ABCA1 Antibody

Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Western Blot: ABCA1 Antibody [NB400-105] - Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high cholesterol diet were analyzed by western blotting with antibodies against ABCA1, LDLR and ACTIN as a loading control (left panel), quantification of ABCA1 and LDLR protein accumulation levels (right panel). * p<0.05, *** p<0.001 in Student's t test. Error bars represent the +/- mean SEM.. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003483), licensed under a CC-BY licence.

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b regulates ABCA1 and SREBP-1. Western blotting analysis for ABCA1, SREBP-1, CPT1a, and AMPKalpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are shown. TF2B and beta-actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. Representative image of ovarian cancer showing high (left panel) and low (right panel) ABCA1 expression on the cell membrane or cytoplasm (x400). Image collected and cropped by CiteAb from the following publication (//www.clinicalepigeneticsjournal.com/content/7/1/1), licensed under a CC-BY licence.

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using ABCA1 Antibody NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ mice. Representative images are shown. beta-actin was used as the loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep05312), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

• Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, Rb
• Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ABCA1 Antibody Summary

• Immunogen: Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
• Localization: Astrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry 1:400
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • Dual RNAscope ISH-IHC
  • Gel Supershift Assay
  • Knockdown Validated
  • PCR
• Application Notes: This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). Use in immunoblotting reported in scientific literature (PMID: 27599291). Use in blocking/ neutralizing reported in scientific literature (PMID: 30821416). Use in PCR reported in scientific literature (PMID: 27406916). Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation (PMID 19515742) and ELISA (PMID 18541924) were also reported in scientific literature. Knockdown validation (PMID: 31666189). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Charge.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raw 264.7 Whole Cell - T0901317 treated Assay Kit

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA1 Antibody

• ABC1
• ABC-1
• ABC1ATP-binding cassette transporter A1
• ABCA1
• ATP-binding cassette 1
• ATP-binding cassette transporter 1
• ATP-binding cassette, sub-family A (ABC1), member 1
• CERP
• Cholesterol efflux regulatory protein
• EC 2.7.7.8
• EC 2.8.1.8
• EC 3.6.3
• EC 3.6.3.41
• FLJ14958
• HDLDT1
• membrane-bound
• MGC164864
• MGC165011
• TGD
• TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA1 Antibody (NB400-105)(325)

Publications using NB400-105

• Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. Mar 27 2020 [PMID: 32292343] (WB, Rat)
• Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. May 4 2020 [PMID: 32097464] (Flow, Mouse)
• Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy Jan 16 2020 [PMID: 31944172]
• Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. Dec 18 2019 [PMID: 31864712] (WB, Rat)
• Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. Nov 14 2019 [PMID: 31771811] (WB, Mouse)
• Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. Nov 25 2019 [PMID: 31769014]
• Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med Nov 21 2019 [PMID: 31766415] (WB, Human)
• Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. Nov 19 2019 [PMID: 31744820] (WB, Human, Mouse)
• Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019 Oct 27 [PMID: 31666189] (ICC/IF, WB, KD, Human, Mouse)
• Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules Oct 18 2019 [PMID: 31635211] (WB, Mouse)
• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
• Hennessy EJ, van Solingen C, Scacalossi KR et al. The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Nat Metab Jan 1 2019 [PMID: 31410392] (WB, Human)
• Zhou Y, Bazick H, Miles JR et al. A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic mice Exp. Neurol. Aug 3 2019 [PMID: 31386828] (WB, IHC-Fr, Mouse)
• Paul A, Lydic TA, Hogan R, Goo YH Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells Int J Mol Sci Aug 2 2019 [PMID: 31382484] (WB, Mouse)
• Mukhamedova N, Hoang A, Dragoljevic D et al. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog. Jul 1 2019 [PMID: 31344124] (ICC/IF, Human, Mouse)
• Koyama S, Horie T, Nishino T et al. Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice J Am Heart Assoc Jul 2 2019 [PMID: 31242815] (IHC-Fr, Mouse)
• Rege SD, Luiz R, Tsai B et al. Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame it on Fructose? Mol Nutr Food Res May 13 2019 [PMID: 31087499] (WB, Rat)
• Melendez-Avalos A, Sainz-Espunes T, Castrillon-Rivera LE et al. PMP22 regulates cholesterol trafficking and ABCA1-mediated cholesterol efflux J. Neurosci. May 6 2019 [PMID: 31061090] (WB, Mouse)
• Tiwari P, Blank A, Cui C, Schoenfelt KQ et al Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer. J Exp Med. 2019 Jun 3 [PMID: 31053611] (Flow, Human)
  
