c-Fos Antibody (2H2)

Images

 
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, ICC/IF
Clone
2H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

c-Fos Antibody (2H2) Summary

Immunogen
Recombinant full length human c-Fos. [UniProt# P01100]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
In Western blot, this antibody recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-50037 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Fos Antibody (2H2)

  • activator protein 1
  • AP-1
  • cellular oncogene c-fos
  • Cellular oncogene fos
  • cFos
  • c-Fos
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
  • FBJ murine osteosarcoma viral oncogene homolog
  • FOS
  • G0/G1 switch regulatory protein 7
  • G0S7
  • proto-oncogene c-Fos
  • v-fos FBJ murine osteosarcoma viral oncogene homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Fos Antibody (NBP2-50037) (0)

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Review for c-Fos Antibody (NBP2-50037) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-50037:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry c-Fos NBP2-50037
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reviewed by:
Peter Caradonna
ICC Mouse 06/20/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult spinal cord
SpeciesMouse

Comments

CommentsImmunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Fos Antibody (NBP2-50037). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for some c-fos antibodies that could work in IHC.
    • Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for c-Fos Antibody (NBP2-50037)

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Recent Reviews

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1
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Peter Caradonna
06/20/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol FOS
Entrez
Uniprot