Our customer would like to consult something about the ABCA1 antibody with the Cat No. NB400-105. In the datasheet, it showed this protein expressed very low in most cell types. The customer may test the mouse liver tissues, and he doesn’t know the expression level of ABCA1 in this tissue. Is it necessary to induce the expression via LXR treatment to get enough protein expressed? If he doesn’t induce the expression, how much protein loaded was suitable as recommended?

Our own Western blot data for NB400-105 was generated by treating RAW cells with 9-cis-retinoic acid and 22R-hydroxycholesterol, which are both known to induce expression of ABCA1 in macrophages. 40ug of protein was loaded on to the gel in this instance. Unfortunately we do not have any testing data from mouse liver tissues using this particular antibody, however the data for another of our ABCA1 antibodies may be of interest to your customer: NB100-2068. The Western blot data for this product was generated using mouse liver tissue, and a clear band can be seen at the expected molecular weight in wild type tissue. We loaded 30ug of protein when running this Western blot, however, your customer may need to optimise the staining procedure for their sample type.

Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?

Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.

We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?

Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.

I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.

Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.

To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?

The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.

We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?

ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.

Does this product contain any BSA?

No, this antibody does not have any BSA in its formulation.

I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?

The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.

Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?

The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.

Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?

To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.

Can i use DTT instead of Beta- mercaptoethanol?

Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.

Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?

We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.

For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?

For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.

ABCA1 Antibody

Name: ABCA1 Antibody
Brand: Novus Biologicals
SKU: NB400-105
Rating: 4.3 (6 reviews)

(351 Publications)

(6 Reviews)

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Images

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Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Orthogonal Strategies: Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # ...read more

Biological Strategies: Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples ...read more

Biological Strategies: Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior ...read more

Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then ...read more

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.

Western Blot: ABCA1 Antibody [NB400-105] - Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high ...read more

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b regulates ABCA1 and SREBP-1. Analysis for ABCA1, SREBP-1, CPT1a, and AMPK alpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are ...read more

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC ...read more

Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Biological Strategies: Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced ...read more

Western Blot: ABCA1 Antibody [NB400-105] - miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ ...read more

Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbSpecies Glossary
Applications	WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1 mg/ml

Validated by:

Biological Strategies

Orthogonal Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB400-105AF350

Alexa Fluor 405 NB400-105AF405

Alexa Fluor 488 NB400-105AF488

Alexa Fluor 532 NB400-105AF532

Alexa Fluor 594 NB400-105AF594

Alexa Fluor 647 NB400-105AF647

Alexa Fluor 700 NB400-105AF700

Alexa Fluor 750 NB400-105AF750

Allophycocyanin/Cy7 NB400-105APCCY7

Biotin NB400-105B

DyLight 350 NB400-105UV

DyLight 405 NB400-105V

DyLight 488 NB400-105G

DyLight 550 NB400-105R

DyLight 594 NB400-105DL594

DyLight 650 NB400-105C

DyLight 680 NB400-105FR

DyLight 755 NB400-105IR

FITC NB400-105F

HRP NB400-105H

Janelia Fluor 549 NB400-105JF549

Janelia Fluor 646 NB400-105JF646

PE NB400-105PE

PE/Atto594 NB400-105PEATT594

PE/Cy5.5 NB400-105PECY55

PE/Cy7 NB400-105PECY7

100% Guarantee on every product

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB400-105AF350
Alexa Fluor 405	NB400-105AF405
Alexa Fluor 488	NB400-105AF488
Alexa Fluor 532	NB400-105AF532
Alexa Fluor 594	NB400-105AF594
Alexa Fluor 647	NB400-105AF647
Alexa Fluor 700	NB400-105AF700
Alexa Fluor 750	NB400-105AF750
Allophycocyanin/Cy7	NB400-105APCCY7
Biotin	NB400-105B
DyLight 350	NB400-105UV
DyLight 405	NB400-105V
DyLight 488	NB400-105G
DyLight 550	NB400-105R
DyLight 594	NB400-105DL594
DyLight 650	NB400-105C
DyLight 680	NB400-105FR
DyLight 755	NB400-105IR
FITC	NB400-105F
HRP	NB400-105H
Janelia Fluor 549	NB400-105JF549
Janelia Fluor 646	NB400-105JF646
PE	NB400-105PE
PE/Atto594	NB400-105PEATT594
PE/Cy5.5	NB400-105PECY55
PE/Cy7	NB400-105PECY7

ABCA1 Antibody Summary

Immunogen	Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Localization	Astrocyte Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ABCA1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Western Blot 1:500
Chromatin Immunoprecipitation
ELISA
Flow Cytometry 1:400
Immunoblotting
Immunocytochemistry/Immunofluorescence 1:100
Immunohistochemistry 1:200
Immunohistochemistry-Frozen
Immunohistochemistry-Paraffin 1:200
Immunoprecipitation 1:10-1:500
Block/Neutralize
Dual RNAscope ISH-IHC
Gel Supershift Assay
Knockdown Validated
Knockout Validated
PCR

Application Notes

This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). Use in immunoblotting reported in scientific literature (PMID: 27599291). Use in blocking/ neutralizing reported in scientific literature (PMID: 30821416). Use in PCR reported in scientific literature (PMID: 27406916). Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation (PMID 19515742) and ELISA (PMID 18541924) were also reported in scientific literature. Knockdown validation (PMID: 31666189). Knockout validation (PMID: 32273567). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Charge.

