ABCA1 Antibody

Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Cell Surface): ABCA1 Antibody [NB400-105] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). A surface stain was performed with ABCA1 Antibody NB400-105 (blue) and a matched isotype control NB810-56910 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody (SA5-10033, Thermo Fisher).

Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1, using NB400-105. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

• Reactivity: Hu, Mu, Rt, Po, Ca, Ch, Ha, Md
• Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, GS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ABCA1 Antibody Summary

• Immunogen: Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
• Localization: Astrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Simple Western 1:50
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Gel Supershift Assay
• Application Notes: This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). In Western blot a band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation and ELISA were also reported in scientific literature.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Charge.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raw 264.7 Whole Cell - T0901317 treated Kit (NBP1-30159)

• Control Peptide:

  ABCA1 Peptide (NB400-105PEP)

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA1 Antibody

• ABC1
• ABC-1
• ABC1ATP-binding cassette transporter A1
• ABCA1
• ATP-binding cassette 1
• ATP-binding cassette transporter 1
• ATP-binding cassette, sub-family A (ABC1), member 1
• CERP
• Cholesterol efflux regulatory protein
• EC 2.7.7.8
• EC 2.8.1.8
• EC 3.6.3
• EC 3.6.3.41
• FLJ14958
• HDLDT1
• membrane-bound
• MGC164864
• MGC165011
• TGD
• TGDATP-binding cassette sub-family A member 1

Background

ABCA1 (ATP-binding cassette transporter A1), ATP-binding cassette transporter family member, is a cAMP-dependent and sulfonylurea-sensitive anion transporter which is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux from macrophages and act as a key gatekeeper influencing intracellular cholesterol transport. ABCA1 is widely expressed with highest expression levels found in macrophages and is induced by bacterial LPS which regulates its expression through LXR (liver X receptor) -independent mechanism. ABCA1 interacts with MEGF10 and its expression is repressed by ZNF202. ABCA1 phosphorylation at Ser-2054 site regulates the phospholipid efflux and DHHC8 mediated palmitoylation of ABCA1 is essential for its membrane localization. ABCA1 expressed in brain neurons/glia mediates cholesterol transport to apoE which is the major CNS apolipoprotein, and it influences the motor function and synaptic morphology also. Defects in ABCA1 have been linked to HDLD1 (high density lipoprotein deficiency type 1; also known as analphalipoproteinemia, Tangier disease or TGD) and HDLD2 (high density lipoprotein deficiency type 2; also known as familial hypoalphalipoproteinemia or FHA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA1 Antibody (NB400-105)(277)