  Details:
  Citation using the DyLight 650 version of this antibody.
• Liao S, Zhou Q, Zhang Y. Elastic aortic wrap reduced aortic stiffness by partially alleviating the impairment of cholesterol efflux capacity in pigs J Diabetes Metab Disord Aug 7 2018 [PMID: 30918842] (WB, Porcine)
• Storti F, Klee K, Todorova V et al. Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration Elife Mar 13 2019 [PMID: 30864945] (WB, ICC/IF, Mouse)
• Margaryan NV, Hazard-Jenkins H, Salkeni MA et al. The Stem Cell Phenotype of Aggressive Breast Cancer Cells Cancers (Basel) Mar 8 2019 [PMID: 30857267] (IHC, Human)
• Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) Mar 1 2019 [PMID: 30821416] (B/N, Mouse)
• Tang S, Tabet F, Cochran BJ et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle Sci Rep Feb 4 2019 [PMID: 30718702] (WB, Human)
• Vaidya M, Jentsch JA, Peters S et al. Regulation of ABCA1-mediated cholesterol efflux by sphingosine-1-phosphate signalling in macrophages. J. Lipid Res. Jan 17 2019 [PMID: 30655318] (WB, Mouse)
• Fournier N, Attia N, Rousseau-Ralliard D et al. Deleterious impact of elaidic trans fatty acid on ABCA1-mediated cholesterol efflux from mouse and human macrophages. Biochim Biophys Acta. 2011 Oct 12.
• Kuo CH, Leon L, Chung EJ, Huang RT. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. Journal of Materials Chemistry B. 2014 Sep 05
• Nakazeki F, Tsuge I, Horie T et al. MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons Clin. Sci. Feb 28 2019 [PMID: 30777884] (WB, Human)
• Ramon-Vazquez A, de la Rosa JV, Tabraue C et al. Common and Differential Transcriptional Actions of Nuclear Receptors LXRa and LXRb in macrophages. Mol. Cell. Biol. Jan 2 2019 [PMID: 30602495] (WB, Mouse)
• Moon SH, Huang CH, Houlihan SL et al. p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression Cell Dec 14 2018 [PMID: 30580964] (WB, Human)
• Torres-Adorno AM, Vitrac H, Qi Y et al. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux. Oncogene. Nov 20 2018 [PMID: 30459358] (Human)
• Zinkhan EK, Yu B, McKnight R. Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet. Reprod Sci. Nov 19 2018 [PMID: 30453824] (Rat)
• Han Q, Yeung SC, Ip MSM, Mak JCW. Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model. Biochem. Pharmacol. Nov 9 2018 [PMID: 30428911] (WB, Rat)
• O'Reilly ME, Kajani S, Ralston JC et al. Nutritionally Derived Metabolic Cues Typical of the Obese Microenvironment Increase Cholesterol Efflux Capacity of Adipose Tissue Macrophages. Mol Nutr Food Res. Nov 9 2018 [PMID: 30411491] (WB, Mouse)
• Jin P, Bian Y, Wang K et al. Homocysteine accelerates atherosclerosis via inhibiting LXRa-mediated ABCA1/ABCG1-dependent cholesterol efflux from macrophages Life Sciences Dec 1 2018 [PMID: 30393020] (WB, Human)
• Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019 Jan [PMID: 30389579] (Mouse)
• Zhong S, Li L, Zhang YL et al. Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. J. Clin. Invest. Oct 30 2018 [PMID: 30375985] (WB, Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Liao S, Wu H, Chen R. Apolipoprotein A1 mimetic peptide ATI-5261 reverses arterial stiffness at late pregnancy and early postpartum in a COMT-/- mouse model of preeclampsia. Clin Hypertens Sep 15 2018 [PMID: 30237900] (WB, Mouse)
• de Neergaard R, Nielsen JE, Jorgensen A et al. Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology Sep 4 2018 [PMID: 30182437] (IHC, Human)
• Wang X, Luo J, Li N et al. E3317 promotes cholesterol efflux in macrophage cells via enhancing ABCA1 expression. Biochem. Biophys. Res. Commun. Sep 26 2018 [PMID: 30173890] (WB, Human)
• Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International Jul 12 2018 [PMID: 30105261] (WB, Rabbit)
• Lyssenko NN, Haider N, Picataggi A et al. Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium J. Lipid Res. Aug 3 2018 [PMID: 30076206] (IHC, WB, Human)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human)
• Chernick D, Ortiz-Valle S, Jeong A et al. HDL Mimetic Peptide 4F Mitigates AB-Induced Inhibition of ApoE Secretion and Lipidation in Primary Astrocytes and Microglia. J. Neurochem. Jul 20 2018 [PMID: 30028014] (WB, Mouse)
• Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018 Jun 15 [PMID: 29906247] (Mouse)
• Zinkhan EK, Yu B, Schlegel A. Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1 Front Physiol May 29 2018 [PMID: 29896121] (WB, Rat)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-derived Macrophages Mol Nutr Food Res May 26 2018 [PMID: 29802792] (WB, Human)
• Feng T, Liu P, Wang X et al. SIRT1 activator E1231 protects from experimental atherosclerosis and lowers plasma cholesterol and triglycerides by enhancing ABCA1 expression Atherosclerosis Apr 30 2018 [PMID: 29787963] (WB, Mouse)
• Ishigami M, OgaSawara F, Nagao K et al. Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation. Sci Rep Apr 18 2018 [PMID: 29670126] (WB, Human)
• Xie Z, Wang X, Liu X et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc. Mar 3 2018 [PMID: 29502100] (WB, Mouse)
• Ahn SB, Jun DW, Jang K et al. Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease. Korean J Intern Med 2018 Feb 23 [PMID: 29466845]
• Casali BT, Reed-Geaghan EG, Landreth GE. Nuclear receptor agonist-driven modification of inflammation and amyloid pathology enhances and sustains cognitive improvements in a mouse model of Alzheimer's disease. J Neuroinflammation. 2018 Feb 15 [PMID: 29448961] (WB, Mouse)
• Sallam T, Jones M, Thomas BJ, Wu X. Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat. Med. 2018 Feb 12 [PMID: 29431742] (WB)
• Li Zhiqiang, Kabir Inamul, Tietelman Gladys et al. Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death & Disease 2018 Feb 7 [PMID: 29415989] (WB, Mouse)
• Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor‑kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017 Oct 31 [PMID: 29375683] (WB, Mouse)
• Milligan S, Martin GG, Landrock D et al. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice. Biochim Biophys Acta Mol Cell Biol Lipids 2018 Mar [PMID: 29307784] (Mouse)
• Widenmaier SB, Snyder NA, Nguyen TB et al. NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis Cell. 2017 Nov 16 [PMID: 29149604] (WB)
• Doran AC, Ozcan L, Cai B et al. CAMKIIg suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J. Clin. Invest. 2017 Nov 01 [PMID: 28972541] (WB)
• Storti F, Raphael G, Griesser V et al. Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells Exp. Eye Res. 2017 Sep 21 [PMID: 28943268] (ICC/IF, Human)
• Pelletier RM, Akpovi CD, Chen L et al. Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes Am J Physiol Endocrinol Metab. 2017 Dec 31 [PMID: 28851737] (WB, Mouse)
• Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human)
• Liang Y, Li X, Zhang Y et al. Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells Attenuate Cigarette Smoke-Induced Cardiac Remodeling and Dysfunction Front Pharmacol 2017 Aug 14 [PMID: 28804458] (WB, Rat)
• Morizawa YM, Hirayama Y, Ohno N et al. Reactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway Nat Commun 2017 Jun 22 [PMID: 28642575] (IHC-FR, Mouse)
• Wang D, Tosevska A, Heib EH et al. Bilirubin Decreases Macrophage Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Protein Expression J Am Heart Assoc 2017 Apr 28 [PMID: 28455345] (WB, Rat)
• Delgado-Maroto Virginia, Benitez Raquel, Forte-Lago Irene et al. Cortistatin reduces atherosclerosis in hyperlipidemic ApoE-deficient mice and the formation of foam cells. Scientific Reports 2017 Apr 13 [PMID: 28406244] (WB, Mouse)
• Li J, Xiu Z, Wang R et al. The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPARgamma. Life Sci. May 15 2017 [PMID: 28377266]
• Mailleux J, Vanmierlo T, Bogie JF et al. Active liver X receptor signaling in phagocytes in multiple sclerosis lesions. Mult. Scler. Feb 1 2017 [PMID: 28273782]
• Pulakazhi Venu VK, Adijiang A, Seibert T et al. Heat shock protein 27-derived atheroprotection involves reverse cholesterol transport that is dependent on GM-CSF to maintain ABCA1 and ABCG1 expression in ApoE(-/-) mice. FASEB J. 2017 Feb 23 [PMID: 28232480]
• Cook EC, Nelson JK, Sorrentino V et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017 Feb 23 [PMID: 28231341]
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017 Feb 16 [PMID: 28205585] (WB, Mouse)
• Hafiane A, Genest J. ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis Atherosclerosis Jan 17 2017 [PMID: 28129550] (WB, Human)
• Dong P, Pan L, Zhang X et al. Hawthorn (Crataegus pinnatifida Bunge) leave flavonoids attenuate atherosclerosis development in apoE knock-out mice J Ethnopharmacol Jan 21 2017 [PMID: 28119096] (WB, Mouse)
• Nishiga M, Horie T, Kuwabara Y et al. MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol. Circ. Res. Dec 5 2016 [PMID: 27920122] (Human)
• Cermak S, Kosicek M, Mladenovic-Djordjevic A et al. Loss of Cathepsin B and L Leads to Lysosomal Dysfunction, NPC-Like Cholesterol Sequestration and Accumulation of the Key Alzheimer's Proteins. PLoS ONE. Nov 30 2016 [PMID: 27902765] (WB, Chinese Hamster)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)
• Luo L, Yang T. Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med. Oct 1 2016 [PMID: 27698729] (Mouse)
