ABCA1 Antibody - BSA Free

Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

HSKMCs were transfected with scrambled siRNA (scr siRNA) or ABCA1 siRNA. The cells were lysed and ABCA1 (A) protein levels were quantified by immunoblotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-38014-3) licensed under under a CC-BY license.

Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. Representative image of ovarian cancer showing high (left panel) and low (right panel) ABCA1 expression on the cell membrane or cytoplasm (x400). Image collected and cropped by CiteAb from the following publication (//www.clinicalepigeneticsjournal.com/content/7/1/1), licensed under a CC-BY license.

ABCA1 in human primary fibroblasts in culture. 10 ug of total protein. 7.5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4C. Secondary 1:5000 HRP 1h RT. Arrow shows around 250 kDa. WB image submitted by a verified customer review.

ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high cholesterol diet were analyzed by western blotting with antibodies against ABCA1, LDLR and ACTIN as a loading control (left panel), quantification of ABCA1 and LDLR protein accumulation levels (right panel). * p<0.05, *** p<0.001 in Student's t test. Error bars represent the +/- mean SEM. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003483), licensed under a CC-BY license.

miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ mice. Representative images are shown. beta-actin was used as the loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY license.

miR-33b regulates ABCA1 and SREBP-1. Analysis for ABCA1, SREBP-1, CPT1a, and AMPK alpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are shown. TF2B and beta-actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY license.

Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

Liver mRNA & protein expression analysis of chow-fed Osbpl8KO mice.A: qPCR analysis of the quantity of the mRNAs identified at the bottom in chow-fed KO females (open bars) & males (closed bars). The mRNAs were quantified using ribosomal protein 36B4 message as a housekeeping reference. The data are expressed relative to quantity in littermate WT animals of the same gender, & represent mean ± s.e.m. (n = 6; *p<0.05, **p<0.01, T-test). B: Western blot analysis of ABCA1 & SR-B1 proteins in WT & KO mouse liver. The blots were probed with anti-beta -actin as a loading control. Densitometric quantification of the Western blot data is shown on the right. The results were normalized against beta -actin. The data represents mean ± s.e.m. (n = 4). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058856), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Protein expressions of ABCA1, SR-BI & ABCG1 in liver & macrophage by Western blot. Simvastatin increased the expressions of ABCA1 & ABCG1 in liver & ABCA1 in macrophage, Both L-4F & the combination group improved the expressions of ABCA1, SR-BI & ABCG1 in liver & ABCA1 & ABCG1 in macrophage. 1P < 0.05, 2P < 0.001, vs. AS group; aP < 0.05, bP < 0.001, vs. Simva group;*P < 0.05, **P < 0.001, vs. L-4F group. Image collected & cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-180), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ABCA1, ABCG1 & SR-BI protein expressions. a & b Hepatic protein expressions of ABCA1, ABCG1 & SR-BI were significantly decreased in COMT−/− mice at GD 18.5, compared to C57BL/6 J mice. Decreased hepatic ABCA1 expression was also observed at 10 days postpartum. ATI-5261 increased ABCA1 & ABCG1 expression in the liver at 10 days postpartum. c Placental protein expressions of ABCA1 & ABCG1 was reduced in COMT−/− mice, compared to C57BL/6 J mice. ATI-5261 treatment significantly increased ABCA1 levels in the placenta of COMT−/− mice. d Representative immunoblots of the corresponding proteins in the placenta with mouse RAW264.7 cell lysate included as positive control. Similar results were obtained when the experiment was repeated with lysates prepared from three batches of tissues. Data are presented as mean ± SEM. Groups (n = 8 in all groups) were compared using one-way ANOVA with post-hoc analysis (Tukey’s procedure). *, p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30237900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of 13-HODE & LA in the presence & absence of PPAR alpha & PPAR gamma selective antagonists on molecular markers of cholesterol homeostasis in RAW264.7 macrophages. RAW264.7 cells were pre-treated without or with the PPAR alpha selective antagonist GW6471 or the PPAR gamma selective antagonist GW9662 & subsequently treated without (vehicle control) or with 2.5 μmol/L 13-HODE or 100 μmol/L LA for 24 h. Afterwards, cells were lysed & subsequently processed for western blotting as described in the materials & methods section. A, Representative immunoblots specific for ABCA1, ABCG1, SR-BI, LXR alpha , & beta -actin which was used for normalization are shown. B, Bars represent data from densitometric analysis & are means ± SD from three independent experiments (n = 3). Data are expressed as percentage of protein concentration of vehicle control cells. Results from statistical analysis are indicated: Significant effects are denoted with superscript letters. Bars marked without a common superscript letter significantly differ (P < 0.05). Image collected & cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-222), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Protein expressions of ABCA1, SR-BI & ABCG1 in liver & macrophage by Western blot. Simvastatin increased the expressions of ABCA1 & ABCG1 in liver & ABCA1 in macrophage, Both L-4F & the combination group improved the expressions of ABCA1, SR-BI & ABCG1 in liver & ABCA1 & ABCG1 in macrophage. 1P < 0.05, 2P < 0.001, vs. AS group; aP < 0.05, bP < 0.001, vs. Simva group;*P < 0.05, **P < 0.001, vs. L-4F group. Image collected & cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-180), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Upregulation of ABCA1 & ABCG1 & (B) enhancement of cholesterol efflux by purple Perilla frutescens extracts (PPE) in 50 μg/ml Cu2+-oxidized low-density lipoproteins (LDL)-exposed J774A.1 murine macrophages. (A) For the measurement of ABCA1 & ABCG1 expression, total cell lysates were subjected to western blot analysis with a primary antibody against ABCA1 or ABCG1. beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. (B) Cholesterol efflux was expressed as the percentage of fluorescence in the medium relative to the total fluorescence. Bar graphs denoted without a common letter indicate significant difference, P<0.05. Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1, but not C) SR‐BI protein levels in cholesterol‐loaded THP‐1‐derived macrophages. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with increasing concentrations of 6‐dihydroparadol (1–30 μm) for another 24 h. The protein levels of ABCA1, ABCG1, & SR‐B1 were detected by Western blot analysis. The control was treated with solvent vehicle (0.1% DMSO). As a positive control, TO901317 (5 μm, 24 h) was used. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition suppresses mTORC1 activity.BHK-ABCA1 cells were induced overnight with mifepristone (10 nM) & then incubated with DEBC, LY294002 or rapamycin at indicated concentrations for 2 h. Cells were then lysed & analyzed for ABCA1 & phosphorylated S6K by Western blotting. Actin was also blotted as loading control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113789), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Reversal of hepatic steatosis by reduction of SREBP-1 levels.(a) Western blotting analysis of SREBP-1 & ABCA1 levels in livers of miR-33+/+Srebf1+/+, miR-33+/+Srebf1+/−, miR-33−/−Srebf1+/+ & miR-33−/−Srebf1+/− mice. Representative western blot images are shown (n=4). Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/24300912), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1 protein stability. THP‐1 cells were differentiated as described in Figure 2. Then cells were loaded with unlabeled cholesterol & treated with 6‐dihydroparadol (10 μm), or solvent vehicle (0.1% DMSO, control) for 24 h. Cells were lysed at different time points (0, 1, 2, 3, 4, & 6 h) after treatment with the protein synthesis inhibitor cycloheximide (CHX, 100 μm). The protein levels of both ABCA1 & ABCG1 were detected by Western blot analysis. The data points represent mean ± SD from three independent experiments. *p < 0.05 & **p < 0.01 versus control at the same time point (determined by Student's t‐test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Ligand activation of LXR inhibits LPS-induced MAP kinase activation through Abca1 induction.(A–D) Bone marrow-derived macrophages from Lxr alpha −/−Lxr beta −/− & control wild-type mice (A, B), or bone marrow-derived macrophages from myeloid-specific Abca1−/− & control wild-type mice (C, D) were pretreated with GW3965 (1 µM) overnight, followed by stimulation with LPS (10 ng/ml) for 20 min or 1 hr. Whole cell lysates were harvested & protein expression was analyzed by immunoblotting with the indicated antibodies (A, C). Protein expression was quantified by Image Quant TL7.0 (B, D). N = 4–6 per group. *p < 0.05, **p < 0.01, NS, not significant. Error bars represent means ± SEM.DOI://dx.doi.org/10.7554/eLife.08009.012 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/08009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1, but not C) SR‐BI protein levels in cholesterol‐loaded THP‐1‐derived macrophages. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with increasing concentrations of 6‐dihydroparadol (1–30 μm) for another 24 h. The protein levels of ABCA1, ABCG1, & SR‐B1 were detected by Western blot analysis. The control was treated with solvent vehicle (0.1% DMSO). As a positive control, TO901317 (5 μm, 24 h) was used. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Western Blotting for ABCA1, as detailed in Supplementary Protocol #5 (Additional file 1). Caco-2 cells cultivated on filter inserts were treated for 48 h with bexarotene (RXR agonist, 5 μM), T0901317 (LXR agonist, 10 μM), GW3965 (LXR agonist, 10 μM) or the vehicle control (DMSO 0.1%). Protein expression was then determined by western blot analysis. Results were normalized for interexperimental variance & then set in relation to the vehicle control. Bar graphs represent mean ± SD from three independent experiments. A representative blot is shown - the bands stem from the same membrane & the vertical line indicates excised irrelevant bands. ns not significant, *P < 0.05, **P < 0.01 versus solvent vehicle (determined by paired t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32308567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Ligand activation of LXR inhibits LPS-induced MAP kinase activation through Abca1 induction.(A–D) Bone marrow-derived macrophages from Lxr alpha −/−Lxr beta −/− & control wild-type mice (A, B), or bone marrow-derived macrophages from myeloid-specific Abca1−/− & control wild-type mice (C, D) were pretreated with GW3965 (1 µM) overnight, followed by stimulation with LPS (10 ng/ml) for 20 min or 1 hr. Whole cell lysates were harvested & protein expression was analyzed by immunoblotting with the indicated antibodies (A, C). Protein expression was quantified by Image Quant TL7.0 (B, D). N = 4–6 per group. *p < 0.05, **p < 0.01, NS, not significant. Error bars represent means ± SEM.DOI://dx.doi.org/10.7554/eLife.08009.012 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/08009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Ligand activation of LXR inhibits LPS-induced MAP kinase activation through Abca1 induction.(A–D) Bone marrow-derived macrophages from Lxr alpha −/−Lxr beta −/− & control wild-type mice (A, B), or bone marrow-derived macrophages from myeloid-specific Abca1−/− & control wild-type mice (C, D) were pretreated with GW3965 (1 µM) overnight, followed by stimulation with LPS (10 ng/ml) for 20 min or 1 hr. Whole cell lysates were harvested & protein expression was analyzed by immunoblotting with the indicated antibodies (A, C). Protein expression was quantified by Image Quant TL7.0 (B, D). N = 4–6 per group. *p < 0.05, **p < 0.01, NS, not significant. Error bars represent means ± SEM.DOI://dx.doi.org/10.7554/eLife.08009.012 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/08009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Expression of ABCA1 & CROT in livers & RIP140 in macrophages is elevated in miR-33−/−Apoe−/− mice compared with miR-33+/+Apoe−/− mice. A, Quantitative real-time PCR analysis of Abca1, Crot, Cpt1a, & Prkaa1in livers from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values from miR-33+/+Apoe−/− mice were set at 100%. Values are mean±SE (n=9 to 11each); **P<0.01. B, Western analysis of ABCA1, CROT, CPT1a, & AMPK alpha in livers from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. beta -actin was used as a loading control. C, Densitometry of ABCA1, CROT, CPT1a, & AMPK alpha in livers from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values from miR-33+/+Apoe−/− mice were set at 100%. Values are mean±SE (n=4 each); *P<0.05. D, Total cholesterol, free cholesterol, cholesterol ester, & triglyceride levels in livers of miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values are mean±SE (n=9 to 11 each). E, HE staining of livers of miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice at age 20 weeks fed NC. Scale bar: 100 μm. F, Quantitative real-time PCR analysis of Nrip1 (RIP140) in peritoneal macrophages from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values from miR-33+/+Apoe−/− mice were set at 100%. Values are mean±SE (n=7 each). G, Western analysis of NRIP1 (RIP140) in peritoneal macrophages from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. GAPDH was used as a loading control. H, Densitometry of NRIP1 (RIP140) in peritoneal macrophages from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values from miR-33+/+Apoe−/− mice were set at 100%. Values are mean±SE (n=4 each); *P<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23316322), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Basal ABCA1 expression & function in patient-derived iRPEs. (A) Representation of patient-derived iPSC lines & genotypes harboring polymorphisms in ABCA1 that are associated with decreased or increased risk for AMD development. (B) Relative expression of ABCA1 mRNA in patient-derived iRPEs under basal conditions normalized to RPL28 & decreased risk group. (C) Western blot analysis of ABCA1 levels in patient-derived iRPEs under basal conditions. Actin was detected as loading control. ABCA1 expression was quantified & normalized to actin. (D,E) Relative expression of ABCG1 (D) & NR1H3 (E) mRNA in patient-derived iRPEs under basal conditions normalized to RPL28 & decreased risk group. (F) Cholesterol efflux in patient-derived iRPEs after direct cell labeling & in the presence of ApoAI. (G) Cholesterol efflux in patient-derived iRPEs after phagocytosis of BODIPY-cholesterol-loaded POSs & in the presence of ApoAI. (H) Quantification of phagocytosed POSs per nuclei in patient-derived iRPEs. Values shown are means ± SD (n = 3). * p < 0.05; *** p < 0.001. Unpaired Student’s t-test. (I) Representative fluorescence microscopy images of phagocytosed FITC-labeled POSs (green) & staining for ZO-1 (red) & DAPI (blue) of increased risk cell line IPS19-00096. Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328615), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - A–C) Effects of 6‐dihydroparadol & specific proteasomal, lysosomal, & calpain inhibitors on the ABCA1 protein levels. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with or without 6‐dihydroparadol (6‐DP, 30 μm) for 24 h & incubated for another 3 h with or without the proteasome inhibitor lactacystin (10 μm), the lysosomal inhibitor chloroquine (100 μm), or the calpain inhibitor calpeptin (30 μg mL−1). The ABCA1 protein levels were detected by Western blot analysis. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control; n.s., not significant versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Suppressive effects of Ucn1 on foam cell formation & related protein expression in human monocyte-derived macrophages.Human monocytes were incubated for 7 days with RPMI-1640 supplemented with 10% human serum & the indicated concentrations of Ucn1, followed by a 19 h-incubation with 50 µg/ml oxLDL in the presence of 0.1 mmol/l [3H]oleate. Intracellular CE accumulation was determined from the radioactivity of cholesterol-[3H]oleate. Otherwise, before the addition of oxLDL, cells were harvested & subjected to immunoblotting analyses for CD36, ACAT1, or ABCA1. beta -Actin served as a loading control. Data are expressed as means ± SEM from 4–6 independent experiments with monocytes from 4–6 different donors. Baseline (1 fold) = 8.4±2.2 nmol/mg cell protein. *P<0.05, †P<0.001 vs. 0 nmol/l of Ucn1. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Effect of evodiamine on ABCA1 transcription & the degradation rate of ABCA1 protein. (a) Differentiated THP-1 macrophages were incubated with 10 μM evodiamine or 10 μM pioglitazone as positive control for 24 h. Total RNA was extracted & ABCA1 mRNA expression levels were quantified by qRT-PCR. (b) 293 T cells were transfected with a luciferase reporter construct driven by the human ABCA1 promoter as described in the Materials & Methods section. After transfection, cells were treated with 10 μM Evodiamine or 1 μM T0901317 as positive control for 24 h. (c) Differentiated THP-1 macrophages were incubated for 24 h with (black circle) or without (Veh; white circle) evodiamine (10 μM) & lysed after addition of cycloheximide (CHX; 140 μM) at different time points (0, 10, 20, 40 min). Western blot analysis shows the decline of ABCA1 protein level with cycloheximide in the presence & absence of evodiamine. All data are means ± S.D. (n = 3) vs. solvent vehicle control (DMSO), *p < 0.05, **p < 0.01, n.s. no significance (ANOVA/Bonferroni). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30038271), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - miR-33 deficiency improved cholesterol efflux in macrophages. A, Quantitative real-time PCR analysis of Abca1 & Abcg1 in macrophages from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. Values from miR-33+/+Apoe−/− were set at 100%. Values are mean±SE (n=7 each); ***P<0.001. B, Western blotting analysis of ABCA1 & ABCG1 in thioglycollate-elicited peritoneal macrophages from miR-33+/+Apoe−/− & miR-33−/−Apoe−/− mice. GAPDH was used as a loading control. C, Cholesterol efflux from thioglycollate-elicited peritoneal macrophages in the presence or absence of apoA-I (5 or 10 μg/mL). Values are mean±SE (n=6 each); **P<0.01. D, Cholesterol efflux from thioglycollate-elicited peritoneal macrophages in the presence or absence of HDL-C (50 or 100 μg/mL). Values are mean±SE (n=6 each); **P<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23316322), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - A–C) Effects of 6‐dihydroparadol & specific proteasomal, lysosomal, & calpain inhibitors on the ABCA1 protein levels. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with or without 6‐dihydroparadol (6‐DP, 30 μm) for 24 h & incubated for another 3 h with or without the proteasome inhibitor lactacystin (10 μm), the lysosomal inhibitor chloroquine (100 μm), or the calpain inhibitor calpeptin (30 μg mL−1). The ABCA1 protein levels were detected by Western blot analysis. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control; n.s., not significant versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Srebf1 is a miR-33 target gene.(a) Relative changes in lipid metabolism-related genes in the livers of miR-33−/− mice compared with miR-33+/+ mice fed NC at 16 weeks of age. (n=5–8 each,*P<0.05 in Student’s t-test). (b) Conservation of miR-33 target regions in the 3′UTR of Srebf1. Underlined sequences are the potential binding site of miR-33 seed sequences. * indicates the conservation among spieces. (c) 3′UTR reporter assay used to verify the target. Luciferase reporter activity of human & mouse SREBP-1 gene 3′UTR constructs in 293T cells overexpressing miR-control (miR-Con) & miR-33 (n=4 each, *P<0.05 & ***P<0.001 in Student’s t-test). (d) miR-33 dose-dependent changes in luciferase reporter activity of mouse Srebf1 3′UTR construct in 293T cells. miR-Con & miR-146a is used as a negative control (n=4 each, *P<0.05 & ***P<0.001 in one-way analysis of valiance test). (e) Luciferase reporter activity of the WT or mutant Srebf1 3′UTR at the potential miR-33 binding site in 293T cells (n=4 each, **P<0.01 in Student’s t-test). (f,g) Luciferase reporter activity of SRE-promoter (f) or FAS-promoter (g) in 293T cells. 293T cells were co-transfected with mouse Srebf1 with the full-length 3′UTR or without the 3′UTR, along with expression plasmids for miR-negative control, or miR-33. Values are the mean±s.e. (n=4 each, **P<0.01 versus miR-Con. ***P<0.001 versus miR-Con in one-way analysis of valiance test). (h) Western blotting analysis of SREBP-1, ABCA1, & IRS-2 in miR-33 transduced HepG2 cells & primary hepatocytes & hepatocytes prepared from miR-33+/+ & miR-33−/− mice. Representative western blot images are shown (n=4). Values are the means±s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24300912), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Chemical structure, (B) cytotoxicity of alpha -asarone, (C) enhancement of cholesterol efflux by alpha -asarone, (D & E) upregulation of ABCA1 & ABCG1 by alpha -asarone & beta -asarone, & (F) elevation of retinoid X receptor (RXR) alpha transcription. J774A.1 murine macrophages were exposed to 50 μg/ml oxidized low-density lipoprotein (LDL) & treated with 1–10 μM purple Perilla frutescens extracts (PPE)-alpha -asarone & 5–10 μM beta -asarone. (B) MTT assay was performed for the measurement of alpha -asarone toxicity. Graph data represent 1 of 4 independent experiments with multiple estimations. Values are expressed as the percentage cell survival relative to the untreated control cells (cell viability, 100%). (C) Cholesterol efflux was expressed as the percentage fluorescence in the medium relative to total fluorescence. (D & E) For the measurement of ABCA1 & ABCG1 expression, total cell lysates were subjected to western blot analysis with a primary antibody against ABCG1 or ABCG1. beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. Bar graphs denoted without a common letter indicate significant difference, P<0.05. (F) RXR alpha mRNA expression was measured by RT-PCR. GAPDH was used as a housekeeping gene for the co-amplification with RXR alpha . Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Bilirubin significantly decreases expression of ATP‐binding cassette transporter A1 (ABCA1) protein in THP‐1 macrophages treated with LXR agonist (TO901317) to upregulate ABCA1 protein. THP‐1 cells were differentiated for 72 hours with 200 nmol/L phorbol‐12‐myristate‐13‐acetate & then loaded with unlabeled cholesterol for another 24 hours. Cells were treated with TO901317 (5 μmol/L) or solvent vehicle (0.1% dimethyl sulfoxide [DMSO]) for 24 hours. Afterward, cells were treated with bilirubin (17.1 μmol/L) or solvent vehicle (0.1% DMSO) for 16 hours. The protein levels of ABCA1 were detected by western blot analysis. The bar graphs present mean±SD from 3 independent experiments. ***P<0.001 vs control (determined by ANOVA with Bonferroni post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28455345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Induction of cholesterol efflux attenuates the enhancement in HIV-1infectivity in NPCD55 cells. (A) Normal & NPCD55 cells were treated with 5 μM TO-901317 at 24 h post-infection & cultured for 72 h in the presence of the compound. At 96 h post-infection the cells were harvested & ABCA1, Gag, & beta -actin expression was detected by Western blotting analysis. All samples were loaded on the same gel. (B) AmplexRed assay was performed to measure free cholesterol content of untreated (filled) & TO-901317-treated (slashed) cells. Cholesterol content was normalized to protein concentration. (C) AmplexRed assay was performed to measure virion-associated cholesterol from purified virus produced in untreated (filled) & TO-901317-treated (slashed) infected cells. Virion-associated cholesterol content was normalized to p24 concentration. (D) TZM-bl reporter assay was performed to measure virus infectivity when cholesterol efflux was induced via TO-901317 stimulation. The ρ values were calculated by performing student T-test (*denotes < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22273177), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Effects of apabetalone on ABCA1 expression & carboplatin response in ovarian cancer cells. (A) Apabetalone treatment reduced expression of ABCA1 in OVCAR-5 & OVCAR-5 CBPR cell lines. Cells were treated for 72 h with control medium (DMSO) or apabetalone (80 µM). Protein extracts from OVCAR-5 (~30 µg) were electrophoresed & immunoblotted with rabbit polyclonal ABCA1 antibody (1/1000, NB400-105, Novus Biological) & beta -actin (1/2000, Abcam) was used as a loading control. (B) Quantification of Western blot. OVCAR-5 (C), CaOV3 (D), OVCAR-5 CBPR (E) & CaOV3 CBPR (F) cell survival following treatment with carboplatin (CBP) alone (0-200 µM, black line) & in combination with apabetalone (Apa, 80 µM, purple line). (G) Carboplatin IC50 for OVCAR-5 & OVCAR-5 CBPR cells ± apabetalone. Data are mean ± SEM from three independent experiments. *P < 0.05, Student’s t-test. (H) Carboplatin IC50 for CaOV3 & CaOV3 CBPR cells ± apabetalone. Data are mean ± SEM from three independent experiments. P < 0.05, Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35582032), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Expression of ATP‐binding cassette transporter A1 (ABCA1) protein in THP‐1 macrophages treated with bilirubin & in peripheral blood mononuclear cells (PBMCs) from Gilbert syndrome (GS) patients. A, Bilirubin suppresses the expression of ABCA1 protein in THP‐1‐derived macrophages time‐dependently. THP‐1 cells were differentiated as described in Figure 1 & then loaded with unlabeled cholesterol for another 24 hours. Cells were treated with bilirubin (17.1 μmol/L) for 4, 8, 16, & 24 hours. The protein levels of ABCA1 were detected by western blotting. Control was treated with solvent vehicle (0.1% dimethyl sulfoxide). The bar graph presents mean±SD from 3 independent experiments. B, Expression of ABCA1 protein is decreased in PBMCs from participants with high bilirubin blood levels (GS) compared with healthy controls. The protein levels of ABCA1 were detected by western blotting. The bar graph presents mean±SEM (n=28 per group). *P<0.05, **P<0.01 & ***P<0.001 vs control. ns indicates not significant (determined by Student t test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28455345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Time course response of ABCA1 induction by pioglitazone, & (B) enhancement of ABCA1 & (C) peroxisome proliferator-activated receptor gamma (PPAR gamma ) by purple Perilla frutescens extracts (PPE). J774A.1 murine macrophages were incubated with 10 μM pioglitazone & 50 μg/ml oxidized low-density lipoprotein (LDL) in the absence or presence of 1–10 μg/ml PPE. For the measurement of expression of (A & B) ABCA1 & (C) PPAR gamma , total cell lysates were subjected to western blot analysis with a primary antibody against ABCA1 or PPAR gamma . beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. Bar graphs denoted without a common letter indicate significant difference, P<0.05. Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - LXR agonist-stimulated ABCA1 expression & function in patient-derived iRPEs. Patient-derived iRPE lines were stimulated for 16 h with LXR agonist & are relative to unstimulated cells shown in Figure 2. (A) Expression of ABCA1 mRNA normalized to RPL28 & relative to unstimulated cells shown in Figure 2B. (B) Western blot analysis of ABCA1 protein levels. Actin was detected as loading control. ABCA1 expression was quantified & normalized to actin. (C) Cholesterol efflux assay after direct cell labeling & in the presence of ApoAI. (D) Cholesterol efflux assay after phagocytosis of BODIPY-cholesterol-loaded POSs & in the presence of ApoAI. Data are relative to unstimulated cells shown in Figure 2F,G, respectively. Data are presented as means ± SD (n = 3). Unpaired Student’s t-test. * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328615), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition by LY294002 or Akt1/2 also enhances cholesterol efflux to apoA-I from ABCA1-expressing BHK cells.A) BHK cells were induced as in Fig. 1 & then incubated with LY294002 (200 µM) for 2 h. Cells were then lysed & analysed for ABCA1, phosphorylated Akt (p-Akt) & total Akt by Western-blotting. Hsp70 was also blotted as loading control. B & C) BHK cells were labeled with [3H] cholesterol & induced with 10 nM mifepristone overnight. Cholesterol efflux was measured after 2 h incubation with BSA (1 mg/ml) or BSA plus apoA-I (5 µg/ml). Some of cells were also incubated with indicated doses of LY294002 (B) or Akt1/2 (C), in addition to apoA-I, during 2 h efflux period. Results are presented as the average of triplicate wells with standard deviation & representative of more than three independent experiments. *** P<0.0001, **P<0.001 & *P<0.05 vs apoA-I only. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113789), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Generation, differentiation & analysis of ABCA1-deficient iRPEs. (A) Immunofluorescence staining for ZO-1 (red) in 4-week cultured iRPEs. Nuclei were stained with DAPI (blue). Scale bar = 100 μm. (B) Bright-field microscopy of iRPEs. (C) Ct values of RPE marker genes BEST1, OTX2, RLBP1 & RPE65 in iRPEs & parental iPSCs obtained by qPCR. Values shown are means ± SD (n = 3). Unpaired Student’s t-test. **** p < 0.0001. (D) Sequence alignment of parts of exon 14 (top) & exon 46 (bottom) of ABCA1-deficient iRPE cell clones & parental line. (E) Western blot of ABCA1 protein levels in ABCA1-deficient iRPE cell lines & parental cell line after 16 h of stimulation with 1 μM LXR agonist. Actin was detected as loading control. (F) Relative expression of ABCA1 mRNA in ABCA1-deficient iRPE cell lines & parental cell line normalized to ACTB. Values shown are means ± SD (n = 3). One-way ANOVA with Tukey’s post hoc test. **** p < 0.0001. (G) Relative expression of ABCG1 mRNA in ABCA1-deficient iRPE cell lines & parental cell line normalized to ACTB. Values shown are means ± SD (n = 3). One-way ANOVA with Tukey’s post hoc test. ** p < 0.01; *** p < 0.001. (H) Cholesterol efflux assay in iRPEs in the presence of ApoAI and/or LXR agonist (LXR ag). Values shown are means ± SD (n = 3). Two-way ANOVA with Bonferroni post hoc test vs. DMSO + BSA control. **** p < 0.0001. (I) Bright-field (BF) microscopy & Nile Red fluorescence microscopy (overview & close-up view marked with white squares) of 4-week cultured ABCA1-deficient iRPE cell lines & parental cell line. Relative fluorescence was quantified & is shown as mean ± SD (n = 8). One-way ANOVA with Tukey’s post hoc test. * p < 0.05, **** p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328615), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition enhances cholesterol efflux to apoA-I from wt MEFs, but not from TSC2-/- MEFs where mTORC1 is constitutively activated.A) Mouse embryonic fibroblasts (MEFs), wt & TSC2-/-, were induced overnight with T0901317 (10 µM). Cell lysates were collected for Western blotting for phosphorylated S6K & total S6K. B) wt & TSC2-/- MEFs were incubated with or without T0901317 (10 µM) overnight. Some of cells were then incubated with apoA-I (10 µg/ml) or apoA-I plus DEBC (25 µM) for 2 h. The expression of ABCA1, phosphorylated Akt, total Akt & loading control hsp70 were analyzed by Western blotting. C) wt & TSC2-/- were labeled with [3H] cholesterol for 1 day & then incubated with or without T0901317 (10 µM) overnight. Some of cells were then incubated with apoA-I (10 µg/ml) or apoA-I plus DEBC (25 µM) for 2 h to analyze cholesterol efflux. Data presented as the average of triplicate wells with standard deviation & representative of at least three independent experiments. *** P<0.0001 vs apoA-I only. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113789), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Akt inhibition by DEBC enhances cholesterol efflux to apoA-I specifically from ABCA1-expressing BHK cells.A) BHK-ABCA1 cells were induced with mifepristone (10 nM) overnight & then incubated with indicated doses of DEBC for 2 h. Cells were then lysed & Western-blotted for ABCA1, phosphorylated Akt (p-Akt) & total Akt. Hsp70 was also blotted as loading control. B) BHK-ABCA1 cells were labeled with [3H] cholesterol & induced overnight as above. After 2 h incubation with BSA (1 mg/ml) or BSA plus apoA-I (5 µg/ml), cholesterol efflux was measured as described in the Methods section. Some of the cells were also incubated with indicated doses of DEBC, in addition to apoA-I, during 2 h efflux period. C) BHK-ABCA1 & BHK-A937V cells were induced with mifepristone (10 nM) overnight. 2 h Cholesterol efflux was measured as above either with or without DEBC (25 µM). Results are presented as the average of triplicate wells with standard deviation & representative of more than three independent experiments. *** P<0.0001 & **P<0.001 vs apoA-I only. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113789), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - LXRs control expression of genes involved in cholesterol homeostasis & fatty acid synthesis in MPECs.(A) qPCR analysis of Abca1, Abcg1 & Idol levels in WT (+/+) & Lxr alpha beta −/− (lxr−/−) MPECs after DMSO (vehicle) or T0901317 stimulation (B) Effect of 9-cis retinoic acid and/or T0901317 stimulation on Abca1 accumulation levels in WT (+/+) & Lxr alpha beta −/− (lxr−/−) MPECs (C) qPCR analysis of Srebp1, Acc, & Fas levels in WT (+/+) & Lxr alpha beta −/− (lxr−/−) MPECs after DMSO (vehicle) or T0901317 stimulation. (qPCR analysis results from 4 independant experiments & was normalized using 36b4 gene). *p<0.05, **p<0.01, ***p<0.001 in Student’s t test. Error bars represent mean ± SEM. (D) Western blot analysis was performed on WT (+/+) or Lxr alpha beta −/− MPECs using Srebp1c, Abca1 & beta -Actin antibodies. (E) Oil-Red O staining (ORO) & Normarski/Dapi of WT (+/+) & Lxr alpha beta −/− MPECs, or WT MEFs, treated for 48h with DMSO (vehicle) or T0901317 (1 µM). Head arrows indicate lipid droplets. Scale bars 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23554947), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Time course response of ABCA1 induction by pioglitazone, & (B) enhancement of ABCA1 & (C) peroxisome proliferator-activated receptor gamma (PPAR gamma ) by purple Perilla frutescens extracts (PPE). J774A.1 murine macrophages were incubated with 10 μM pioglitazone & 50 μg/ml oxidized low-density lipoprotein (LDL) in the absence or presence of 1–10 μg/ml PPE. For the measurement of expression of (A & B) ABCA1 & (C) PPAR gamma , total cell lysates were subjected to western blot analysis with a primary antibody against ABCA1 or PPAR gamma . beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. Bar graphs denoted without a common letter indicate significant difference, P<0.05. Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Anti-ApoA-1 IgGs upregulate ABCA1. ABCA1 was increased after 16 & 24 h of anti-ApoA-1 IgG exposure to HMDM at the mRNA level as revealed by RT-PCR (a) as well as at the protein level after 24 h anti-apoA-1 IgG stimulation, as revealed by Western blot analysis (b). In panel a, data are expressed as fold change expression of the mean ± SD of ABCA1 calculated by delta delta CT method of six independent experiments (n = 6) & values were normalized to untreated condition. p-values were calculated using the Student’s t-test: * p < 0.05, ** p < 0.01, **** p < 0.0001. (b) One of four representative Western blots is shown. Data are the mean ± SD of band intensity volume/actin intensity volume of four independent experiments (n = 4). p-values were calculated using the Student’s t-test: * p = 0.02, ** p = 0.005. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31766415), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - A–C) Effects of 6‐dihydroparadol & specific proteasomal, lysosomal, & calpain inhibitors on the ABCA1 protein levels. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with or without 6‐dihydroparadol (6‐DP, 30 μm) for 24 h & incubated for another 3 h with or without the proteasome inhibitor lactacystin (10 μm), the lysosomal inhibitor chloroquine (100 μm), or the calpain inhibitor calpeptin (30 μg mL−1). The ABCA1 protein levels were detected by Western blot analysis. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control; n.s., not significant versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - ABCA1 protein expression is upregulated in ovarian cancer cells with acquired carboplatin resistance. ABCA1 expression in OVCAR-5 (A) & CaOV3 (C) & carboplatin resistant OVCAR-5 CBPR (B) & CaOV3 CBPR (D) by immunocytochemistry using rabbit polyclonal ABCA1 antibody (1/100, NB400-105, Novus Biological). (E) OVCAR-5 cells with Rabbit IgG & (F) CaOV3 cells with Rabbit IgG. (G) Protein extracts from OVCAR-5 (~30 µg) & CaOV3 cell lines (~60 µg) were electrophoresed & immunoblotted with rabbit polyclonal ABCA1 antibody (1/1000, NB400-105, Novus Biological), & beta -actin (1/2000, Abcam) was used as a loading control. A major band was detected at ~250 kDa, which is the predicted size for ABCA1. (H) Quantitation of ABCA1 Western blots. Data are from 2-4 independent experiments. Statistical significance was determined using the Student’s t-test, *P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35582032), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Evodiamine enhances apo A1-mediated ChE from THP-1 macrophages & increases ABCA1 protein level. (a) Differentiated THP-1 cells were loaded with [3H]-cholesterol together with the indicated treatments for 24 h. On the next day, the cells were washed twice with PBS & incubated with the same compounds [solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (1–20 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control] with or without 10 µg/mL apo A1. Extracellular as well as intracellular radioactivities were quantified with scintillation counter. Differentiated THP-1-derived macrophages were treated with solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (10 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control. After 24 h incubation, the cells were lysed & 20 μg protein was resolved via SDS-PAGE. Immunodetection was performed with antibodies against the indicated proteins, ABCA1 (b), ABCG1 (c), & SR-B1 (d), & visualized by chemiluminescence detection. All experiments were performed at least three times & data are presented as means ± S.D. vs. solvent vehicle control, *p < 0.05, **p < 0.01, ***p < 0.001, n.s. no significance (ANOVA/Bonferroni). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30038271), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - A–C) Effects of 6‐dihydroparadol & specific proteasomal, lysosomal, & calpain inhibitors on the ABCA1 protein levels. THP‐1 cells were differentiated as described in Figure 2, & then loaded with unlabeled cholesterol for 24 h. Cells were treated with or without 6‐dihydroparadol (6‐DP, 30 μm) for 24 h & incubated for another 3 h with or without the proteasome inhibitor lactacystin (10 μm), the lysosomal inhibitor chloroquine (100 μm), or the calpain inhibitor calpeptin (30 μg mL−1). The ABCA1 protein levels were detected by Western blot analysis. The bar graphs represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, & ***p < 0.001 versus control; n.s., not significant versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Functional miR-33 responsive elements in the 3′UTR of ATP8B1 & ABCB11A,B. Conserved sequences in the 3′UTR of ATP8B1 & ABCB11 are partially complementary to miR-33. The element in human ATP8B1 is located 1877–1897 nt after the stop codon. The element in ABCB11 overlaps the stop codon in primates, while rodents show a conserved sequence 732–751 nt after the stop codon.C,D. Luciferase assays in HEK293 cells using the whole 3′UTR of human or murine ATP8B1 & ABCB11, or a single copy of the responsive elements (RE) identified above, or mutant responsive elements (RE*; where AATGCA was mutated to GGGTTG to prevent complementarity to the seed sequence of the miRNA), co-transfected with (closed bars) or without (open bars) a vector to overexpress miR-33. In grey, data from empty (negative control) & R33 (positive control containing a 100% match to miR-33) reporter vectors.E,F. Relative mRNA expression of canalicular transporters in primary murine hepatocytes (n = 4 dishes/condition) & human HuH-7 hepatoma cells (n = 3 dishes/condition) transduced 48 h with empty or miR-33 adenovirus.G. Relative protein levels in HuH7 cells transduced with empty or miR-33 adenovirus. Some cells were incubated for 16 h in the presence of FXR:RXR agonists (2 µmol/L GW4064 : 1 µmol/L 9-cis-retinoic acid) to induce ABCB11. Asterisk indicates a non-specific band. Data shown as mean ± SD; **p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22767443), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Chemical structure, (B) cytotoxicity of alpha -asarone, (C) enhancement of cholesterol efflux by alpha -asarone, (D & E) upregulation of ABCA1 & ABCG1 by alpha -asarone & beta -asarone, & (F) elevation of retinoid X receptor (RXR) alpha transcription. J774A.1 murine macrophages were exposed to 50 μg/ml oxidized low-density lipoprotein (LDL) & treated with 1–10 μM purple Perilla frutescens extracts (PPE)-alpha -asarone & 5–10 μM beta -asarone. (B) MTT assay was performed for the measurement of alpha -asarone toxicity. Graph data represent 1 of 4 independent experiments with multiple estimations. Values are expressed as the percentage cell survival relative to the untreated control cells (cell viability, 100%). (C) Cholesterol efflux was expressed as the percentage fluorescence in the medium relative to total fluorescence. (D & E) For the measurement of ABCA1 & ABCG1 expression, total cell lysates were subjected to western blot analysis with a primary antibody against ABCG1 or ABCG1. beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. Bar graphs denoted without a common letter indicate significant difference, P<0.05. (F) RXR alpha mRNA expression was measured by RT-PCR. GAPDH was used as a housekeeping gene for the co-amplification with RXR alpha . Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - Gas6 treatment enhances expression of LXR alpha & LXR beta & their target genes in BMDM.Mouse BMDM were stimulated with 400 ng/ml Gas6, 1 μM T0901317, 10 ng/ml interferon (IFN)-gamma , 10 ng/ml IL-4, or 100 ng/ml LPS for 4 h (a,b) or 400 ng/ml Gas6 for the indicated times (c–g). (a,b,e) The amounts of the LXR alpha , LXR beta , ABCA1, ABCG1, ApoE, AIM, Arg2, VEGF, YM1, & Arg1 mRNAs were analyzed by real-time PCR & normalized to that of Hprt mRNA. (c,d,f,g) The relative abundances of LXR alpha , LXR beta , ABCA1, ABCG1, ApoE, Aim, Arg2, & VEGF proteins were determined by Western blotting analysis. The relative densitometric intensity was determined for each band & normalized to beta -actin. Data in all bar graphs are means ± SEM of three independent experiments. *P < 0.05 compared with control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27406916), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ABCA1 Antibody - BSA Free [NB400-105] - (A) Time course response of ABCA1 induction by TO-091317 & (B) upregulation of ABCA1 by purple Perilla frutescens extracts (PPE), & (C) enhancement of liver X receptor (LXR) alpha induction by PPE. J774A.1 murine macrophages were cultured with 1 μM TO-091317 or 50 μg/ml Cu2+-oxidized low-density lipoprotein (LDL) in the absence or presence of 1–10 μg/ml PPE. For the measurement of expression of (A & B) ABCA1 & (C) LXR alpha , total cell lysates were subjected to western blot analysis with a primary antibody against ABCA1 or LXR alpha . beta -actin was used as an internal control. Bar graphs (means ± SEM, n=3) represent quantitative densitometric results of the upper bands. Bar graphs denoted without a common letter indicate significant difference, P<0.05. Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijmm.2015.2101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, Rb
• Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IP, WB, B/N, ChIP, Dual ISH-IHC, Func, GS, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ABCA1 Antibody - BSA Free Summary

