Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

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1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and 220-250 kDa, ...read more
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875.

The ED50 for this effect is 20-80 ng/mL in the presence of 0.6 µg/mL goat anti-hErbB2 (Catalog # AF1129).

Source
Mouse myeloma cell line, NS0-derived
Human Erb B2
(Thr23 - Thr652)
Accession # NP_004439
IEGRMD Human IgG1
(Pro100 - Lys330)
6 His-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Thr23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ERBB2
Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
96.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE

125-130 kDa, reducing conditions

220-250 kDa, non-reducing conditions

Publications
Read Publications using
1129-ER in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1 - 10).

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1129-ER
Species: Hu
Applications: BA

Publications for ErbB2/Her2 (1129-ER)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Drosophila, N/A.

We have publications tested in 7 applications: Bioassay, DirELISA, ELISA Standard, ELISA capture, ELISA development, Flow, Surface Plasmon Resonance.


Filter By Application
Bioassay
(14)
DirELISA
(1)
ELISA Standard
(1)
ELISA capture
(1)
ELISA development
(2)
Flow
(3)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(13)
Mouse
(3)
Drosophila
(1)
N/A
(5)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 1129-ER Applications Species
M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors Theranostics, 2017;7(10):2620-2633. 2017 [PMID: 28819451] (ELISA development, N/A) ELISA development N/A
N Shinozaki, R Hashimoto, K Fukui, S Uchiyama Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities Sci Rep, 2017;7(1):5794. 2017 [PMID: 28725057] (Bioassay, N/A) Bioassay N/A
B Petrovic, T Gianni, V Gatta, G Campadelli Insertion of a ligand to HER2 in gB retargets HSV tropism and obviates the need for activation of the other entry glycoproteins PLoS Pathog., 2017;13(4):e1006352. 2017 [PMID: 28423057] (Bioassay, Human) Bioassay Human
J Tobias, J Jasinska, K Baier, M Kundi, N Ede, C Zielinski, U Wiedermann Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide BMC Cancer, 2017;17(1):118. 2017 [PMID: 28183282] (Bioassay, Mouse) Bioassay Mouse
A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (DirELISA, Human) DirELISA Human
F Selis, G Focà, A Sandomenic, C Marra, C Di Mauro, G Saccani Jo, S Scaramuzza, A Politano, R Sanna, M Ruvo, G Tonon Pegylated Trastuzumab Fragments Acquire an Increased in Vivo Stability but Show a Largely Reduced Affinity for the Target Antigen Int J Mol Sci, 2016;17(4):. 2016 [PMID: 27043557] (ELISA capture, Human) ELISA capture Human
Kanojia D, Balyasnikova I, Morshed R, Frank R, Yu D, Zhang L, Spencer D, Kim J, Han Y, Yu D, Ahmed A, Aboody K, Lesniak M Neural Stem Cells Secreting Anti-HER2 Antibody Improve Survival in a Preclinical Model of HER2 Overexpressing Breast Cancer Brain Metastases. Stem Cells, 2015;33(10):2985-94. 2015 [PMID: 26260958] (Bioassay, Human) Bioassay Human
Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, Carvalho J, Gaspar M, Weller S, Medcalf M, Wydro M, Pegram R, Mudde G, Bauer A, Moulder K, Woisetschlager M, Tuna M, Haurum J, Sun H A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis. Mol Ther, 2015;23(11):1722-33. 2015 [PMID: 26234505] (Bioassay, Human) Bioassay Human
Zhou Q, Uhlig K, Muth A, Kimpel J, Levy C, Munch R, Seifried J, Pfeiffer A, Trkola A, Coulibaly C, von Laer D, Wels W, Hartwig U, Verhoeyen E, Buchholz C Exclusive Transduction of Human CD4+ T Cells upon Systemic Delivery of CD4-Targeted Lentiviral Vectors. J Immunol, 2015;195(5):2493-501. 2015 [PMID: 26232436] (Flow, Human) Flow Human
VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice. Mol Ther, 2015;23(10):1600-10. 2015 [PMID: 26122933] (Bioassay, Mouse) Bioassay Mouse
Show All 23 Publications.

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FAQs for ErbB2/Her2 (1129-ER). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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Bioinformatics

Gene Symbol ERBB2
Entrez
Uniprot