Genetic Strategies: Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control.  protein standard ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal ...read more
Immunohistochemistry: c-Fos Antibody (2H2) [NBP2-50037] - pAb 1:1000 (green), DAPI counterstain (blue) on 30 micron cryosection of mouse spinal cord. This image was submitted via customer Review.
Biological Strategies: Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation ...read more
In Western blot, this antibody recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos.
50-65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Immunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.
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