• Immunogen: Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.
• Localization: Astrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize reported in scientific literature (PMID 30821416)
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 19515742)
  • Dual RNAscope ISH-IHC
  • ELISA reported in scientific literature (PMID 18541924)
  • Flow Cytometry 1:400. Use reported in scientific literature (PMID 21501868)
  • Gel Super Shift Assays reported in scientific literature (PMID 15684432)
  • Gel Supershift Assay
  • Immunoblotting reported in scientific literature (PMID 27599291)
  • Immunocytochemistry/ Immunofluorescence 1:100. Use reported in scientific literature (PMID 21501868)
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 21846716; 21106520)
  • Knockdown Validated reported in scientific literature (PMID 31666189)
  • Knockout Validated reported in scientific literature (PMID 32273567)
  • PCR reported in scientific literature (PMID 27406916)
  • Simple Western 1:50
  • Western Blot 1:500
• Application Notes: Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: separated by Charge, antibody dilution of 1:50Separated by Charge.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raw 264.7 Whole Cell - T0901317 treated Assay Kit
  THP-1 TO901317 Treated / Untreated Cell Lysate

• Control Peptide:

  ABCA1 Protein (NB400-105PEP)

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA1 Antibody - BSA Free

• ABC1
• ABC-1
• ABC1ATP-binding cassette transporter A1
• ABCA1
• ATP-binding cassette 1
• ATP-binding cassette transporter 1
• ATP-binding cassette, sub-family A (ABC1), member 1
• CERP
• Cholesterol efflux regulatory protein
• EC 2.7.7.8
• EC 2.8.1.8
• EC 3.6.3
• EC 3.6.3.41
• FLJ14958
• HDLDT1
• membrane-bound
• MGC164864
• MGC165011
• TGD
• TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA1 Antibody (NB400-105)(414)

