Max Antibody

Images

 
Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Max Antibody [NB100-793] - Staining of Prostate. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more
Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Max Antibody Summary

Immunogen
Peptide with sequence C-EEPQSRKKLRMEAS corresponding to C-Terminus according to NP_002373.3, NP_660087.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Immunofluorescence 10 ug/mL
Application Notes
WB: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3).
Control
Jurkat Whole Cell Lysate
Max Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-793 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine. Rat reactivity reported in scientific literature (PMID: 24676840).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Max Antibody

  • BHLHD4
  • bHLHd4MGC11225
  • bHLHd5
  • bHLHd6
  • bHLHd7
  • bHLHd8
  • Class D basic helix-loop-helix protein 4
  • helix-loop-helix zipper protein
  • MAX protein
  • Max
  • MGC10775
  • MGC18164
  • MGC34679
  • MGC36767
  • MYC associated factor X
  • Myc-associated factor X
  • orf1
  • protein max

Background

A basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD - regulating gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Max Antibody (NB100-793)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Max Antibody (NB100-793) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Max Antibody (NB100-793)

Discover related pathways, diseases and genes to Max Antibody (NB100-793). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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Research Areas for Max Antibody (NB100-793)

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Blogs on Max.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

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Bioinformatics

Gene Symbol MAX
Entrez
Uniprot