ABCA1 Antibody - BSA Free

Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Knockdown Validated: ABCA1 Antibody [NB400-105] - HSKMCs were transfected with scrambled siRNA (scr siRNA) or ABCA1 siRNA. The cells were lysed and ABCA1 (A) protein levels were quantified by immunoblotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-38014-3) licensed under under a CC-BY licence.

Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. Representative image of ovarian cancer showing high (left panel) and low (right panel) ABCA1 expression on the cell membrane or cytoplasm (x400). Image collected and cropped by CiteAb from the following publication (//www.clinicalepigeneticsjournal.com/content/7/1/1), licensed under a CC-BY licence.

Western Blot: ABCA1 Antibody [NB400-105] - ABCA1 in human primary fibroblasts in culture. 10 ug of total protein. 7.5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4C. Secondary 1:5000 HRP 1h RT. Arrow shows around 250 kDa. WB image submitted by a verified customer review.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

Western Blot: ABCA1 Antibody [NB400-105] - Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high cholesterol diet were analyzed by western blotting with antibodies against ABCA1, LDLR and ACTIN as a loading control (left panel), quantification of ABCA1 and LDLR protein accumulation levels (right panel). * p<0.05, *** p<0.001 in Student's t test. Error bars represent the +/- mean SEM. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003483), licensed under a CC-BY licence.

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ mice. Representative images are shown. beta-actin was used as the loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY licence.

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b regulates ABCA1 and SREBP-1. Analysis for ABCA1, SREBP-1, CPT1a, and AMPK alpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are shown. TF2B and beta-actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

• Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, Rb
• Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ABCA1 Antibody - BSA Free Summary

• Immunogen: Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.
• Localization: Astrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation
  • Dual RNAscope ISH-IHC
  • ELISA
  • Flow Cytometry 1:400
  • Gel Supershift Assay
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated
  • PCR
  • Simple Western 1:50
  • Western Blot 1:500
• Application Notes: This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID: 15684432). Use in immunoblotting reported in scientific literature (PMID: 27599291). Use in blocking/ neutralizing reported in scientific literature (PMID: 30821416). Use in PCR reported in scientific literature (PMID: 27406916). Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation (PMID: 19515742) and ELISA (PMID: 18541924) were also reported in scientific literature. Knockdown validation (PMID: 31666189). Knockout validation (PMID: 32273567). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Charge.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raw 264.7 Whole Cell - T0901317 treated Assay Kit
  THP-1 TO901317 Treated / Untreated Cell Lysate

• Control Peptide:

  ABCA1 Protein (NB400-105PEP)

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA1 Antibody - BSA Free

• ABC1
• ABC-1
• ABC1ATP-binding cassette transporter A1
• ABCA1
• ATP-binding cassette 1
• ATP-binding cassette transporter 1
• ATP-binding cassette, sub-family A (ABC1), member 1
• CERP
• Cholesterol efflux regulatory protein
• EC 2.7.7.8
• EC 2.8.1.8
• EC 3.6.3
• EC 3.6.3.41
• FLJ14958
• HDLDT1
• membrane-bound
• MGC164864
• MGC165011
• TGD
• TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA1 Antibody (NB400-105)(379)

Publications using NB400-105

• Papotti B, Adorni MP, Marchi C et al. PCSK9 Affects Astrocyte Cholesterol Metabolism and Reduces Neuron Cholesterol Supplying In Vitro: Potential Implications in Alzheimer's Disease International journal of molecular sciences Oct 13 2022 [PMID: 36293049] (WB, Human)
• Cassim Bawa FN, Gopoju R, Xu Y et al. Retinoic Acid Receptor Alpha (RARa) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice Cells Oct 11 2022 [PMID: 36291054] (WB, Mouse)
• Cai Y, Wang Z, Li L et al. Neuropeptide Y regulates cholesterol uptake and efflux in macrophages and promotes foam cell formation Journal of cellular and molecular medicine Sep 29 2022 [PMID: 36172879] (WB, Human)
• Man B, Hu C, Yang G et al. Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPAR alpha in ApoE-/- mice Biochemical and Biophysical Research Communications Jul 1 2022 [PMID: 35933927] (WB, Mouse)
• Wang JQ, Li LL, Hu A et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Nature Aug 1 2022 [PMID: 35922515] (WB)
  
