Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF


1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and 220-250 kDa, more
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is 20-80 ng/mL in the presence of 0.6 µg/mL Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129).
Mouse myeloma cell line, NS0-derived human ErbB2/Her2 protein
Human Erb B2
Accession # NP_004439
6 His-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
96.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
125-130 kDa, reducing conditions
220-250 kDa, non-reducing conditions
Read Publications using
1129-ER in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)


ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.

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Publications for ErbB2/Her2 (1129-ER)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Drosophila, N/A.

We have publications tested in 9 applications: Bioassay, DirELISA, ELISA Standard, ELISA capture, ELISA development, Flow, SPR, Surface Plasmon Resonance, WB Standard.

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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 1129-ER Applications Species
M Pruszynski, CM Kang, E Koumariano, G Vaidyanath, MR Zalutsky d-Amino Acid Peptide Residualizing Agents for Protein Radioiodination: Effect of Aspartate for Glutamate Substitution Molecules, 2018;23(5):. 2018 [PMID: 29783774] (Bioassay, N/A) Bioassay N/A
M Kahl, F Settele, P Knick, U Haupts, E Bosse-Doen Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts Protein Expr. Purif., 2018;0(0):. 2018 [PMID: 29704557] (Bioassay) Bioassay
V Tolmachev, TJ Grönroos, CB Yim, J Garousi, Y Yue, S Grimm, J Rajander, A Perols, M Haaparanta, O Solin, R Ferdani, A Orlova, CJ Anderson, AE Karlström Molecular design of radiocopper-labelled Affibody molecules Sci Rep, 2018;8(1):6542. 2018 [PMID: 29695813] (SPR, Human) SPR Human
M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors Theranostics, 2017;7(10):2620-2633. 2017 [PMID: 28819451] (ELISA development, N/A) ELISA development N/A
N Shinozaki, R Hashimoto, K Fukui, S Uchiyama Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities Sci Rep, 2017;7(1):5794. 2017 [PMID: 28725057] (Bioassay, N/A) Bioassay N/A
B Petrovic, T Gianni, V Gatta, G Campadelli Insertion of a ligand to HER2 in gB retargets HSV tropism and obviates the need for activation of the other entry glycoproteins PLoS Pathog., 2017;13(4):e1006352. 2017 [PMID: 28423057] (Bioassay, Human) Bioassay Human
J Tobias, J Jasinska, K Baier, M Kundi, N Ede, C Zielinski, U Wiedermann Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide BMC Cancer, 2017;17(1):118. 2017 [PMID: 28183282] (Bioassay, Mouse) Bioassay Mouse
A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (DirELISA, Human) DirELISA Human
Sachdev S Sidhu A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (WB Standard) WB Standard
Peng Dai Salt Effect Accelerates Site-Selective Cysteine Bioconjugation ACS Cent Sci, 2016;2(9):637-646. 2016 [PMID: 27725962] (Bioassay) Bioassay
Show All 28 Publications.

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FAQs for ErbB2/Her2 (1129-ER). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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Gene Symbol ERBB2