Publications using NB400-105

• Xue C, Zeng P, Gong K et Al. Nogo-B inhibition facilitates cholesterol metabolism to reduce hypercholesterolemia Cell Rep 2024-09-03 [PMID: 39235944]
• Xia J, Wang W, Xia X et al. Triptolide suppresses secretion of inflammatory factors and promotes expression of ABCA1 International Journal of Clinical and Experimental Medicine 2019-06-30 (WB, Mouse)
• Dubland JA. Low lysosomal acid lipase activity in smooth muscle cells relative to macrophages provides new insights into foam cell formation in atherosclerosis. Thesis. 2018-01-01 (WB, Human)
• Huynh TN, Fikse EN, De La Torre AL et Al. Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Aging Apolipoprotein E4 Mice Alters Their Brains' Inflammatory Profiles Int J Mol Sci 2024-12-21 [PMID: 39769453]
• Eva Tsaousidou, Jędrzej Chrzanowski, Pascal Drané, Grace Y Lee, Nadine Bahour, Zeqiu Branden Wang, Shijun Deng, Zhe Cao, Kaimeng Huang, Yizhou He, Mateusz Kaminski, Dominika Michalek, Ekin Güney, Kalindi Parmar, Wojciech Fendler, Dipanjan Chowdhury, Gökhan S Hotamışlıgil Endogenous p53 inhibitor TIRR dissociates systemic metabolic health from oncogenic activity. Cell reports 2024-07-01 [PMID: 38861384]
• Ma C, Zhang J, Yang S et al. Astragalus flavone ameliorates atherosclerosis and hepatic steatosis via improving lipid metabolism and inhibition of inflammation in apoE-/- mice Research Square Sep 3 2020
• Fabiano M, Oikawa N, Kerksiek A, Furukawa JI et Al. Presenilin Deficiency Results in Cellular Cholesterol Accumulation by Impairment of Protein Glycosylation and NPC1 Function Int J Mol Sci 2024-05-25 [PMID: 38791456]
• Juan Vladimir de la Rosa, Carlos Tabraue, Zhiqiang Huang, Marta C. Orizaola, Patricia Martin‐Rodríguez, Knut R. Steffensen, Juan Manuel Zapata, Lisardo Boscá, Peter Tontonoz, Susana Alemany, Eckardt Treuter, Antonio Castrillo Reprogramming of the LXR alpha Transcriptome Sustains Macrophage Secondary Inflammatory Responses Advanced Science 2024-03-28 [PMID: 38549193]
• Stefanie Steinbauer, Alice König, Cathrina Neuhauser, Bettina Schwarzinger, Herbert Stangl, Marcus Iken, Julian Weghuber, Clemens Röhrl Elder ( Sambucus nigra ), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis Scientific Reports 2024-02-12 [PMID: 38347122]
• Ren Sheng, Yining Li, Yexiang Wu, Chang Liu, Weizhi Wang, Xiaowan Han, Yinghong Li, Lijuan Lei, Xinhai Jiang, Yuyan Zhang, Yuhao Zhang, Shunwang Li, Bin Hong, Chao Liu, Yanni Xu, Shuyi Si A pan-PPAR agonist E17241 ameliorates hyperglycemia and diabetic dyslipidemia in KKAy mice via up-regulating ABCA1 in islet, liver, and white adipose tissue. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024-03-04 [PMID: 38308968]
• Carneiro de Oliveira K, Wei Y, Repetti RL, Meth J et Al. Tubular deficiency of ABCA1 augments cholesterol- and Na(+)-dependent effects on systemic blood pressure in male mice Am J Physiol Renal Physiol 2023-12-28 [PMID: 38153852]
• Mao M, Deng Y, Wang L et al. Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE(-/-) mice PeerJ 2023-09-04 [PMID: 37692113] (Western Blot, Human, Mouse)
• Wiener JP, Desire S, Garliyev V et al. Down-Regulation of ABCA7 in Human Microglia, Astrocyte and THP-1 Cell Lines by Cholesterol Depletion, IL-1? and TNF?, or PMA Cells 2023-08-25 [PMID: 37681876] (Western Blot, Human, Mouse)
• Resetar M, Tietcheu Galani BR, Tsamo AT et al. Flindissone, a Limonoid Isolated from Trichilia prieuriana, Is an LXR Agonist Journal of Natural Products 2023-08-25 [PMID: 37526502] (Western Blot, Human, Mouse)
• Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (Western Blot, Human, Mouse)
• Molina-Gonzalez I, Holloway RK, Jiwaji Z et al. Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration Nature communications 2023-06-08 [PMID: 37291151] (ICC/IF, Mouse)
• Miyagawa S, Horie T, Nishino T et al. Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitis Life science alliance 2023-08-01 [PMID: 37263777] (WB, Mouse, Human)
• Cao D, Khan Z, Li X et al. Macrophage angiotensin-converting enzyme (ACE) reduces atherosclerosis by increasing PPAR? and fundamentally changing lipid metabolism Cardiovascular research 2023-05-24 [PMID: 37225143] (WB, Mouse)
• Dragoljevic D, Lee MKS, Pernes G et al. Administration of an LXR agonist promotes atherosclerotic lesion remodelling in murine inflammatory arthritis Clinical & translational immunology 2023-04-18 [PMID: 37091327] (IHC, Mouse)
  
  Details:
  Mouse lesions in arthritic mice
• Cashikar A, Rios D, Timm D et al. Regulation of astrocyte lipid metabolism and ApoE secretion by the microglial oxysterol, 25-hydroxycholesterol Journal of lipid research 2023-02-25 [PMID: 36849076] (Mouse)
• Li Z, He M, Chen G et al. Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice bioRxiv : the preprint server for biology 2023-02-07 [PMID: 36798262] (WB, Mouse)
• Yalcinkaya M, Fotakis P, Liu W et al. Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1b secretion Cardiovascular research 2022-12-20 [PMID: 36537208] (WB, Mouse)
• Wang H, Zheng A, Arias EB, Cartee GD. Prior AICAR induces elevated glucose uptake concomitant with greater ?3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats Facets (Ott) 2022-11-16 [PMID: 36381195] (Western Blot, Human, Mouse)
• Papotti B, Adorni MP, Marchi C et al. PCSK9 Affects Astrocyte Cholesterol Metabolism and Reduces Neuron Cholesterol Supplying In Vitro: Potential Implications in Alzheimer's Disease International journal of molecular sciences 2022-10-13 [PMID: 36293049] (WB, Human)
• Cassim Bawa FN, Gopoju R, Xu Y et al. Retinoic Acid Receptor Alpha (RARa) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice Cells 2022-10-11 [PMID: 36291054] (WB, Mouse)
• Cai Y, Wang Z, Li L et al. Neuropeptide Y regulates cholesterol uptake and efflux in macrophages and promotes foam cell formation Journal of cellular and molecular medicine 2022-09-29 [PMID: 36172879] (WB, Human)
• Man B, Hu C, Yang G et al. Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPAR alpha in ApoE-/- mice Biochemical and Biophysical Research Communications 2022-07-01 [PMID: 35933927] (WB, Mouse)
  
  Details:
  Figure 3E
• Wang JQ, Li LL, Hu A Et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Nature 2022-08-03 [PMID: 35922515] (WB)
  
  Details:
  Citation using the FITC version of this antibody.
• Perelroizen R, Philosof B, Budick-Harmelin N et al. Astrocyte immunometabolic regulation of the tumour microenvironment drives glioblastoma pathogenicity Brain : a journal of neurology 2022-07-28 [PMID: 35899587] (IHC-Fr, WB, Mouse)
• Vaisar T, Wang S, Omer M et al. 10,12-conjugated linoleic acid supplementation improves HDL composition and function in mice Journal of lipid research 2022-06-14 [PMID: 35714730] (WB, Mouse)
• My-Anh Nguyen, Huy-Dung Hoang, Adil Rasheed, Anne-Claire Duchez, Hailey Wyatt, Mary Lynn Cottee, Tyson E. Graber, Leah Susser, Sabrina Robichaud, İbrahim Berber, Michele Geoffrion, Mireille Ouimet, Hilal Kazan, Lars Maegdefessel, Erin E. Mulvihill, Tommy Alain, Katey J. Rayner miR-223 Exerts Translational Control of Proatherogenic Genes in Macrophages Circulation Research 2022-05-25 [PMID: 35611698]
• Wang W, Lokman N, Noye T et al. ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome Cancer Drug Resist 2022-05-18 [PMID: 35582032]
• Li J, Zhu Z, Li Y et al. D-4F, an apolipoprotein A-I mimetic, promotes the clearance of myelin debris and the reduction of foamy macrophages after spinal cord injury Bioengineered 2022-05-12 [PMID: 35546071] (Western Blot, Human, Mouse)
• Picataggi A, Rodrigues A, Cromley DA et al. Specificity of ABCA7-mediated cell lipid efflux Biochimica et biophysica acta. Molecular and cell biology of lipids 2022-07-01 [PMID: 35381375] (WB, Human)
• Yano H, Fujiwara Y, Horlad H et al. Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy Cancer science 2022-06-01 [PMID: 35343027] (WB, Human)
• Peters F, Ebner LJA, Atac D et al. Regulation of ABCA1 by AMD-Associated Genetic Variants and Hypoxia in iPSC-RPE International journal of molecular sciences 2022-03-16 [PMID: 35328615] (WB, Human)
• Yildirim Z, Baboo S, Hamid SM et al. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression EMBO molecular medicine 2022-02-22 [PMID: 35191199] (WB, Mouse)
• Balamurugan K, Medishetti R, Kotha J et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways iScience 2022-02-01 [PMID: 35141506] (WB, Human, Mouse)
• Toma L, Barbalata T, Sanda G Et al. CRISPR/dCas9 Transcriptional Activation of Endogenous Apolipoprotein AI and Paraoxonase 1 in Enterocytes Alleviates Endothelial Cell Dysfunction Biomolecules 2021-11-25 [PMID: 34944413] (WB, Human)
• Kane J, Jansen M, Hendrix S et al. Anti-Galectin-2 antibody treatment reduces atherosclerotic plaque size and alters macrophage polarity Thrombosis and haemostasis 2021-12-01 [PMID: 34852377] (IF/IHC, Mouse)
• Ma C, Wu H, Yang G, Xiang J et Al. Calycosin ameliorates atherosclerosis by enhancing autophagy via regulating the interaction between KLF2 and MLKL in apolipoprotein E gene-deleted mice Br J Pharmacol 2021-10-29 [PMID: 34713437]
• Huuska N, Netti E, Tulamo R et al. Serum Amyloid A Is Present in Human Saccular Intracranial Aneurysm Walls and Associates With Aneurysm Rupture Journal of Neuropathology & Experimental Neurology 2021-10-01 [PMID: 34534311] (Western Blot, Human, Mouse)
• Roberts LM, Schwarz B, Speranza E et al. Pulmonary infection induces persistent, pathogen-specific lipidomic changes influencing trained immunity iScience 2021-09-24 [PMID: 34522865]
• Casado ME, Huerta L, Marcos-DIaz A Et al. Hormone-sensitive lipase deficiency affects the expression of SR-BI, LDLr, and ABCA1 receptors/transporters involved in cellular cholesterol uptake and efflux and disturbs fertility in mouse testis Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-08-27 [PMID: 34461308] (IF/IHC, Mouse)
• Han Y, Onufer E, Huang L et al. Enterically derived high-density lipoprotein restrains liver injury through the portal vein Science 2021-07-23 [PMID: 34437091]
• Huang R, Hu Z, Chen X Et al. The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021-08-10 [PMID: 34378353] (Western Blot, Human, Mouse)
• Wright MB, Varona Santos J, Kemmer C Et al. Compounds targeting OSBPL7 increase ABCA1-dependent cholesterol efflux preserving kidney function in two models of kidney disease Nature communications 2021-08-02 [PMID: 34341345] (IF/IHC, Mouse)
• Mota AC, Dominguez M, Weigert A et al. Lysosome-Dependent LXR and PPAR delta Activation Upon Efferocytosis in Human Macrophages Frontiers in immunology 2021-05-07 [PMID: 34025647] (WB, Human)
• Zhang S, Li Z, Zhang Y et al. Ketone Body 3 Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a Mediated Calcium Influx Advanced Science 2021-03-01 [PMID: 33977048] (IHC-P, WB, Mouse)
• Patel S THE ROLE OF MYELOID GSK3a/B IN ATHEROSCLEROSIS AND ATHEROSCLEROTIC REGRESSION Thesis 2022-01-01 (IHC-P, Mouse)
• Navia-Pelaez JM, Choi SH, Dos Santos Aggum Capettini L et al. Normalization of cholesterol metabolism in spinal microglia alleviates neuropathic pain The Journal of experimental medicine 2021-07-05 [PMID: 33970188]
• Wang H, Arias EB, Cartee GD Reduced Membrane Cholesterol Content in Skeletal Muscle Is Not Essential for Greater Insulin-stimulated Glucose Uptake after Acute Exercise by Rats Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2021-03-25 [PMID: 33765397]
• Brampton C, Pomozi V, Chen LH et al. ABCC6 deficiency promotes dyslipidemia and atherosclerosis Scientific reports 2021-02-16 [PMID: 33594095] (ICC/IF, Mouse)
• Horie T, Nakao T, Miyasaka Y et al. microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity Nature communications 2021-02-16 [PMID: 33594062] (WB, Mouse)
• Ma C, Feng K, Yang X, et al. Targeting macrophage liver X receptor by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis British journal of pharmacology 2021-01-28 [PMID: 33506494]
• Ren Q, Xie X, Tang Y et al. Methyl tertiary-butyl ether inhibits THP-1 macrophage cholesterol efflux in vitro and accelerates atherosclerosis in ApoE-deficient mice in vivo J Environ Sci (China) 2020-12-18 [PMID: 33334519]
• Alajbegovic A, Holmberg J, Daoud F, et al. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells Vascular pharmacology 2021-01-28 [PMID: 33516965] (WB, Human)
• Lv JJ, Wang H, Cui HY, et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis Frontiers in cell and developmental biology 2020-01-08 [PMID: 33490072] (ICC/IF, WB, Mouse)
• Palafox-Sanchez V, Ying Z, Royes LFF, Gomez-Pinilla F The interaction between brain and liver regulates lipid metabolism in the TBI pathology Biochimica et biophysica acta. Molecular basis of disease 2021-01-12 [PMID: 33444711] (WB, Rat)
• Xu (???) Y, Liu (??) C, Han (???) X et al. E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice Arteriosclerosis, Thrombosis, and Vascular Biology 2021-06-01 [PMID: 33441025] (Western Blot, Human, Mouse)
• Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology 2020-12-14 [PMID: 33381046] (WB, Mouse)
• Fu Y, Sun Y, Wang M et al. Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism Cell Metab 2020-12-01 [PMID: 33186558]
• Mukherjee V, Ramakrishna P, Bora S, Kotteazeth S Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation Steroids 2020-11-07 [PMID: 33171131] (WB, Human)
  