  Details:
  Dilution: 1:1000
• Perelroizen R, Philosof B, Budick-Harmelin N et al. Astrocyte immunometabolic regulation of the tumour microenvironment drives glioblastoma pathogenicity Brain : a journal of neurology Jul 28 2022 [PMID: 35899587] (IHC-Fr, WB, Mouse)
• Wang W, Lokman N, Noye T et al. ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome Cancer Drug Resist May 18 2022 [PMID: 35582032]
• Toma L, Barbalata T, Sanda G Et al. CRISPR/dCas9 Transcriptional Activation of Endogenous Apolipoprotein AI and Paraoxonase 1 in Enterocytes Alleviates Endothelial Cell Dysfunction Biomolecules Nov 25 2021 [PMID: 34944413] (WB, Human)
• Han Y, Onufer E, Huang L et al. Enterically derived high-density lipoprotein restrains liver injury through the portal vein Science Jul 23 2021 [PMID: 34437091]
• Dubland JA. Low lysosomal acid lipase activity in smooth muscle cells relative to macrophages provides new insights into foam cell formation in atherosclerosis. Thesis. 2018 (WB, Human)
• Vaisar T, Wang S, Omer M et al. 10,12-conjugated linoleic acid supplementation improves HDL composition and function in mice Journal of lipid research Jun 14 2022 [PMID: 35714730] (WB, Mouse)
• Picataggi A, Rodrigues A, Cromley DA et al. Specificity of ABCA7-mediated cell lipid efflux Biochimica et biophysica acta. Molecular and cell biology of lipids Jul 1 2022 [PMID: 35381375] (WB, Human)
• Peters F, Ebner LJA, Atac D et al. Regulation of ABCA1 by AMD-Associated Genetic Variants and Hypoxia in iPSC-RPE International journal of molecular sciences Mar 16 2022 [PMID: 35328615] (WB, Human)
• Yildirim Z, Baboo S, Hamid SM et al. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression EMBO molecular medicine Feb 22 2022 [PMID: 35191199] (WB, Mouse)
• Balamurugan K, Medishetti R, Kotha J et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways iScience Feb 1 2022 [PMID: 35141506] (WB, Mouse, Human)
• Kane J, Jansen M, Hendrix S et al. Anti-Galectin-2 antibody treatment reduces atherosclerotic plaque size and alters macrophage polarity Thrombosis and haemostasis Dec 1 2021 [PMID: 34852377] (IHC, Mouse)
• Casado ME, Huerta L, Marcos-DIaz A Et al. Hormone-sensitive lipase deficiency affects the expression of SR-BI, LDLr, and ABCA1 receptors/transporters involved in cellular cholesterol uptake and efflux and disturbs fertility in mouse testis Biochimica et biophysica acta. Molecular and cell biology of lipids Aug 27 2021 [PMID: 34461308] (IHC, Mouse)
• Wright MB, Varona Santos J, Kemmer C Et al. Compounds targeting OSBPL7 increase ABCA1-dependent cholesterol efflux preserving kidney function in two models of kidney disease Nature communications Aug 2 2021 [PMID: 34341345] (IHC, Mouse)
• Mota AC, Dominguez M, Weigert A et al. Lysosome-Dependent LXR and PPAR delta Activation Upon Efferocytosis in Human Macrophages Frontiers in immunology May 7 2021 [PMID: 34025647] (WB, Human)
• Zhang S, Li Z, Zhang Y et al. Ketone Body 3 Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a Mediated Calcium Influx Advanced Science Mar 1 2021 [PMID: 33977048] (WB, IHC-P, Mouse)
• Navia-Pelaez JM, Choi SH, Dos Santos Aggum Capettini L et al. Normalization of cholesterol metabolism in spinal microglia alleviates neuropathic pain The Journal of experimental medicine Jul 5 2021 [PMID: 33970188]
• Wang H, Arias EB, Cartee GD Reduced Membrane Cholesterol Content in Skeletal Muscle Is Not Essential for Greater Insulin-stimulated Glucose Uptake after Acute Exercise by Rats Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme Mar 25 2021 [PMID: 33765397]
• Brampton C, Pomozi V, Chen LH et al. ABCC6 deficiency promotes dyslipidemia and atherosclerosis Scientific reports Feb 16 2021 [PMID: 33594095] (ICC/IF, Mouse)
• Horie T, Nakao T, Miyasaka Y et al. microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity Nature communications Feb 16 2021 [PMID: 33594062] (WB, Mouse)
• Alajbegovic A, Holmberg J, Daoud F, et al. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells Vascular pharmacology Jan 28 2021 [PMID: 33516965] (WB, Human)
• Lv JJ, Wang H, Cui HY, et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis Frontiers in cell and developmental biology Jan 8 2020 [PMID: 33490072] (WB, ICC/IF, Mouse)
• Palafox-Sanchez V, Ying Z, Royes LFF, Gomez-Pinilla F The interaction between brain and liver regulates lipid metabolism in the TBI pathology Biochimica et biophysica acta. Molecular basis of disease Jan 12 2021 [PMID: 33444711] (WB, Rat)
• Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology Dec 14 2020 [PMID: 33381046] (WB, Mouse)
• Ren Q, Xie X, Tang Y et al. Methyl tertiary-butyl ether inhibits THP-1 macrophage cholesterol efflux in vitro and accelerates atherosclerosis in ApoE-deficient mice in vivo J Environ Sci (China) Dec 18 2020 [PMID: 33334519]
• Mukherjee V, Ramakrishna P, Bora S, Kotteazeth S Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation Steroids Nov 7 2020 [PMID: 33171131] (WB, Human)
  
