Use Of c-Myc Antibodies In Non-Invasive Cancer Studies

Mon, 05/17/2010 - 07:33

Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.

C-Myc is one of several transcription factor proteins covered by our antibody catalog. Encoded by the MYC gene, c-Myc is important for cell differentiation, proliferation, cycle progression and apoptosis. It is expressed in a number of different cancers.

The phosphorylation of c-Myc has been widely studied in order to better understand its oncogenic properties. Antibody assays have suggested a functional link between c-Myc phosphorylation at Thr58/Ser62, Glycogen Synthase Kinsase 3 (GSK3), Mitogen-Activated Protein Kinase 1 (ERK2), C-Jun N terminal Kinase (JNK) and cyclin dependent kinase (CDK). In addition, it has been suggested that c-Myc is essential for the angiogenesis (i.e. new growth) of blood vessels in tumors.

Western Blot: c-Myc Antibody Western Blot: c-Myc Antibody

Human breast cancer cells commonly display raised c-Myc and insulin-like growth factor 1 receptor (IGF1R) levels owing to over-expression of messenger RNA. In 2005, Tian, et al. established a non-invasive molecular imaging technique to detect MYC mRNA levels in human breast cancer cells (from the MCF-7 cell-line) grafted onto nude mice.

Scintigraphy is a diagnostic method widely used to track radioisotopes in human patients. Tian used this to detect MYC probes tagged with IGF1 and a [99mTc] PNA (peptide nucleic acid) chimera peptide. The idea was to see if levels of MYC mRNA could be evaluated in over-expressed IGF1 cancer cells, using radio-labelled [99mTc]PNA antibody probes. The results were successful, paving the way for further research and proving how important antibody catalogs like ours are to clinical research.

Novus Biologicals offers many c-Myc reagents for your research needs including:

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