  Details:
  Western blot analysis performed on HepG2 cells.
• Dodge JC, Jensen EH, Yu J et al. Neutral lipid cacostasis contributes to disease pathogenesis in amyotrophic lateral sclerosis J Neurosci 2020-10-13 [PMID: 33051352]
• Munir MT, Ponce C, Santos JM et al. VD3 and LXR agonist (T0901317) combination demonstrated greater potency in inhibiting cholesterol accumulation and inducing apoptosis via ABCA1-CHOP-BCL-2 cascade in MCF-7 breast cancer cells Mol Biol Rep 2020-10-01 [PMID: 32990902]
• Zhao D, Li J, Xue C et al. TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells J. Biol. Chem. 2020-09-22 [PMID: 32963108] (Mouse)
• Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics 2020-08-13 [PMID: 32929345] (WB, Mouse)
• Soo-Ho Choi, Keun-Young Kim, Guy A. Perkins, Sébastien Phan, Genea Edwards, Yining Xia, Jungsu Kim, Dorota Skowronska-Krawczyk, Robert N. Weinreb, Mark H. Ellisman, Yury I. Miller, Won-Kyu Ju AIBP protects retinal ganglion cells against neuroinflammation and mitochondrial dysfunction in glaucomatous neurodegeneration Redox Biology 2020-08-27 [PMID: 32896719]
• Venkat P, Zacharek A, Landschoot-Ward J et al. Exosomes derived from bone marrow mesenchymal stem cells harvested from type two diabetes rats promotes neurorestorative effects after stroke in type two diabetes rats Experimental neurology 2020-09-02 [PMID: 32889008] (IHC-P, Rat)
• Xia X, Xu Q, Liu M et al. Deubiquitination of CD36 by UCHL1 promotes foam cell formation Cell Death Dis 2020-08-15 [PMID: 32801299] (IP)
• Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research 2020-07-01 [PMID: 32621955] (WB, Mouse)
• Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. 2020-06-30 [PMID: 32602556] (WB, Rat)
• Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. 2020-05-08 [PMID: 32437644] (WB, Human)
• Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020-05-21 [PMID: 32437392] (WB, Human)
• Pathak P, Helsley RN, Brown AL et al. Small Molecule Inhibition of Gut Microbial Choline Trimethylamine Lyase Activity Alters Host Cholesterol and Bile Acid Metabolism Am. J. Physiol. Heart Circ. Physiol. 2020-04-24 [PMID: 32330092] (WB, Mouse)
• Hiebl V, Schachner D, Ladurner A et al. Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations Biol Proced Online 2020-04-11 [PMID: 32308567]
• Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. 2020-03-27 [PMID: 32292343] (WB, Rat)
• He Q, Wang F, Honda T et al. Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation Sci Rep 2020-04-09 [PMID: 32273567] (KO, Mouse)
• B Fuenzalida, C Cantin, S Kallol, L Carvajal, V Pastén, S Contreras-, C Albrecht, J Gutierrez, A Leiva Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia Sci Rep, 2020-03-24;10(1):5264. 2020-03-24 [PMID: 32210256]
• Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. 2020-02-28 [PMID: 32173266] (WB, Mouse)
• Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. 2020-05-04 [PMID: 32097464] (FLOW, Mouse)
• Cipollari, E, Szapary, H J Et al. Correlates and Predictors of Cerebrospinal Fluid Cholesterol Efflux Capacity from Neural Cells, a Family of Biomarkers for Cholesterol Epidemiology in Alzheimer's Disease. J Alzheimers Dis 2020-02-18 [PMID: 32065798] (WB, Rat)
• Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy 2020-01-16 [PMID: 31944172]
• Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. 2019-12-18 [PMID: 31864712] (WB, Rat)
• Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. 2019-11-14 [PMID: 31771811] (WB, Mouse)
• Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. 2019-11-25 [PMID: 31769014]
• Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med 2019-11-21 [PMID: 31766415] (WB, Human)
• Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. 2019-11-19 [PMID: 31744820] (WB, Mouse, Human)
• Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019-10-27 [PMID: 31666189] (KD, WB, ICC/IF, Human, Mouse)
• Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules 2019-10-18 [PMID: 31635211] (WB, Mouse)
• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019-09-12 [PMID: 31604677]
• Hennessy EJ, van Solingen C, Scacalossi KR et al. The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Nat Metab 2019-01-01 [PMID: 31410392] (WB, Human)
• Zhou Y, Bazick H, Miles JR et al. A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic mice Exp. Neurol. 2019-08-03 [PMID: 31386828] (WB, IHC-Fr, Mouse)
• Paul A, Lydic TA, Hogan R, Goo YH Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells Int J Mol Sci 2019-08-02 [PMID: 31382484] (WB, Mouse)
• Mukhamedova N, Hoang A, Dragoljevic D et al. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog. 2019-07-01 [PMID: 31344124] (ICC/IF, Human, Mouse)
• Koyama S, Horie T, Nishino T et al. Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice J Am Heart Assoc 2019-07-02 [PMID: 31242815] (IHC-Fr, Mouse)
• Rege SD, Luiz R, Tsai B et al. Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame it on Fructose? Mol Nutr Food Res 2019-05-13 [PMID: 31087499] (WB, Rat)
• Zhou Y, Miles JR, Tavori H et al. PMP22 regulates cholesterol trafficking and ABCA1-mediated cholesterol efflux J. Neurosci. 2019-05-06 [PMID: 31061090] (WB, Mouse)
• Tiwari P, Blank A, Cui C, Schoenfelt KQ et al Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer. J Exp Med. 2019-06-03 [PMID: 31053611] (FLOW, Human)
  
  Details:
  Citation using the DyLight 650 version of this antibody.
• Liao S, Zhou Q, Zhang Y. Elastic aortic wrap reduced aortic stiffness by partially alleviating the impairment of cholesterol efflux capacity in pigs J Diabetes Metab Disord 2018-08-07 [PMID: 30918842] (WB, Porcine)
• Storti F, Klee K, Todorova V et al. Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration Elife 2019-03-13 [PMID: 30864945] (WB, ICC/IF, Mouse)
• Margaryan NV, Hazard-Jenkins H, Salkeni MA et al. The Stem Cell Phenotype of Aggressive Breast Cancer Cells Cancers (Basel) 2019-03-08 [PMID: 30857267] (IF/IHC, Human)
• Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) 2019-03-01 [PMID: 30821416] (B/N, Mouse)
• Nakazeki F, Tsuge I, Horie T et al. MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons Clin. Sci. 2019-02-28 [PMID: 30777884] (WB, Human)
• Tang S, Tabet F, Cochran BJ et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle Sci Rep 2019-02-04 [PMID: 30718702] (WB, Human)
• Vaidya M, Jentsch JA, Peters S et al. Regulation of ABCA1-mediated cholesterol efflux by sphingosine-1-phosphate signalling in macrophages. J. Lipid Res. 2019-01-17 [PMID: 30655318] (WB, Mouse)
• Ramon-Vazquez A, de la Rosa JV, Tabraue C et al. Common and Differential Transcriptional Actions of Nuclear Receptors LXRa and LXRb in macrophages. Mol. Cell. Biol. 2019-01-02 [PMID: 30602495] (WB, Mouse)
• Moon SH, Huang CH, Houlihan SL et al. p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression Cell 2018-12-14 [PMID: 30580964] (WB, Human)
• Torres-Adorno AM, Vitrac H, Qi Y et al. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux. Oncogene. 2018-11-20 [PMID: 30459358] (Human)
• Zinkhan EK, Yu B, McKnight R. Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet. Reprod Sci. 2018-11-19 [PMID: 30453824] (Rat)
• Han Q, Yeung SC, Ip MSM, Mak JCW. Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model. Biochem. Pharmacol. 2018-11-09 [PMID: 30428911] (WB, Rat)
• O'Reilly ME, Kajani S, Ralston JC et al. Nutritionally Derived Metabolic Cues Typical of the Obese Microenvironment Increase Cholesterol Efflux Capacity of Adipose Tissue Macrophages. Mol Nutr Food Res. 2018-11-09 [PMID: 30411491] (WB, Mouse)
• Jin P, Bian Y, Wang K et al. Homocysteine accelerates atherosclerosis via inhibiting LXRa-mediated ABCA1/ABCG1-dependent cholesterol efflux from macrophages Life Sciences 2018-12-01 [PMID: 30393020] (WB, Human)
• Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019-01-01 [PMID: 30389579] (Mouse)
• Zhong S, Li L, Zhang YL et al. Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. J. Clin. Invest. 2018-10-30 [PMID: 30375985] (WB, Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Liao S, Wu H, Chen R. Apolipoprotein A1 mimetic peptide ATI-5261 reverses arterial stiffness at late pregnancy and early postpartum in a COMT-/- mouse model of preeclampsia. Clin Hypertens 2018-09-15 [PMID: 30237900] (WB, Mouse)
• de Neergaard R, Nielsen JE, Jorgensen A et al. Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology 2018-09-04 [PMID: 30182437] (IF/IHC, Human)
• Wang X, Luo J, Li N et al. E3317 promotes cholesterol efflux in macrophage cells via enhancing ABCA1 expression. Biochem. Biophys. Res. Commun. 2018-09-26 [PMID: 30173890] (WB, Human)
• Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International 2018-07-12 [PMID: 30105261] (WB, Rabbit)
• Lyssenko NN, Haider N, Picataggi A et al. Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium J. Lipid Res. 2018-08-03 [PMID: 30076206] (IF/IHC, WB, Human)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. 2018-07-23 [PMID: 30038271] (WB, Human)
• Chernick D, Ortiz-Valle S, Jeong A et al. HDL Mimetic Peptide 4F Mitigates AB-Induced Inhibition of ApoE Secretion and Lipidation in Primary Astrocytes and Microglia. J. Neurochem. 2018-07-20 [PMID: 30028014] (WB, Mouse)
• Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018-06-15 [PMID: 29906247] (Mouse)
• Zinkhan EK, Yu B, Schlegel A. Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1 Front Physiol 2018-05-29 [PMID: 29896121] (WB, Rat)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018-05-26 [PMID: 29802792] (WB, Human)
• Feng T, Liu P, Wang X et al. SIRT1 activator E1231 protects from experimental atherosclerosis and lowers plasma cholesterol and triglycerides by enhancing ABCA1 expression Atherosclerosis 2018-04-30 [PMID: 29787963] (WB, Mouse)
• Ishigami M, OgaSawara F, Nagao K et al. Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation. Sci Rep 2018-04-18 [PMID: 29670126] (WB, Human)
• Xie Z, Wang X, Liu X et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc. 2018-03-03 [PMID: 29502100] (WB, Mouse)
• Ahn SB, Jun DW, Jang K et al. Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease Korean J. Intern. Med. 2019-07-01 [PMID: 29466845]
• Han Shuhong, Zhuang Haoyang, Shumyak Stepan et al. Liver X Receptor Agonist Therapy Prevents Diffuse Alveolar Hemorrhage in Murine Lupus by Repolarizing Macrophages. Front Immunol 2018 [PMID: 29456535] (Flow, Mouse)
• Casali BT, Reed-Geaghan EG, Landreth GE. Nuclear receptor agonist-driven modification of inflammation and amyloid pathology enhances and sustains cognitive improvements in a mouse model of Alzheimer's disease. J Neuroinflammation. 2018-02-15 [PMID: 29448961] (WB, Mouse)
• Sallam T, Jones M, Thomas BJ, Wu X. Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat. Med. 2018-02-12 [PMID: 29431742] (WB)
• Li Zhiqiang, Kabir Inamul, Tietelman Gladys et al. Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death & Disease 2018-02-07 [PMID: 29415989] (WB, Mouse)
• Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor‑kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017-10-31 [PMID: 29375683] (WB, Mouse)
• Milligan S, Martin GG, Landrock D et al. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice. Biochim Biophys Acta Mol Cell Biol Lipids 2018-03-01 [PMID: 29307784] (Mouse)
• Widenmaier SB, Snyder NA, Nguyen TB et al. NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis Cell. 2017-11-16 [PMID: 29149604] (WB)
• Doran AC, Ozcan L, Cai B et al. CAMKIIg suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J. Clin. Invest. 2017-11-01 [PMID: 28972541] (WB)
• Storti F, Raphael G, Griesser V et al. Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells. Exp Eye Res 2017-09-21 [PMID: 28943268] (ICC/IF, Human)
• Pelletier RM, Akpovi CD, Chen L et al. Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes Am J Physiol Endocrinol Metab. 2017-12-31 [PMID: 28851737] (WB, Mouse)
• Liang Y, Li X, Zhang Y et al. Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells Attenuate Cigarette Smoke-Induced Cardiac Remodeling and Dysfunction Front Pharmacol 2017-08-14 [PMID: 28804458] (WB, Rat)
• Morizawa YM, Hirayama Y, Ohno N et al. Reactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway Nat Commun 2017-06-22 [PMID: 28642575] (IHC-Fr, Mouse)
• Wang D, Tosevska A, Heib EH et al. Bilirubin Decreases Macrophage Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Protein Expression J Am Heart Assoc 2017-04-28 [PMID: 28455345] (WB, Rat)
• Delgado-Maroto Virginia, Benitez Raquel, Forte-Lago Irene et al. Cortistatin reduces atherosclerosis in hyperlipidemic ApoE-deficient mice and the formation of foam cells. Scientific Reports 2017-04-13 [PMID: 28406244] (WB, Mouse)
• Li J, Xiu Z, Wang R et al. The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPARgamma. Life Sci. 2017-05-15 [PMID: 28377266]
• Mailleux J, Vanmierlo T, Bogie JF et al. Active liver X receptor signaling in phagocytes in multiple sclerosis lesions. Mult. Scler. 2017-02-01 [PMID: 28273782]
• Pulakazhi Venu VK, Adijiang A, Seibert T et al. Heat shock protein 27-derived atheroprotection involves reverse cholesterol transport that is dependent on GM-CSF to maintain ABCA1 and ABCG1 expression in ApoE(-/-) mice. FASEB J. 2017-02-23 [PMID: 28232480]
• Cook EC, Nelson JK, Sorrentino V et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017-02-23 [PMID: 28231341]
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017-02-16 [PMID: 28205585] (WB, Mouse)
• Hafiane A, Genest J. ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis Atherosclerosis 2017-01-17 [PMID: 28129550] (WB, Human)
• Dong P, Pan L, Zhang X et al. Hawthorn (Crataegus pinnatifida Bunge) leave flavonoids attenuate atherosclerosis development in apoE knock-out mice J Ethnopharmacol 2017-01-21 [PMID: 28119096] (WB, Mouse)
• Cui X, Chopp M et al. ABCA1/ApoE/HDL Pathway Mediates GW3965-Induced Neurorestoration After Stroke. Stroke 2017-01-02 [PMID: 28028143] (WB, Mouse)
• Nishiga M, Horie T, Kuwabara Y et al. MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol. Circ. Res. 2016-12-05 [PMID: 27920122] (Human)
• Cermak S, Kosicek M, Mladenovic-Djordjevic A et al. Loss of Cathepsin B and L Leads to Lysosomal Dysfunction, NPC-Like Cholesterol Sequestration and Accumulation of the Key Alzheimer's Proteins. PLoS ONE. 2016-11-30 [PMID: 27902765] (WB, Chinese Hamster)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. 2016-11-24 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. 2016-11-15 [PMID: 27862930] (WB, Human)
• Luo L, Yang T. Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med. 2016-10-01 [PMID: 27698729] (Mouse)
• Conlon DM, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest 2016-09-06 [PMID: 27599291] (IB)
• de Gaetano M, Crean D, Barry M, Belton O. M1- and M2-Type Macrophage Responses Are Predictive of Adverse Outcomes in Human Atherosclerosis Front Immunol 2016-08-03 [PMID: 27486460] (WB)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Cui C, Ye X, Chopp M et al. miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats. Stem Cells Transl Med. 2016-07-26 [PMID: 27460851] (IF/IHC, Rat)
• Bechor S, Zolberg Relevy N, Harari A et al. 9-cis beta-Carotene Increased Cholesterol Efflux to HDL in Macrophages. Nutrients 2016-07-19 [PMID: 27447665] (WB)
• Khan F, Ahmed F, Pushparaj PN et al. Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest PLoS ONE 2016-07-22 [PMID: 27441372] (WB, Human)
• Kim SY, Lim EJ, Yoon YS et al. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages Sci Rep 2016-07-13 [PMID: 27406916]
• Zinkhan EK, Zalla JM, Carpenter JR et al. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats Physiol Rep 2016-07-01 [PMID: 27401460]
• Hnatiuk AP, Ong SG, Olea FD et al. Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-alpha (HIF1-alpha) in an Ovine Model of Acute Myocardial Infarction. J Am Heart Assoc 2016-07-06 [PMID: 27385426] (WB, Mouse)
• Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 (IHC-Fr, Mouse)
  
