HRAS Antibody - BSA Free

Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: HRAS Antibody [NBP2-42864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:5000.

Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: HRAS Antibody [NBP2-42864] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Western Blot: HRAS Antibody [NBP2-42864] -Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HRAS Antibody [NBP2-42864] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HRAS Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HRAS. The exact sequence is proprietary.
• Specificity: This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
• Predicted Species: Xenopus (100%), Chicken (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HRAS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:10000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HRAS Antibody - BSA Free

• C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HRAS Antibody (NBP2-42864)(2)

Publications using NBP2-42864

• Ko A, Hasanain M, Oh YT et al. LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL Cancer discovery 2022-11-29 [PMID: 36445254]
• Moser R, Gurley KE, Nikolova O et al. Synthetic lethal kinases in Ras/p53 mutant squamous cell carcinoma Oncogene 2022-05-10 [PMID: 35538224] (WB, Mouse)

Bioinformatics

• Gene Symbol: HRAS