  Details:
  Western blot analysis performed on HepG2 cells.
• Dodge JC, Jensen EH, Yu J et al. Neutral lipid cacostasis contributes to disease pathogenesis in amyotrophic lateral sclerosis J Neurosci Oct 13 2020 [PMID: 33051352]
• Munir MT, Ponce C, Santos JM et al. VD3 and LXR agonist (T0901317) combination demonstrated greater potency in inhibiting cholesterol accumulation and inducing apoptosis via ABCA1-CHOP-BCL-2 cascade in MCF-7 breast cancer cells Mol Biol Rep Oct 1 2020 [PMID: 32990902]
• Zhao D, Li J, Xue C et al. TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells J. Biol. Chem. Sep 22 2020 [PMID: 32963108] (Mouse)
• Xia J, Wang W, Xia X et al. Triptolide suppresses secretion of inflammatory factors and promotes expression of ABCA1 International Journal of Clinical and Experimental Medicine Jun 30 2019 (WB, Mouse)
• Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics Aug 13 2020 [PMID: 32929345] (WB, Mouse)
• Venkat P, Zacharek A, Landschoot-Ward J et al. Exosomes derived from bone marrow mesenchymal stem cells harvested from type two diabetes rats promotes neurorestorative effects after stroke in type two diabetes rats Experimental neurology Sep 2 2020 [PMID: 32889008] (IHC-P, Rat)
• Xia X, Xu Q, Liu M et al. Deubiquitination of CD36 by UCHL1 promotes foam cell formation Cell Death Dis Aug 15 2020 [PMID: 32801299] (IP)
• Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research Jul 1 2020 [PMID: 32621955] (WB, Mouse)
• Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. Jun 30 2020 [PMID: 32602556] (WB, Rat)
• Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. May 8 2020 [PMID: 32437644] (WB, Human)
• Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020 May 21 [PMID: 32437392] (WB, Human)
• Pathak P, Helsley RN, Brown AL et al. Small Molecule Inhibition of Gut Microbial Choline Trimethylamine Lyase Activity Alters Host Cholesterol and Bile Acid Metabolism Am. J. Physiol. Heart Circ. Physiol. Apr 24 2020 [PMID: 32330092] (WB, Mouse)
• Hiebl V, Schachner D, Ladurner A et al. Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations Biol Proced Online Apr 11 2020 [PMID: 32308567]
• Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. Mar 27 2020 [PMID: 32292343] (WB, Rat)
• He Q, Wang F, Honda T et al. Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation Sci Rep Apr 9 2020 [PMID: 32273567] (KO, Mouse)
• Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. Feb 28 2020 [PMID: 32173266] (WB, Mouse)
• Patel S THE ROLE OF MYELOID GSK3a/B IN ATHEROSCLEROSIS AND ATHEROSCLEROTIC REGRESSION Thesis Jan 1 2022 (IHC-P, Mouse)
• Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. May 4 2020 [PMID: 32097464] (Flow, Mouse)
• Cipollari, E, Szapary, H J Et al. Correlates and Predictors of Cerebrospinal Fluid Cholesterol Efflux Capacity from Neural Cells, a Family of Biomarkers for Cholesterol Epidemiology in Alzheimer's Disease. J Alzheimers Dis Feb 18 2020 [PMID: 32065798] (WB, Rat)
• Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy Jan 16 2020 [PMID: 31944172]
• Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. Dec 18 2019 [PMID: 31864712] (WB, Rat)
• Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. Nov 14 2019 [PMID: 31771811] (WB, Mouse)
• Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. Nov 25 2019 [PMID: 31769014]
• Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med Nov 21 2019 [PMID: 31766415] (WB, Human)
• Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. Nov 19 2019 [PMID: 31744820] (WB, Human, Mouse)
• Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019 Oct 27 [PMID: 31666189] (ICC/IF, WB, KD, Human, Mouse)
• Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules Oct 18 2019 [PMID: 31635211] (WB, Mouse)
• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
• Hennessy EJ, van Solingen C, Scacalossi KR et al. The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Nat Metab Jan 1 2019 [PMID: 31410392] (WB, Human)
• Zhou Y, Bazick H, Miles JR et al. A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic mice Exp. Neurol. Aug 3 2019 [PMID: 31386828] (WB, IHC-Fr, Mouse)
• Paul A, Lydic TA, Hogan R, Goo YH Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells Int J Mol Sci Aug 2 2019 [PMID: 31382484] (WB, Mouse)
• Mukhamedova N, Hoang A, Dragoljevic D et al. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog. Jul 1 2019 [PMID: 31344124] (ICC/IF, Human, Mouse)
• Koyama S, Horie T, Nishino T et al. Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice J Am Heart Assoc Jul 2 2019 [PMID: 31242815] (IHC-Fr, Mouse)
• Rege SD, Luiz R, Tsai B et al. Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame it on Fructose? Mol Nutr Food Res May 13 2019 [PMID: 31087499] (WB, Rat)
• Melendez-Avalos A, Sainz-Espunes T, Castrillon-Rivera LE et al. PMP22 regulates cholesterol trafficking and ABCA1-mediated cholesterol efflux J. Neurosci. May 6 2019 [PMID: 31061090] (WB, Mouse)
• Tiwari P, Blank A, Cui C, Schoenfelt KQ et al Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer. J Exp Med. 2019 Jun 3 [PMID: 31053611] (Flow, Human)
  