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• Hsieh J, Koseki M, Molusky MM et al. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis Nature 2016-07-14 [PMID: 27383786]
• Kemmerer M, Wittig I, Richter F et al. AMPK activates LXR alpha and ABCA1 expression in human macrophages. Int. J. Biochem. Cell Biol. 2016-06-22 [PMID: 27343431] (WB)
• Ollikainen E, Tulamo R, Lehti S et al. Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture. J. Neuropathol. Exp. Neurol. 2016-07-01 [PMID: 27283327] (ICC/IF)
• Westerterp M, Tsuchiya K et al. Deficiency of ATP-Binding Cassette Transporters A1 and G1 in Endothelial Cells Accelerates Atherosclerosis in Mice. Arterioscler Thromb Vasc Biol 2016-01-07 [PMID: 27199450] (WB, Mouse)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016-04-22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016-04-10 [PMID: 27063951] (WB, Mouse)
• Wolfe AR, Bambhroliya A, Reddy JP et al. MiR-33a Decreases High-Density Lipoprotein-Induced Radiation Sensitivity in Breast Cancer. Int. J. Radiat. Oncol. Biol. Phys. 2016-06-01 [PMID: 27055396] (WB, Human)
• Shrestha E, Hussein MA, Savas JN et al. PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J. Biol. Chem. 2016-03-29 [PMID: 27026705] (WB, Mouse)
• Hong L, Xu X, Huang J et al. Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency Sci Rep 2016-02-08 [PMID: 26852751] (IHC-P, Porcine)
• Schafer-Somi S, Palme N. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability Reprod. Domest. Anim. 2016-02-05 [PMID: 26848092] (ICC/IF, Canine)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep 2016-01-29 [PMID: 26821916] (WB, Mouse)
• Fournier N, Tardivel S, Benoist JF et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim. Biophys. Acta. 2016-01-08 [PMID: 26776055] (WB, Human, Mouse)
• Merkl M, Ertl R, Handschuh S et al. The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues. Theriogenology 2016-04-01 [PMID: 26711702]
• Schreurs BG, Sparks DL. Dietary high cholesterol and trace metals in the drinking water increase levels of ABCA1 in the rabbit hippocampus and temporal cortex. J Alzheimers Dis 2015-01-01 [PMID: 26444796] (IF/IHC, Rabbit)
• Bisoendial R, Tabet F et al. Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis. Arterioscler Thromb Vasc Biol 2015-01-11 [PMID: 26359513] (IF/IHC, Mouse)
• Hussein MA, Shrestha E, Ouimet M et al. LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2. PLoS ONE. 2015-08-20 [PMID: 26288135] (WB, Mouse)
• Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes 2015-12-01 [PMID: 26253613]
• Ito A, Hong C, Rong X et al. LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling Elife. 2015-07-30 [PMID: 26173179] (WB, Mouse)
• Ramezani A, Dubrovsky L, Pushkarsky T et al. Stimulation of Liver X Receptor Has Potent Anti-HIV Effects in a Humanized Mouse Model of HIV Infection J. Pharmacol. Exp. Ther. 2015-09-01 [PMID: 26126533] (WB, Human)
• Casali BT, Corona AW, Mariani MM et al. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease J. Neurosci. 2015-06-17 [PMID: 26085639] (WB, Mouse)
• Fond AM, Lee CS, Schulman IG et al. Apoptotic cells trigger a membrane-initiated pathway to increase ABCA1. J Clin Invest 2015-07-01 [PMID: 26075824] (WB)
• Baker AH, Watt J, Huang CK et al. Tributyltin Engages Multiple Nuclear Receptor Pathways and Suppresses Osteogenesis in Bone Marrow Multipotent Stromal Cells. Chem. Res. Toxicol. 2015-05-13 [PMID: 25932594] (WB, Mouse)
• Liu Q, Li J, Liang Q et al. Sparstolonin B suppresses rat vascular smooth muscle cell proliferation, migration, inflammatory response and lipid accumulation Vascul. Pharmacol. 2015-04-11 [PMID: 25869499] (WB, Rat)
• Chen Y, Duan Y et al. Inhibition of ERK1/2 and activation of LXR synergistically reduce atherosclerotic lesions in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 2015-01-04 [PMID: 25810299] (IF/IHC, Mouse)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015-02-21 [PMID: 25708389] (WB, Mouse)
• Kuo Cheng-Hsiang, Leon Lorraine, Chung Eun Ji et al. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B Mater Biol Med. 2014-12-14 [PMID: 25685357]
• Park SH, Paek JH, Shin D et al. Purple perilla extracts with alpha-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages Int. J. Mol. Med. 2015-02-12 [PMID: 25673178] (WB, Mouse)
• Chou JL, Huang RL, Shay J et al. Hypermethylation of the TGF-beta target, ABCA1 is associated with poor prognosis in ovarian cancer patients. Clin Epigenetics 2015-01-01 [PMID: 25628764] (IF/IHC)
• Du X M, Kim M J et al. HDL particle size is a critical determinant of ABCA1-mediated macrophage cellular cholesterol export. Circ Res 2015-03-27 [PMID: 25589556] (WB, Human)
• Hasegawa Akinori, Sato Kengo, Shirai Remina et al. Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo. PloS One 2014-01-01 [PMID: 25462164] (WB, Human)
• Hong C, Marshall Sm, Mcdaniel Al et al. The LXR-Idol Axis Differentially Regulates Plasma LDL Levels in Primates and Mice. Cell Metab. 2014-11-04 [PMID: 25440061]
• Dong F, Mo Z, Eid W et al. Akt Inhibition Promotes ABCA1-Mediated Cholesterol Efflux to ApoA-I through Suppressing mTORC1. PLoS OnE. 2014-11-22 [PMID: 25415591] (WB, Hamster)
• Shibuya Y, Chang Cc, Huang Lh et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases AB1-42 Clearance. J. neurosci. 2014-10-22 [PMID: 25339759] (WB, Mouse)
• Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014-09-12 [PMID: 25217640]
• Ahn Sang Bong, Jang Kiseok, Jun Dae Won et al. Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease. Dig Dis Sci. 2014-08-08 [PMID: 25102981] (Human)
• Horie Takahiro, Nishino Tomohiro, Baba Osamu et al. MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. Sci Rep. 2014-06-16 [PMID: 24931346] (Mouse)
• Xu Y, Liu Q, Xu Y et al. Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT. J Lipid Res. 2014-06-07 [PMID: 24908654]
• Chi Yan, Wang Le, Liu Yuanyuan et al. 7-ketocholesteryl-9-carboxynonanoate enhances ATP binding cassette transporter A1 expression mediated by PPARgamma in THP-1 macrophages. Atherosclerosis. 2014-02-15 [PMID: 24814410] (Human)
• Liu W, Jiang J, Yan D et al. Pentraxin 3 promotes oxLDL uptake and inhibits cholesterol efflux from macrophage-derived foam cells. Exp. Mol. Pathol. 2014-03-24 [PMID: 24675235] (WB, Human)
• Sallam T, Ito A, Rong X et al. The macrophage lipopolysaccharide binding protein gene is an LXR target that promotes macrophage survival and atherosclerosis. J. Lipid Res. 2014-03-26 [PMID: 24671012] (WB, Mouse)
• Bocchetta S, Maillard P, Yamamoto M et al. Up-Regulation of the ATP-Binding Cassette Transporter A1 Inhibits Hepatitis C Virus Infection. PLoS ONE 2014-03-20 [PMID: 24646941] (WB, Human)
• Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 2014-03-05 [PMID: 24637412] (WB, Mouse)
• Palme N, Becher A, Merkl M et al. Immunolocalization of the Cholesterol Transporters ABCA1 and ABCG1 in Canine Reproductive Tract Tissues and Spermatozoa. Reprod. Domest. Anim. 2014-03-11 [PMID: 24612239] (ICC/IF, IHC-P, Canine)
• Vinod M, Chennamsetty I, Colin S et al. miR-206 controls LXRa expression and promotes LXR-mediated cholesterol efflux in macrophages. Biochim. Biophys. Acta 2014-03-03 [PMID: 24603323] (WB, Human, Mouse)
• Allahverdian S, Chehroudi AC, McManus BM, Francis GA. Contribution of Intimal Smooth Muscle Cells to Cholesterol Accumulation and Macrophage-Like Cells in Human Atherosclerosis. Circulation 2014-01-31 [PMID: 24481950] (IHC-P, Human)
• Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013-12-04 [PMID: 24371802] (WB, Human)
• Aseem O, Smith BT, Cooley MA et al. Cubilin Maintains Blood Levels of HDL and Albumin. J Am Soc Nephrol 2013-12-19 [PMID: 24357674]
• Li N, Xu Y, Feng T et al. Identification of a Selective Agonist for Liver X Receptor alpha (LXRalpha) via Screening of a Synthetic Compound Library. J Biomol Screen 2013-12-13 [PMID: 24334278] (WB, Mouse, Human)
• Ying R, Yuan Y, Qin YF. The combination of L-4F and simvastatin stimulate cholesterol efflux and related proteins expressions to reduce atherosclerotic lesions in apoE knockout mice. Lipids Health Dis 2013-12-08 [PMID: 24314261] (WB, Mouse)
• Horie T, Nishino T, Baba O et al. MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice. Nat Commun. 2013-12-03 [PMID: 24300912] (WB, Human, Mouse)
• Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013-11-06 [PMID: 24223214] (WB, Mouse)
• Ingram WJ, Crowther LM, Little EB et al. ABC transporter activity linked to radiation resistance and molecular subtype in pediatric medulloblastoma. Exp Hematol Oncol 2013-10-04 [PMID: 24219920] (ICC/IF, Human)
  