• Conlon Dm, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J. Clin. Invest. Sep 6 2016 [PMID: 27599291] (IB)
• de Gaetano M, Crean D, Barry M, Belton O. M1- and M2-Type Macrophage Responses Are Predictive of Adverse Outcomes in Human Atherosclerosis Front Immunol 2016 Aug 03 [PMID: 27486460] (WB)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Cui C, Ye X, Chopp M et al. miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats. Stem Cells Transl Med. Jul 26 2016 [PMID: 27460851] (IHC, Rat)
• Bechor S, Zolberg Relevy N, Harari A et al. 9-cis beta-Carotene Increased Cholesterol Efflux to HDL in Macrophages. Nutrients 2016 Jul 19 [PMID: 27447665] (WB)
• Khan F, Ahmed F, Pushparaj PN et al. Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest PLoS ONE 2016 Jul 22 [PMID: 27441372] (WB, Human)
• Kim SY, Lim EJ, Yoon YS et al. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages Sci Rep 2016 Jul 13 [PMID: 27406916]
• Zinkhan EK, Zalla JM, Carpenter JR et al. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats Physiol Rep 2016 Jul 01 [PMID: 27401460]
• Hnatiuk AP, Ong SG, Olea FD et al. Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-a (HIF1-a) in an Ovine Model of Acute Myocardial Infarction J Am Heart Assoc 2016 Jul 01 [PMID: 27385426] (WB, Mouse)
• Hsieh J, Koseki M, Molusky MM et al. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis Nature 2016 Jul 14 [PMID: 27383786]
• Kemmerer M, Wittig I, Richter F et al. AMPK activates LXR alpha and ABCA1 expression in human macrophages. Int. J. Biochem. Cell Biol. Jun 22 2016 [PMID: 27343431] (WB)
• Ollikainen E, Tulamo R, Lehti S et al. Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture. J. Neuropathol. Exp. Neurol. Jul 1 2016 [PMID: 27283327] (ICC/IF)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID: 27063951] (WB, Mouse)
• Wolfe AR, Bambhroliya A, Reddy JP et al. MiR-33a Decreases High-Density Lipoprotein-Induced Radiation Sensitivity in Breast Cancer. Int. J. Radiat. Oncol. Biol. Phys. Jun 1 2016 [PMID: 27055396] (WB, Human)
• Shrestha E, Hussein MA, Savas JN et al. PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J. Biol. Chem. 2016 Mar 29 [PMID: 27026705] (WB, Mouse)
• Hong L, Xu X, Huang J et al. Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency Sci Rep Feb 8 2016 [PMID: 26852751] (IHC-P, Porcine)
• Schafer-Somi S, Palme N. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability Reprod. Domest. Anim. Feb 5 2016 [PMID: 26848092] (ICC/IF, Canine)
• Fond AM, Lee CS, Schulman IG et al. Apoptotic cells trigger a membrane-initiated pathway to increase ABCA1. J Clin Invest 2015 Jul 1 [PMID: 26075824] (WB)
• Xia J, Wang W, Xia X et al. Triptolide suppresses secretion of inflammatory factors and promotes expression of ABCA1 International Journal of Clinical and Experimental Medicine Jun 30 2019 (WB, Mouse)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)
• Fournier N, Tardivel S, Benoist JF et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim. Biophys. Acta. 2016 Jan 08 [PMID: 26776055] (WB, Human, Mouse)
• Merkl M, Ertl R, Handschuh S et al. The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues. Theriogenology 2016 Apr 1 [PMID: 26711702]
• Schreurs BG, Sparks DL. Dietary high cholesterol and trace metals in the drinking water increase levels of ABCA1 in the rabbit hippocampus and temporal cortex. J Alzheimers Dis 2015 [PMID: 26444796] (IHC, Rabbit)
• Hussein MA, Shrestha E, Ouimet M et al. LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2. PLoS ONE. 2015 Aug 20 [PMID: 26288135] (WB, Mouse)
• Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes 2015 Dec [PMID: 26253613]
• Ito A, Hong C, Rong X et al. LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling Elife. 2015 Jul 30 [PMID: 26173179] (WB, Mouse)
• Ramezani A, Dubrovsky L, Pushkarsky T et al. Stimulation of Liver X Receptor Has Potent Anti-HIV Effects in a Humanized Mouse Model of HIV Infection J. Pharmacol. Exp. Ther. 2015 Sep 01 [PMID: 26126533] (WB, Human)
• Casali BT, Corona AW, Mariani MM et al. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease J. Neurosci. 2015 Jun 17 [PMID: 26085639] (WB, Mouse)
• Baker AH, Watt J, Huang CK et al. Tributyltin Engages Multiple Nuclear Receptor Pathways and Suppresses Osteogenesis in Bone Marrow Multipotent Stromal Cells. Chem. Res. Toxicol. 2015 May 13 [PMID: 25932594] (WB, Mouse)
• Liu Q, Li J, Liang Q et al. Sparstolonin B suppresses rat vascular smooth muscle cell proliferation, migration, inflammatory response and lipid accumulation Vascul. Pharmacol. 2015 Apr 11 [PMID: 25869499] (WB, Rat)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015 Feb 21 [PMID: 25708389] (WB, Mouse)
• Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of LXR-activating nanoparticle formulations in primary mouse macrophages bioRxiv Aug 25 2019 (WB, Mouse)
• Kuo Cheng-Hsiang, Leon Lorraine, Chung Eun Ji et al. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B Mater Biol Med. 2014 Dec 14 [PMID: 25685357]
• Park SH, Paek JH, Shin D et al. Purple perilla extracts with alpha-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages Int. J. Mol. Med. 2015 Feb 12 [PMID: 25673178] (WB, Mouse)
• Chou JL, Huang RL, Shay J et al. Hypermethylation of the TGF-beta target, ABCA1 is associated with poor prognosis in ovarian cancer patients. Clin Epigenetics 2015 [PMID: 25628764] (IHC)
• Hasegawa Akinori, Sato Kengo, Shirai Remina et al. Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo. PloS One 2014 [PMID: 25462164] (WB, Human)
• Hong C, Marshall Sm, Mcdaniel Al et al. The LXR-Idol Axis Differentially Regulates Plasma LDL Levels in Primates and Mice. Cell Metab. 2014 Nov 04 [PMID: 25440061]
• Dong F, Mo Z, Eid W et al. Akt Inhibition Promotes ABCA1-Mediated Cholesterol Efflux to ApoA-I through Suppressing mTORC1. PLoS OnE. 2014 Nov 22 [PMID: 25415591] (WB, Hamster)
• Shibuya Y, Chang Cc, Huang Lh et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases AB1-42 Clearance. J. neurosci. 2014 Oct 22 [PMID: 25339759] (WB, Mouse)
• Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014 Sep 12 [PMID: 25217640]
• Ahn Sang Bong, Jang Kiseok, Jun Dae Won et al. Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease. Dig Dis Sci. 2014 Aug 08 [PMID: 25102981] (Human)
• Horie Takahiro, Nishino Tomohiro, Baba Osamu et al. MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. Sci Rep. 2014 Jun 16 [PMID: 24931346] (Mouse)
• Xu Y, Liu Q, Xu Y et al. Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT. J Lipid Res. 2014 Jun 7 [PMID: 24908654]
• Chi Yan, Wang Le, Liu Yuanyuan et al. 7-ketocholesteryl-9-carboxynonanoate enhances ATP binding cassette transporter A1 expression mediated by PPARgamma in THP-1 macrophages. Atherosclerosis. 2014 Feb 15 [PMID: 24814410] (Human)
• Liu W, Jiang J, Yan D et al. Pentraxin 3 promotes oxLDL uptake and inhibits cholesterol efflux from macrophage-derived foam cells. Exp. Mol. Pathol. 3/24/2014 [PMID: 24675235] (WB, Human)
• Sallam T, Ito A, Rong X et al. The macrophage lipopolysaccharide binding protein gene is an LXR target that promotes macrophage survival and atherosclerosis. J. Lipid Res. 3/26/2014 [PMID: 24671012] (WB, Mouse)
• Bocchetta S, Maillard P, Yamamoto M et al. Up-Regulation of the ATP-Binding Cassette Transporter A1 Inhibits Hepatitis C Virus Infection. PLoS ONE 3/20/2014 [PMID: 24646941] (WB, Human)
• Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 3/5/2014 [PMID: 24637412] (WB, Mouse)
• Palme N, Becher A, Merkl M et al. Immunolocalization of the Cholesterol Transporters ABCA1 and ABCG1 in Canine Reproductive Tract Tissues and Spermatozoa. Reprod. Domest. Anim. 3/11/2014 [PMID: 24612239] (IHC-P, ICC/IF, Canine)
• Vinod M, Chennamsetty I, Colin S et al. miR-206 controls LXRa expression and promotes LXR-mediated cholesterol efflux in macrophages. Biochim. Biophys. Acta 3/3/2014 [PMID: 24603323] (WB, Human, Mouse)
• Allahverdian S, Chehroudi AC, McManus BM, Francis GA. Contribution of Intimal Smooth Muscle Cells to Cholesterol Accumulation and Macrophage-Like Cells in Human Atherosclerosis. Circulation 2014 Jan 31 [PMID: 24481950] (IHC-P, Human)
• Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013 Dec 4 [PMID: 24371802] (WB, Human)
• Aseem O, Smith BT, Cooley MA et al. Cubilin Maintains Blood Levels of HDL and Albumin. J Am Soc Nephrol 2013 Dec 19 [PMID: 24357674]
• Li N, Xu Y, Feng T et al. Identification of a Selective Agonist for Liver X Receptor alpha (LXRalpha) via Screening of a Synthetic Compound Library. J Biomol Screen 2013 Dec 13 [PMID: 24334278] (WB, Mouse, Human)
• Ying R, Yuan Y, Qin YF. The combination of L-4F and simvastatin stimulate cholesterol efflux and related proteins expressions to reduce atherosclerotic lesions in apoE knockout mice. Lipids Health Dis 2013 Dec 8 [PMID: 24314261] (WB, Mouse)
• Horie T, Nishino T, Baba O et al. MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice. Nat Commun. 2013 Dec 3 [PMID: 24300912] (WB, Human, Mouse)
• Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013 Nov 6 [PMID: 24223214] (WB, Mouse)
• Ingram WJ, Crowther LM, Little EB et al. ABC transporter activity linked to radiation resistance and molecular subtype in pediatric medulloblastoma. Exp Hematol Oncol 2013 Oct 4 [PMID: 24219920] (ICC/IF, Human)
  
