ABCA1 Antibody - BSA Free

Images

 
Orthogonal Strategies: Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). ...read more
Genetic Strategies: HSKMCs were transfected with scrambled siRNA (scr siRNA) or ABCA1 siRNA. The cells were lysed and ABCA1 (A) protein levels were quantified by immunoblotting. Image collected and cropped by ...read more
Biological Strategies: Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total ...read more
Biological Strategies: HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then ...read more
Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.
Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. Representative image of ovarian ...read more
ABCA1 in human primary fibroblasts in culture. 10 ug of total protein. 7.5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4C. Secondary 1:5000 HRP 1h RT. Arrow shows around 250 kDa. WB ...read more
ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Biological Strategies: Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.
Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high cholesterol diet were analyzed by western ...read more
miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using NB400-105) and ABCG1 proteins in peritoneal macrophages from WT and KI+/+ mice. Representative images are shown. ...read more
miR-33b regulates ABCA1 and SREBP-1. Analysis for ABCA1, SREBP-1, CPT1a, and AMPK alpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. Representative images are shown. TF2B and beta-actin were used as ...read more
Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.
Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation.(A) Plasma concentrations of cholesterol were determined (N = 9/13 per group) after 5 weeks dietary ...read more
Gas6 treatment enhances expression of LXRalpha and LXRbeta and their target genes in BMDM.Mouse BMDM were stimulated with 400 ng/ml Gas6, 1 uM T0901317, 10 ng/ml interferon (IFN)-gamma, 10 ng/ml IL-4, or 100 ng/ml LPS ...read more
Western Blotting for ABCA1, as detailed in Supplementary Protocol #5 (Additional file 1). Caco-2 cells cultivated on filter inserts were treated for 48 h with bexarotene (RXR agonist, 5 uM), T0901317 (LXR agonist, 10 ...read more
Anti-ApoA-1 IgGs upregulate ABCA1. ABCA1 was increased after 16 and 24 h of anti-ApoA-1 IgG exposure to HMDM at the mRNA level as revealed by RT-PCR (a) as well as at the protein level after 24 h anti-apoA-1 IgG ...read more
ApoA-I increases glucose uptake in HSKMCs in an ABCA1- and SR-B1-dependent manner. HSKMCs were transfected with scrambled siRNA (scr siRNA), ABCA1 siRNA or SR-B1 siRNA. The cells were lysed and ABCA1 (A) and SR-B1 (B) ...read more
Functional miR-33 responsive elements in the 3'UTR of ATP8B1 and ABCB11. G). Relative protein levels in HuH7 cells transduced with empty or miR-33 adenovirus. Some cells were incubated for 16 h in the presence of ...read more
LXRs control expression of genes involved in cholesterol homeostasis and fatty acid synthesis in MPECs. (D) Western blot analysis was performed on WT (+/+) or Lxralphabeta-/- MPECs using Srebp1c, Abca1 and beta-Actin ...read more
Suppressive effects of Ucn1 on foam cell formation and related protein expression in human monocyte-derived macrophages.Human monocytes were incubated for 7 days with RPMI-1640 supplemented with 10% human serum and the ...read more
Alternatively activated macrophages (M2) exhibit increased clearance of OxLDL in vitro C). Increased ABCA1 and ABCG1 expression in M2-derived foam cells. Shown is a representative Western blot for the presence of ABCA1, ...read more
Association between expression ofABCA1and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. (A) Representative image of ovarian ...read more
Impairment of mitophagy upon Antimycin A and Oligomycin exposure.a Cells were treated with Antimycin A (1 μM) and Oligomycin (1 μM) for 1, 3, and 6 and mitochondrion/cytosol fractionation was performed to assay ...read more
GPR50 competes with FKBP12 for the binding to T beta RI. a (Left) HEK293T cells were transfected with Flag-T beta RI alone or cotransfected with HA-T beta RII (with TGF beta ; 2 ng/mL; 1 h), myc-FKBP12 and either ...read more
Involvement of CBP in BNIP3 expression regulated by HIF-1 alpha and FOXO3 under hypoxia. (A) UCB-hMSCs were incubated with hypoxia condition for 24 h. Co-immunoprecipitation of HIF-1 alpha and FOXO3 with IgG and CBP ...read more
DNA methylation and expression level of the PHD3 gene in HCT116 and DLD-1 CRC cells. A. HCT116 and DLD-1 cells were cultured under normoxic or hypoxic (1% O2) conditions for 48 hrs. Cells were then used for DNA ...read more
The role of HIF-1 in purinosome formation.a, quantifying the number of purinosome-containing cells in normoxia or hypoxia (24 h) in purine-rich medium and normoxia in purine-depleted medium (purine -ve), cells in ...read more
FIH exits the nucleus via a Leptomycin B-sensitive exportin1-dependent pathway. (A) Immunofluorescence staining of FIH (green) and HIF1 alpha (red) in MCF7 cells after the indicated hypoxia (0.5% O2) and re-oxygenation ...read more
Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium ...read more
Elevated Spy1 levels prevent contact inhibition. (A) Representative views of focus formation assays in NIH3T3 cells transfected with Myc-Spy1-WT, Myc-Spy1-TST, positive control Ras-V12 or empty vector PCS3 control. n = ...read more
SOD2 mRNA and protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, and highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Inhibition of KDM expression in vivo.(A) Ears from C57Bl/6 mice were injected intradermally with 250 ng of IL-1 alpha , IL-17A, IL-22, OSM and TNF alpha (M5) or with Phosphate Buffered Saline (PBS). At 24 h ...read more
MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA mimic ...read more
Intraperitoneally Administered Compound A Effectively Suppresses Triple-Negative Breast and Blood Cancer Xenograft Tumor Growth. Immunocompromised mice were subcutaneously injected with cancerous cell lines and tumors ...read more
Mechanical tensile strain of 30% induces an elongation of paranodal junction and alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR ...read more
Gomisin N (GN) represses the expression of JMJD2B, PPAR gamma 2, and PPAR gamma 2 steatosis target genes in HepG2 cells. HepG2 cells were treated with GN (100 μM) for 12 h. (A) JMJD2B expression was assessed by ...read more
TCAB1 knockdown inhibits tumor growth in vivo. a. shTCAB1-C and shScra cells were injected subcutaneously into the left and right neck of the nude mice respectively. Measured the tumors size from the 11th day using ...read more
MiR-375-3p negatively regulates Derlin-1 and blocks EMT in BFTC909 cells. (A) Western blot revealed the restoration of Derlin-1, MMP-2, Snail, and ZEB1 after co-transfection of miR-375-3p mimics and CMV-Derlin-1 ...read more
JMJD2B expression increases in hepatic steatotic cell and animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, and ...read more
TLR4 expression in pVW-MSCs cultured with or without LPS (10 μg/ml) for 1 and 4 h and after additional 24 h of recovery after LPS removal (4hR). a: flow cytometry analysis were performed in not fixed and not ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IP, WB, B/N, ChIP, Dual ISH-IHC, Func, GS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