  Details:
  This publication used the DL488 conjugated form of this antibody (NB400-105G)
• Ohara K, Wakabayashi H, Taniguchi Y et al. Quercetin-3-O-glucuronide induces ABCA1 expression by LXRalpha activation in murine macrophages. Biochem Biophys Res Commun. 2013-11-29 [PMID: 24216107] (WB, Mouse)
• Pan X, Jiang X C et al. Impaired cholesterol metabolism and enhanced atherosclerosis in clock mutant mice. Circulation 2013-10-15 [PMID: 24014832] (WB, Mouse)
• Baumann M, Korner M, Huang X et al. Placental ABCA1 and ABCG1 expression in gestational disease: Pre-eclampsia affects ABCA1 levels in syncytiotrophoblasts. Placenta 2013-07-20 [PMID: 23880356] (WB, Human)
• Hu Y, Yang Y, Yu Y et al. Synthesis and Identification of New Flavonoids Targeting Liver X Receptor beta Involved Pathway as Potential Facilitators of Abeta Clearance with Reduced Lipid Accumulation. J Med Chem 2013-08-08 [PMID: 23844653] (WB, Mouse)
• Shao F, Ford DA. Differential Regulation of ABCA1 and Macrophage Cholesterol Efflux by Elaidic and Oleic Acids. Lipids 2013-06-26 [PMID: 23800855] (WB, Human)
• A-Gonzalez N, Guillen JA, Gallardo G et al. The nuclear receptor LXRalpha controls the functional specialization of splenic macrophages. Nat Immunol 2013-08-01 [PMID: 23770640] (WB, Human, Mouse)
• Sorrentino V, Nelson JK, Maspero E et al. The LXR-IDOL axis defines a clathrin, caveolae, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation. J Lipid Res 2013-06-03 [PMID: 23733886] (WB, Human)
• Ignatova ID, Angdisen J, Moran E, Schulman IG. Differential Regulation of Gene Expression by LXRs in Response to Macrophage Cholesterol Loading. Mol Endocrinol 2013-05-17 [PMID: 23686114] (WB, Human)
• Pommier AJ, Dufour J, Alves G et al. Liver X Receptors Protect from Development of Prostatic Intra-Epithelial Neoplasia in Mice. PLoS Genet 2013-05-01 [PMID: 23675307] (WB, Mouse)
• Lim HY, Thiam CH, Yeo KP et al. Lymphatic Vessels Are Essential for the Removal of Cholesterol from Peripheral Tissues by SR-BI-Mediated Transport of HDL. Cell Metab 2013-05-07 [PMID: 23663736] (ICC/IF, Mouse)
• Mulay V, Wood P, Manetsch M et al. Inhibition of Mitogen-Activated Protein Kinase Erk1/2 Promotes Protein Degradation of ATP Binding Cassette Transporters A1 and G1 in CHO and HuH7 Cells. PLoS One 2013-04-25 [PMID: 23634230] (WB, Hamster, Human)
• Dufour J, Pommier A, Alves G et al. Lack of Liver X Receptors Leads to Cell Proliferation in a Model of Mouse Dorsal Prostate Epithelial Cell PLoS One 2013-01-01 [PMID: 23554947] (WB, Mouse)
• Beaslas O, Metso J, Nissila E et al. Osbpl8 Deficiency in Mouse Causes an Elevation of High-Density Lipoproteins and Gender-Specific Alterations of Lipid Metabolism PLoS One 2013-01-01 [PMID: 23554939] (IHC-P, WB, Mouse)
• Lyssenko NN, Nickel M, Tang C, Phillips MC. Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability. FASEB J 2013-07-01 [PMID: 23543682] (Human)
• de Aguiar Vallim TQ, Tarling EJ, Kim T et al. MicroRNA-144 regulates hepatic ATP binding cassette transporter A1 and plasma high-density lipoprotein after activation of the nuclear receptor farnesoid X receptor Circ Res. 2013-06-07 [PMID: 23519696] (WB, Mouse)
• Zhang L, Jiang M, Shui Y et al. DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux---an LXR-dependent mechanism. Biochim Biophys Acta 2013-03-01 [PMID: 23466610]
• Lo Sasso G, Bovenga F, Murzilli S et al. Liver x receptors inhibit proliferation of human colorectal cancer cells and growth of intestinal tumors in mice. Gastroenterology 2013-06-01 [PMID: 23419360] (IF/IHC, WB, Human)
• Scotti E, Calamai M, Goulbourne CN et al. IDOL stimulates clathrin-independent endocytosis and MVB-mediated lysosomal degradation of the LDLR. Mol Cell Biol 2013-02-04 [PMID: 23382078] (WB, Human)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013-02-19 [PMID: 23334894] (WB, Mouse)
• Horie T, Baba O, Kuwabara Y et al. MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice. J Am Heart Assoc 2012-01-01 [PMID: 23316322] (WB, Mouse)
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation. Biochem Pharmacol. 2013-07-01 [PMID: 23313547] (WB, Human)
• Liu XY, Lu Q, Ouyang XP et al. Apelin-13 increases expression of ATP-binding cassette transporter A1 via activating protein kinase C alpha signaling in THP-1 macrophage-derived foam cells Atherosclerosis 2013-02-01 [PMID: 23290264] (WB, Human)
• Marquart T J, Wu J et al. Anti-miR-33 therapy does not alter the progression of atherosclerosis in low-density lipoprotein receptor-deficient mice. Arterioscler Thromb Vasc Biol 2013-01-03 [PMID: 23288159] (WB, Mouse)
• Park DW, Lee HK, Lyu JH et al. TLR2 stimulates ABCA1 expression via PKC-n and PLD2 pathway Biochem Biophys Res Commun 2012-12-19 [PMID: 23261454] (WB, Mouse)
• Lee BH, Taylor MG, Robinet P et al. Dysregulation of Cholesterol Homeostasis in Human Prostate Cancer through Loss of ABCA1. Cancer Res 2013-01-23 [PMID: 23233737] (WB, Human)
• Dominguez JA, Xie Y, Dunne WM et al. Intestine-Specific Mttp Deletion Decreases Mortality and Prevents Sepsis-Induced Intestinal Injury in a Murine Model of Pseudomonas aeruginosa Pneumonia PLoS One 2012-01-01 [PMID: 23145105] (WB, Mouse)
• Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012-11-07 [PMID: 23130136]
• Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013-01-01 [PMID: 23117655] (WB, Mouse)
• Cardilo-Reis L, Gruber S, Schreier SM et al. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype EMBO Mol Med 2012-10-01 [PMID: 23027612] (WB, IF/IHC, Mouse)
• Machado-Lima A, Iborra RT, Pinto RS et al. Advanced glycated albumin isolated from poorly controlled type 1 diabetes mellitus patients alters macrophage gene expression impairing ABCA-1 mediated reverse cholesterol transport Diabetes Metab Res Rev 2012-09-26 [PMID: 23015358] (ICC/IF, Human)
• Zhou Q, Mei Y, Shoji T et al. Rho-associated coiled-coil-containing kinase 2 deficiency in bone marrow-derived cells leads to increased cholesterol efflux and decreased atherosclerosis Circulation 2012-10-30 [PMID: 23011471] (WB, Mouse)
• Chen J, Zhang X, Kusumo H et al. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis Biochim Biophys Acta 2012-09-23 [PMID: 23010475] (WB, Rat)
• Peter C, Waibel M, Keppeler H et al. Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1 Autoimmunity 2012-08-23 [PMID: 22913458] (WB, Human)
• Kurano M, Hara M, Tsuneyama K et al. Modulation of lipid metabolism with the overexpression of NPC1L1 in mouse liver J Lipid Res 2012-11-01 [PMID: 22891292]
• Mandrekar-Colucci S, Karlo JC, Landreth GE. Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-gamma-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease J Neurosci 2012-07-25 [PMID: 22836247] (WB, Mouse)
• Machado RM, Nakandakare ER, Quintao ECR et al. Omega-6 polyunsaturated fatty acids prevent atherosclerosis development in LDLr-KO mice, in spite of displaying a pro-inflammatory profile similar to trans fatty acids Atherosclerosis 2012-01-01 [PMID: 22809447] (IHC-Fr, IF/IHC, ICC/IF, Mouse)
• Allen RM, Marquart TJ, Albert CJ et al. miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity EMBO Mol Med 2012-09-01 [PMID: 22767443] (WB, Human)
• Xia X, Jung D, Webb P, Zhang A et al. Liver X receptor beta and peroxisome proliferator-activated receptor delta regulate cholesterol transport in murine cholangiocytes Hepatology 2012-12-01 [PMID: 22729460] (ICC/IF, WB, Rat)
• Noghero A, Perino A, Seano G et al. Liver x receptor activation reduces angiogenesis by impairing lipid raft localization and signaling of vascular endothelial growth factor receptor-2 Arterioscler Thromb Vasc Biol 2012-09-01 [PMID: 22723445] (ICC/IF, Human)
• Luo Z, Li J, Nabar NR, Lin X et al. Efficacy of a Therapeutic Vaccine Using Mutated ?-amyloid Sensitized Dendritic Cells in Alzheimer’s Mice J Neuroimmune Pharmacol 2012-06-09 [PMID: 22684353] (IF/IHC, Human)
• Yasuyo Okada, Kiyomi Ueyama, Jyun-ichi Nishikawa, Masanori Semma, Atsushi Ichikawa. Effect of 6-O-alpha-maltosyl-beta cyclodextrin and its cholesterol inclusion complex on cellular cholesterol levels and ABCA1 and ABCG1 expression in mouse mastocytoma P-815 cells, . Carbohydrate Research, 10.1016/j.carres.2012.04.019. 2012-01-01 [PMID: 22677519] (WB, Mouse)
• Cox JV, Naher N, Abdelrahman YM, BellRJ. Host HDL biogenesis machinery is recruited to the inclusion of Chlamydia trachomatis-infected cells and regulates chlamydial growth Cell Microbiol 2012-06-04 [PMID: 22672264] (ICC/IF, Human)
• Scheffler K, Krohn M, Dunkelmann T, Stenzel J, Miroux B, Ibrahim S, von Bohlen Und Halbach O, Heinze HJ, Walker LC, Gsponer JA, Pahnke J. Mitochondrial DNA polymorphisms specifically modify cerebral ?-amyloid proteostasis. Acta Neuropathol. 2012-04-18 [PMID: 22526016] (WB, Mouse)
• Tang S-L, Chen W-J, Yin K, et el. PAPP-A Negatively Regulates ABCA1, ABCG1 and SR-B1 Expression by inhibiting LXR alpha through the IGF-I-mediated signaling pathway Atherosclerosis 2012-06-01 [PMID: 22503545] (WB, Human)
• Castilho G, Okuda LS, Pinto RS, Iborra RT, Nakandakare ER, Santos CX, Laurindo FR, Passarelli M. ER stress is associated with reduced ABCA-1 protein levels in macrophages treated with advanced glycated albumin - Reversal by a chemical chaperone. Int J Biochem Cell Biol. 2012-04-03 [PMID: 22497927] (ICC/IF, Mouse)
• Iio A, Ohguchi K, Iinuma M, Nozawa Y, Ito M. Hesperetin Upregulates ABCA1 Expression and Promotes Cholesterol Efflux from THP-1 Macrophages. J Nat Prod;75(4):563-6. 2012-04-27 [PMID: 22429094] (WB, Human)
• Morbitzer D, Namgaladze D, Brune B. Phospholipase A(2)-Modified Low-Density Lipoprotein Activates Liver X Receptor in Human Macrophages Cell Biochem Biophys 2012-02-26 [PMID: 22367754] (WB, Human)
• Coleman EM, Walker TN, Hildreth JEK. Loss of Niemann Pick type C proteins 1 and 2 greatly enhances HIV infectivity and is associated with accumulation of HIV Gag and cholesterol in late endosomes/lysosomes. Virology Journal 249(1):3. 2012-01-01 [PMID: 22273177]
• Cui HL, Grant A, Mukhamedova N et al. HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux. Journal of Lipid Research. 2012-01-19 [PMID: 22262807]
• Hossain MA, Ngeth S, Chan T et al. Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I. BMC Biochemistry 1613(1):1. 2012-01-01 [PMID: 22248050]
• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, Mouse)
• McIntosh AL, Atshaves BP, Storey SM et al. Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. Journal of Lipid Research. 2012-01-05 [PMID: 22223861]
• Boadu E, Nelson R, Francis GA. ABCA1-dependent mobilization of lysosomal cholesterol requires functional Niemann-Pick C2 but not Niemann-Pick C1 protein. Biochimica Et Biophysica Acta. 2011-12-10 [PMID: 22179027]
• Kammerer I, Ringseis R, Biemann R et al. 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages. Lipids in Health and Disease 3010(1):222. 2011-11-01 [PMID: 22129452]
• Deleterious impact of elaidic trans fatty acid on ABCA1-mediated cholesterol efflux from mouse and human macrophages. Fournier N, Attia N, Rousseau-Ralliard D et al. Biochim Biophys Acta [PMID: 22074701] (WB, Mouse)
• Kanter JE, Tang C, Oram JF, Bornfeldt KE. Acyl-CoA synthetase 1 is required for oleate and linoleate mediated inhibition of cholesterol efflux through ATP-binding cassette transporter A1 in macrophages. Biochimica Et Biophysica Acta. 2011-10-12 [PMID: 22020260]
• Xu Y, Lai F, Xu Y et al. Mycophenolic acid induces ATP-binding cassette transporter A1 (ABCA1) expression through the PPAR[gamma]-LXR[alpha]-ABCA1 pathway. Biochem Biophys Res Commun;414(4):779-782. 2011-11-04 [PMID: 22005457]
• Le Lay S, Rodriguez M, Jessup W et al. Caveolin-1-mediated apolipoprotein a-I membrane binding sites are not required for cholesterol efflux. PLoS ONE. 2011-01-01 [PMID: 21858084]
• Oligomeric ABCA1 and ApoA-I-Mediated Lipid Removal: Molecular Evidence that ApoA-I Interaction with ABCA1 Activatesthe Phosphatidylcholine Biosynthesis Pathway. J Lipid Res. 2011-08-16 [PMID: 21846716]
• Nagashima M, Watanabe T, Terasaki M et al. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice Diabetologia 2011-10-01 [PMID: 21786155] (WB, Mouse)
• Elali A, Hermann DM. Liver X receptor activation enhances blood-brain barrier integrity in the ischemic brain and increases the abundance of ATP-binding cassette transporters ABCB1 and ABCC1 on brain capillary cells. Brain Pathol. 2011-07-18 [PMID: 21767321]
• Hauff KD, Mitchell RW, Xu FY et al. Mifepristone Treatment Results in Differential Regulation of Glycerolipid Biosynthesis in Baby Hamster Kidney Cells Expressing a Mifepristone-Inducible ABCA1. Lipids;46(9):795-804. 2011-09-01 [PMID: 21710238]
• Lee JY, Karwatsky J, Ma L, Zha X. ABCA1 increases extracellular ATP to mediate cholesterol efflux to apoA-I. Am J Physiol Cell Physiol;301(4):C886-94. 2011-10-01 [PMID: 21697542] (WB, Hamster)
• Zhang J, Kris-Etherton PM, Thompson JT et al. Alpha-linolenic acid increases cholesterol efflux in macrophage-derived foam cells by decreasing stearoyl CoA desaturase 1 expression: evidence for a farnesoid-X-receptor mechanism of action. J Nutr Biochem. 2011-06-07 [PMID: 21658928]
• Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther;338(3):870-8. 2011-09-01 [PMID: 21628419]
• Ting TC, Miyazaki-Anzai S, Masuda M et al. Increased lipogenesis and stearate accelerate vascular calcification in calcifying vascular cells. J Biol Chem;286(27):23938-23949. 2011-07-08 [PMID: 21596756]
• Terwel D, Steffensen KR, Verghese PB et al. Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-{alpha} Expression for Microglial A{beta} Phagocytosis. J Neurosci;31(19):7049-59. 2011-05-11 [PMID: 21562267]
• Nikitina L, Wenger F, Baumann M et al. Expression and localization pattern of ABCA1 in diverse human placental primary cells and tissues. Placenta;32(6):420-30. 2011-06-01 [PMID: 21501868]
• Wong BXW, Kyle RA, Croft KD et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids;46(4):371-80. 2011-04-01 [PMID: 21286835]
• Hong C, Walczak R, Dhamko H et al. Constitutive activation of LXR in macrophages regulates metabolic and inflammatory gene expression: identification of ARL7 as a direct target. J Lipid Res;52(3):531-539. 2011-03-01 [PMID: 21187453]
• Loane DJ, Washington PM, Vardanian L et al. Modulation of ABCA1 by an LXR Agonist Reduces Beta-Amyloid Levels and Improves Outcome after Traumatic Brain Injury. J Neurotrauma. 2011-02-01 [PMID: 21175399]
• Ma L, Dong F, Denis M et al. Ht31, a protein kinase A anchoring inhibitor, induces robust cholesterol efflux and reverses macrophage foam cell formation through ATP-binding cassette transporter A1. J Biol Chem. 2011-02-01 [PMID: 21106522] (WB)
• Law RH, Lukoyanova N, Voskoboinik I et al. The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 2010-11-18 [PMID: 21037563] (WB)
• Jain D, Hebden AK, Nakamura TM et al. HAATI survivors replace canonical telomeres with blocks of generic heterochromatin. Nature 2010-09-09 [PMID: 20829796] (ICC/IF)
• Wang Shu, Wu Dayong, Matthan Nirupa R et al. Enhanced aortic macrophage lipid accumulation and inflammatory response in LDL receptor null mice fed an atherogenic diet. Lipids 2010-01-01 [PMID: 20686867] (WB, Mouse)
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010-08-01 [PMID: 20629037]
• Aye IL, Waddell BJ, Mark PJ, Keelan JA. Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity. Biochim Biophys Acta 2010-09-01 [PMID: 20570635]
• McLaren JE, Michael DR, Salter RC et al. IL-33 reduces macrophage foam cell formation. J Immunol 2010-07-15 [PMID: 20543107] (WB, Human, Mouse)
• Howard AD, Verghese PB, Arrese EL, Soulages JL. Characterization of apoA-I-dependent lipid efflux from adipocytes and role of ABCA1. Mol Cell Biochem 2010-10-01 [PMID: 20535530] (Mouse)
• Yang H, Chen K, Zhang X et al. Vitamin A deficiency results in dysregulation of lipid efflux pathway in rat kidney. Pediatr Nephrol 2010-08-01 [PMID: 20480185] (IHC-P, Rat)
• Thompson PA, Gauthier KC, Varley AW, Kitchens RL. ABCA1 promotes the efflux of bacterial LPS from macrophages and accelerates recovery from LPS-induced tolerance. J Lipid Res 2010-09-01 [PMID: 20472936] (WB)
• J E McLaren, C J Calder, B P McSharry et al. The TNF-Like Protein 1A-Death Receptor 3 Pathway Promotes Macrophage Foam Cell Formation In Vitro. J Immunol; 184 (10): 5827-5834. 2010-01-01 [PMID: 20410491]
• Tancevski I, Demetz E, Eller P et al. The liver-selective thyromimetic T-0681 influences reverse cholesterol transport and atherosclerosis development in mice. PLoS One 2010-01-01 [PMID: 20090943] (Mouse)
• Ouvrier A, Cadet R, Lobaccaro JM et al. LXR regulate cholesterol homeostasis in the proximal mouse epididymis. Folia Histochem Cytobiol 2009-01-01 [PMID: 20067898] (IF/IHC, Mouse)
• Crisafulli C, Di Paola R, Mazzon E et al. Liver X receptor agonist treatment reduced splanchnic ischemia and reperfusion injury. J Leukoc Biol. 2009-12-22 [PMID: 20028773]
• Xue JH, Yuan Z, Wu Y et al. High glucose promotes intracellular lipid accumulation in vascular smooth muscle cells by impairing cholesterol influx and efflux balance. Cardiovasc Res;86(1):141-50. 2010-04-01 [PMID: 20007688] (WB, Human)
• Zhang Y, Yin L, Anderson J, Ma H et al. Identification of novel pathways that control farnesoid X receptor-mediated hypocholesterolemia. J Biol Chem;285(5):3035-43. 2010-01-29 [PMID: 19996107] (WB, Mouse, Rat)
• Scoles DR, Xu X, Wang H et al. Liver X receptor agonist inhibits proliferation of ovarian carcinoma cells stimulated by oxidized low density lipoprotein Gynecol Oncol 2010-01-01 [PMID: 19854496] (WB, Human)
• Tang C, Liu Y, Kessler PS et al. The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor. J Biol Chem;284(47):32336-43. 2009-11-20 [PMID: 19783654] (IP, WB, Hamster)
• Bogan RL, Hennebold JD. The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Reproduction 2010-01-01 [PMID: 19776099] (WB)
• Maitra U, Parks JS, Li L. An innate immunity signaling process suppresses macrophage ABCA1 expression through IRAK-1-mediated downregulation of retinoic acid receptor alpha and NFATc2. Mol Cell Biol;29(22):5989-97. 2009-11-01 [PMID: 19752193]
• Larrede S, Quinn C M et al. Stimulation of cholesterol efflux by LXR agonists in cholesterol-loaded human macrophages is ABCA1-dependent but ABCG1-independent. Arterioscler Thromb Vasc Biol 2009-01-11 [PMID: 19729607] (WB, Human)
• Welch C, Santra MK, El-Assaad W et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472CAN-0009-0128. [PMID: 19706769]
• Wang Shu, Wu Dayong, Lamon-Fava Stefania et al. In vitro fatty acid enrichment of macrophages alters inflammatory response and net cholesterol accumulation. The British Journal of Nutrition 2009-01-01 [PMID: 19660150] (WB, Human)
• Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res;105(5):500-510. 2009-01-01 [PMID: 19644050]
• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Cell Physiol 2009-08-01 [PMID: 19625677] (WB, Human)
• Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009-01-01 [PMID: 19590047] (FLOW, WB, Mouse)
• Ronis MJ et al. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr;139(8):1431-8. 2009-08-01 [PMID: 19515742] (Chemotaxis, Rat)
• Ouvrier A, Cadet R, Vernet P et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res :M800657-JLR800200. 2009-01-01 [PMID: 19395734]
• Bujold K, Rhainds D, Jossart C et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res :cvp118. 2009-01-01 [PMID: 19377069]
• Sirek AS, Liu L, Naples M et al. Insulin stimulates the expression of Carbohydrate Response Element Binding Protein (ChREBP) by attenuating the repressive effect of POU homeodomain protein Oct-1. Endocrinology :en2008-1702. 2009-01-01 [PMID: 19359385]
• Kim HJ, Moradi H, Yuan J et al. Renal mass reduction results in accumulation of lipids dysregulation of lipid regulatory proteins in the remnant kidney. Am J Physiol Renal Physiol 90761.92008. [PMID: 19357177]
• Maekawa M, Kikuchi J, Kotani K et al. A novel missense mutation of ABCA1 in transmembrane alpha-helix in a Japanese patient with Tangier disease. Atherosclerosis 2009-09-01 [PMID: 19344898] (ICC/IF, Human)
• Duncan KG, Hosseini K, Bailey KR et al. Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) scavenger receptor BI (SR-BI) in the retina retinal pigment epithelium. Br J Ophthalmol;93(8):1116-1120. 2009-01-01 [PMID: 19304587]
• Bhatnagar S, Damron HA, Hillgartner FB. Fibroblast Growth Factor-19, a Novel Factor that Inhibits Hepatic Fatty Acid Synthesis. J Biol Chem;284(15):10023-33. 2009-04-10 [PMID: 19233843]
• Hans CP, Zerfaoui M, Naura AS et al. Thieno[2,3-c]Isoquinolin-5-one, a Potent Poly(ADP-Ribose) Polymerase Inhibitor, Promotes Atherosclerotic Plaque Regression in High-Fat Diet-Fed Apolipoprotein E-Deficient Mice: Effects on Inflammatory Markers Lipid Content. J Pharmacol Exp Ther;329(1):150-158. 2009-01-01 [PMID: 19124646]
• Dang H, Liu Y, Pang W et al. Suppression of 2,3-Oxidosqualene Cyclase by High Fat Diet Contributes to Liver X Receptor-{alpha}-mediated Improvement of Hepatic Lipid Profile. J Biol Chem;284(10):6218-6226. 2009-01-01 [PMID: 19119143]
• Pelletier RM, Yoon SR, Akpovi CD et al. Defects in the regulatory clearance mechanisms favor the breakdown of self-tolerance during spontaneous autoimmune orchitis. Am J Physiol Regulatory Integrative Comp Physiol;296(3):R743-762. 2009-01-01 [PMID: 19052317] (WB, Mustelid)
• Ni S, Ma L, Denis M et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res;50(3):456-66. 2009-03-01 [PMID: 18941142] (ICC/IF, Hamster)
• Pou J, Rebollo A, Roglans N, Sanchez RM, Vazquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008-12-01 [PMID: 18849545]
• Robichaud JC, Francis GA, Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem 2008-12-19 [PMID: 18842588]
• Delvecchio CJ, Bilan P, Nair P et al. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol;295(5):L949-57. 2008-11-01 [PMID: 18820007]
• Nakanishi S, Vikstedt R, Soderlund S et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res;50(2):183-92. 2009-02-01 [PMID: 18787236]
• Cartagena CM, Ahmed F, Burns MP, Pajoohesh-Ganji A, Pak DT, Faden AI, Rebeck GW. Cortical injury increases cholesterol 24S hydroxylase (Cyp46) levels in the rat brain. J Neurotrauma;25(9):1087-98. 2008-09-01 [PMID: 18729719]
• Peng D, Hiipakka RA, Dai Q, Guo J, Reardon CA, Getz GS, Liao S. Antiatherosclerotic effects of a novel synthetic tissue-selective steroidal liver X receptor agonist in low-density lipoprotein receptor-deficient mice. J Pharmacol Exp Ther;327(2):332-42. 2008-11-01 [PMID: 18723776]
• Mukhamedova N, Escher G, D'Souza W, Tchoua U, Grant A, Krozowski Z, Bukrinsky M, Sviridov D. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res;49(11):2312-22. 2008-11-01 [PMID: 18622028] (WB, IP, Mouse)
• Paul V, Meyer HH, Leidl K, Soumian S, Albrecht C. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res;49(10):2259-67. 2008-10-01 [PMID: 18541924] (WB, ELISA, Human)
• Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol;295(1):C242-8. 2008-07-01 [PMID: 18508910]
• Kumar R, McClain D, Young R et al. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease affects PrPC PrPSc concentrations in cultured cells. J Gen Virol;89(6):1525-1532. 2008-01-01 [PMID: 18474570]
• Bowden K, Ridgway ND. OSBP negatively regulates ABCA1 protein stability. J Biol Chem;283(26):18210-7. 2008-06-27 [PMID: 18450749] (WB, Mouse)
• Tancevski I, Wehinger A, Demetz E, Eller P, Duwensee K, Huber J, Hochegger K, Schgoer W, Fievet C, Stellaard F, Rudling M, Patsch JR, Ritsch A. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology;149(7):3708-12. 2008-07-01 [PMID: 18388200]
• Ouimet M, Wang M-D, Cadotte N et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol;28(6):1144-1150. 2008-01-01 [PMID: 18369155] (WB)
• Rode S, Rubic T, Lorenz RL et al. alpha-Tocopherol disturbs macrophage LXRalpha regulation of ABCA1/G1 and cholesterol handling. Biochem Biophys Res Commun 2008-05-09 [PMID: 18328805] (WB, Human)
• Smoak K, Madenspacher J, Jeyaseelan S, Williams B, Dixon D, Poch KR, Nick JA, Worthen GS, Fessler MB. Effects of liver X receptor agonist treatment on pulmonary inflammation and host defense. J Immunol;180(5):3305-12. 2008-03-01 [PMID: 18292555]
• Henry-Berger J, Mouzat K, Baron S, Bernabeu C, Marceau G, Saru JP, Sapin V, Lobaccaro JM, Caira F. Endoglin (CD105) expression is regulated by the liver X receptor alpha (NR1H3) in human trophoblast cell line JAR. Biol Reprod;78(6):968-75. 2008-06-01 [PMID: 18276933]
• Montoudis A, Seidman E, Boudreau F et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008-05-01 [PMID: 18235139] (WB)
• Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, Sato K, Hongob S, Nose K, Ota H, Kobayashi Y, Katagiri T, Shichiri M, Miyazaki A. Impact of salusin-alpha and -beta on human macrophage foam cell formation and coronary atherosclerosis. Circulation;117(5):638-48. 2008-02-05 [PMID: 18212280]
• Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem;283(18):12595-603. 2008-05-02 [PMID: 18211896]
• Zhu M, Fu Y, Hou Y et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol;28(3):527-33. 2008-03-01 [PMID: 18096827] (WB)
• Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem;283(4):2147-55. 2008-01-25 [PMID: 18042552]
• Wang N, Yvan-Charvet L, Lutjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J;22(4):1073-82. 2008-04-01 [PMID: 18039927]
• Zhou X et al. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 283(4):2129-38. 2008-01-25 [PMID: 18029360]
• Yan D, Mayranpaa MI, Wong J, Perttila J, Lehto M, Jauhiainen M, Kovanen PT, Ehnholm C, Brown AJ, Olkkonen VM. OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem;283(1):332-40. 2008-01-04 [PMID: 17991739]
• Out, R et al. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res;102(1):113-20. 2008-01-04 [PMID: 17967783]
• Hoe, H et al. The Metalloprotease Inhibitor TIMP-3 Regulates Amyloid Precursor Protein Apolipoprotein E Receptor Proteolysis. J Neurosci27: 10895-10905. 2007-10-01 [PMID: 17913923]
• Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008-03-01 [PMID: 17884990] (IF/IHC, Rat)
• Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007-12-01 [PMID: 17823458] (WB, Chicken)
• Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation;116(12):1404-12. 2007-09-18 [PMID: 17724261]
• Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007-11-01 [PMID: 17656736]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007-10-01 [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007-10-01 [PMID: 17609524]
• Zelcer N, Khanlou N, Clare R et al. Attenuation of neuroinflammation and Alzheimer's disease pathology by liver x receptors. Proc Natl Acad Sci U S A 2007-06-19 [PMID: 17563384] (Mouse)
• Sporstol M, Mousavi SA, Eskild W et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol;8:5. 2007-01-22 [PMID: 17241464]
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006-01-01 [PMID: 16807312] (WB, Porcine)
• Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006-01-01 [PMID: 16537455] (IF/IHC, Mouse)
• Dong J, Liu J, Lou B et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res;47(6):1307-14. 2006-06-01 [PMID: 16508036]
• Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006-01-01 [PMID: 16492740]
• Duong PT et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res 47:832-843. 2006-01-01 [PMID: 16418537]
• Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006-03-01 [PMID: 16410462]
• Takata Y, Chu V, Collins AR et al. Transcriptional repression of ATP-binding cassette transporter A1 gene in macrophages: a novel atherosclerotic effect of angiotensin II. Circ Res 2005-10-28 [PMID: 16224068] (WB, Mouse)
• Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006-01-01 [PMID: 16077083]
• Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005-08-01 [PMID: 15961705] (WB, Human, Rat)
• Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005-01-01 [PMID: 15890646] (WB, Human)
• Ragozin S, Niemeier A, Laatsch A et al. Knockdown of hepatic ABCA1 by RNA interference decreases plasma HDL cholesterol levels and influences postprandial lipemia in mice. Arterioscler Thromb Vasc Biol 2005-07-01 [PMID: 15845910]
• Makowski L, Brittingham KC, Reynolds JM et al. The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem 2005-04-01 [PMID: 15684432] (EMSA, Mouse)
• Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004-01-01 [PMID: 15578089]
• Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005-01-01 [PMID: 15557325] (WB, Mouse)
• Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005-01-01 [PMID: 15499044]
• O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation;110(18):2881-8. 2004-11-02 [PMID: 15492319]
• Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004-12-01 [PMID: 15469992]
• Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004-01-01 [PMID: 15358760] (WB, Human)
• Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004-01-01 [PMID: 15280376] (IP, Human)
• Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004-10-01 [PMID: 15210833]
• Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004-01-01 [PMID: 15148325] (WB, Human)
• Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004-08-01 [PMID: 15039140]
• Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004-01-01 [PMID: 14993246] (WB, Rat)
• Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004-01-01 [PMID: 14993242] (WB, Human)
• Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004-01-01 [PMID: 14754908] (IP, Human)
• Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004-03-01 [PMID: 14734645]
• Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004-01-01 [PMID: 14701824] (IP, Human)
• Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003-01-01 [PMID: 14673117] (ICC/IF, Mouse, Human)
• Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004-01-01 [PMID: 14660648] (IP, Human)
• Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003-01-01 [PMID: 14529830] (WB, Mouse)
• Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol 2003-12-01 [PMID: 12952856]
• Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003-01-01 [PMID: 12909583] (WB, Human)
• Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003-08-01 [PMID: 12897148] (WB, Mouse)
• Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003-01-01 [PMID: 12890677] (WB, Mouse)
• Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003-01-01 [PMID: 12777468] (WB, Mouse)
• Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003-01-01 [PMID: 12754274] (WB, Mouse)
• Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003-01-01 [PMID: 12754201] (WB, Mouse)
• Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003-01-01 [PMID: 12586329] (IHC-P, Mouse)
• Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003-01-01 [PMID: 12576511] (WB, Mouse)
• Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003-01-01 [PMID: 12547833] (WB, IF/IHC, ICC/IF, Rat)
• Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003-01-01 [PMID: 12531903] (WB, Hamster, Human)
• Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003-01-01 [PMID: 12511593] (WB, IP, Human)
• Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003-01-01 [PMID: 12507911] (WB, Mouse)
• Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002-01-01 [PMID: 12454270] (IP, Human)
• Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol;22(11):1877-83. 2002-11-01 [PMID: 12426219]
• Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002-01-01 [PMID: 12384498] (WB, Human, Mouse)
• Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002-01-01 [PMID: 12215451] (WB, Mouse)
• Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002-01-01 [PMID: 12091489] (WB, Human)
• Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002-01-01 [PMID: 12050168] (WB, Human, Mouse)

