n-Myc Antibody (NMYC-1) - BSA Free

Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.

Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, Flow, GS, ICC/IF, IP, CyTOF-ready
• Clone: NMYC-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

n-Myc Antibody (NMYC-1) - BSA Free Summary

• Immunogen: A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
• Localization: Nuclear, some cytoplasmic.
• Specificity: This antibody does not react with c-Myc or L-Myc.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYCN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
  • Flow Cytometry
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunoprecipitation 1:10 - 1:100
  • Western Blot 1:500 - 1:1000
• Application Notes: In Western blot a band can been seen at ~50-65 kDa. This antibody has also been used for Chromatin Immunoprecipitation (PMID: 21068393) and Gel Super Shift Assays (PMID: 21068393). Use in FLOW cytometry reported in scientific literature (PMID 27539729).
• Control:

  n-Myc Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for n-Myc Antibody (NMYC-1) - BSA Free

• BHLHE37
• bHLHe37N-myc proto-oncogene protein
• Class E basic helix-loop-helix protein 37
• MODED
• neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
• N-myc
• NMYCneuroblastoma MYC oncogene
• ODED
• oncogene NMYC
• pp65/67
• v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
• v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc, also known as bHLHe37, NMYC, N-myc proto-oncogene protein, and MYCN, is a transcriptional regulator of cell proliferation, differentiation and apoptosis. n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Defects in n-Myc are the cause of Feingold syndrome type 1 (FGLDS1), a syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. n-Myc expression is highest during embryonic development and then later in the adult during B-cell development. These expression patterns and results from targeted deletion of n-Myc suggest that n-Myc plays an important role in tissue development and differentiation. Recent research has linked n-Myc to suppressing the anti-apoptotic protein Bcl-2 (PMID: 22920753), as well as elucidated this proteins role in various forms of tumorigenesis (PMID: 22722710).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for n-Myc Antibody (NB200-109)(6)

Publications using NB200-109

• Ton AT, Foo J, Singh K et al. Development of VPC-70619, a Small-Molecule N-Myc Inhibitor as a Potential Therapy for Neuroendocrine Prostate Cancer International journal of molecular sciences 2022-02-26 [PMID: 35269731] (PLA, Human)
• Gupta R, Turati V, Brian D et Al. Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity Cell Stem Cell. [PMID: 32197066] (FLOW, Human)
• Yang Y, Ding L, Zhou Q et al. Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells Cancer Cell Int. 2020-01-07 [PMID: 31920463] (WB, Mouse)
• Murakami-Tonami Y, Ikeda H, Yamagishi R et al. SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci Rep. 2016-08-19 [PMID: 27539729] (ChIP, FLOW, WB, Human)
• Murakami-Tonami Y, Kishida S, Takeuchi I et al. Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle 2014-01-01 [PMID: 24553121]
• Tasseva G, Cole L, Vance JE. N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells. J Biol Chem 286(2):1061-73. 2011-01-14 [PMID: 21068393]

Bioinformatics

• Gene Symbol: MYCN
• Entrez:
  • 4613(Human)
  • 18109(Mouse)
• Uniprot:
  • P04198(Human)