  Details:
  This publication used the DL488 conjugated form of this antibody (NB400-105G)
• Ohara K, Wakabayashi H, Taniguchi Y et al. Quercetin-3-O-glucuronide induces ABCA1 expression by LXRalpha activation in murine macrophages. Biochem Biophys Res Commun. 2013 Nov 29 [PMID: 24216107] (WB, Mouse)
• Baumann M, Korner M, Huang X et al. Placental ABCA1 and ABCG1 expression in gestational disease: Pre-eclampsia affects ABCA1 levels in syncytiotrophoblasts. Placenta 2013 Jul 20 [PMID: 23880356] (WB, Human)
• Hu Y, Yang Y, Yu Y et al. Synthesis and Identification of New Flavonoids Targeting Liver X Receptor beta Involved Pathway as Potential Facilitators of Abeta Clearance with Reduced Lipid Accumulation. J Med Chem 2013 Aug 8 [PMID: 23844653] (WB, Mouse)
• Shao F, Ford DA. Differential Regulation of ABCA1 and Macrophage Cholesterol Efflux by Elaidic and Oleic Acids. Lipids 2013 Jun 26 [PMID: 23800855] (WB, Human)
• A-Gonzalez N, Guillen JA, Gallardo G et al. The nuclear receptor LXRalpha controls the functional specialization of splenic macrophages. Nat Immunol 2013 Aug [PMID: 23770640] (WB, Human, Mouse)
• Sorrentino V, Nelson JK, Maspero E et al. The LXR-IDOL axis defines a clathrin, caveolae, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation. J Lipid Res 2013 Jun 3 [PMID: 23733886] (WB, Human)
• Ignatova ID, Angdisen J, Moran E, Schulman IG. Differential Regulation of Gene Expression by LXRs in Response to Macrophage Cholesterol Loading. Mol Endocrinol 2013 May 17 [PMID: 23686114] (WB, Human)
• Pommier AJ, Dufour J, Alves G et al. Liver X Receptors Protect from Development of Prostatic Intra-Epithelial Neoplasia in Mice. PLoS Genet 2013 May [PMID: 23675307] (WB, Mouse)
• Lim HY, Thiam CH, Yeo KP et al. Lymphatic Vessels Are Essential for the Removal of Cholesterol from Peripheral Tissues by SR-BI-Mediated Transport of HDL. Cell Metab 2013 May 7 [PMID: 23663736] (ICC/IF, Mouse)
• Mulay V, Wood P, Manetsch M et al. Inhibition of Mitogen-Activated Protein Kinase Erk1/2 Promotes Protein Degradation of ATP Binding Cassette Transporters A1 and G1 in CHO and HuH7 Cells. PLoS One 2013 Apr 25 [PMID: 23634230] (WB, Hamster, Human)
• Dufour J, Pommier A, Alves G et al. Lack of Liver X Receptors Leads to Cell Proliferation in a Model of Mouse Dorsal Prostate Epithelial Cell PLoS One 2013 [PMID: 23554947] (WB, Mouse)
• Beaslas O, Metso J, Nissila E et al. Osbpl8 Deficiency in Mouse Causes an Elevation of High-Density Lipoproteins and Gender-Specific Alterations of Lipid Metabolism PLoS One 2013 [PMID: 23554939] (WB, IHC-P, Mouse)
• Lyssenko NN, Nickel M, Tang C, Phillips MC. Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability. FASEB J 2013 Jul [PMID: 23543682] (Human)
• de Aguiar Vallim TQ, Tarling EJ, Kim T et al. MicroRNA-144 regulates hepatic ATP binding cassette transporter A1 and plasma high-density lipoprotein after activation of the nuclear receptor farnesoid X receptor Circ Res. 2013 Jun 7 [PMID: 23519696] (WB, Mouse)
• Zhang L, Jiang M, Shui Y et al. DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux---an LXR-dependent mechanism. Biochim Biophys Acta 2013 Mar 1 [PMID: 23466610]
• Lo Sasso G, Bovenga F, Murzilli S et al. Liver x receptors inhibit proliferation of human colorectal cancer cells and growth of intestinal tumors in mice. Gastroenterology 2013 Jun [PMID: 23419360] (WB, IHC, Human)
• Scotti E, Calamai M, Goulbourne CN et al. IDOL stimulates clathrin-independent endocytosis and MVB-mediated lysosomal degradation of the LDLR. Mol Cell Biol 2013 Feb 4 [PMID: 23382078] (WB, Human)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013 Feb 19 [PMID: 23334894] (WB, Mouse)
• Horie T, Baba O, Kuwabara Y et al. MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice. J Am Heart Assoc 2012 [PMID: 23316322] (WB, Mouse)
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation Biochem Pharmacol 2013 Jan 9 [PMID: 23313547] (WB, Human)
• Liu XY, Lu Q, Ouyang XP et al. Apelin-13 increases expression of ATP-binding cassette transporter A1 via activating protein kinase C alpha signaling in THP-1 macrophage-derived foam cells Atherosclerosis 2013 Feb [PMID: 23290264] (WB, Human)
• Park DW, Lee HK, Lyu JH et al. TLR2 stimulates ABCA1 expression via PKC-n and PLD2 pathway Biochem Biophys Res Commun 2012 Dec 19 [PMID: 23261454] (WB, Mouse)
• Lee BH, Taylor MG, Robinet P et al. Dysregulation of Cholesterol Homeostasis in Human Prostate Cancer through Loss of ABCA1. Cancer Res 2013 Jan 23 [PMID: 23233737] (WB, Human)
• Dominguez JA, Xie Y, Dunne WM et al. Intestine-Specific Mttp Deletion Decreases Mortality and Prevents Sepsis-Induced Intestinal Injury in a Murine Model of Pseudomonas aeruginosa Pneumonia PLoS One 2012 [PMID: 23145105] (WB, Mouse)
• Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013 Jan [PMID: 23117655] (WB, Mouse)
• Cardilo-Reis L, Gruber S, Schreier SM et al. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype EMBO Mol Med 2012 Oct [PMID: 23027612] (WB, IHC, Mouse)
• Machado-Lima A, Iborra RT, Pinto RS et al. Advanced glycated albumin isolated from poorly controlled type 1 diabetes mellitus patients alters macrophage gene expression impairing ABCA-1 mediated reverse cholesterol transport Diabetes Metab Res Rev 2012 Sep 26 [PMID: 23015358] (ICC/IF, Human)
• Zhou Q, Mei Y, Shoji T et al. Rho-associated coiled-coil-containing kinase 2 deficiency in bone marrow-derived cells leads to increased cholesterol efflux and decreased atherosclerosis Circulation 2012 Oct 30 [PMID: 23011471] (WB, Mouse)
• Chen J, Zhang X, Kusumo H et al. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis Biochim Biophys Acta 2012 Sep 23 [PMID: 23010475] (WB, Rat)
• Peter C, Waibel M, Keppeler H et al. Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1 Autoimmunity 2012 Aug 23 [PMID: 22913458] (WB, Human)
• Kurano M, Hara M, Tsuneyama K et al. Modulation of lipid metabolism with the overexpression of NPC1L1 in mouse liver J Lipid Res 2012 Nov [PMID: 22891292]
• Mandrekar-Colucci S, Karlo JC, Landreth GE. Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-gamma-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease J Neurosci 2012 Jul 25 [PMID: 22836247] (WB, Mouse)
• Machado RM, Nakandakare ER, Quintao ECR et al. Omega-6 polyunsaturated fatty acids prevent atherosclerosis development in LDLr-KO mice, in spite of displaying a pro-inflammatory profile similar to trans fatty acids Atherosclerosis 2012 [PMID: 22809447] (IHC, IHC-Fr, ICC/IF, Mouse)
• Allen RM, Marquart TJ, Albert CJ et al. miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity EMBO Mol Med 2012 Sep [PMID: 22767443] (WB, Human)
• Xia X, Jung D, Webb P, Zhang A et al. Liver X receptor beta and peroxisome proliferator-activated receptor delta regulate cholesterol transport in murine cholangiocytes Hepatology 2012 Dec [PMID: 22729460] (ICC/IF, WB, Rat)
• Noghero A, Perino A, Seano G et al. Liver x receptor activation reduces angiogenesis by impairing lipid raft localization and signaling of vascular endothelial growth factor receptor-2 Arterioscler Thromb Vasc Biol 2012 Sep [PMID: 22723445] (ICC/IF, Human)
• Luo Z, Li J, Nabar NR, Lin X et al. Efficacy of a Therapeutic Vaccine Using Mutated ?-amyloid Sensitized Dendritic Cells in Alzheimer’s Mice J Neuroimmune Pharmacol 2012 Jun 9 [PMID: 22684353] (IHC, Human)
• Yasuyo Okada, Kiyomi Ueyama, Jyun-ichi Nishikawa, Masanori Semma, Atsushi Ichikawa. Effect of 6-O-alpha-maltosyl-beta cyclodextrin and its cholesterol inclusion complex on cellular cholesterol levels and ABCA1 and ABCG1 expression in mouse mastocytoma P-815 cells,. Carbohydrate Research, 10.1016/j.carres.2012.04.019. 2012 [PMID: 22677519] (WB, Mouse)
