n-Myc Antibody (NMYC-1)

Images

 
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in human heart tissue.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IP
Clone
NMYC-1
Clonality
Monoclonal
Host
Mouse
Concentration
1.0 mg/ml

n-Myc Antibody (NMYC-1) Summary

Immunogen
A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
Localization
Nuclear, some cytoplasmic.
Specificity
This does not react with c-Myc or L-Myc.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYCN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:100
Application Notes
This n-Myc (NMYC-1) antibody is useful for Immunocytochemistry/ Immunofluorescence, Immunoprecipitation and Western Blot, where a band can been seen at approximately 50-65 kDa. This antibody has also been used for Chromatin Immunoprecipitation (PMID: 21068393) and Gel Super Shift Assays (PMID: 21068393). Use in FLOW cytometry reported in scientific literature (PMID 27539729).
Publications
Read Publications using
NB200-109 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse. Not yet tested in other species.

Alternate Names for n-Myc Antibody (NMYC-1)

  • BHLHE37
  • bHLHe37N-myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 37
  • MODED
  • neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
  • N-myc
  • NMYCneuroblastoma MYC oncogene
  • ODED
  • oncogene NMYC
  • pp65/67
  • v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc, also known as bHLHe37, NMYC, N-myc proto-oncogene protein, and MYCN, is a transcriptional regulator of cell proliferation, differentiation and apoptosis. n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Defects in n-Myc are the cause of Feingold syndrome type 1 (FGLDS1), a syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. n-Myc expression is highest during embryonic development and then later in the adult during B-cell development. These expression patterns and results from targeted deletion of n-Myc suggest that n-Myc plays an important role in tissue development and differentiation. Recent research has linked n-Myc to suppressing the anti-apoptotic protein Bcl-2 (PMID: 22920753), as well as elucidated this proteins role in various forms of tumorigenesis (PMID: 22722710).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for n-Myc Antibody (NB200-109)(3)

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Product General Protocols

View specific protocols for n-Myc Antibody (NB200-109): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for n-Myc Antibody (NB200-109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB200-109V
FITC Labeled NB200-109F

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Blogs on n-Myc

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MYCN
Entrez
Uniprot

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