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

Raw 264.7 Whole Cell - T0901317 treated Assay Kit

Control Peptide

ABCA1 Protein (NB400-105PEP)

Reviewed Applications

Read 6 Reviews rated 4.3

using
NB400-105 in the following applications:

Western Blot
Immunofluorescence

Publications

Read Publications using
NB400-105 in the following applications:

B/N
1 publication
ChIP
1 publication
ELISA
1 publication
EMSA
1 publication
FLOW
1 publication
Flow
2 publications
IB
1 publication
ICC/IF
20 publications
IHC
16 publications
IHC-FR
1 publication
IHC-Fr
3 publications
IHC-P
7 publications
IP
9 publications
KD
1 publication
KO
1 publication
WB
189 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Immunogen affinity purified

Alternate Names for ABCA1 Antibody

ABC1
ABC-1
ABC1ATP-binding cassette transporter A1
ABCA1
ATP-binding cassette 1
ATP-binding cassette transporter 1
ATP-binding cassette, sub-family A (ABC1), member 1
CERP
Cholesterol efflux regulatory protein
EC 2.7.7.8
EC 2.8.1.8
EC 3.6.3
EC 3.6.3.41
FLJ14958
HDLDT1
membrane-bound
MGC164864
MGC165011
TGD
TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCA1 Antibody (NB400-105)(351)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Canine, Chicken, Chinese Hamster, Hamster, Mustelid, Porcine, Rabbit.

We have publications tested in 16 applications: B/N, ChIP, ELISA, EMSA, FLOW, Flow, IB, ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P, IP, KD, KO, WB.

Showing Publications 1 - 10 of 351. Show All 351 Publications.