• Cox JV, Naher N, Abdelrahman YM, BellRJ. Host HDL biogenesis machinery is recruited to the inclusion of Chlamydia trachomatis-infected cells and regulates chlamydial growth Cell Microbiol 2012 Jun 4 [PMID: 22672264] (ICC/IF, Human)
• Scheffler K, Krohn M, Dunkelmann T, Stenzel J, Miroux B, Ibrahim S, von Bohlen Und Halbach O, Heinze HJ, Walker LC, Gsponer JA, Pahnke J. Mitochondrial DNA polymorphisms specifically modify cerebral ?-amyloid proteostasis. Acta Neuropathol. 2012 Apr 18. [PMID: 22526016] (WB, Mouse)
• Tang S-L, Chen W-J, Yin K, et el. PAPP-A Negatively Regulates ABCA1, ABCG1 and SR-B1 Expression by inhibiting LXR alpha through the IGF-I-mediated signaling pathway Atherosclerosis 2012 Jun [PMID: 22503545] (WB, Human)
• Castilho G, Okuda LS, Pinto RS, Iborra RT, Nakandakare ER, Santos CX, Laurindo FR, Passarelli M. ER stress is associated with reduced ABCA-1 protein levels in macrophages treated with advanced glycated albumin - Reversal by a chemical chaperone. Int J Biochem Cell Biol. 2012 Apr 3. [PMID: 22497927] (ICC/IF, Mouse)
• Iio A, Ohguchi K, Iinuma M, Nozawa Y, Ito M. Hesperetin Upregulates ABCA1 Expression and Promotes Cholesterol Efflux from THP-1 Macrophages. J Nat Prod;75(4):563-6. 2012 Apr 27. [PMID: 22429094] (WB, Human)
• Morbitzer D, Namgaladze D, Brune B. Phospholipase A(2)-Modified Low-Density Lipoprotein Activates Liver X Receptor in Human Macrophages Cell Biochem Biophys 2012 Feb 26 [PMID: 22367754] (WB, Human)
• Coleman EM, Walker TN, Hildreth JEK. Loss of Niemann Pick type C proteins 1 and 2 greatly enhances HIV infectivity and is associated with accumulation of HIV Gag and cholesterol in late endosomes/lysosomes. Virology Journal 249(1):3. 2012 Jan. [PMID: 22273177]
• Cui HL, Grant A, Mukhamedova N et al. HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux. Journal of Lipid Research. 2012 Jan 19. [PMID: 22262807]
• Hossain MA, Ngeth S, Chan T et al. Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I. BMC Biochemistry 1613(1):1. 2012 Jan. [PMID: 22248050]
• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012 Apr 15 [PMID: 22241858] (WB, Mouse)
• Dubland JA. Low lysosomal acid lipase activity in smooth muscle cells relative to macrophages provides new insights into foam cell formation in atherosclerosis. Thesis. 2018 (WB, Human)
• McIntosh AL, Atshaves BP, Storey SM et al. Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. Journal of Lipid Research. 2012 Jan 5. [PMID: 22223861]
• Boadu E, Nelson R, Francis GA. ABCA1-dependent mobilization of lysosomal cholesterol requires functional Niemann-Pick C2 but not Niemann-Pick C1 protein. Biochimica Et Biophysica Acta. 2011 Dec 10. [PMID: 22179027]
• Kammerer I, Ringseis R, Biemann R et al. 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages. Lipids in Health and Disease 3010(1):222. 2011 Nov. [PMID: 22129452]
• Kanter JE, Tang C, Oram JF, Bornfeldt KE. Acyl-CoA synthetase 1 is required for oleate and linoleate mediated inhibition of cholesterol efflux through ATP-binding cassette transporter A1 in macrophages. Biochimica Et Biophysica Acta. 2011 Oct 12. [PMID: 22020260]
• Xu Y, Lai F, Xu Y et al. Mycophenolic acid induces ATP-binding cassette transporter A1 (ABCA1) expression through the PPAR[gamma]-LXR[alpha]-ABCA1 pathway. Biochem Biophys Res Commun;414(4):779-782. 2011 Nov 4. [PMID: 22005457]
• Le Lay S, Rodriguez M, Jessup W et al. Caveolin-1-mediated apolipoprotein a-I membrane binding sites are not required for cholesterol efflux. PLoS ONE. 2011 [PMID: 21858084]
• Oligomeric ABCA1 and ApoA-I-Mediated Lipid Removal: Molecular Evidence that ApoA-I Interaction with ABCA1 Activatesthe Phosphatidylcholine Biosynthesis Pathway. J Lipid Res. 2011 Aug 16. [PMID: 21846716]
• Nagashima M, Watanabe T, Terasaki M et al. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice Diabetologia 2011 Oct [PMID: 21786155] (WB, Mouse)
• Elali A, Hermann DM. Liver X receptor activation enhances blood-brain barrier integrity in the ischemic brain and increases the abundance of ATP-binding cassette transporters ABCB1 and ABCC1 on brain capillary cells. Brain Pathol. 2011 Jul 18. [PMID: 21767321]
• Hauff KD, Mitchell RW, Xu FY et al. Mifepristone Treatment Results in Differential Regulation of Glycerolipid Biosynthesis in Baby Hamster Kidney Cells Expressing a Mifepristone-Inducible ABCA1. Lipids;46(9):795-804. 2011 Sep. [PMID: 21710238]
• Lee JY, Karwatsky J, Ma L, Zha X. ABCA1 increases extracellular ATP to mediate cholesterol efflux to apoA-I. Am J Physiol Cell Physiol;301(4):C886-94. 2011 Oct. [PMID: 21697542] (WB, Hamster)
• Zhang J, Kris-Etherton PM, Thompson JT et al. Alpha-linolenic acid increases cholesterol efflux in macrophage-derived foam cells by decreasing stearoyl CoA desaturase 1 expression: evidence for a farnesoid-X-receptor mechanism of action. J Nutr Biochem. 2011 Jun 7. [PMID: 21658928]
• Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther;338(3):870-8. 2011 Sep. [PMID: 21628419]
• Ting TC, Miyazaki-Anzai S, Masuda M et al. Increased lipogenesis and stearate accelerate vascular calcification in calcifying vascular cells. J Biol Chem;286(27):23938-23949. 2011 Jul 8. [PMID: 21596756]
• Terwel D, Steffensen KR, Verghese PB et al. Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-{alpha} Expression for Microglial A{beta} Phagocytosis. J Neurosci;31(19):7049-59. 2011 May 11. [PMID: 21562267]
• Nikitina L, Wenger F, Baumann M et al. Expression and localization pattern of ABCA1 in diverse human placental primary cells and tissues. Placenta;32(6):420-30. 2011 Jun. [PMID: 21501868]
• Wong BXW, Kyle RA, Croft KD et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids;46(4):371-80. 2011 Apr. [PMID: 21286835]
• Hong C, Walczak R, Dhamko H et al. Constitutive activation of LXR in macrophages regulates metabolic and inflammatory gene expression: identification of ARL7 as a direct target. J Lipid Res;52(3):531-539. 2011 Mar. [PMID: 21187453]
• Loane DJ, Washington PM, Vardanian L et al. Modulation of ABCA1 by an LXR Agonist Reduces Beta-Amyloid Levels and Improves Outcome after Traumatic Brain Injury. J Neurotrauma. 2011 Feb. [PMID: 21175399]
• Ma L, Dong F, Denis M et al. Ht31, a protein kinase A anchoring inhibitor, induces robust cholesterol efflux and reverses macrophage foam cell formation through ATP-binding cassette transporter A1. J Biol Chem. Feb. 2011 [PMID: 21106522] (WB)
• Law RH, Lukoyanova N, Voskoboinik I et al. The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 2010 Nov 18 [PMID: 21037563] (WB)
• Jain D, Hebden AK, Nakamura TM et al. HAATI survivors replace canonical telomeres with blocks of generic heterochromatin. Nature 2010 Sep 9 [PMID: 20829796] (ICC/IF)
• Wang Shu, Wu Dayong, Matthan Nirupa R et al. Enhanced aortic macrophage lipid accumulation and inflammatory response in LDL receptor null mice fed an atherogenic diet. Lipids 2010 [PMID: 20686867] (WB, Mouse)
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010 Aug [PMID: 20629037]
• Aye IL, Waddell BJ, Mark PJ et al. Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity Biochim Biophys Acta. 2010 Sep [PMID: 20570635]
• McLaren JE, Michael DR, Salter RC et al. IL-33 reduces macrophage foam cell formation. J Immunol 2010 Jul 15 [PMID: 20543107] (WB, Human, Mouse)
• Howard AD, Verghese PB, Arrese EL, Soulages JL. Characterization of apoA-I-dependent lipid efflux from adipocytes and role of ABCA1. Mol Cell Biochem 2010 Oct [PMID: 20535530] (Mouse)
• Yang H, Chen K, Zhang X et al. Vitamin A deficiency results in dysregulation of lipid efflux pathway in rat kidney. Pediatr Nephrol 2010 Aug [PMID: 20480185] (IHC-P, Rat)
• Thompson PA, Gauthier KC, Varley AW, Kitchens RL. ABCA1 promotes the efflux of bacterial LPS from macrophages and accelerates recovery from LPS-induced tolerance. J Lipid Res 2010 Sep [PMID: 20472936] (WB)
