ABCA1 Antibody

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # ...read more
Biological Strategies: Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior ...read more
Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then ...read more
Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA-1 FITC conjugated antibody of human adipose tissue macrophage subsets by flow cytometry. Image from verified customer review.
Western Blot: ABCA1 Antibody [NB400-105] - Prostates of LXR mutant mice accumulate cholesterol esters through inappropriate LXR target genes regulation. Total protein lysates of WT and LXR null mice under normal or high ...read more
Western Blot: ABCA1 Antibody [NB400-105] - miR-33b regulates ABCA1 and SREBP-1. Western blotting analysis for ABCA1, SREBP-1, CPT1a, and AMPKalpha protein levels in the livers of WT, KI+/-, and KI+/+ mice. ...read more
Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC ...read more
Flow Cytometry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Biological Strategies: Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced ...read more
Western Blot: ABCA1 Antibody [NB400-105] - miR-33b reduces cellular cholesterol efflux and serum HDL-C levels. Western blotting for ABCA1 (using ABCA1 Antibody NB400-105) and ABCG1 proteins in peritoneal macrophages ...read more
Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbSpecies Glossary
Applications WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ABCA1 Antibody Summary

Immunogen
Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Localization
Astrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry 1:400
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • Dual RNAscope ISH-IHC
  • Gel Supershift Assay
  • Knockdown Validated
  • PCR
Application Notes
This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). Use in immunoblotting reported in scientific literature (PMID: 27599291). Use in blocking/ neutralizing reported in scientific literature (PMID: 30821416). Use in PCR reported in scientific literature (PMID: 27406916). Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation (PMID 19515742) and ELISA (PMID 18541924) were also reported in scientific literature. Knockdown validation (PMID: 31666189). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Charge.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raw 264.7 Whole Cell - T0901317 treated Assay Kit
Control Peptide
Reviewed Applications
Read 6 Reviews rated 4.3
using
NB400-105 in the following applications:

Publications
Read Publications using
NB400-105 in the following applications:

  • B/N
    1 publication
  • 1 publication
  • 1 publication
  • 1 publication
  • 1 publication
  • 2 publications
  • IB
    1 publication
  • 20 publications
  • IHC
    14 publications
  • 1 publication
  • 3 publications
  • 6 publications
  • IP
    8 publications
  • KD
    1 publication
  • WB
    172 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24612239). Rabbit reactivity reported in scientific literature (PMID: 26444796). Chinese Hamster reactivity reported in scientific literature (PMID: 27902765). Primate reactivity reported in scientific literature (PMID: 25440061). Equine reactivity reported in scientific literature (PMID: 26711702).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCA1 Antibody

  • ABC1
  • ABC-1
  • ABC1ATP-binding cassette transporter A1
  • ABCA1
  • ATP-binding cassette 1
  • ATP-binding cassette transporter 1
  • ATP-binding cassette, sub-family A (ABC1), member 1
  • CERP
  • Cholesterol efflux regulatory protein
  • EC 2.7.7.8
  • EC 2.8.1.8
  • EC 3.6.3
  • EC 3.6.3.41
  • FLJ14958
  • HDLDT1
  • membrane-bound
  • MGC164864
  • MGC165011
  • TGD
  • TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCA1 Antibody (NB400-105)(325)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Canine, Chicken, Chinese Hamster, Hamster, Mustelid, Porcine, Rabbit.

We have publications tested in 15 applications: B/N, ChIP, ELISA, EMSA, FLOW, Flow, IB, ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P, IP, KD, WB.


Filter By Application
B/N
(1)
ChIP
(1)
ELISA
(1)
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(1)
FLOW
(1)
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(2)
IB
(1)
ICC/IF
(20)
IHC
(14)
IHC-FR
(1)
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(3)
IHC-P
(6)
IP
(8)
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(1)
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(172)
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Human
(85)
Mouse
(107)
Rat
(19)
Canine
(2)
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(6)
Mustelid
(1)
Porcine
(3)
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(2)
All Species
Showing Publications 1 - 10 of 325. Show All 325 Publications.
Publications using NB400-105 Applications Species
Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. Mar 27 2020 [PMID: 32292343] (WB, Rat) WB Rat
Bogie Jfj, Grajchen E, Wouters E et Al. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain J. Exp. Med. May 4 2020 [PMID: 32097464] (Flow, Mouse) Flow Mouse
Matsuda J, Takahashi A, Takabatake Y et al. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells Autophagy Jan 16 2020 [PMID: 31944172]
Chen Y, Guo KM, Nagy T, Guo TL Metabolomics study of the prefrontal cortex in a rat model of attention deficit hyperactivity disorder reveals the association between cholesterol metabolism disorder and hyperactive behavior Biochem. Biophys. Res. Commun. Dec 18 2019 [PMID: 31864712] (WB, Rat) WB Rat
Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. Nov 14 2019 [PMID: 31771811] (WB, Mouse) WB Mouse
Du Y, Li X, Su C et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice Br. J. Pharmacol. Nov 25 2019 [PMID: 31769014]
Pagano S, Magenta A, D'Agostino M et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation J Clin Med Nov 21 2019 [PMID: 31766415] (WB, Human) WB Human
Kaymak I, Maier CR, Schmitz W et al. Mevalonate pathway provides ubiquinone to maintain pyrimidine synthesis and survival in p53-deficient cancer cells exposed to metabolic stress Cancer Res. Nov 19 2019 [PMID: 31744820] (WB, Human, Mouse) WB Human, Mouse
Xiao L, Luo G, Guo X et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages Biochim Biophys Acta Mol Cell Biol Lipids 2019 Oct 27 [PMID: 31666189] (ICC/IF, WB, KD, Human, Mouse) ICC/IF, WB, KD Human, Mouse
Smith TKT, Kahiel Z, LeBlond ND et al. Characterization of Redox-Responsive LXR-Activating Nanoparticle Formulations in Primary Mouse Macrophages Molecules Oct 18 2019 [PMID: 31635211] (WB, Mouse) WB Mouse
Show All 325 Publications.

