The lysate was created in HEK293T cells, using plasmid ID RC201611 and based on accession number NM_002467. The protein contains a C-terminal DDK tag.
Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug) and an empty vector negative control (100 ug).
50.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBL1-13414 in the following application:
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
FAQs for c-Myc Lysate (NBL1-13414). (Showing 1 - 1 of 1 FAQ).
What amount of 'over-expression lysate for c-myc' did you use in this example image for the western blot?
The Western blot you see on the datasheet represents the blot from a gel wherein 5ug of lysate # NBL1-13414 was loaded. However, to our customers, we generally recommend using 5-10ug of lysate because the antibody that we used in our validation of the lysate might be different from what customers would be using. Please make sure to heat the 5-10ug lysate aliquot (to be loaded) at 95-100 degrees Celsius for 5 minutes along with your other test samples before loading on to the gel.
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