Epitope Tags and the V5 Tag Antibody

Wed, 01/12/2011 - 07:17

We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid residues 95-108 of the simian virus 5 RNA polymerase alpha subunit.

Epitope tagging is widely used in proteomics and genetic studies. The epitope is, of course, the portion of the antigen protein to which the antibody binds. An epitope tag is an artificial epitope that is recognized by a common antibody. The genetic sequence of the tag is engineered using recombinant DNA techniques, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody raised to that epitope then recognizes it, enabling the tagged protein to be isolated, purified, visualized and analyzed by immunochemical methods.

Western Blot: V5-TAG Antibody

Epitope tags are short sequences of around 10 to 15 amino acids. Our V5 tag antibody is 14 amino acids long and is normally used in this form, though a shorter sequence of nine amino acids has been used. Like all anti-epitope tag sequences it is engineered onto the N- or C- terminus of the protein of interest. This minimizes structural disruptions which may affect the protein’s function, and allows immunochemical visualization and analysis.

In 2009 V5 tag antibody assays were part of a study demonstrating that distinct classes of chromatin-interacting membrane proteins have a shared function at the end of mitosis. Another study linked oestrogen receptor pathways to the reading disorder dyslexia. We have 21 V5 tag antibody products in our antibody catalog, giving plenty of scope for researchers.

Novus Biologicals offers many V5 tag reagents for your research needs including:

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