Publications using NB400-105

• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human)
• Zinkhan EK, Yu B, Schlegel A. Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1 Front Physiol May 29 2018 [PMID: 29896121] (WB, Rat)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-derived Macrophages Mol Nutr Food Res May 26 2018 [PMID: 29802792] (WB, Human)
• Feng T, Liu P, Wang X et al. SIRT1 activator E1231 protects from experimental atherosclerosis and lowers plasma cholesterol and triglycerides by enhancing ABCA1 expression Atherosclerosis Apr 30 2018 [PMID: 29787963] (WB, Mouse)
• Xie Z, Wang X, Liu X et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc. Mar 3 2018 [PMID: 29502100] (WB, Mouse)
• Casali BT, Reed-Geaghan EG, Landreth GE. Nuclear receptor agonist-driven modification of inflammation and amyloid pathology enhances and sustains cognitive improvements in a mouse model of Alzheimer's disease. J Neuroinflammation. 2018 Feb 15 [PMID: 29448961] (WB, Mouse)
• Sallam T, Jones M, Thomas BJ, Wu X. Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat. Med. 2018 Feb 12 [PMID: 29431742] (WB)
• Widenmaier SB, Snyder NA, Nguyen TB et al. NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis Cell. 2017 Nov 16 [PMID: 29149604] (WB)
• Doran AC, Ozcan L, Cai B et al. CAMKIIg suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J. Clin. Invest. 2017 Nov 01 [PMID: 28972541] (WB)
• Storti F, Raphael G, Griesser V et al. Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells Exp. Eye Res. 2017 Sep 21 [PMID: 28943268] (ICC/IF, Human)
• Liang Y, Li X, Zhang Y et al. Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells Attenuate Cigarette Smoke-Induced Cardiac Remodeling and Dysfunction Front Pharmacol 2017 Aug 14 [PMID: 28804458] (WB, Rat)
• Morizawa YM, Hirayama Y, Ohno N et al. Reactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway Nat Commun 2017 Jun 22 [PMID: 28642575] (IHC-FR, Mouse)
• Wang D, Tosevska A, Heib EH et al. Bilirubin Decreases Macrophage Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Protein Expression J Am Heart Assoc 2017 Apr 28 [PMID: 28455345] (WB, Rat)
• Li J, Xiu Z, Wang R et al. The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPARgamma. Life Sci. May 15 2017 [PMID: 28377266]
• Mailleux J, Vanmierlo T, Bogie JF et al. Active liver X receptor signaling in phagocytes in multiple sclerosis lesions. Mult. Scler. Feb 1 2017 [PMID: 28273782]
• Pulakazhi Venu VK, Adijiang A, Seibert T et al. Heat shock protein 27-derived atheroprotection involves reverse cholesterol transport that is dependent on GM-CSF to maintain ABCA1 and ABCG1 expression in ApoE(-/-) mice. FASEB J. 2017 Feb 23 [PMID: 28232480]
• Cook EC, Nelson JK, Sorrentino V et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017 Feb 23 [PMID: 28231341]
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017 Feb 16 [PMID: 28205585] (WB, Mouse)
• Hafiane A, Genest J. ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis Atherosclerosis Jan 17 2017 [PMID: 28129550] (WB, Human)
• Dong P, Pan L, Zhang X et al. Hawthorn (Crataegus pinnatifida Bunge) leave flavonoids attenuate atherosclerosis development in apoE knock-out mice J Ethnopharmacol Jan 21 2017 [PMID: 28119096] (WB, Mouse)
• Nishiga M, Horie T, Kuwabara Y et al. MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol. Circ. Res. Dec 5 2016 [PMID: 27920122] (Human)
• Cermak S, Kosicek M, Mladenovic-Djordjevic A et al. Loss of Cathepsin B and L Leads to Lysosomal Dysfunction, NPC-Like Cholesterol Sequestration and Accumulation of the Key Alzheimer's Proteins. PLoS ONE. Nov 30 2016 [PMID: 27902765] (WB, Chinese Hamster)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)
• Luo L, Yang T. Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med. Oct 1 2016 [PMID: 27698729] (Mouse)
• Conlon Dm, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J. Clin. Invest. Sep 6 2016 [PMID: 27599291] (IB)
• de Gaetano M, Crean D, Barry M, Belton O. M1- and M2-Type Macrophage Responses Are Predictive of Adverse Outcomes in Human Atherosclerosis Front Immunol 2016 Aug 03 [PMID: 27486460] (WB)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Cui C, Ye X, Chopp M et al. miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats. Stem Cells Transl Med. Jul 26 2016 [PMID: 27460851] (IHC, Rat)
