c-Fos Antibody (2H2)


Biological Strategies: Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal ...read more
Genetic Strategies: Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard ...read more
Immunohistochemistry: c-Fos Antibody (2H2) [NBP2-50037] - pAb 1:1000 (green), DAPI counterstain (blue) on 30 micron cryosection of mouse spinal cord. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
1 mg/ml

Order Details

c-Fos Antibody (2H2) Summary

This c-Fos Antibody (2H2) was developed against full length recombinant human c-Fos protein expressed in and purified from E. coli. [UniProt# P01100]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Knockout Validated
  • Western Blot 1:1000 - 1:2000
Theoretical MW
50-65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
NBP2-50037 in the following applications:

Read Publications using
NBP2-50037 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33091429).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% PBS, 50% glycerol
5mM Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for c-Fos Antibody (2H2)

  • activator protein 1
  • AP-1
  • cellular oncogene c-fos
  • Cellular oncogene fos
  • cFos
  • c-Fos
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
  • FBJ murine osteosarcoma viral oncogene homolog
  • Fos proto-oncogene, AP-1 trancription factor subunit
  • FOS
  • G0/G1 switch regulatory protein 7
  • G0S7
  • p55
  • proto-oncogene c-Fos


c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB (1-3). Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa (4,5). c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex (3,5). AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites (5).

In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation (3,6). A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress (1,6). c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli (1-3, 6-7). Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development (3). Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear (2,7). Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal (7). Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model (2). Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression (2).


1. Kovacs K. J. (1998). c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochemistry International. https://doi.org/10.1016/s0197-0186(98)00023-0

2. Gallo, F. T., Katche, C., Morici, J. F., Medina, J. H., & Weisstaub, N. V. (2018). Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Frontiers in Behavioral Neuroscience. https://doi.org/10.3389/fnbeh.2018.00079

3. Velazquez, F. N., Caputto, B. L., & Boussin, F. D. (2015). c-Fos importance for brain development. Aging. https://doi.org/10.18632/aging.100862

4. Uniprot (P01100)

5. Wu, Z., Nicoll, M., & Ingham, R. J. (2021). AP-1 family transcription factors: a diverse family of proteins that regulate varied cellular activities in classical hodgkin lymphoma and ALK+ ALCL. Experimental Hematology & Oncology. https://doi.org/10.1186/s40164-020-00197-9

6. Shaulian, E., & Karin, M. (2001). AP-1 in cell proliferation and survival. Oncogene. https://doi.org/10.1038/sj.onc.1204383

7. Chung L. (2015). A Brief Introduction to the Transduction of Neural Activity into Fos Signal. Development & Reproduction. https://doi.org/10.12717/DR.2015.19.2.061


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Fos Antibody (NBP2-50037)(18)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP2-50037 Applications Species
Zhai J, Li X, Hao H et al. Whisker Stimulation Alleviate ASD Behavior of BTBR Mice by Regulating Piezo2 Expression in DRG and Neuron Function in S1 and mPFC SSRN Electronic Journal 2022-12-20
Nagai Y, Kisaka Y, Nomura K et al. Dorsal raphe serotonergic neurons preferentially reactivate dorsal dentate gyrus cell ensembles associated with positive experience Cell reports 2023-02-22 [PMID: 36821440] (IHC, Mouse) IHC Mouse
Nagaoka K, Asaoka N, Nagayasu K et al. Enhancement of adenosine A2A signaling improves dopamine D2 receptor antagonist-induced dyskinesia via ?-arrestin signaling Frontiers in neuroscience 2023-01-24 [PMID: 36760795] (IHC-Fr, Mouse) IHC-Fr Mouse
Bao C, Huang J, Wu H et al. Moxibustion alleviates depression-like behavior in rats with Crohn's disease by inhibiting the kynurenine pathway metabolism in the gut-brain axis Frontiers in neuroscience 2022-12-07 [PMID: 36570839] (WB, Rat) WB Rat
Kawai H, Bouchekioua Y, Nishitani N et al. Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion Nature communications 2022-12-22 [PMID: 36550097] (IHC-Fr, Mouse)

Dilution used in IHC-Fr 1:200
IHC-Fr Mouse
Nagaoka K, Nagayasu K, Shirakawa H, Kaneko S Acetaminophen improves tardive akathisia induced by dopamine D2 receptor antagonists Journal of Pharmacological Sciences 2022-10-01 [PMID: 36522124] (IF/IHC, Rat) IF/IHC Rat
Takayama K, Tobori S, Andoh C et al. Autism Spectrum Disorder Model Mice Induced by Prenatal Exposure to Valproic Acid Exhibit Enhanced Empathy-Like Behavior via Oxytocinergic Signaling Biological & pharmaceutical bulletin 2022-07-31 [PMID: 35908894] (IHC-Fr, Mouse)

Dilution used for IHC 1:500
IHC-Fr Mouse
Li Y, Zhu S, Xie K et al. TLR4 in Tph2 neurons modulates anxiety-related behaviors in a sex-dependent manner Neuropharmacology 2022-09-15 [PMID: 35787402] (ICC/IF)

Dilution: 1:1000
Huang ST, Chen BB, Song ZJ et al. Unraveling the role of Epac1-SOCS3 signaling in the development of neonatal-CRD-induced visceral hypersensitivity in rats CNS neuroscience & therapeutics 2022-06-15 [PMID: 35702948] (IF/IHC, Rat) IF/IHC Rat
Figueroa C, Yang H, DiSpirito J Et al. Morphine exposure alters Fos expression in a sex-, age-, and brain region-specific manner during adolescence Developmental psychobiology 2021-09-01 [PMID: 34423851] (IF/IHC, Rat) IF/IHC Rat
Show All 18 Publications.

Review for c-Fos Antibody (NBP2-50037) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-50037:
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Images Ratings Applications Species Date Details
Immunocytochemistry c-Fos NBP2-50037
reviewed by:
Peter Caradonna
ICC Mouse 06/20/2017


Sample TestedAdult spinal cord


CommentsImmunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.

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  1. I am looking for some c-fos antibodies that could work in IHC.
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Recent Reviews


Peter Caradonna
Application: ICC
Species: Mouse


Gene Symbol FOS