ABCA1 Antibody - BSA Free Summary

Immunogen
Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Localization
Astrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize reported in scientific literature (PMID 30821416)
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 19515742)
  • Dual RNAscope ISH-IHC
  • ELISA reported in scientific literature (PMID 18541924)
  • Flow Cytometry 1:400. Use reported in scientific literature (PMID 21501868)
  • Gel Super Shift Assays reported in scientific literature (PMID 15684432)
  • Gel Supershift Assay
  • Immunoblotting reported in scientific literature (PMID 27599291)
  • Immunocytochemistry/ Immunofluorescence 1:100. Use reported in scientific literature (PMID 21501868)
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 21846716; 21106520)
  • Knockdown Validated reported in scientific literature (PMID 31666189)
  • Knockout Validated reported in scientific literature (PMID 32273567)
  • PCR reported in scientific literature (PMID 27406916)
  • Simple Western 1:50
  • Western Blot 1:500
Application Notes
Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Charge.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raw 264.7 Whole Cell - T0901317 treated Assay Kit
THP-1 TO901317 Treated / Untreated Cell Lysate
Control Peptide
ABCA1 Protein (NB400-105PEP)
Reviewed Applications
Read 7 Reviews rated 4.3
using
NB400-105 in the following applications:

Publications
Read Publications using
NB400-105 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCA1 Antibody - BSA Free

  • ABC1
  • ABC-1
  • ABC1ATP-binding cassette transporter A1
  • ABCA1
  • ATP-binding cassette 1
  • ATP-binding cassette transporter 1
  • ATP-binding cassette, sub-family A (ABC1), member 1
  • CERP
  • Cholesterol efflux regulatory protein
  • EC 2.7.7.8
  • EC 2.8.1.8
  • EC 3.6.3
  • EC 3.6.3.41
  • FLJ14958
  • HDLDT1
  • membrane-bound
  • MGC164864
  • MGC165011
  • TGD
  • TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA1 Antibody (NB400-105)(396)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Canine, Chicken, Chinese Hamster, Hamster, Mustelid, Porcine, Rabbit.

We have publications tested in 15 applications: B/N, Chemotaxis, ELISA, EMSA, FLOW, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB.