• Bechor S, Zolberg Relevy N, Harari A et al. 9-cis beta-Carotene Increased Cholesterol Efflux to HDL in Macrophages. Nutrients 2016 Jul 19 [PMID: 27447665] (WB)
• Khan F, Ahmed F, Pushparaj PN et al. Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest PLoS ONE 2016 Jul 22 [PMID: 27441372] (WB, Human)
• Kim SY, Lim EJ, Yoon YS et al. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages Sci Rep 2016 Jul 13 [PMID: 27406916]
• Zinkhan EK, Zalla JM, Carpenter JR et al. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats Physiol Rep 2016 Jul 01 [PMID: 27401460]
• Hnatiuk AP, Ong SG, Olea FD et al. Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-a (HIF1-a) in an Ovine Model of Acute Myocardial Infarction J Am Heart Assoc 2016 Jul 01 [PMID: 27385426] (WB, Mouse)
• Hsieh J, Koseki M, Molusky MM et al. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis Nature 2016 Jul 14 [PMID: 27383786]
• Kemmerer M, Wittig I, Richter F et al. AMPK activates LXR alpha and ABCA1 expression in human macrophages. Int. J. Biochem. Cell Biol. Jun 22 2016 [PMID: 27343431] (WB)
• Ollikainen E, Tulamo R, Lehti S et al. Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture. J. Neuropathol. Exp. Neurol. Jul 1 2016 [PMID: 27283327] (ICC/IF)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID: 27063951] (WB, Mouse)
• Wolfe AR, Bambhroliya A, Reddy JP et al. MiR-33a Decreases High-Density Lipoprotein-Induced Radiation Sensitivity in Breast Cancer. Int. J. Radiat. Oncol. Biol. Phys. Jun 1 2016 [PMID: 27055396] (WB, Human)
• Shrestha E, Hussein MA, Savas JN et al. PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J. Biol. Chem. 2016 Mar 29 [PMID: 27026705] (WB, Mouse)
• Hong L, Xu X, Huang J et al. Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency Sci Rep Feb 8 2016 [PMID: 26852751] (IHC-P, Porcine)
• Schafer-Somi S, Palme N. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability Reprod. Domest. Anim. Feb 5 2016 [PMID: 26848092] (ICC/IF, Canine)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)
• Fournier N, Tardivel S, Benoist JF et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim. Biophys. Acta. 2016 Jan 08 [PMID: 26776055] (WB, Human, Mouse)
• Merkl M, Ertl R, Handschuh S et al. The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues. Theriogenology 2016 Apr 1 [PMID: 26711702]
• Schreurs BG, Sparks DL. Dietary high cholesterol and trace metals in the drinking water increase levels of ABCA1 in the rabbit hippocampus and temporal cortex. J Alzheimers Dis 2015 [PMID: 26444796] (IHC, Rabbit)
• Hussein MA, Shrestha E, Ouimet M et al. LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2. PLoS ONE. 2015 Aug 20 [PMID: 26288135] (WB, Mouse)
• Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes 2015 Dec [PMID: 26253613]
• Ito A, Hong C, Rong X et al. LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling Elife. 2015 Jul 30 [PMID: 26173179] (WB, Mouse)
• Ramezani A, Dubrovsky L, Pushkarsky T et al. Stimulation of Liver X Receptor Has Potent Anti-HIV Effects in a Humanized Mouse Model of HIV Infection J. Pharmacol. Exp. Ther. 2015 Sep 01 [PMID: 26126533] (WB, Human)
• Casali BT, Corona AW, Mariani MM et al. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease J. Neurosci. 2015 Jun 17 [PMID: 26085639] (WB, Mouse)
• Fond AM, Lee CS, Schulman IG et al. Apoptotic cells trigger a membrane-initiated pathway to increase ABCA1. J Clin Invest 2015 Jul 1 [PMID: 26075824] (WB)
• Baker AH, Watt J, Huang CK et al. Tributyltin Engages Multiple Nuclear Receptor Pathways and Suppresses Osteogenesis in Bone Marrow Multipotent Stromal Cells. Chem. Res. Toxicol. 2015 May 13 [PMID: 25932594] (WB, Mouse)
• Liu Q, Li J, Liang Q et al. Sparstolonin B suppresses rat vascular smooth muscle cell proliferation, migration, inflammatory response and lipid accumulation Vascul. Pharmacol. 2015 Apr 11 [PMID: 25869499] (WB, Rat)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015 Feb 21 [PMID: 25708389] (WB, Mouse)
• Kuo Cheng-Hsiang, Leon Lorraine, Chung Eun Ji et al. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B Mater Biol Med. 2014 Dec 14 [PMID: 25685357]