• J E McLaren, C J Calder, B P McSharry et al. The TNF-Like Protein 1A-Death Receptor 3 Pathway Promotes Macrophage Foam Cell Formation In Vitro. J Immunol; 184 (10): 5827-5834. 2010 [PMID: 20410491]
• Tancevski I, Demetz E, Eller P et al. The liver-selective thyromimetic T-0681 influences reverse cholesterol transport and atherosclerosis development in mice. PLoS One 2010 [PMID: 20090943] (Mouse)
• Ouvrier A, Cadet R, Lobaccaro JM et al. LXR regulate cholesterol homeostasis in the proximal mouse epididymis. Folia Histochem Cytobiol 2009 [PMID: 20067898] (IHC, Mouse)
• Crisafulli C, Di Paola R, Mazzon E et al. Liver X receptor agonist treatment reduced splanchnic ischemia and reperfusion injury. J Leukoc Biol. 2009 Dec 22. [PMID: 20028773]
• Xue JH, Yuan Z, Wu Y et al. High glucose promotes intracellular lipid accumulation in vascular smooth muscle cells by impairing cholesterol influx and efflux balance. Cardiovasc Res;86(1):141-50. 2010 Apr 1. [PMID: 20007688] (WB, Human)
• Zhang Y, Yin L, Anderson J, Ma H et al. Identification of novel pathways that control farnesoid X receptor-mediated hypocholesterolemia. J Biol Chem;285(5):3035-43. 2010 Jan 29. [PMID: 19996107] (WB, Mouse, Rat)
• Scoles DR, Xu X, Wang H et al. Liver X receptor agonist inhibits proliferation of ovarian carcinoma cells stimulated by oxidized low density lipoprotein Gynecol Oncol 2010 Jan [PMID: 19854496] (WB, Human)
• Tang C, Liu Y, Kessler PS et al. The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor. J Biol Chem;284(47):32336-43. 2009 Nov 20. [PMID: 19783654] (WB, IP, Hamster)
• Bogan RL, Hennebold JD. The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Reproduction 2010 Jan [PMID: 19776099] (WB)
• Maitra U, Parks JS, Li L. An innate immunity signaling process suppresses macrophage ABCA1 expression through IRAK-1-mediated downregulation of retinoic acid receptor alpha and NFATc2. Mol Cell Biol;29(22):5989-97. 2009 Nov. [PMID: 19752193]
• Welch C, Santra MK, El-Assaad W et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res :0008-5472CAN-0009-0128. 2009 [PMID: 19706769]
• Wang Shu, Wu Dayong, Lamon-Fava Stefania et al. In vitro fatty acid enrichment of macrophages alters inflammatory response and net cholesterol accumulation. The British Journal of Nutrition 2009 [PMID: 19660150] (WB, Human)
• Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res CIRCRESAHA.109.193870. 2009 [PMID: 19644050]
• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Endocrinol Metab 2009 Aug [PMID: 19625677] (WB, Human)
• Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009 [PMID: 19590047] (WB, FLOW, Mouse)
• Ronis MJ, Chen Y, Badeaux J, Badger TM. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr;139(8):1431-8. 2009 Aug. [PMID: 19515742] (ChIP, Rat)
• Ouvrier A, Cadet R, Vernet P et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res :M800657-JLR800200. 2009 [PMID: 19395734]
• Bujold K, Rhainds D, Jossart C et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res :cvp118. 2009 [PMID: 19377069]
• Sirek AS, Liu L, Naples M et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology;150(8):3483-3492. 2009 [PMID: 19359385]
• Kim HJ, Moradi H, Yuan J et al. Renal mass reduction results in accumulation of lipids dysregulation of lipid regulatory proteins in the remnant kidney. Am J Physiol Renal Physiol;296(6):F1297-1306. 2009 [PMID: 19357177]
• Maekawa M, Kikuchi J, Kotani K et al. A novel missense mutation of ABCA1 in transmembrane alpha-helix in a Japanese patient with Tangier disease. Atherosclerosis 2009 Sep [PMID: 19344898] (ICC/IF, Human)
• Duncan KG, Hosseini K, Bailey KR et al. Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) scavenger receptor BI (SR-BI) in the retina retinal pigment epithelium. Br J Ophthalmol;93(8):1116-1120. 2009 [PMID: 19304587]
• Bhatnagar S, Damron HA, Hillgartner FB. Fibroblast Growth Factor-19, a Novel Factor that Inhibits Hepatic Fatty Acid Synthesis. J Biol Chem;284(15):10023-33. 2009 Apr 10. [PMID: 19233843]
• Hans CP, Zerfaoui M, Naura AS et al. Thieno[2,3-c]Isoquinolin-5-one, a Potent Poly(ADP-Ribose) Polymerase Inhibitor, Promotes Atherosclerotic Plaque Regression in High-Fat Diet-Fed Apolipoprotein E-Deficient Mice: Effects on Inflammatory Markers Lipid Content. J Pharmacol Exp Ther;329(1):150-158. 2009 [PMID: 19124646]
• Dang H, Liu Y, Pang W et al. Suppression of 2,3-Oxidosqualene Cyclase by High Fat Diet Contributes to Liver X Receptor-{alpha}-mediated Improvement of Hepatic Lipid Profile. J Biol Chem;284(10):6218-6226. 2009 [PMID: 19119143]
• Pelletier RM, Yoon SR, Akpovi CD et al. Defects in the regulatory clearance mechanisms favor the breakdown of self-tolerance during spontaneous autoimmune orchitis. Am J Physiol Regulatory Integrative Comp Physiol;296(3):R743-762. 2009 [PMID: 19052317] (WB, Mustelid)
• Ni S, Ma L, Denis M et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res;50(3):456-66. 2009 Mar. [PMID: 18941142] (ICC/IF, Hamster)
• Pou J, Rebollo A, Roglans N, Sanchez RM, Vazquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008 Dec. [PMID: 18849545]
• Robichaud JC, Francis GA, Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem;283(51):35496-506. 2008 Dec 19. [PMID: 18842588]
• Delvecchio CJ, Bilan P, Nair P, Capone JP. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol;295(5):L949-57. 2008 Nov. [PMID: 18820007]
• Nakanishi S, Vikstedt R, Soderlund S et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res;50(2):183-92. 2009 Feb. [PMID: 18787236]
• Cartagena CM, Ahmed F, Burns MP, Pajoohesh-Ganji A, Pak DT, Faden AI, Rebeck GW. Cortical injury increases cholesterol 24S hydroxylase (Cyp46) levels in the rat brain. J Neurotrauma;25(9):1087-98. 2008 Sep. [PMID: 18729719]
• Peng D, Hiipakka RA, Dai Q, Guo J, Reardon CA, Getz GS, Liao S. Antiatherosclerotic effects of a novel synthetic tissue-selective steroidal liver X receptor agonist in low-density lipoprotein receptor-deficient mice. J Pharmacol Exp Ther;327(2):332-42. 2008 Nov. [PMID: 18723776]
• Mukhamedova N, Escher G, D'Souza W, Tchoua U, Grant A, Krozowski Z, Bukrinsky M, Sviridov D. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res;49(11):2312-22. 2008 Nov. [PMID: 18622028] (WB, IP, Mouse)
• Paul V, Meyer HH, Leidl K, Soumian S, Albrecht C. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res;49(10):2259-67. 2008 Oct. [PMID: 18541924] (WB, ELISA, Human)
• Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol;295(1):C242-8. 2008 Jul. [PMID: 18508910]
• Kumar R, McClain D, Young R, Carlson GA. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease and affects PrPC and PrPSc concentrations in cultured cells. J Gen Virol;89(Pt 6):1525-32. 2008 Jun. [PMID: 18474570]
• Bowden K, Ridgway ND. OSBP negatively regulates ABCA1 protein stability. J Biol Chem;283(26):18210-7. 2008 Jun 27. [PMID: 18450749] (WB, Mouse)
• Tancevski I, Wehinger A, Demetz E, Eller P, Duwensee K, Huber J, Hochegger K, Schgoer W, Fievet C, Stellaard F, Rudling M, Patsch JR, Ritsch A. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology;149(7):3708-12. 2008 Jul. [PMID: 18388200]
• Ouimet M, Wang M-D, Cadotte N et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol;28(6):1144-1150. 2008 [PMID: 18369155] (WB)
• Rode S, Rubic T, Lorenz RL et al. alpha-Tocopherol disturbs macrophage LXRalpha regulation of ABCA1/G1 and cholesterol handling. Biochem Biophys Res Commun 2008 May 9 [PMID: 18328805] (WB, Human)
• Smoak K, Madenspacher J, Jeyaseelan S, Williams B, Dixon D, Poch KR, Nick JA, Worthen GS, Fessler MB. Effects of liver X receptor agonist treatment on pulmonary inflammation and host defense. J Immunol;180(5):3305-12. 2008 Mar 1. [PMID: 18292555]