Publications using NB400-105	Applications	Species
Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology Dec 14 2020 [PMID: 33381046] (WB, Mouse)	WB	Mouse
Mukherjee V, Ramakrishna P, Bora S, Kotteazeth S Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation Steroids Nov 7 2020 [PMID: 33171131] (WB, Human) Details: Western blot analysis performed on HepG2 cells.	WB	Human
Dodge JC, Jensen EH, Yu J et al. Neutral lipid cacostasis contributes to disease pathogenesis in amyotrophic lateral sclerosis J Neurosci Oct 13 2020 [PMID: 33051352]
Munir MT, Ponce C, Santos JM et al. VD3 and LXR agonist (T0901317) combination demonstrated greater potency in inhibiting cholesterol accumulation and inducing apoptosis via ABCA1-CHOP-BCL-2 cascade in MCF-7 breast cancer cells Mol Biol Rep Oct 1 2020 [PMID: 32990902]
Zhao D, Li J, Xue C et al. TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells J. Biol. Chem. Sep 22 2020 [PMID: 32963108] (Mouse)		Mouse
Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics Aug 13 2020 [PMID: 32929345] (WB, Mouse)	WB	Mouse
Venkat P, Zacharek A, Landschoot-Ward J et al. Exosomes derived from bone marrow mesenchymal stem cells harvested from type two diabetes rats promotes neurorestorative effects after stroke in type two diabetes rats Experimental neurology Sep 2 2020 [PMID: 32889008] (IHC-P, Rat)	IHC-P	Rat
Xia X, Xu Q, Liu M et al. Deubiquitination of CD36 by UCHL1 promotes foam cell formation Cell Death Dis Aug 15 2020 [PMID: 32801299] (IP)	IP
Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research Jul 1 2020 [PMID: 32621955] (WB, Mouse)	WB	Mouse
Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. Jun 30 2020 [PMID: 32602556] (WB, Rat)	WB	Rat
Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. May 8 2020 [PMID: 32437644] (WB, Human)	WB	Human
Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020 May 21 [PMID: 32437392] (WB, Human)	WB	Human
Pathak P, Helsley RN, Brown AL et al. Small Molecule Inhibition of Gut Microbial Choline Trimethylamine Lyase Activity Alters Host Cholesterol and Bile Acid Metabolism Am. J. Physiol. Heart Circ. Physiol. Apr 24 2020 [PMID: 32330092] (WB, Mouse)	WB	Mouse
Hiebl V, Schachner D, Ladurner A et al. Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations Biol Proced Online Apr 11 2020 [PMID: 32308567]
Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. Mar 27 2020 [PMID: 32292343] (WB, Rat)	WB	Rat
He Q, Wang F, Honda T et al. Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation Sci Rep Apr 9 2020 [PMID: 32273567] (KO, Mouse)	KO	Mouse
Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. Feb 28 2020 [PMID: 32173266] (WB, Mouse)	WB	Mouse
Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. May 4 2020 [PMID: 32097464] (Flow, Mouse)	Flow	Mouse
Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy Jan 16 2020 [PMID: 31944172]
Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. Dec 18 2019 [PMID: 31864712] (WB, Rat)	WB	Rat
Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. Nov 14 2019 [PMID: 31771811] (WB, Mouse)	WB	Mouse
Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. Nov 25 2019 [PMID: 31769014]
Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med Nov 21 2019 [PMID: 31766415] (WB, Human)	WB	Human
Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. Nov 19 2019 [PMID: 31744820] (WB, Human, Mouse)	WB	Human, Mouse
Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019 Oct 27 [PMID: 31666189] (ICC/IF, WB, KD, Human, Mouse)	ICC/IF, WB, KD	Human, Mouse
Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules Oct 18 2019 [PMID: 31635211] (WB, Mouse)	WB	Mouse
Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
Hennessy EJ, van Solingen C, Scacalossi KR et al. The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Nat Metab Jan 1 2019 [PMID: 31410392] (WB, Human)	WB	Human
Zhou Y, Bazick H, Miles JR et al. A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic mice Exp. Neurol. Aug 3 2019 [PMID: 31386828] (WB, IHC-Fr, Mouse)	WB, IHC-Fr	Mouse
Paul A, Lydic TA, Hogan R, Goo YH Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells Int J Mol Sci Aug 2 2019 [PMID: 31382484] (WB, Mouse)	WB	Mouse
Mukhamedova N, Hoang A, Dragoljevic D et al. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog. Jul 1 2019 [PMID: 31344124] (ICC/IF, Human, Mouse)	ICC/IF	Human, Mouse
Koyama S, Horie T, Nishino T et al. Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice J Am Heart Assoc Jul 2 2019 [PMID: 31242815] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Rege SD, Luiz R, Tsai B et al. Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame it on Fructose? Mol Nutr Food Res May 13 2019 [PMID: 31087499] (WB, Rat)	WB	Rat
Melendez-Avalos A, Sainz-Espunes T, Castrillon-Rivera LE et al. PMP22 regulates cholesterol trafficking and ABCA1-mediated cholesterol efflux J. Neurosci. May 6 2019 [PMID: 31061090] (WB, Mouse)	WB	Mouse
Tiwari P, Blank A, Cui C, Schoenfelt KQ et al Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer. J Exp Med. 2019 Jun 3 [PMID: 31053611] (Flow, Human) Details: Citation using the DyLight 650 version of this antibody.	Flow	Human
Liao S, Zhou Q, Zhang Y. Elastic aortic wrap reduced aortic stiffness by partially alleviating the impairment of cholesterol efflux capacity in pigs J Diabetes Metab Disord Aug 7 2018 [PMID: 30918842] (WB, Porcine)	WB	Porcine
Storti F, Klee K, Todorova V et al. Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration Elife Mar 13 2019 [PMID: 30864945] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Margaryan NV, Hazard-Jenkins H, Salkeni MA et al. The Stem Cell Phenotype of Aggressive Breast Cancer Cells Cancers (Basel) Mar 8 2019 [PMID: 30857267] (IHC, Human)	IHC	Human
Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) Mar 1 2019 [PMID: 30821416] (B/N, Mouse)	B/N	Mouse
Nakazeki F, Tsuge I, Horie T et al. MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons Clin. Sci. Feb 28 2019 [PMID: 30777884] (WB, Human)	WB	Human
Tang S, Tabet F, Cochran BJ et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle Sci Rep Feb 4 2019 [PMID: 30718702] (WB, Human)	WB	Human
Vaidya M, Jentsch JA, Peters S et al. Regulation of ABCA1-mediated cholesterol efflux by sphingosine-1-phosphate signalling in macrophages. J. Lipid Res. Jan 17 2019 [PMID: 30655318] (WB, Mouse)	WB	Mouse
Ramon-Vazquez A, de la Rosa JV, Tabraue C et al. Common and Differential Transcriptional Actions of Nuclear Receptors LXRa and LXRb in macrophages. Mol. Cell. Biol. Jan 2 2019 [PMID: 30602495] (WB, Mouse)	WB	Mouse
Moon SH, Huang CH, Houlihan SL et al. p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression Cell Dec 14 2018 [PMID: 30580964] (WB, Human)	WB	Human
Torres-Adorno AM, Vitrac H, Qi Y et al. Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux. Oncogene. Nov 20 2018 [PMID: 30459358] (Human)		Human
Zinkhan EK, Yu B, McKnight R. Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet. Reprod Sci. Nov 19 2018 [PMID: 30453824] (Rat)		Rat
Han Q, Yeung SC, Ip MSM, Mak JCW. Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model. Biochem. Pharmacol. Nov 9 2018 [PMID: 30428911] (WB, Rat)	WB	Rat