• Park SH, Paek JH, Shin D et al. Purple perilla extracts with alpha-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages Int. J. Mol. Med. 2015 Feb 12 [PMID: 25673178] (WB, Mouse)
• Chou JL, Huang RL, Shay J et al. Hypermethylation of the TGF-beta target, ABCA1 is associated with poor prognosis in ovarian cancer patients. Clin Epigenetics 2015 [PMID: 25628764] (IHC)
• Hong C, Marshall Sm, Mcdaniel Al et al. The LXR-Idol Axis Differentially Regulates Plasma LDL Levels in Primates and Mice. Cell Metab. 2014 Nov 04 [PMID: 25440061]
• Dong F, Mo Z, Eid W et al. Akt Inhibition Promotes ABCA1-Mediated Cholesterol Efflux to ApoA-I through Suppressing mTORC1. PLoS OnE. 2014 Nov 22 [PMID: 25415591] (WB, Hamster)
• Shibuya Y, Chang Cc, Huang Lh et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases AB1-42 Clearance. J. neurosci. 2014 Oct 22 [PMID: 25339759] (WB, Mouse)
• Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014 Sep 12 [PMID: 25217640]
• Ahn Sang Bong, Jang Kiseok, Jun Dae Won et al. Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease. Dig Dis Sci. 2014 Aug 08 [PMID: 25102981] (Human)
• Horie Takahiro, Nishino Tomohiro, Baba Osamu et al. MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. Sci Rep. 2014 Jun 16 [PMID: 24931346] (Mouse)
• Xu Y, Liu Q, Xu Y et al. Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT. J Lipid Res. 2014 Jun 7 [PMID: 24908654]
• Chi Yan, Wang Le, Liu Yuanyuan et al. 7-ketocholesteryl-9-carboxynonanoate enhances ATP binding cassette transporter A1 expression mediated by PPARgamma in THP-1 macrophages. Atherosclerosis. 2014 Feb 15 [PMID: 24814410] (Human)
• Liu W, Jiang J, Yan D et al. Pentraxin 3 promotes oxLDL uptake and inhibits cholesterol efflux from macrophage-derived foam cells. Exp. Mol. Pathol. 3/24/2014 [PMID: 24675235] (WB, Human)
• Sallam T, Ito A, Rong X et al. The macrophage lipopolysaccharide binding protein gene is an LXR target that promotes macrophage survival and atherosclerosis. J. Lipid Res. 3/26/2014 [PMID: 24671012] (WB, Mouse)
• Bocchetta S, Maillard P, Yamamoto M et al. Up-Regulation of the ATP-Binding Cassette Transporter A1 Inhibits Hepatitis C Virus Infection. PLoS ONE 3/20/2014 [PMID: 24646941] (WB, Human)
• Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 3/5/2014 [PMID: 24637412] (WB, Mouse)
• Palme N, Becher A, Merkl M et al. Immunolocalization of the Cholesterol Transporters ABCA1 and ABCG1 in Canine Reproductive Tract Tissues and Spermatozoa. Reprod. Domest. Anim. 3/11/2014 [PMID: 24612239] (IHC-P, ICC/IF, Canine)
• Vinod M, Chennamsetty I, Colin S et al. miR-206 controls LXRa expression and promotes LXR-mediated cholesterol efflux in macrophages. Biochim. Biophys. Acta 3/3/2014 [PMID: 24603323] (WB, Human, Mouse)
• Allahverdian S, Chehroudi AC, McManus BM, Francis GA. Contribution of Intimal Smooth Muscle Cells to Cholesterol Accumulation and Macrophage-Like Cells in Human Atherosclerosis. Circulation 2014 Jan 31 [PMID: 24481950] (IHC-P, Human)
• Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013 Dec 4 [PMID: 24371802] (WB, Human)
• Aseem O, Smith BT, Cooley MA et al. Cubilin Maintains Blood Levels of HDL and Albumin. J Am Soc Nephrol 2013 Dec 19 [PMID: 24357674]
• Li N, Xu Y, Feng T et al. Identification of a Selective Agonist for Liver X Receptor alpha (LXRalpha) via Screening of a Synthetic Compound Library. J Biomol Screen 2013 Dec 13 [PMID: 24334278] (WB, Mouse, Human)
• Ying R, Yuan Y, Qin YF. The combination of L-4F and simvastatin stimulate cholesterol efflux and related proteins expressions to reduce atherosclerotic lesions in apoE knockout mice. Lipids Health Dis 2013 Dec 8 [PMID: 24314261] (WB, Mouse)
• Horie T, Nishino T, Baba O et al. MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice. Nat Commun. 2013 Dec 3 [PMID: 24300912] (WB, Human, Mouse)
• Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013 Nov 6 [PMID: 24223214] (WB, Mouse)
• Ingram WJ, Crowther LM, Little EB et al. ABC transporter activity linked to radiation resistance and molecular subtype in pediatric medulloblastoma. Exp Hematol Oncol 2013 Oct 4 [PMID: 24219920] (ICC/IF, Human)
  