Reviews for ABCA1 Antibody (NB400-105) (7)

Average Rating: 4.3

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Verified Customer WB Human 08/15/2021

Summary

• Application: Western Blot
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: AM-5

Comments

• Comments: 10 ug of total protein. 7,5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4ºC. Secondary 1:5000 HRP 1h RT.

reviewed by: Jahangir Iqbal WB Mouse 11/16/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: AF-2

reviewed by: Verified Customer WB Mouse 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23519696
• Species: Mouse

reviewed by: Angeliki Chroni IF Human 11/03/2014

Summary

• Application: Immunofluorescence
• Sample Tested: SK-N-SH human neuroblastoma cells
• Species: Human

reviewed by: Verified Customer WB Rat 10/15/2012

Summary

• Application: Western Blot
• Sample Tested: Cardiomyocytes from newborn rats
• Species: Rat

reviewed by: Cynthia Hong WB Mouse 09/27/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Verified Customer WB Other 06/15/2009

Summary

• Application: Western Blot
• Sample Tested: Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
• Species: Other

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
   • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
2. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
   • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
3. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
   • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
4. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
   • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
5. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
   • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
6. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
   • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
7. Does this product contain any BSA?
   • No, this antibody does not have any BSA in its formulation.
8. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
   • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
9. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
   • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
10. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
    • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
11. Can i use DTT instead of Beta- mercaptoethanol?
    • Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
12. Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
    • We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.
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Bioinformatics

• Gene Symbol: ABCA1
• Entrez:
  • 19(Human)
  • 11303(Mouse)
  • 313210(Rat)
• Uniprot:
  • O95477(Human)