n-Myc Antibody (NMYC-1)

Images

 
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IP, CyTOF-ready
Clone
NMYC-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

n-Myc Antibody (NMYC-1) Summary

Immunogen
A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
Localization
Nuclear, some cytoplasmic.
Specificity
This antibody does not react with c-Myc or L-Myc.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYCN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunoprecipitation 1:10 - 1:100
  • CyTOF-ready
Application Notes
In Western blot a band can been seen at ~50-65 kDa. This antibody has also been used for Chromatin Immunoprecipitation (PMID: 21068393) and Gel Super Shift Assays (PMID: 21068393). Use in FLOW cytometry reported in scientific literature (PMID 27539729).
Control
n-Myc Overexpression Lysate
Publications
Read Publications using
NB200-109 in the following applications:

  • 1 publication
  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for n-Myc Antibody (NMYC-1)

  • BHLHE37
  • bHLHe37N-myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 37
  • MODED
  • neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
  • N-myc
  • NMYCneuroblastoma MYC oncogene
  • ODED
  • oncogene NMYC
  • pp65/67
  • v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc, also known as bHLHe37, NMYC, N-myc proto-oncogene protein, and MYCN, is a transcriptional regulator of cell proliferation, differentiation and apoptosis. n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Defects in n-Myc are the cause of Feingold syndrome type 1 (FGLDS1), a syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. n-Myc expression is highest during embryonic development and then later in the adult during B-cell development. These expression patterns and results from targeted deletion of n-Myc suggest that n-Myc plays an important role in tissue development and differentiation. Recent research has linked n-Myc to suppressing the anti-apoptotic protein Bcl-2 (PMID: 22920753), as well as elucidated this proteins role in various forms of tumorigenesis (PMID: 22722710).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-109
Species: Hu, Mu
Applications: WB, ChIP, Flow, GS, ICC/IF, IP, CyTOF-ready

Publications for n-Myc Antibody (NB200-109)(5)

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Product General Protocols

View specific protocols for n-Myc Antibody (NB200-109): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for n-Myc Antibody (NB200-109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-109AF350
Alexa Fluor 405 NB200-109AF405
Alexa Fluor 488 NB200-109AF488
Alexa Fluor 532 NB200-109AF532
Alexa Fluor 594 NB200-109AF594
Alexa Fluor 647 NB200-109AF647
Alexa Fluor 700 NB200-109AF700
Alexa Fluor 750 NB200-109AF750
Allophycocyanin NB200-109APC
Biotin NB200-109B
DyLight 350 NB200-109UV
DyLight 405 NB200-109V
DyLight 488 NB200-109G
DyLight 550 NB200-109R
DyLight 594 NB200-109DL594
DyLight 650 NB200-109C
DyLight 680 NB200-109FR
DyLight 755 NB200-109IR
FITC NB200-109F
HRP NB200-109H
Janelia Fluor 549 NB200-109JF549
Janelia Fluor 646 NB200-109JF646
PE NB200-109PE

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Blogs on n-Myc.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

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Bioinformatics

Gene Symbol MYCN
Entrez
Uniprot