• Henry-Berger J, Mouzat K, Baron S, Bernabeu C, Marceau G, Saru JP, Sapin V, Lobaccaro JM, Caira F. Endoglin (CD105) expression is regulated by the liver X receptor alpha (NR1H3) in human trophoblast cell line JAR. Biol Reprod;78(6):968-75. 2008 Jun. [PMID: 18276933]
• Montoudis, A et al. Intestinal fatty acid binding protein regulates mitochonfrion beta-oxidation cholesterol uptake. J Lipid Res; Volume 49: 961-972. January 30 2008. [PMID: 18235139] (WB)
• Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, Sato K, Hongob S, Nose K, Ota H, Kobayashi Y, Katagiri T, Shichiri M, Miyazaki A. Impact of salusin-alpha and -beta on human macrophage foam cell formation and coronary atherosclerosis. Circulation;117(5):638-48. 2008 Feb 5. [PMID: 18212280]
• Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem;283(18):12595-603. 2008 May 2. [PMID: 18211896]
• Zhu M, Fu Y, Hou Y et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol;28(3):527-33. March 1 2008. [PMID: 18096827] (WB)
• Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem;283(4):2147-55. 2008 Jan 25. [PMID: 18042552]
• Wang N, Yvan-Charvet L, Lutjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J;22(4):1073-82. 2008 Apr. [PMID: 18039927]
• Zhou X, He W, Huang Z, Gotto AM Jr, Hajjar DP, Han J. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 283(4):2129-38. 2008 Jan 25. [PMID: 18029360]
• Yan D, Mayranpaa MI, Wong J, Perttila J, Lehto M, Jauhiainen M, Kovanen PT, Ehnholm C, Brown AJ, Olkkonen VM. OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem;283(1):332-40. 2008 Jan 4. [PMID: 17991739]
• Out R, Jessup W, Le Goff W, Hoekstra M, Gelissen IC, Zhao Y, Kritharides L, Chimini G, Kuiper J, Chapman MJ, Huby T, Van Berkel TJ, Van Eck M. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res;102(1):113-20. 2008 Jan 4. [PMID: 17967783]
• Hoe HS, Cooper MJ, Burns MP, Lewis PA, van der Brug M, Chakraborty G, Cartagena CM, Pak DT, Cookson MR, Rebeck GW. The metalloprotease inhibitor TIMP-3 regulates amyloid precursor protein and apolipoprotein E receptor proteolysis. J Neurosci;27(40):10895-905. 2007 Oct 3. [PMID: 17913923]
• Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008 Mar. [PMID: 17884990] (IHC, Rat)
• Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007 Dec. [PMID: 17823458] (WB, Chicken)
• Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation;116(12):1404-12. 2007 Sep 18. [PMID: 17724261]
• Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007 Nov. [PMID: 17656736]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007 Oct. [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007 Oct. [PMID: 17609524]
• Zelcer N, Khanlou N, Clare R et al. Attenuation of neuroinflammation and Alzheimer's disease pathology by liver x receptors. Proc Natl Acad Sci U S A 2007 Jun 19 [PMID: 17563384] (Mouse)
• Sporstol M, Mousavi SA, Eskild W et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol;8:5. 2007 Jan 22. [PMID: 17241464]
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006 [PMID: 16807312] (WB, Porcine)
• Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006 [PMID: 16537455] (IHC, Mouse)
• Dong J, Liu J, Lou B et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res;47(6):1307-14. 2006 Jun. [PMID: 16508036]
• Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006 [PMID: 16492740]
• Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH, Phillips MC. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res;47(4):832-43. 2006 Apr. [PMID: 16418537]
• Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006 Mar. [PMID: 16410462]
• Takata Y, Chu V, Collins AR et al. Transcriptional repression of ATP-binding cassette transporter A1 gene in macrophages: a novel atherosclerotic effect of angiotensin II. Circ Res 2005 Oct 28 [PMID: 16224068] (WB, Mouse)
• Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006 [PMID: 16077083]
• Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005 Aug. [PMID: 15961705] (WB, Human, Rat)
• Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005 [PMID: 15890646] (WB, Human)
• Ragozin S, Niemeier A, Laatsch A et al. Knockdown of hepatic ABCA1 by RNA interference decreases plasma HDL cholesterol levels and influences postprandial lipemia in mice. Arterioscler Thromb Vasc Biol 2005 Jul [PMID: 15845910]
• Makowski L, Brittingham KC, Reynolds JM et al. The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem 2005 Apr 1 [PMID: 15684432] (EMSA, Mouse)
• Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004 [PMID: 15578089]
• Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005 [PMID: 15557325] (WB, Mouse)
• Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005 Jan. [PMID: 15499044]
• O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation;110(18):2881-8. 2004 Nov 2. [PMID: 15492319]
• Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004 Dec. [PMID: 15469992]
• Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004 [PMID: 15358760] (WB, Human)
• Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004 [PMID: 15280376] (IP, Human)
• Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004 Oct. [PMID: 15210833]
• Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004 [PMID: 15148325] (WB, Human)
• Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004 Aug. [PMID: 15039140]
• Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004 [PMID: 14993246] (WB, Rat)
• Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004 [PMID: 14993242] (WB, Human)
• Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004 [PMID: 14754908] (IP, Human)
• Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004 Mar. [PMID: 14734645]
• Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004 [PMID: 14701824] (IP, Human)
• Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003 [PMID: 14673117] (ICC/IF, Mouse, Human)
• Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004 [PMID: 14660648] (IP, Human)
• Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003 [PMID: 14529830] (WB, Mouse)
• Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol;285(6):F1092-9. 2003 Dec. [PMID: 12952856]
• Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003 [PMID: 12909583] (WB, Human)
• Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003Aug. [PMID: 12897148] (WB, Mouse)
• Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003 [PMID: 12890677] (WB, Mouse)
• Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003 [PMID: 12777468] (WB, Mouse)
• Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003 [PMID: 12754274] (WB, Mouse)
• Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003 [PMID: 12754201] (WB, Mouse)
• Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003 [PMID: 12586329] (IHC-P, Mouse)
• Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003 [PMID: 12576511] (WB, Mouse)
• Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003 [PMID: 12547833] (WB, IHC, ICC/IF, Rat)
• Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003 [PMID: 12531903] (WB, Human, Hamster)
• Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003 [PMID: 12511593] (WB, IP, Human)
• Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003 Jan [PMID: 12507911] (WB, Mouse)
• Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002 [PMID: 12454270] (IP, Human)
• Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol;22(11):1877-83. 2002 Nov 1. [PMID: 12426219]
• Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002 [PMID: 12384498] (WB, Mouse, Human)
• Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002 [PMID: 12215451] (WB, Mouse)
• Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002 [PMID: 12091489] (WB, Human)
• Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002 [PMID: 12050168] (WB, Mouse, Human)