Reviews for ABCA1 Antibody (NB400-105) (6) 4.36

Average Rating: 4.3
(Based on 6 reviews)
We have 6 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB400-105:
Filter by Applications
WB
(5)
IF
(1)
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Human
(1)
Mouse
(3)
Rat
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot ABCA1 NB400-105
Enlarge
reviewed by:
Jahangir Iqbal
WB Mouse 11/16/2015
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotAF-2

Blocking

Blocking DetailsMembranes were blocked in 5% BSA in TBST for 1 h at room temperature

Primary Anitbody

Dilution RatioMembranes were immunoblotted with anti-ABCA1 antibody 1:2000 dilution in 2.5% BSA in TBST overnight at 4 oC

Secondary Antibody

Secondary DescriptionMembranes were incubated with secondary antibody conjugated to stabilized peroxidase for 1 hour at room temperature
Secondary Manufacturer Cat#Thermo Scientific # 32460
Secondary Concentrationdilution 1:15,000

Details

Detection NotesBands were visualized used chemiluminescence according to the manufacturer’s instructions

Comments

CommentsSamples were not boiled and 2X BME was added.
 
reviewed by:
Anonymous
WB Mouse 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23519696
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23519696

Details

Detection NotesSee PMID 23519696

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23519696
 
reviewed by:
Angeliki Chroni
IF Human 11/03/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSK-N-SH human neuroblastoma cells
SpeciesHuman
 
reviewed by:
Anonymous
WB Rat 10/15/2012
View

Summary

ApplicationWestern Blot
Sample TestedCardiomyocytes from newborn rats
SpeciesRat

Blocking

Blocking DetailsPBS Milk 5%, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1000 = dilution, 1 hour, room temperature, PBS without tween and 2% Milk

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgG HRP
Secondary Manufacturer Cat#Amersham NA934V
Secondary Concentration3000 diluted

Details

Detection NotesECL super plus Amersham RPN2132
 
reviewed by:
Cynthia Hong
WB Mouse 09/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
 
reviewed by:
Anonymous
WB Other 06/15/2009
View

Summary

ApplicationWestern Blot
Sample TestedRat astrocyte, Human Astrocyte, and Raw Mouse Neuron, Sample Amount: 25ug to 50ug
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: 5% Milk w/ TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/1000, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-Rabbit IgG HRP, Secondary Ab Dilution Ratio: 1/1000

Details

Detection NotesExposure Time: 1 hour, Positive Control: HC/RA, Specific Bands: 200 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ABCA1 Antibody (NB400-105). (Showing 1 - 10 of 12 FAQs).

