Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation ...read more
Immunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.
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