Reviews for ABCA1 Antibody (NB400-105) (6)

Average Rating: 4.3

(Based on 6 reviews)

We have 6 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Jahangir Iqbal WB Mouse 11/16/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: AF-2

Blocking

• Blocking Details: Membranes were blocked in 5% BSA in TBST for 1 h at room temperature

Primary Anitbody

• Dilution Ratio: Membranes were immunoblotted with anti-ABCA1 antibody 1:2000 dilution in 2.5% BSA in TBST overnight at 4 oC

Secondary Antibody

• Secondary Description: Membranes were incubated with secondary antibody conjugated to stabilized peroxidase for 1 hour at room temperature
• Secondary Manufacturer Cat#: Thermo Scientific # 32460
• Secondary Concentration: dilution 1:15,000

Details

• Detection Notes: Bands were visualized used chemiluminescence according to the manufacturer’s instructions

Comments

• Comments: Samples were not boiled and 2X BME was added.

reviewed by: Anonymous WB Mouse 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23519696
• Species: Mouse

Blocking

• Blocking Details: See PMID 23519696

Details

• Detection Notes: See PMID 23519696

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23519696

reviewed by: Angeliki Chroni IF Human 11/03/2014

Summary

• Application: Immunofluorescence
• Sample Tested: SK-N-SH human neuroblastoma cells
• Species: Human

reviewed by: Anonymous WB Rat 10/15/2012

Summary

• Application: Western Blot
• Sample Tested: Cardiomyocytes from newborn rats
• Species: Rat

Blocking

• Blocking Details: PBS Milk 5%, 1 hour, room temperature

Primary Anitbody

• Dilution Ratio: 1000 = dilution, 1 hour, room temperature, PBS without tween and 2% Milk

Secondary Antibody

• Secondary Description: goat anti rabbit IgG HRP
• Secondary Manufacturer Cat#: Amersham NA934V
• Secondary Concentration: 3000 diluted

Details

• Detection Notes: ECL super plus Amersham RPN2132

reviewed by: Cynthia Hong WB Mouse 09/27/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Anonymous WB Other 06/15/2009

Summary

• Application: Western Blot
• Sample Tested: Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk w/ TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1/1000, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: anti-Rabbit IgG HRP, Secondary Ab Dilution Ratio: 1/1000

Details

• Detection Notes: Exposure Time: 1 hour, Positive Control: HC/RA, Specific Bands: 200 kDa

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
   • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
2. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
   • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
3. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
   • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
4. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
   • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
5. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
   • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
6. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
   • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
7. Does this product contain any BSA?
   • No, this antibody does not have any BSA in its formulation.
8. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
   • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
9. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
   • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
10. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
    • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
11. Can i use DTT instead of Beta- mercaptoethanol?
    • Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
12. Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
    • We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.
Show All 12 FAQs.

Bioinformatics

• Gene Symbol: ABCA1
• Entrez:
  • 19(Human)
  • 11303(Mouse)
  • 313210(Rat)
• Uniprot:
  • O95477(Human)