Bcl-xL Antibody

Images

 
Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. ...read more
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The ...read more
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-xL at ~30 kDa.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in human breast carcinoma at 1:2000. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Bv, Ma, ShSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Bcl-xL Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 46-66 (ETPSAINGNPSWHLADSPAVN) of human Bcl-xL was used as immunogen; GenBank no. CAA80661.1. The immunogen sequence is 100% conserved in Bcl-XS. The immunogen sequence is 100% in multiple species including mouse, rat, dog, cow, gerbil, and sheep.
Specificity
Bcl-XL+S
Predicted Species
Bovine (100%), Gerbil (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Control
Bcl-xL Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-56104 in the following applications:

Publications
Read Publication using
NB100-56104 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Bcl-xL Antibody

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56104 recognizes Bcl-xL and Bcl-xS. Human Bcl-xL is a 233 amino acid protein; GenBank no. CAA80661.1. Human Bcl-xS is a 170 amino acid protein; GenBank no. CAA80662.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-xL Antibody (NB100-56104)(1)

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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Mouse
(1)
Rat
(1)
Canine
(1)
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Review for Bcl-xL Antibody (NB100-56104) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56104:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Bcl-xL NB100-56104
Enlarge
4
reviewed by:
Anonymous
WB Human 07/15/2016
View

Summary

ApplicationWestern Blot
Sample TestedDaoy whole cell lysate (25ug)
SpeciesHuman

Blocking

Blocking Details5% Milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:2000 in 5% milk PBST overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit IgG HRP
Secondary Manufacturer Cat#Southern Biotech 6440-05
Secondary Concentration1:5000

Details

Detection NotesAdvansta WesternBright ECL

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Bcl-xL Antibody (NB100-56104) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Bcl-xL.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol BCL2L1
Entrez