  Details:
  This publication used the DL488 conjugated form of this antibody (NB400-105G)
• Ohara K, Wakabayashi H, Taniguchi Y et al. Quercetin-3-O-glucuronide induces ABCA1 expression by LXRalpha activation in murine macrophages. Biochem Biophys Res Commun. 2013 Nov 29 [PMID: 24216107] (WB, Mouse)
• Baumann M, Korner M, Huang X et al. Placental ABCA1 and ABCG1 expression in gestational disease: Pre-eclampsia affects ABCA1 levels in syncytiotrophoblasts. Placenta 2013 Jul 20 [PMID: 23880356] (WB, Human)
• Hu Y, Yang Y, Yu Y et al. Synthesis and Identification of New Flavonoids Targeting Liver X Receptor beta Involved Pathway as Potential Facilitators of Abeta Clearance with Reduced Lipid Accumulation. J Med Chem 2013 Aug 8 [PMID: 23844653] (WB, Mouse)
• Shao F, Ford DA. Differential Regulation of ABCA1 and Macrophage Cholesterol Efflux by Elaidic and Oleic Acids. Lipids 2013 Jun 26 [PMID: 23800855] (WB, Human)
• A-Gonzalez N, Guillen JA, Gallardo G et al. The nuclear receptor LXRalpha controls the functional specialization of splenic macrophages. Nat Immunol 2013 Aug [PMID: 23770640] (WB, Human, Mouse)
• Pommier AJ, Dufour J, Alves G et al. Liver X Receptors Protect from Development of Prostatic Intra-Epithelial Neoplasia in Mice. PLoS Genet 2013 May [PMID: 23675307] (WB, Mouse)
• Lyssenko NN, Nickel M, Tang C, Phillips MC. Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability. FASEB J 2013 Jul [PMID: 23543682] (Human)
• de Aguiar Vallim TQ, Tarling EJ, Kim T et al. MicroRNA-144 regulates hepatic ATP binding cassette transporter A1 and plasma high-density lipoprotein after activation of the nuclear receptor farnesoid X receptor Circ Res. 2013 Jun 7 [PMID: 23519696] (WB, Mouse)
• Scheffler K, Krohn M, Dunkelmann T, Stenzel J, Miroux B, Ibrahim S, von Bohlen Und Halbach O, Heinze HJ, Walker LC, Gsponer JA, Pahnke J. Mitochondrial DNA polymorphisms specifically modify cerebral ?-amyloid proteostasis. Acta Neuropathol. 2012 Apr 18. [PMID: 22526016] (WB, Mouse)
• Castilho G, Okuda LS, Pinto RS, Iborra RT, Nakandakare ER, Santos CX, Laurindo FR, Passarelli M. ER stress is associated with reduced ABCA-1 protein levels in macrophages treated with advanced glycated albumin - Reversal by a chemical chaperone. Int J Biochem Cell Biol. 2012 Apr 3. [PMID: 22497927] (ICC/IF, Mouse)
• Iio A, Ohguchi K, Iinuma M, Nozawa Y, Ito M. Hesperetin Upregulates ABCA1 Expression and Promotes Cholesterol Efflux from THP-1 Macrophages. J Nat Prod;75(4):563-6. 2012 Apr 27. [PMID: 22429094] (WB, Human)
• Cui HL, Grant A, Mukhamedova N et al. HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux. Journal of Lipid Research. 2012 Jan 19. [PMID: 22262807]
• McIntosh AL, Atshaves BP, Storey SM et al. Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. Journal of Lipid Research. 2012 Jan 5. [PMID: 22223861]
• Boadu E, Nelson R, Francis GA. ABCA1-dependent mobilization of lysosomal cholesterol requires functional Niemann-Pick C2 but not Niemann-Pick C1 protein. Biochimica Et Biophysica Acta. 2011 Dec 10. [PMID: 22179027]
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• Ni S, Ma L, Denis M et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res;50(3):456-66. 2009 Mar. [PMID: 18941142] (ICC/IF, Hamster)
• Pou J, Rebollo A, Roglans N, Sanchez RM, Vazquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008 Dec. [PMID: 18849545]
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• Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol;295(1):C242-8. 2008 Jul. [PMID: 18508910]
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• Rode S, Rubic T, Lorenz RL et al. alpha-Tocopherol disturbs macrophage LXRalpha regulation of ABCA1/G1 and cholesterol handling. Biochem Biophys Res Commun 2008 May 9 [PMID: 18328805] (WB, Human)
• Henry-Berger J, Mouzat K, Baron S, Bernabeu C, Marceau G, Saru JP, Sapin V, Lobaccaro JM, Caira F. Endoglin (CD105) expression is regulated by the liver X receptor alpha (NR1H3) in human trophoblast cell line JAR. Biol Reprod;78(6):968-75. 2008 Jun. [PMID: 18276933]
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• Wang N, Yvan-Charvet L, Lutjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J;22(4):1073-82. 2008 Apr. [PMID: 18039927]
• Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008 Mar. [PMID: 17884990] (IHC, Rat)
• Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007 Dec. [PMID: 17823458] (WB, Chicken)
• Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007 Nov. [PMID: 17656736]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007 Oct. [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007 Oct. [PMID: 17609524]
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006 [PMID: 16807312] (WB, Porcine)
• Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006 [PMID: 16537455] (IHC, Mouse)
• Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006 [PMID: 16492740]
• Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH, Phillips MC. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res;47(4):832-43. 2006 Apr. [PMID: 16418537]
• Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006 Mar. [PMID: 16410462]
• Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006 [PMID: 16077083]
• Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005 Aug. [PMID: 15961705] (WB, Human, Rat)
• Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005 [PMID: 15890646] (WB, Human)
• Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004 [PMID: 15578089]
• Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005 [PMID: 15557325] (WB, Mouse)
• Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005 Jan. [PMID: 15499044]
• Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004 Dec. [PMID: 15469992]
• Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004 [PMID: 15358760] (WB, Human)
• Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004 [PMID: 15280376] (IP, Human)
• Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004 Oct. [PMID: 15210833]
• Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004 [PMID: 15148325] (WB, Human)
• Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004 Aug. [PMID: 15039140]
• Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004 [PMID: 14993246] (WB, Rat)
• Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004 [PMID: 14993242] (WB, Human)
• Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004 [PMID: 14754908] (IP, Human)
• Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004 Mar. [PMID: 14734645]
• Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004 [PMID: 14701824] (IP, Human)
• Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003 [PMID: 14673117] (ICC/IF, Mouse, Human)
• Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004 [PMID: 14660648] (IP, Human)
• Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003 [PMID: 14529830] (WB, Mouse)
• Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol;285(6):F1092-9. 2003 Dec. [PMID: 12952856]
• Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003 [PMID: 12909583] (WB, Human)
• Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003Aug. [PMID: 12897148] (WB, Mouse)
• Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003 [PMID: 12890677] (WB, Mouse)
• Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003 [PMID: 12777468] (WB, Mouse)
• Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003 [PMID: 12754274] (WB, Mouse)
• Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003 [PMID: 12754201] (WB, Mouse)
• Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003 [PMID: 12586329] (IHC-P, Mouse)
• Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003 [PMID: 12576511] (WB, Mouse)
• Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003 [PMID: 12547833] (WB, IHC, ICC/IF, Rat)
• Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003 [PMID: 12531903] (WB, Human, Hamster)
• Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003 [PMID: 12511593] (WB, IP, Human)
• Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002 [PMID: 12454270] (IP, Human)
• Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002 [PMID: 12384498] (WB, Mouse, Human)
• Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002 [PMID: 12215451] (WB, Mouse)
• Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002 [PMID: 12091489] (WB, Human)
• Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002 [PMID: 12050168] (WB, Mouse, Human)

