistry 2023-11-11 [PMID: 37956771] (WB)
• Pasquini M, Grosjean N, Hixson KK et al. Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase Cell reports 2022-05-17 [PMID: 35584675]
• Chen Z, Morales JE, Guerrero PA et al. PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells Cancer Res. 2018-05-09 [PMID: 29743287] (WB)
• Kuchler P, Zimmermann G, Winzker M et al. Identification of novel PDEd interacting proteins Bioorg. Med. Chem. 2017-08-31 [PMID: 28935183] (IP)
• Jalouli M, Mokas S, Turgeon CA et al. Selective HIF-1 Regulation under Nonhypoxic Conditions by the p42/p44 MAP Kinase Inhibitor PD184161. Mol Pharmacol. [PMID: 28814529] (WB, Rat)
• Mokas S, Lariviere R, Lamalice L et al. Hypoxia-inducible factor-1 plays a role in phosphate-induced vascular smooth muscle cell calcification Kidney Int. [PMID: 27470678] (WB, Bacteria)
• Chandler BD, Burkhardt AL, Foley K et al. A Fully Synthetic and Biochemically Validated Phosphatidyl Inositol-3-Phosphate Hapten via Asymmetric Synthesis and Native Chemical Ligation J Am Chem Soc 2014-01-08 [PMID: 24344932] (WB)
  
  Details:
  This citation used the HRP version of this antibody.
• Rodriguez Pena MJ, Castillo Bennett JV et al. MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis. PLoS One 2013-05-21 [PMID: 23704996] (WB)
  
  Details:
  Citation using the Bulk Size format of this antibody.
• Pilar AV, Reid-Yu SA, Cooper CA et al. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog 2012-01-01 [PMID: 22761574]
• Iorio-Morin C, Germain P, Roy S et al. Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism. Cell Death Differ 2012-08-01 [PMID: 22343716]
• Mason FM, Heimsath EG, Higgs HN et al. Bi-modal regulation of a formin by srGAP2 J Biol Chem 2011-02-01 [PMID: 21148482]
  
  Details:
  This citation used the HRP version of this antibody.
• Parent A, Roy SJ, Iorio-Morin C et al. ANKRD13C acts as a molecular chaperone for G protein-coupled receptors. J Biol Chem 2010-12-01 [PMID: 20959461]
• Fujita A, Cheng J, Tauchi-Sato K et al. A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique. Proc Natl Acad Sci U S A 2009-06-01 [PMID: 19470488]
• Ghosh R, Schoolfield J, Yeh IT et al. Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development. Transl Oncol 2009-05-01 [PMID: 19412421] (IHC-P, Rat)
• Plafker KS, Farjo KM, Wiechmann AF et al. The human ubiquitin conjugating enzyme, UBE2E3, is required for proliferation of retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 2008-12-01 [PMID: 18614808]
• Nirmala J, Dahl S, Steffenson BJ et al. Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance. Proc Natl Acad Sci U S A 2007-06-01 [PMID: 17548826]
• Hamelin E, Theriault C, Laroche G et al. The intracellular trafficking of the G protein-coupled receptor TPbeta depends on a direct interaction with Rab11. J Biol Chem 2005-10-01 [PMID: 16126723]
• Rochdi MD, Laroche G, Dupre E et al. Nm23-H2 interacts with a G protein-coupled receptor to regulate its endocytosis through an Rac1-dependent mechanism. J Biol Chem 2004-04-01 [PMID: 14976202]

Reviews for GST Epitope Tag Antibody (NB600-326) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Verified Customer WB Other 01/07/2016

Summary

• Application: Western Blot
• Sample Tested: GST fustion protein
• Species: Other

reviewed by: Darren Richard WB Other 07/24/2015

Summary

• Application: Western Blot
• Sample Tested: GST pulldowns
• Species: Other

Bioinformatics

• Gene Symbol: GSTM1