  Details:
  Citation using the DyLight 650 version of this antibody.
• Liao S, Zhou Q, Zhang Y. Elastic aortic wrap reduced aortic stiffness by partially alleviating the impairment of cholesterol efflux capacity in pigs J Diabetes Metab Disord Aug 7 2018 [PMID: 30918842] (WB, Porcine)
• Storti F, Klee K, Todorova V et al. Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration Elife Mar 13 2019 [PMID: 30864945] (WB, ICC/IF, Mouse)
• Margaryan NV, Hazard-Jenkins H, Salkeni MA et al. The Stem Cell Phenotype of Aggressive Breast Cancer Cells Cancers (Basel) Mar 8 2019 [PMID: 30857267] (IHC, Human)
• Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) Mar 1 2019 [PMID: 30821416] (B/N, Mouse)
• Nakazeki F, Tsuge I, Horie T et al. MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons Clin. Sci. Feb 28 2019 [PMID: 30777884] (WB, Human)
• Tang S, Tabet F, Cochran BJ et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle Sci Rep Feb 4 2019 [PMID: 30718702] (WB, Human)
• Vaidya M, Jentsch JA, Peters S et al. Regulation of ABCA1-mediated cholesterol efflux by sphingosine-1-phosphate signalling in macrophages. J. Lipid Res. Jan 17 2019 [PMID: 30655318] (WB, Mouse)
• Ramon-Vazquez A, de la Rosa JV, Tabraue C et al. Common and Differential Transcriptional Actions of Nuclear Receptors LXRa and LXRb in macrophages. Mol. Cell. Biol. Jan 2 2019 [PMID: 30602495] (WB, Mouse)
• Moon SH, Huang CH, Houlihan SL et al. p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression Cell Dec 14 2018 [PMID: 30580964] (WB, Human)
• Torres-Adorno AM, Vitrac H, Qi Y et al. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux. Oncogene. Nov 20 2018 [PMID: 30459358] (Human)
• Zinkhan EK, Yu B, McKnight R. Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet. Reprod Sci. Nov 19 2018 [PMID: 30453824] (Rat)
• Han Q, Yeung SC, Ip MSM, Mak JCW. Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model. Biochem. Pharmacol. Nov 9 2018 [PMID: 30428911] (WB, Rat)
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  Details:
  This publication used the DL488 conjugated form of this antibody (NB400-105G)
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• Hoe HS, Cooper MJ, Burns MP, Lewis PA, van der Brug M, Chakraborty G, Cartagena CM, Pak DT, Cookson MR, Rebeck GW. The metalloprotease inhibitor TIMP-3 regulates amyloid precursor protein and apolipoprotein E receptor proteolysis. J Neurosci;27(40):10895-905. 2007 Oct 3. [PMID: 17913923]
• Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008 Mar. [PMID: 17884990] (IHC, Rat)
• Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007 Dec. [PMID: 17823458] (WB, Chicken)
• Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation;116(12):1404-12. 2007 Sep 18. [PMID: 17724261]
• Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007 Nov. [PMID: 17656736]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007 Oct. [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007 Oct. [PMID: 17609524]
• Zelcer N, Khanlou N, Clare R et al. Attenuation of neuroinflammation and Alzheimer's disease pathology by liver x receptors. Proc Natl Acad Sci U S A 2007 Jun 19 [PMID: 17563384] (Mouse)
• Sporstol M, Mousavi SA, Eskild W et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol;8:5. 2007 Jan 22. [PMID: 17241464]
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006 [PMID: 16807312] (WB, Porcine)
• Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006 [PMID: 16537455] (IHC, Mouse)
• Dong J, Liu J, Lou B et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res;47(6):1307-14. 2006 Jun. [PMID: 16508036]
• Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006 [PMID: 16492740]
• Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH, Phillips MC. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res;47(4):832-43. 2006 Apr. [PMID: 16418537]
• Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006 Mar. [PMID: 16410462]
• Takata Y, Chu V, Collins AR et al. Transcriptional repression of ATP-binding cassette transporter A1 gene in macrophages: a novel atherosclerotic effect of angiotensin II. Circ Res 2005 Oct 28 [PMID: 16224068] (WB, Mouse)
• Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006 [PMID: 16077083]
• Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005 Aug. [PMID: 15961705] (WB, Human, Rat)
• Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005 [PMID: 15890646] (WB, Human)
• Ragozin S, Niemeier A, Laatsch A et al. Knockdown of hepatic ABCA1 by RNA interference decreases plasma HDL cholesterol levels and influences postprandial lipemia in mice. Arterioscler Thromb Vasc Biol 2005 Jul [PMID: 15845910]
• Makowski L, Brittingham KC, Reynolds JM et al. The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem 2005 Apr 1 [PMID: 15684432] (EMSA, Mouse)
• Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004 [PMID: 15578089]
• Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005 [PMID: 15557325] (WB, Mouse)
• Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005 Jan. [PMID: 15499044]
• O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation;110(18):2881-8. 2004 Nov 2. [PMID: 15492319]
• Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004 Dec. [PMID: 15469992]
• Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004 [PMID: 15358760] (WB, Human)
• Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004 [PMID: 15280376] (IP, Human)
• Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004 Oct. [PMID: 15210833]
• Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004 [PMID: 15148325] (WB, Human)
• Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004 Aug. [PMID: 15039140]
• Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004 [PMID: 14993246] (WB, Rat)
• Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004 [PMID: 14993242] (WB, Human)
• Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004 [PMID: 14754908] (IP, Human)
• Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004 Mar. [PMID: 14734645]
• Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004 [PMID: 14701824] (IP, Human)
• Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003 [PMID: 14673117] (ICC/IF, Mouse, Human)
• Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004 [PMID: 14660648] (IP, Human)
• Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003 [PMID: 14529830] (WB, Mouse)
• Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol;285(6):F1092-9. 2003 Dec. [PMID: 12952856]
• Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003 [PMID: 12909583] (WB, Human)
• Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003Aug. [PMID: 12897148] (WB, Mouse)
• Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003 [PMID: 12890677] (WB, Mouse)
• Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003 [PMID: 12777468] (WB, Mouse)
• Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003 [PMID: 12754274] (WB, Mouse)
• Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003 [PMID: 12754201] (WB, Mouse)
• Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003 [PMID: 12586329] (IHC-P, Mouse)
• Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003 [PMID: 12576511] (WB, Mouse)
• Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003 [PMID: 12547833] (WB, IHC, ICC/IF, Rat)
• Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003 [PMID: 12531903] (WB, Human, Hamster)
• Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003 [PMID: 12511593] (WB, IP, Human)
• Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003 Jan [PMID: 12507911] (WB, Mouse)
• Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002 [PMID: 12454270] (IP, Human)
• Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol;22(11):1877-83. 2002 Nov 1. [PMID: 12426219]
• Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002 [PMID: 12384498] (WB, Mouse, Human)
• Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002 [PMID: 12215451] (WB, Mouse)
• Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002 [PMID: 12091489] (WB, Human)
• Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002 [PMID: 12050168] (WB, Mouse, Human)