Reviews for ABCA1 Antibody (NB400-105) (6)

Average Rating: 4.3

(Based on 6 reviews)

We have 6 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Jahangir Iqbal WB Mouse 11/16/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: AF-2

Blocking

• Blocking Details: Membranes were blocked in 5% BSA in TBST for 1 h at room temperature

Primary Anitbody

• Dilution Ratio: Membranes were immunoblotted with anti-ABCA1 antibody 1:2000 dilution in 2.5% BSA in TBST overnight at 4 oC

Secondary Antibody

• Secondary Description: Membranes were incubated with secondary antibody conjugated to stabilized peroxidase for 1 hour at room temperature
• Secondary Manufacturer Cat#: Thermo Scientific # 32460
• Secondary Concentration: dilution 1:15,000

Details

• Detection Notes: Bands were visualized used chemiluminescence according to the manufacturer’s instructions

Comments

• Comments: Samples were not boiled and 2X BME was added.

reviewed by: Anonymous WB Mouse 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23519696
• Species: Mouse

Blocking

• Blocking Details: See PMID 23519696

Details

• Detection Notes: See PMID 23519696

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23519696

reviewed by: Angeliki Chroni IF Human 11/03/2014

Summary

• Application: Immunofluorescence
• Sample Tested: SK-N-SH human neuroblastoma cells
• Species: Human

reviewed by: Anonymous WB Rat 10/15/2012

Summary

• Application: Western Blot
• Sample Tested: Cardiomyocytes from newborn rats
• Species: Rat

Blocking

• Blocking Details: PBS Milk 5%, 1 hour, room temperature

Primary Anitbody

• Dilution Ratio: 1000 = dilution, 1 hour, room temperature, PBS without tween and 2% Milk

Secondary Antibody

• Secondary Description: goat anti rabbit IgG HRP
• Secondary Manufacturer Cat#: Amersham NA934V
• Secondary Concentration: 3000 diluted

Details

• Detection Notes: ECL super plus Amersham RPN2132

reviewed by: Cynthia Hong WB Mouse 09/27/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Anonymous WB Other 06/15/2009

Summary

• Application: Western Blot
• Sample Tested: Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk w/ TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1/1000, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: anti-Rabbit IgG HRP, Secondary Ab Dilution Ratio: 1/1000

Details

• Detection Notes: Exposure Time: 1 hour, Positive Control: HC/RA, Specific Bands: 200 kDa

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 11 FAQs).

1. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
   • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
2. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
   • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
3. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
   • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
4. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
   • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
5. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
   • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
6. Does this product contain any BSA?
   • No, this antibody does not have any BSA in its formulation.
7. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
   • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
8. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
   • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
9. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
   • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
10. Can i use DTT instead of Beta- mercaptoethanol?
    • Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
11. Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
    • We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.
Show All 11 FAQs.

Bioinformatics

• Gene Symbol: ABCA1
• Entrez:
  • 19(Human)
  • 11303(Mouse)
  • 313210(Rat)
• Uniprot:
  • O95477(Human)