O'Reilly ME, Kajani S, Ralston JC et al. Nutritionally Derived Metabolic Cues Typical of the Obese Microenvironment Increase Cholesterol Efflux Capacity of Adipose Tissue Macrophages. Mol Nutr Food Res. Nov 9 2018 [PMID: 30411491] (WB, Mouse)	WB	Mouse
Jin P, Bian Y, Wang K et al. Homocysteine accelerates atherosclerosis via inhibiting LXRa-mediated ABCA1/ABCG1-dependent cholesterol efflux from macrophages Life Sciences Dec 1 2018 [PMID: 30393020] (WB, Human)	WB	Human
Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019 Jan [PMID: 30389579] (Mouse)		Mouse
Zhong S, Li L, Zhang YL et al. Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. J. Clin. Invest. Oct 30 2018 [PMID: 30375985] (WB, Mouse)	WB	Mouse
Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)	WB	Mouse
Liao S, Wu H, Chen R. Apolipoprotein A1 mimetic peptide ATI-5261 reverses arterial stiffness at late pregnancy and early postpartum in a COMT-/- mouse model of preeclampsia. Clin Hypertens Sep 15 2018 [PMID: 30237900] (WB, Mouse)	WB	Mouse
de Neergaard R, Nielsen JE, Jorgensen A et al. Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology Sep 4 2018 [PMID: 30182437] (IHC, Human)	IHC	Human
Wang X, Luo J, Li N et al. E3317 promotes cholesterol efflux in macrophage cells via enhancing ABCA1 expression. Biochem. Biophys. Res. Commun. Sep 26 2018 [PMID: 30173890] (WB, Human)	WB	Human
Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International Jul 12 2018 [PMID: 30105261] (WB, Rabbit)	WB	Rabbit
Lyssenko NN, Haider N, Picataggi A et al. Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium J. Lipid Res. Aug 3 2018 [PMID: 30076206] (IHC, WB, Human)	IHC, WB	Human
Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human)	WB	Human
Chernick D, Ortiz-Valle S, Jeong A et al. HDL Mimetic Peptide 4F Mitigates AB-Induced Inhibition of ApoE Secretion and Lipidation in Primary Astrocytes and Microglia. J. Neurochem. Jul 20 2018 [PMID: 30028014] (WB, Mouse)	WB	Mouse
Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018 Jun 15 [PMID: 29906247] (Mouse)		Mouse
Zinkhan EK, Yu B, Schlegel A. Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1 Front Physiol May 29 2018 [PMID: 29896121] (WB, Rat)	WB	Rat
Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-derived Macrophages Mol Nutr Food Res May 26 2018 [PMID: 29802792] (WB, Human)	WB	Human
Feng T, Liu P, Wang X et al. SIRT1 activator E1231 protects from experimental atherosclerosis and lowers plasma cholesterol and triglycerides by enhancing ABCA1 expression Atherosclerosis Apr 30 2018 [PMID: 29787963] (WB, Mouse)	WB	Mouse
Ishigami M, OgaSawara F, Nagao K et al. Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation. Sci Rep Apr 18 2018 [PMID: 29670126] (WB, Human)	WB	Human
Xie Z, Wang X, Liu X et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc. Mar 3 2018 [PMID: 29502100] (WB, Mouse)	WB	Mouse
Ahn SB, Jun DW, Jang K et al. Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease. Korean J Intern Med 2018 Feb 23 [PMID: 29466845]
Casali BT, Reed-Geaghan EG, Landreth GE. Nuclear receptor agonist-driven modification of inflammation and amyloid pathology enhances and sustains cognitive improvements in a mouse model of Alzheimer's disease. J Neuroinflammation. 2018 Feb 15 [PMID: 29448961] (WB, Mouse)	WB	Mouse
Sallam T, Jones M, Thomas BJ, Wu X. Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat. Med. 2018 Feb 12 [PMID: 29431742] (WB)	WB
Li Zhiqiang, Kabir Inamul, Tietelman Gladys et al. Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death & Disease 2018 Feb 7 [PMID: 29415989] (WB, Mouse)	WB	Mouse
Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor‑kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017 Oct 31 [PMID: 29375683] (WB, Mouse)	WB	Mouse
Milligan S, Martin GG, Landrock D et al. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice. Biochim Biophys Acta Mol Cell Biol Lipids 2018 Mar [PMID: 29307784] (Mouse)		Mouse
Widenmaier SB, Snyder NA, Nguyen TB et al. NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis Cell. 2017 Nov 16 [PMID: 29149604] (WB)	WB
Doran AC, Ozcan L, Cai B et al. CAMKIIg suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J. Clin. Invest. 2017 Nov 01 [PMID: 28972541] (WB)	WB
Storti F, Raphael G, Griesser V et al. Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells Exp. Eye Res. 2017 Sep 21 [PMID: 28943268] (ICC/IF, Human)	ICC/IF	Human
Pelletier RM, Akpovi CD, Chen L et al. Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes Am J Physiol Endocrinol Metab. 2017 Dec 31 [PMID: 28851737] (WB, Mouse)	WB	Mouse
Liang Y, Li X, Zhang Y et al. Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells Attenuate Cigarette Smoke-Induced Cardiac Remodeling and Dysfunction Front Pharmacol 2017 Aug 14 [PMID: 28804458] (WB, Rat)	WB	Rat
Morizawa YM, Hirayama Y, Ohno N et al. Reactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway Nat Commun 2017 Jun 22 [PMID: 28642575] (IHC-FR, Mouse)	IHC-FR	Mouse
Wang D, Tosevska A, Heib EH et al. Bilirubin Decreases Macrophage Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Protein Expression J Am Heart Assoc 2017 Apr 28 [PMID: 28455345] (WB, Rat)	WB	Rat
Delgado-Maroto Virginia, Benitez Raquel, Forte-Lago Irene et al. Cortistatin reduces atherosclerosis in hyperlipidemic ApoE-deficient mice and the formation of foam cells. Scientific Reports 2017 Apr 13 [PMID: 28406244] (WB, Mouse)	WB	Mouse
Li J, Xiu Z, Wang R et al. The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPARgamma. Life Sci. May 15 2017 [PMID: 28377266]
Mailleux J, Vanmierlo T, Bogie JF et al. Active liver X receptor signaling in phagocytes in multiple sclerosis lesions. Mult. Scler. Feb 1 2017 [PMID: 28273782]
Pulakazhi Venu VK, Adijiang A, Seibert T et al. Heat shock protein 27-derived atheroprotection involves reverse cholesterol transport that is dependent on GM-CSF to maintain ABCA1 and ABCG1 expression in ApoE(-/-) mice. FASEB J. 2017 Feb 23 [PMID: 28232480]
Cook EC, Nelson JK, Sorrentino V et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017 Feb 23 [PMID: 28231341]
Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017 Feb 16 [PMID: 28205585] (WB, Mouse)	WB	Mouse
Hafiane A, Genest J. ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis Atherosclerosis Jan 17 2017 [PMID: 28129550] (WB, Human)	WB	Human
Dong P, Pan L, Zhang X et al. Hawthorn (Crataegus pinnatifida Bunge) leave flavonoids attenuate atherosclerosis development in apoE knock-out mice J Ethnopharmacol Jan 21 2017 [PMID: 28119096] (WB, Mouse)	WB	Mouse
Cui X, Chopp M et al. ABCA1/ApoE/HDL Pathway Mediates GW3965-Induced Neurorestoration After Stroke. Stroke Jan 2 2017 [PMID: 28028143] (WB, Mouse)	WB	Mouse
Nishiga M, Horie T, Kuwabara Y et al. MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol. Circ. Res. Dec 5 2016 [PMID: 27920122] (Human)		Human
Cermak S, Kosicek M, Mladenovic-Djordjevic A et al. Loss of Cathepsin B and L Leads to Lysosomal Dysfunction, NPC-Like Cholesterol Sequestration and Accumulation of the Key Alzheimer's Proteins. PLoS ONE. Nov 30 2016 [PMID: 27902765] (WB, Chinese Hamster)	WB	Chinese Hamster
Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 [PMID: 27890613] (Mouse)		Mouse
Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)	WB	Human
Luo L, Yang T. Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med. Oct 1 2016 [PMID: 27698729] (Mouse)		Mouse
