Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in human heart tissue.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein A purified
Western Blot 1:500-1:1000
Chromatin Immunoprecipitation 1:10-1:500
Gel Super Shift Assays 1:1-1:100
This n-Myc (NMYC-1) antibody is useful for Immunocytochemistry/ Immunofluorescence, Immunoprecipitation and Western Blot, where a band can been seen at approximately 50-65 kDa. This antibody has also been used for Chromatin Immunoprecipitation (PMID: 21068393) and Gel Super Shift Assays (PMID: 21068393). Use in FLOW cytometry reported in scientific literature (PMID 27539729).
neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
NMYCneuroblastoma MYC oncogene
v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
n-Myc, also known as bHLHe37, NMYC, N-myc proto-oncogene protein, and MYCN, is a transcriptional regulator of cell proliferation, differentiation and apoptosis. n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Defects in n-Myc are the cause of Feingold syndrome type 1 (FGLDS1), a syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. n-Myc expression is highest during embryonic development and then later in the adult during B-cell development. These expression patterns and results from targeted deletion of n-Myc suggest that n-Myc plays an important role in tissue development and differentiation. Recent research has linked n-Myc to suppressing the anti-apoptotic protein Bcl-2 (PMID: 22920753), as well as elucidated this proteins role in various forms of tumorigenesis (PMID: 22722710).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.