HRAS Antibody

Images

 
Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Analysis of mouse prostate. H-Ras antibody dilution: 1:500.
Western Blot: HRAS Antibody [NBP2-42864] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE H-Ras antibody dilution: 1:1000
Western Blot: HRAS Antibody [NBP2-42864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Analysis of mouse intestine. H-Ras antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HRAS Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.
Specificity
This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
Predicted Species
Rhesus Macaque (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HRAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Application Notes
IP Assay dependent
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for HRAS Antibody (NBP2-42864)

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Bioinformatics

Gene Symbol HRAS