c-Myc Antibody


Western Blot: c-Myc Antibody [NB600-336] - Analysis using the HRP conjugate of NB600-336. Detection of c-Myc in NIH/3T3 cell lysates (30ug and 45ug per lane) using anti-c-Myc antibody. Image from verified customer ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [NB600-336] - ICC/IF staining of K-562, U266, Daudi and normal T cells with c-Myc antibody. Image from verified customer review.
Immunohistochemistry: c-Myc Antibody [NB600-336] - Analysis of c-Myc in mouse prostate using DAB with hematoxylin counterstain.
Western Blot: c-Myc Antibody [NB600-336] - c-Myc in MOLT-4 cells. Image from verified customer review.
Western Blot: c-Myc Antibody [NB600-336] - Analysis of c-Myc on Jurkat whole cell extract using NB600-336.
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [NB600-336] - Detection of c-myc Tagged Plakoglobin by Immunofluorescence. Samples: Human microvascular endothelial cells expressing c-myc tagged plakoglobin ...read more
Simple Western: c-Myc Antibody [NB600-336] - Simple Western lane view shows a specific band for c-Myc in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
1.37 mg/ml

c-Myc Antibody Summary

A synthetic peptide made to the human c-Myc protein (between residues 385-435) [UniProt P01106]
Cytoplasmic and Nuclear
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.09% Sodium Azide
1.37 mg/ml
Immunogen affinity purified


Application Notes
This c-Myc antibody is useful for ELISA, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation, and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
c-Myc Peptide (NB600-336PEP)
Reviewed Applications
Read 2 Reviews rated 5
NB600-336 in the following application:

Read Publications using
NB600-336 in the following applications:

  • 1 publication
  • 3 publications
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Non-species specific when used to detect the c-Myc tag. When used to detect the c-Myc protein this antibody reacts with human and mouse.

Alternate Names for c-Myc Antibody

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)


Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for c-Myc Antibody (NB600-336)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-336 Applications Species
Clairfeuille T, Norwood SJ, Qi X et al. Structure and membrane binding properties of the endosomal tetratricopeptide repeat (TPR) domain-containing sorting nexins SNX20 and SNX21 J. Biol. Chem. 2015 Apr 16 [PMID:25882846]
Naffin-Olivos JL, Georgieva M, Goldfarb N et al. Mycobacterium tuberculosis Hip1 Modulates Macrophage Responses through Proteolysis of GroEL2. PLoS Pathog. 2014 May 01 [PMID:24830429] (WB) WB
Lin YN, Wu CT, Lin YC et al. CEP120 interacts with CPAP and positively regulates centriole elongation. J Cell Biol 2013 Jul 22 [PMID:23857771] (ICC/IF) ICC/IF
Lin YC, Chang CW, Hsu WB et al. Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assembly EMBO J 2013 Mar 19 [PMID:23511974] (IP, Human) IP Human
Tomalka AG, Stopford CM, Lee PC, Rietsch A. A translocator-specific export signal establishes the translocator-effector secretion hierarchy that is important for type III secretion system function Mol Microbiol 2012 Dec [PMID:23121689] (WB) WB
Tanaka A, Tanizawa H, Sriswasdi S, et al. Epigenetic Regulation of Condensin-Mediated Genome Organization during the Cell Cycle and upon DNA Damage through Histone H3 Lysine 56 Acetylation Mol Cell 2012 Oct 17 [PMID:23084836] (ChIP) ChIP
Fontaine SN, Bauer SP, Lin X et al. Replacement of charged and polar residues in the coiled-coiled interface of huntingtin-interacting protein 1 (HIP1) causes aggregation and cell death FEBS Lett 2012 Jul 23 [PMID:22835334] (WB, Human) WB Human
Park YK, Park H. Differentiated embryo chondrocyte 1 (DEC1) represses PPAR?2 gene through interacting with CCAAT/enhancer binding protein ? (C/EBP?). Mol Cells. 2012 May 18. [PMID:22610404] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Tang C, Lin S, Hsu W et al. The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation. EMBO J. 2011 [PMID:22020124]
Zheng Q, Su H, Ranek MJ, Wang X. Autophagy and p62 in Cardiac Proteinopathy. Circ Res. 2011 Jun 9. [PMID:21659648] (ICC/IF, Mouse) ICC/IF Mouse
Show All 13 Publications.

Reviews for c-Myc Antibody (NB600-336) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: WB.

Reviews using NB600-336:
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Images Ratings Applications Species Date Details
 c-Myc NB600-336
reviewed by:
Human 10/02/2015


Sample TestedK-562, U266, Daudi, normal human T cells


Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/50 1ary antibody diluted in 10% donkey serum/PBS overnight at 4C

Secondary Antibody

Secondary DescriptionDonkey F(ab)2 anti-rabbit IgG (H+L) Alexa Fluor 488
Secondary Manufacturer Cat#Jackson 711-546-152
Secondary Concentration1/1000


Detection NotesZeiss AxioImager, FITC filter cube for 250 ms exposure
Fixation DetailsImmersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step
Western Blot c-Myc NB600-336
reviewed by:
WB Human 07/24/2014


ApplicationWestern Blot
Sample TestedWB for c-Myc in MOLT-4 cells
CommentsThe antibody gives a strong signal. Would work good even with lesser lysate.


Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:20,000


Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 5 sec


CommentsThe antibody gives a strong signal. Would work good even with lesser lysate.

Product General Protocols

View specific protocols for c-Myc Antibody (NB600-336): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-336). (Showing 1 - 1 of 1 FAQs).

  1. I want to know the MW of NB600-336.
    • This antibody is designed to detect c-Myc which is a common epitope tag used on proteins. The MW detected with each protein will vary based on the size of the protein. The c-Myc tag is only about 1.2kDa but the full length Myc protein is about 49kDa.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

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Blogs on c-Myc. Showing 1-10 of 12 blog posts - Show all blog posts.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol MYC

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