Filter By Application
B/N
(2)
Chemotaxis
(1)
ELISA
(1)
EMSA
(1)
FLOW
(3)
IB
(1)
ICC/IF
(24)
IF/IHC
(19)
IHC
(2)
IHC-Fr
(7)
IHC-P
(9)
IP
(9)
KD
(1)
KO
(1)
WB
(211)
All Applications
Filter By Species
Human
(99)
Mouse
(142)
Rat
(23)
Canine
(2)
Chicken
(1)
Chinese Hamster
(1)
Hamster
(6)
Mustelid
(1)
Porcine
(3)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 396. Show All 396 Publications.
Publications using NB400-105 Applications Species
Mao M, Deng Y, Wang L et al. Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE(-/-) mice PeerJ 2023-09-04 [PMID: 37692113]
Wiener JP, Desire S, Garliyev V et al. Down-Regulation of ABCA7 in Human Microglia, Astrocyte and THP-1 Cell Lines by Cholesterol Depletion, IL-1? and TNF?, or PMA Cells 2023-08-25 [PMID: 37681876]
Resetar M, Tietcheu Galani BR, Tsamo AT et al. Flindissone, a Limonoid Isolated from Trichilia prieuriana, Is an LXR Agonist Journal of Natural Products 2023-08-25 [PMID: 37526502] (WB, B/N) WB, B/N
Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (IHC-Fr, ICC/IF) IHC-Fr, ICC/IF
Molina-Gonzalez I, Holloway RK, Jiwaji Z et al. Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration Nature communications 2023-06-08 [PMID: 37291151] (ICC/IF, Mouse) ICC/IF Mouse
Miyagawa S, Horie T, Nishino T et al. Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitis Life science alliance 2023-08-01 [PMID: 37263777] (WB, Mouse, Human) WB Mouse, Human
Cao D, Khan Z, Li X et al. Macrophage angiotensin-converting enzyme (ACE) reduces atherosclerosis by increasing PPAR? and fundamentally changing lipid metabolism Cardiovascular research 2023-05-24 [PMID: 37225143] (WB, Mouse) WB Mouse
Dragoljevic D, Lee MKS, Pernes G et al. Administration of an LXR agonist promotes atherosclerotic lesion remodelling in murine inflammatory arthritis Clinical & translational immunology 2023-04-18 [PMID: 37091327] (IHC, Mouse)

Details:
Mouse lesions in arthritic mice
IHC Mouse
Cashikar A, Rios D, Timm D et al. Regulation of astrocyte lipid metabolism and ApoE secretion by the microglial oxysterol, 25-hydroxycholesterol Journal of lipid research 2023-02-25 [PMID: 36849076] (Mouse) Mouse
Li Z, He M, Chen G et al. Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice bioRxiv : the preprint server for biology 2023-02-07 [PMID: 36798262] (WB, Mouse) WB Mouse
Show All 396 Publications.

Reviews for ABCA1 Antibody (NB400-105) (7) 4.37

Average Rating: 4.3
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB400-105:
Filter by Applications
WB
(6)
IF
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ABCA1 NB400-105
Enlarge
4
reviewed by:
Verified Customer
WB Human 08/15/2021
View

Summary

ApplicationWestern Blot
Sample TestedHuman primary fibroblast
SpeciesHuman
LotAM-5

Comments

Comments10 ug of total protein. 7,5% TGX gel. Blocking 5% milk in PBST 1h RT. 1:1000 primary ab in BSA 3% PBST O/N at 4ºC. Secondary 1:5000 HRP 1h RT.
Western Blot ABCA1 NB400-105
Enlarge
4
reviewed by:
Jahangir Iqbal
WB Mouse 11/16/2015
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotAF-2
  3
reviewed by:
Verified Customer
WB Mouse 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23519696
SpeciesMouse
  5
reviewed by:
Angeliki Chroni
IF Human 11/03/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSK-N-SH human neuroblastoma cells
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Rat 10/15/2012
View

Summary

ApplicationWestern Blot
Sample TestedCardiomyocytes from newborn rats
SpeciesRat
  5
reviewed by:
Cynthia Hong
WB Mouse 09/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Other 06/15/2009
View

Summary

ApplicationWestern Blot
Sample TestedRat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
SpeciesOther

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

  1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
    • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
  2. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
    • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
  3. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
    • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
  4. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
    • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
  5. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
    • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
  6. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
    • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
  7. Does this product contain any BSA?
    • No, this antibody does not have any BSA in its formulation.
  8. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
    • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
  9. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
    • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
  10. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
    • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
  11. Show All 12 FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ABCA1 Products

Research Areas for ABCA1 Antibody (NB400-105)

Find related products by research area.

Blogs on ABCA1. Showing 1-10 of 14 blog posts - Show all blog posts.

HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins
Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho...  Read full blog post.

Phagocytes in Multiple Sclerosis: Myelin uptake leads to oxysterol-induced activation of liver X receptors, LXRs
By Jamshed Arslan Pharm.D., PhD.Myelin is a cholesterol-rich layer around nerves. Damage to this protective layer and the consequent slowing down of nerve impulses are hallmarks of multiple sclerosis (MS) and other ...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito...  Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ...  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
3
4
3
3
1
2
0
1
0

Verified Customer
08/15/2021
Application: WB
Species: Human

Jahangir Iqbal
11/16/2015
Application: WB
Species: Mouse

 
Verified Customer
12/23/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ABCA1
Entrez
Uniprot