Conlon Dm, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J. Clin. Invest. Sep 6 2016 [PMID: 27599291] (IB)	IB
de Gaetano M, Crean D, Barry M, Belton O. M1- and M2-Type Macrophage Responses Are Predictive of Adverse Outcomes in Human Atherosclerosis Front Immunol 2016 Aug 03 [PMID: 27486460] (WB)	WB
Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)	WB	Mouse
Bechor S, Zolberg Relevy N, Harari A et al. 9-cis beta-Carotene Increased Cholesterol Efflux to HDL in Macrophages. Nutrients 2016 Jul 19 [PMID: 27447665] (WB)	WB
Khan F, Ahmed F, Pushparaj PN et al. Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest PLoS ONE 2016 Jul 22 [PMID: 27441372] (WB, Human)	WB	Human
Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human)	WB	Human
Cui C, Ye X, Chopp M et al. miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats. Stem Cells Transl Med. Jul 26 2016 [PMID: 27460851] (IHC, Rat)	IHC	Rat
Kim SY, Lim EJ, Yoon YS et al. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages Sci Rep 2016 Jul 13 [PMID: 27406916]
Zinkhan EK, Zalla JM, Carpenter JR et al. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats Physiol Rep 2016 Jul 01 [PMID: 27401460]
Hnatiuk AP, Ong SG, Olea FD et al. Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-a (HIF1-a) in an Ovine Model of Acute Myocardial Infarction J Am Heart Assoc 2016 Jul 01 [PMID: 27385426] (WB, Mouse)	WB	Mouse
Hsieh J, Koseki M, Molusky MM et al. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis Nature 2016 Jul 14 [PMID: 27383786]
Kemmerer M, Wittig I, Richter F et al. AMPK activates LXR alpha and ABCA1 expression in human macrophages. Int. J. Biochem. Cell Biol. Jun 22 2016 [PMID: 27343431] (WB)	WB
Ollikainen E, Tulamo R, Lehti S et al. Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture. J. Neuropathol. Exp. Neurol. Jul 1 2016 [PMID: 27283327] (ICC/IF)	ICC/IF
Westerterp M, Tsuchiya K et al. Deficiency of ATP-Binding Cassette Transporters A1 and G1 in Endothelial Cells Accelerates Atherosclerosis in Mice. Arterioscler Thromb Vasc Biol Jan 7 2016 [PMID: 27199450] (WB, Mouse)	WB	Mouse
Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID: 27114256] (WB, Mouse)	WB	Mouse
Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID: 27063951] (WB, Mouse)	WB	Mouse
Wolfe AR, Bambhroliya A, Reddy JP et al. MiR-33a Decreases High-Density Lipoprotein-Induced Radiation Sensitivity in Breast Cancer. Int. J. Radiat. Oncol. Biol. Phys. Jun 1 2016 [PMID: 27055396] (WB, Human)	WB	Human
Shrestha E, Hussein MA, Savas JN et al. PARP-1 Represses LXR-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J. Biol. Chem. 2016 Mar 29 [PMID: 27026705] (WB, Mouse)	WB	Mouse
Hong L, Xu X, Huang J et al. Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency Sci Rep Feb 8 2016 [PMID: 26852751] (IHC-P, Porcine)	IHC-P	Porcine
Schafer-Somi S, Palme N. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability Reprod. Domest. Anim. Feb 5 2016 [PMID: 26848092] (ICC/IF, Canine)	ICC/IF	Canine
Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)	WB	Mouse
Fournier N, Tardivel S, Benoist JF et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim. Biophys. Acta. 2016 Jan 08 [PMID: 26776055] (WB, Human, Mouse)	WB	Human, Mouse
Merkl M, Ertl R, Handschuh S et al. The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues. Theriogenology 2016 Apr 1 [PMID: 26711702]
Schreurs BG, Sparks DL. Dietary high cholesterol and trace metals in the drinking water increase levels of ABCA1 in the rabbit hippocampus and temporal cortex. J Alzheimers Dis 2015 [PMID: 26444796] (IHC, Rabbit)	IHC	Rabbit
Bisoendial R, Tabet F et al. Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis. Arterioscler Thromb Vasc Biol Jan 11 2015 [PMID: 26359513] (IHC, Mouse)	IHC	Mouse
Hussein MA, Shrestha E, Ouimet M et al. LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2. PLoS ONE. 2015 Aug 20 [PMID: 26288135] (WB, Mouse)	WB	Mouse
Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes 2015 Dec [PMID: 26253613]
Ito A, Hong C, Rong X et al. LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling Elife. 2015 Jul 30 [PMID: 26173179] (WB, Mouse)	WB	Mouse
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Pou J, Rebollo A, Roglans N, Sanchez RM, Vazquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008 Dec. [PMID: 18849545]
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Paul V, Meyer HH, Leidl K, Soumian S, Albrecht C. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res;49(10):2259-67. 2008 Oct. [PMID: 18541924] (WB, ELISA, Human)	WB, ELISA	Human
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Bowden K, Ridgway ND. OSBP negatively regulates ABCA1 protein stability. J Biol Chem;283(26):18210-7. 2008 Jun 27. [PMID: 18450749] (WB, Mouse)	WB	Mouse
Tancevski I, Wehinger A, Demetz E, Eller P, Duwensee K, Huber J, Hochegger K, Schgoer W, Fievet C, Stellaard F, Rudling M, Patsch JR, Ritsch A. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology;149(7):3708-12. 2008 Jul. [PMID: 18388200]
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Rode S, Rubic T, Lorenz RL et al. alpha-Tocopherol disturbs macrophage LXRalpha regulation of ABCA1/G1 and cholesterol handling. Biochem Biophys Res Commun 2008 May 9 [PMID: 18328805] (WB, Human)	WB	Human
Smoak K, Madenspacher J, Jeyaseelan S, Williams B, Dixon D, Poch KR, Nick JA, Worthen GS, Fessler MB. Effects of liver X receptor agonist treatment on pulmonary inflammation and host defense. J Immunol;180(5):3305-12. 2008 Mar 1. [PMID: 18292555]
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Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, Sato K, Hongob S, Nose K, Ota H, Kobayashi Y, Katagiri T, Shichiri M, Miyazaki A. Impact of salusin-alpha and -beta on human macrophage foam cell formation and coronary atherosclerosis. Circulation;117(5):638-48. 2008 Feb 5. [PMID: 18212280]
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem;283(18):12595-603. 2008 May 2. [PMID: 18211896]
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Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem;283(4):2147-55. 2008 Jan 25. [PMID: 18042552]
Wang N, Yvan-Charvet L, Lutjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J;22(4):1073-82. 2008 Apr. [PMID: 18039927]
Zhou X, He W, Huang Z, Gotto AM Jr, Hajjar DP, Han J. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 283(4):2129-38. 2008 Jan 25. [PMID: 18029360]
Yan D, Mayranpaa MI, Wong J, Perttila J, Lehto M, Jauhiainen M, Kovanen PT, Ehnholm C, Brown AJ, Olkkonen VM. OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem;283(1):332-40. 2008 Jan 4. [PMID: 17991739]
Out R, Jessup W, Le Goff W, Hoekstra M, Gelissen IC, Zhao Y, Kritharides L, Chimini G, Kuiper J, Chapman MJ, Huby T, Van Berkel TJ, Van Eck M. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res;102(1):113-20. 2008 Jan 4. [PMID: 17967783]
Hoe HS, Cooper MJ, Burns MP, Lewis PA, van der Brug M, Chakraborty G, Cartagena CM, Pak DT, Cookson MR, Rebeck GW. The metalloprotease inhibitor TIMP-3 regulates amyloid precursor protein and apolipoprotein E receptor proteolysis. J Neurosci;27(40):10895-905. 2007 Oct 3. [PMID: 17913923]
Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol;38(3):283-92. 2008 Mar. [PMID: 17884990] (IHC, Rat)	IHC	Rat
Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res;48(12):2647-63. 2007 Dec. [PMID: 17823458] (WB, Chicken)	WB	Chicken
Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation;116(12):1404-12. 2007 Sep 18. [PMID: 17724261]
Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res;48(11):2428-42. 2007 Nov. [PMID: 17656736]
During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007 Oct. [PMID: 17644776]
Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007 Oct. [PMID: 17609524]
Zelcer N, Khanlou N, Clare R et al. Attenuation of neuroinflammation and Alzheimer's disease pathology by liver x receptors. Proc Natl Acad Sci U S A 2007 Jun 19 [PMID: 17563384] (Mouse)		Mouse
Sporstol M, Mousavi SA, Eskild W et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol;8:5. 2007 Jan 22. [PMID: 17241464]
Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006 [PMID: 16807312] (WB, Porcine)	WB	Porcine
Trogan, E et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS 103:3781-3786. 2006 [PMID: 16537455] (IHC, Mouse)	IHC	Mouse
Dong J, Liu J, Lou B et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res;47(6):1307-14. 2006 Jun. [PMID: 16508036]
Gerbod-Giannone, M-C et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS 103:3112-3117. 2006 [PMID: 16492740]
Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH, Phillips MC. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res;47(4):832-43. 2006 Apr. [PMID: 16418537]
Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol;26(3):563-9. 2006 Mar. [PMID: 16410462]
Takata Y, Chu V, Collins AR et al. Transcriptional repression of ATP-binding cassette transporter A1 gene in macrophages: a novel atherosclerotic effect of angiotensin II. Circ Res 2005 Oct 28 [PMID: 16224068] (WB, Mouse)	WB	Mouse
Berfield, AK et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am J Physiol Renal Physiol 290:138-147. 2006 [PMID: 16077083]
Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol;25(8):1711-7. 2005 Aug. [PMID: 15961705] (WB, Human, Rat)	WB	Human, Rat
Glaros, EN et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J Biol Chem 280(26): 24515-24523. 2005 [PMID: 15890646] (WB, Human)	WB	Human
Ragozin S, Niemeier A, Laatsch A et al. Knockdown of hepatic ABCA1 by RNA interference decreases plasma HDL cholesterol levels and influences postprandial lipemia in mice. Arterioscler Thromb Vasc Biol 2005 Jul [PMID: 15845910]
Makowski L, Brittingham KC, Reynolds JM et al. The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem 2005 Apr 1 [PMID: 15684432] (EMSA, Mouse)	EMSA	Mouse
Li A et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J Clin Invest 114: 1564-1576. 2004 [PMID: 15578089]
Koldamova, R et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry 280(6): 4079-4088. 2005 [PMID: 15557325] (WB, Mouse)	WB	Mouse
Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol;25(1):128-34. 2005 Jan. [PMID: 15499044]
O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation;110(18):2881-8. 2004 Nov 2. [PMID: 15492319]
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell;15(12):5399-407. 2004 Dec. [PMID: 15469992]
Zeng, L et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J Biol Chem 279: 48801-48807. 2004 [PMID: 15358760] (WB, Human)	WB	Human
Denis, M et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry 279(40):41529-41536. 2004 [PMID: 15280376] (IP, Human)	IP	Human
Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. Exp Ther. 311(1):420-5. 2004 Oct. [PMID: 15210833]
Brown III, J et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry 279(33): 34674-34681. 2004 [PMID: 15148325] (WB, Human)	WB	Human
Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol;31(2):227-33. 2004 Aug. [PMID: 15039140]
Sahoo, D et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research 45: 1122-1131. 2004 [PMID: 14993246] (WB, Rat)	WB	Rat
Field, F et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research 45:905-913. 2004 [PMID: 14993242] (WB, Human)	WB	Human
Krimbou L, Denis M, Haidar B et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research 45:839-848. 2004 [PMID: 14754908] (IP, Human)	IP	Human
Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J;18(3):574-6. 2004 Mar. [PMID: 14734645]
Haidar, B et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J Biol Chem 279: 9963-9969. 2004 [PMID: 14701824] (IP, Human)	IP	Human
Gaus, K et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS 100(26): 15554-15559. 2003 [PMID: 14673117] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Denis, M et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry 279(9):7384-7394. 2004 [PMID: 14660648] (IP, Human)	IP	Human
Su, Y et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy 0:1-8. 2003 [PMID: 14529830] (WB, Mouse)	WB	Mouse
Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol;285(6):F1092-9. 2003 Dec. [PMID: 12952856]
Bortnick, A. Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878. 2003 [PMID: 12909583] (WB, Human)	WB	Human
Wagner, B. Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, pp 5780-5789. 2003Aug. [PMID: 12897148] (WB, Mouse)	WB	Mouse
Witting, S et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry 278(41):40121-40127. 2003 [PMID: 12890677] (WB, Mouse)	WB	Mouse
Khovidhunkit, W et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research 44: 1728-1736. 2003 [PMID: 12777468] (WB, Mouse)	WB	Mouse
Le Lay, S et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res 44(8): p 1499-1507. 2003 [PMID: 12754274] (WB, Mouse)	WB	Mouse
Sun, Y. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694. 2003 [PMID: 12754201] (WB, Mouse)	WB	Mouse
Terasaka, N et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters 536: 6-11. 2003 [PMID: 12586329] (IHC-P, Mouse)	IHC-P	Mouse
Basso, F et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research 44:296-302. 2003 [PMID: 12576511] (WB, Mouse)	WB	Mouse
Koldamova, R et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry 278(15):13244-13256. 2003 [PMID: 12547833] (WB, IHC, ICC/IF, Rat)	WB, IHC, ICC/IF	Rat
Escher, G et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry 278(13):11015-11019. 2003 [PMID: 12531903] (WB, Human, Hamster)	WB	Human, Hamster
Wang, N et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation111(1): 99-107. 2003 [PMID: 12511593] (WB, IP, Human)	WB, IP	Human
Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003 Jan [PMID: 12507911] (WB, Mouse)	WB	Mouse
Haidar, B et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research 43(12):2087-2094. 2002 [PMID: 12454270] (IP, Human)	IP	Human
Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol;22(11):1877-83. 2002 Nov 1. [PMID: 12426219]
Fukumoto, H et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry 277(50):48508-48513. 2002 [PMID: 12384498] (WB, Mouse, Human)	WB	Mouse, Human
Feng, B et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry 277(45):43271-73280. 2002 [PMID: 12215451] (WB, Mouse)	WB	Mouse
Murthy, S et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J Lipid Research 43: 1054-1064. 2002 [PMID: 12091489] (WB, Human)	WB	Human
Burgess, JW et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press 277(35): 31318-31326. 2002 [PMID: 12050168] (WB, Mouse, Human)	WB	Mouse, Human
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Reviews for ABCA1 Antibody (NB400-105) (6) 4.36