  1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
    • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.
  2. Do you have a protocol for Flow cytometry analysis with this ABCA1 antibody (NB400-105)?
    • Please see this link to our protocol for Flow cytometry. This is a general protocol that our lab uses as a guideline.
  3. We purchased this antibody a few weeks ago, and we by mistake stored it at -20 degrees Celsius. Is there possible the antibody still works?
    • Most likely the antibody should still work, but we cannot guarantee it. The biggest thing with antibody storage in most cases, especially for unconjugated antibodies, will be to avoid freeze/thaw cycles as much as possible. So even with ones that we recommend to freeze long term we still recommend aliquoting so they can go through as few of those as possible. I would say since it will probably only undergo one when you move it back to 4 degrees Celsius you should be fine, but again I can't say with 100% certainty. If you have a problem with detection using this product, please let us know so that we can figure out other possibilities to help you in that situation.
  4. I am interested in looking at this marker on monocytes by flow cytometry. I noticed that a directly conjugated 488 ab is available, and that your flow cytometry protocol calls for methanol fixation of the cells. Is the fixation required for antibody binding, or may it be used on cells that have not been methanol fixed? I would like to retain other markers.
    • Methanol is not required for antibody binding, but it is a fixation method that we found to not interfere with the antibody-antigen binding. You may use unfixed cells, but you may not be able to achieve staining. If you fix your cells with a formaldehyde-based fixative (we recommend antigen retrieval for IHC). However, as an inter-membrane protein, you will need to permeabilize your cells in order for the antibody to access the protein.
  5. To do Western blot for the determination of ABCA1 protein level, do I need to order NB400-105 or NB400-105B? Which one do you recommend if we also order the secondary antibody catalog # NB730-H?
    • The difference between NB400-105 or NB400-105B is biotin conjugation. It is up to you whether you would like to order the primary antibody that is conjugated or not. With the biotin-conjugated antibody (catalog # NB400-105B), you won't need a secondary, just a (strept)avidin-labelled detection reagent, such as streptavidin-HRP. With the unconjugated primary (catalog # NB400-105), you will need to use a secondary antibody. Some people say that when you use the secondary, there is an amplification of signal, because more than one secondary can bind to each primary. However, with the directly conjugated antibody you have fewer reagents and fewer wash steps.
  6. We purchased NB400-105, Lot# Z5, but the datasheet (approx. 450kDa) was differ from your website (approx. 220kDa) for size of WB. Which molecular weight is correct?
    • ABCA1 is highly glycosylated and has a predicted molecular weight of approximately 250 kDa without post-translational modification. The apparent molecular weight will change based on treatment and sample. All of the images on our website and datasheet are correct.
  7. Does this product contain any BSA?
    • No, this antibody does not have any BSA in its formulation.
  8. I bought ABCA1 antibody (NB400-105) from your company recently and used it in Flow cytometry. I want to know the binding site of ABCA1 and the antibody; is it within the cell or outside? Also, do I need to change the cytomembrane's permeabilization before my test?
    • The immuogen region of NB400-105 is within residues 1100-1300 of human ABCA1 and as a result it does not cover the extracellular domains, as a consequence, permeabilization is required for this antibody. As a general guideline, we usually recommend using 0.5-1.0% Triton detergent in the buffers in order to allow full permeabilization of our antibody.
  9. Is post-nuclear lysate is necessary? Can't I lysate my cells with RIPAbuffer (#R0278 sigma)?
    • The post-nuclear lysate you mentioned is posted on our website to demonstrate how we do the experiments, and how it was done by our collaborator (Ref Link #1). The procedure of getting the post-nuclear lysate was just for removing the nuclei before lysis and therefore the cleaner the lysates would be. You do not need to follow the method for the experiment that led to the result.
  10. Why shouldn't I boil the samples? is it OK to boil them if using RIPAbuffer?
    • To fractionate a membrane-bound protein, the lipid moieties associated with the protein frequently cause the smearing or streaky appearance of our WB. To result this difficulty, it is advised that we resuspend cell pellets in the SDS-sample buffer, left at room temp without boiling, spinning down the viscous cell debris, and then subject to PAGE and blotting. And this was the reason we suggested you not to boil your protein samples. Whether it will work for resuspending cell pellets in RIPA buffer, unfortunately we have no such data.
  11. Show All 12 FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-105AF350
Alexa Fluor 405 NB400-105AF405
Alexa Fluor 488 NB400-105AF488
Alexa Fluor 532 NB400-105AF532
Alexa Fluor 594 NB400-105AF594
Alexa Fluor 647 NB400-105AF647
Alexa Fluor 700 NB400-105AF700
Alexa Fluor 750 NB400-105AF750
Allophycocyanin/Cy7 NB400-105APCCY7
Biotin NB400-105B
DyLight 350 NB400-105UV
DyLight 405 NB400-105V
DyLight 488 NB400-105G
DyLight 550 NB400-105R
DyLight 594 NB400-105DL594
DyLight 650 NB400-105C
DyLight 680 NB400-105FR
DyLight 755 NB400-105IR
FITC NB400-105F
HRP NB400-105H
Janelia Fluor 549 NB400-105JF549
Janelia Fluor 646 NB400-105JF646
PE NB400-105PE
PE/Atto594 NB400-105PEATT594
PE/Cy5.5 NB400-105PECY55
PE/Cy7 NB400-105PECY7

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Blogs on ABCA1. Showing 1-10 of 13 blog posts - Show all blog posts.

Phagocytes in Multiple Sclerosis: Myelin uptake leads to oxysterol-induced activation of liver X receptors, LXRs
By Jamshed Arslan Pharm.D., PhD.Myelin is a cholesterol-rich layer around nerves. Damage to this protective layer and the consequent slowing down of nerve impulses are hallmarks of multiple sclerosis (MS) and other...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito...  Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee...  Read full blog post.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Recent Reviews

4.3
5
3
4
2
3
1
2
0
1
0

Jahangir Iqbal
11/16/2015
Application: WB
Species: Mouse

 
Anonymous
12/23/2014
Application: WB
Species: Mouse

 
Angeliki Chroni
11/03/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol ABCA1
Entrez
Uniprot
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