c-Fos Antibody (2H2)

Images

 
Genetic Strategies: Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal ...read more
Immunohistochemistry: c-Fos Antibody (2H2) [NBP2-50037] - pAb 1:1000 (green), DAPI counterstain (blue) on 30 micron cryosection of mouse spinal cord. This image was submitted via customer Review.
Biological Strategies: Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clone
2H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

c-Fos Antibody (2H2) Summary

Immunogen
Full length recombinant human c-Fos protein expressed in and purified from E. coli. [UniProt# P01100]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Knockout Validated
Application Notes
In Western blot, this antibody recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos.
Theoretical MW
50-65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
c-Fos Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-50037 in the following applications:

Publications
Read Publication using
NBP2-50037 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Fos Antibody (2H2)

  • activator protein 1
  • AP-1
  • cellular oncogene c-fos
  • Cellular oncogene fos
  • cFos
  • c-Fos
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
  • FBJ murine osteosarcoma viral oncogene homolog
  • FOS
  • G0/G1 switch regulatory protein 7
  • G0S7
  • proto-oncogene c-Fos
  • v-fos FBJ murine osteosarcoma viral oncogene homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
H00001958-M03
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-47757
Species: Hu
Applications: WB, IHC, IHC-P
NB300-273
Species: Mu, Rt
Applications: WB, IHC, IHC-Fr
AF8691
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
NBP2-82026
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
MAB4640
Species: Hu
Applications: WB
NBP2-37568
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-81575
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO

Publications for c-Fos Antibody (NBP2-50037)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for c-Fos Antibody (NBP2-50037) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-50037:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry c-Fos NBP2-50037
Enlarge
reviewed by:
Peter Caradonna
ICC Mouse 06/20/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult spinal cord
SpeciesMouse

Comments

CommentsImmunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Fos Antibody (NBP2-50037). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for some c-fos antibodies that could work in IHC.
    • Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-50037AF405
Alexa Fluor 488 NBP2-50037AF488
Alexa Fluor 647 NBP2-50037AF647
Alexa Fluor 700 NBP2-50037AF700
Biotin NBP2-50037B
DyLight 350 NBP2-50037UV
DyLight 405 NBP2-50037V
DyLight 488 NBP2-50037G
DyLight 550 NBP2-50037R
DyLight 650 NBP2-50037C
DyLight 680 NBP2-50037FR
DyLight 755 NBP2-50037IR
FITC NBP2-50037F
HRP NBP2-50037H
Janelia Fluor 549 NBP2-50037JF549
Janelia Fluor 646 NBP2-50037JF646

Additional c-Fos Products

Bioinformatics Tool for c-Fos Antibody (NBP2-50037)

Discover related pathways, diseases and genes to c-Fos Antibody (NBP2-50037). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for c-Fos Antibody (NBP2-50037)

Discover more about diseases related to c-Fos Antibody (NBP2-50037).
 

Pathways for c-Fos Antibody (NBP2-50037)

View related products by pathway.

PTMs for c-Fos Antibody (NBP2-50037)

Learn more about PTMs related to c-Fos Antibody (NBP2-50037).
 

Research Areas for c-Fos Antibody (NBP2-50037)

Find related products by research area.

Blogs on c-Fos.

The C99 fragment of amyloid precursor protein (APP)
Alzheimer’s Disease (AD) is a neurodegenerative disorder that is characterized by an abundance of the beta-amyloid peptide in the brain.  When AD was first discovered, it was determined that beta-amyloid was produced as a result of the prote...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Peter Caradonna
06/20/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol FOS
Uniprot