Average Rating: 4.3

(Based on 6 reviews)

We have 6 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB400-105:

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reviewed by:
Jahangir Iqbal

Mouse

11/16/2015

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Summary

Application	Western Blot
Sample Tested	mouse liver
Species	Mouse
Lot	AF-2

Blocking

Blocking Details

Membranes were blocked in 5% BSA in TBST for 1 h at room temperature

Primary Anitbody

Dilution Ratio

Membranes were immunoblotted with anti-ABCA1 antibody 1:2000 dilution in 2.5% BSA in TBST overnight at 4 oC

Secondary Antibody

Secondary Description	Membranes were incubated with secondary antibody conjugated to stabilized peroxidase for 1 hour at room temperature
Secondary Manufacturer Cat#	Thermo Scientific # 32460
Secondary Concentration	dilution 1:15,000

Details

Detection Notes

Bands were visualized used chemiluminescence according to the manufacturer’s instructions

Comments

Samples were not boiled and 2X BME was added.

reviewed by:
Anonymous

Mouse

12/23/2014

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Summary

Application	Western Blot
Sample Tested	See PMID 23519696
Species	Mouse

Blocking

Blocking Details

See PMID 23519696

Details

Detection Notes

See PMID 23519696

Comments

Independent review from antibodyresource.comPublished in PMID 23519696

reviewed by:
Angeliki Chroni

Human

11/03/2014

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Summary

Application	Immunofluorescence
Sample Tested	SK-N-SH human neuroblastoma cells
Species	Human

reviewed by:
Anonymous

Rat

10/15/2012

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Summary

Application	Western Blot
Sample Tested	Cardiomyocytes from newborn rats
Species	Rat