Reviews for ABCA1 Antibody (NB400-105) (7)

Average Rating: 4.3

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Marina Villar WB Human 08/15/2021

Summary

• Application: Western Blot
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: AM-5

Comments

• Comments: 10 ug of total protein. 7,5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4ºC. Secondary 1:5000 HRP 1h RT.

reviewed by: Verified Customer WB Mouse 11/16/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: AF-2

reviewed by: cynthia morimoto WB Mouse 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23519696
• Species: Mouse

reviewed by: Verified Customer IF Human 11/03/2014

Summary

• Application: Immunofluorescence
• Sample Tested: SK-N-SH human neuroblastoma cells
• Species: Human

reviewed by: Natalie FOURNIER WB Rat 10/15/2012

Summary

• Application: Western Blot
• Sample Tested: Cardiomyocytes from newborn rats
• Species: Rat

reviewed by: Verified Customer WB Mouse 09/27/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: System Admin WB Other 06/15/2009

Summary

• Application: Western Blot
• Sample Tested: Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
• Species: Other

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
   • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
2. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
   • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
3. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
   • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
4. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
   • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
5. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
   • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
6. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
   • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
7. Does this product contain any BSA?
   • No, this antibody does not have any BSA in its formulation.
8. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
   • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
9. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
   • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
10. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
    • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
11. Can i use DTT instead of Beta- mercaptoethanol?
    • Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
12. Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
    • We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.
Show All 12 FAQs.

Bioinformatics

• Gene Symbol: ABCA1
• Entrez:
  • 19(Human)
  • 11303(Mouse)
  • 313210(Rat)
• Uniprot:
  • O95477(Human)