GST Epitope Tag Antibody

Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the HRP conjugate of NB600-326. Detection of 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).

Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the Biotin conjugate of NB600-326. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 60 seconds.

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).

Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).

• Reactivity: NA
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

GST Epitope Tag Antibody Summary

• Immunogen: Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSTM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:30000
  • Simple Western 1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GST Epitope Tag Antibody

• EC 2.5.1.18
• glutathione S-alkyltransferase
• glutathione S-aralkyltransferase
• glutathione S-aryltransferase
• glutathione S-transferase M1
• glutathione S-transferase mu 1
• GST class-mu 1
• GST HB subunit 4
• GST1
• GSTM1-1
• GSTM1a-1a
• GSTM1b-1b
• GTH4
• GTM1
• HB subunit 4
• H-B
• MGC26563
• MU
• MU-1
• S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GST Epitope Tag Antibody (NB600-326)(17)

Publications using NB600-326

• Chen Z, Morales JE, Guerrero PA et al. PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells Cancer Res. May 9 2018 [PMID: 29743287] (WB)
• Chandler BD, Burkhardt AL, Foley K et al. A Fully Synthetic and Biochemically Validated Phosphatidyl Inositol-3-Phosphate Hapten via Asymmetric Synthesis and Native Chemical Ligation J Am Chem Soc (WB)
  
  Details:
  This publication used the HRP conjugated form of this antibody (Cat# NB600-388).
• Kuchler P, Zimmermann G, Winzker M et al. Identification of novel PDEd interacting proteins Bioorg. Med. Chem. 2017 Aug 31 [PMID: 28935183] (IP)
• Jalouli M, Mokas S, Turgeon C et al. Selective HIF-1 Regulation under Nonhypoxic Conditions by the p42/p44 MAP Kinase Inhibitor PD184161. Mol Pharmacol 2017 Aug 16 [PMID: 28814529] (WB)
• Mokas S, Lariviere R, Lamalice L et al. Hypoxia-inducible factor-1 plays a role in phosphate-induced vascular smooth muscle cell calcification Kidney Int. [PMID: 27470678] (WB, Bacteria)
• Chandler BD, Burkhardt AL, Foley K et al. A Fully Synthetic and Biochemically Validated Phosphatidyl Inositol-3-Phosphate Hapten via Asymmetric Synthesis and Native Chemical Ligation J Am Chem Soc 2014 Jan 8 [PMID: 24344932] (WB)
  
  Details:
  This citation used the HRP version of this antibody.
• Iorio-Morin C, Germain P, Roy S et al. Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism. Cell Death Differ 2012 Aug [PMID: 22343716]
• Mason FM, Heimsath EG, Higgs HN et al. Bi-modal regulation of a formin by srGAP2 J Biol Chem 2011 Feb [PMID: 21148482]
  
  Details:
  This citation used the HRP version of this antibody.
• Parent A, Roy SJ, Iorio-Morin C et al. ANKRD13C acts as a molecular chaperone for G protein-coupled receptors. J Biol Chem 2010 Dec [PMID: 20959461]
• Fujita A, Cheng J, Tauchi-Sato K et al. A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique. Proc Natl Acad Sci U S A 2009 Jun [PMID: 19470488]
• Ghosh R, Schoolfield J, Yeh IT et al. Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development. Transl Oncol 2009 May [PMID: 19412421] (IHC-P, Rat)
• Plafker KS, Farjo KM, Wiechmann AF et al. The human ubiquitin conjugating enzyme, UBE2E3, is required for proliferation of retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 2008 Dec [PMID: 18614808]
• Nirmala J, Dahl S, Steffenson BJ et al. Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance. Proc Natl Acad Sci U S A 2007 Jun [PMID: 17548826]
• Hamelin E, Theriault C, Laroche G et al. The intracellular trafficking of the G protein-coupled receptor TPbeta depends on a direct interaction with Rab11. J Biol Chem 2005 Oct [PMID: 16126723]
• Rochdi MD, Laroche G, Dupre E et al. Nm23-H2 interacts with a G protein-coupled receptor to regulate its endocytosis through an Rac1-dependent mechanism. J Biol Chem 2004 Apr [PMID: 14976202]
• Mason FM, Heimsath EG, Higgs HN et al. Bi-modal regulation of a formin by srGAP2 J Biol Chem
  
  Details:
  This publication used the HRP conjugated form of this antibody (Cat# NB600-388).
• Pilar AV, Reid-Yu SA, Cooper CA et al. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog 2012 [PMID: 22761574]

Reviews for GST Epitope Tag Antibody (NB600-326) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Anonymous WB Other 01/07/2016

Summary

• Application: Western Blot
• Sample Tested: GST fustion protein
• Species: Other

Blocking

• Blocking Details: -

Details

• Detection Notes: -

reviewed by: Darren Richard WB Other 07/24/2015

Summary

• Application: Western Blot
• Sample Tested: GST pulldowns
• Species: Other

Blocking

• Blocking Details: TBS-T, Milk, 2h, room temperature

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG
• Secondary Manufacturer Cat#: Promega
• Secondary Concentration: 1:3000

Details

• Detection Notes: Homemade ECL

Bioinformatics

• Gene Symbol: GSTM1