Blocking

Blocking Details

PBS Milk 5%, 1 hour, room temperature

Primary Anitbody

Dilution Ratio

1000 = dilution, 1 hour, room temperature, PBS without tween and 2% Milk

Secondary Antibody

Secondary Description	goat anti rabbit IgG HRP
Secondary Manufacturer Cat#	Amersham NA934V
Secondary Concentration	3000 diluted

Details

Detection Notes

ECL super plus Amersham RPN2132

reviewed by:
Cynthia Hong

Mouse

09/27/2011

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Summary

Application	Western Blot
Sample Tested	Mouse
Species	Mouse

reviewed by:
Anonymous

Other

06/15/2009

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Summary

Application	Western Blot
Sample Tested	Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
Species	Other

Blocking

Blocking Details

Blocking Buffer: 5% Milk w/ TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution Ratio

Primary Ab Dilution Ratio: 1/1000, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary Description

Secondary Ab: anti-Rabbit IgG HRP, Secondary Ab Dilution Ratio: 1/1000

Details

Detection Notes

Exposure Time: 1 hour, Positive Control: HC/RA, Specific Bands: 200 kDa

Product General Protocols

View specific protocols for ABCA1 Antibody (NB400-105):

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WB Video Protocol

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FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
- For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
- Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
- Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
- Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
- The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
- ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
Does this product contain any BSA?
- No, this antibody does not have any BSA in its formulation.
I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
- The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
- The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
- To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
Can i use DTT instead of Beta- mercaptoethanol?
- Either DTT or 2-mercaptoethanol is routinely used in SDS-PAGE sample buffer (reducing gel electrophoresis). Except that 2-mercaptoethanol is a stronger reducing agent and is mostly frequently used for that purpose.
Have you tested abca1 antibody(catalog # NB400-105) on hepatic Abca1 expression?
- We do not currently have any testing data where we have used NB400-105 with hepatic samples; all of our testing data for NB400-105 can be seen on the product page for this antibody. Should your customer be interested, we do show murine liver data with the following product: NB100-2068.

Show All 12 FAQs.

Control Lysate(s)

Raw 264.7 Whole Cell - T0901317 treated Assay Kit

Secondary Antibodies

Isotype Controls

Other Available Formats

Alexa Fluor 350	NB400-105AF350
Alexa Fluor 405	NB400-105AF405
Alexa Fluor 488	NB400-105AF488
Alexa Fluor 532	NB400-105AF532
Alexa Fluor 594	NB400-105AF594
Alexa Fluor 647	NB400-105AF647
Alexa Fluor 700	NB400-105AF700
Alexa Fluor 750	NB400-105AF750
Allophycocyanin/Cy7	NB400-105APCCY7
Biotin	NB400-105B
DyLight 350	NB400-105UV
DyLight 405	NB400-105V
DyLight 488	NB400-105G
DyLight 550	NB400-105R
DyLight 594	NB400-105DL594
DyLight 650	NB400-105C
DyLight 680	NB400-105FR
DyLight 755	NB400-105IR
FITC	NB400-105F
HRP	NB400-105H
Janelia Fluor 549	NB400-105JF549
Janelia Fluor 646	NB400-105JF646
PE	NB400-105PE
PE/Atto594	NB400-105PEATT594
PE/Cy5.5	NB400-105PECY55
PE/Cy7	NB400-105PECY7

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Bioinformatics Tool for ABCA1 Antibody (NB400-105)

Discover related pathways, diseases and genes to ABCA1 Antibody (NB400-105). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.

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Blogs on ABCA1. Showing 1-10 of 13 blog posts - Show all blog posts.

Check out the latest blog posts on ABCA1.

Phagocytes in Multiple Sclerosis: Myelin uptake leads to oxysterol-induced activation of liver X receptors, LXRs
By Jamshed Arslan Pharm.D., PhD.Myelin is a cholesterol-rich layer around nerves. Damage to this protective layer and the consequent slowing down of nerve impulses are hallmarks of multiple sclerosis (MS) and other... Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr... Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp... Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito... Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues... Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g.... Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP... Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee... Read full blog post.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa... Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)]
HAF008

Rabbit IgG Isotype Control
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Recent Reviews

4.3

5	3
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3	1
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	Jahangir Iqbal 11/16/2015
Application:	WB
Species:	Mouse

read this review

	Anonymous 12/23/2014
Application:	WB
Species:	Mouse

read this review

	Angeliki Chroni 11/03/2014
Application:	IF
Species:	Human

read this review

Bioinformatics

Gene Symbol	ABCA1
Entrez	19(Human) 11303(Mouse) 313210(Rat)
Uniprot	O95477(Human)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

ABCA1 Antibody

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Publications for ABCA1 Antibody (NB400-105)(351)

Reviews for ABCA1 Antibody (NB400-105) (6) 4.36

Summary

Blocking

Primary Anitbody

Secondary Antibody

Details

Comments

Summary

Blocking

Details

Comments

Summary

Summary

Blocking

Primary Anitbody

Secondary Antibody

Details

Summary

Summary

Blocking

Primary Anitbody

Secondary Antibody

Details

Product General Protocols

Video Protocols

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Other Available Formats

Additional ABCA1 Products

Bioinformatics Tool for ABCA1 Antibody (NB400-105)

Diseases for ABCA1 Antibody (NB400-105)

Pathways for ABCA1 Antibody (NB400-105)

PTMs for ABCA1 Antibody (NB400-105)

Research Areas for ABCA1 Antibody (NB400-105)

Blogs on ABCA1. Showing 1-10 of 13 blog posts - Show all blog posts.

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