This ABCA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence (PMID: 21501868), Western blot and Immunohistochemistry on frozen and paraffin embedded sections. In Western blot a band representing ABCA1 is observed at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. ELISA has been reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB400-105F in the following applications:
Canine reactivity reported in scientific literature (PMID: 24612239)
Alternate Names for ABCA1 Antibody [FITC]
ABC1ATP-binding cassette transporter A1
ATP-binding cassette 1
ATP-binding cassette transporter 1
ATP-binding cassette, sub-family A (ABC1), member 1
Cholesterol efflux regulatory protein
TGDATP-binding cassette sub-family A member 1
ABCA1 (ATP-binding cassette transporter A1), ATP-binding cassette transporter family member, is a cAMP-dependent and sulfonylurea-sensitive anion transporter which is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux from macrophages and act as a key gatekeeper influencing intracellular cholesterol transport. ABCA1 is widely expressed with highest expression levels found in macrophages and is induced by bacterial LPS which regulates its expression through LXR (liver X receptor) -independent mechanism. ABCA1 interacts with MEGF10 and its expression is repressed by ZNF202. ABCA1 phosphorylation at Ser-2054 site regulates the phospholipid efflux and DHHC8 mediated palmitoylation of ABCA1 is essential for its membrane localization. ABCA1 expressed in brain neurons/glia mediates cholesterol transport to apoE which is the major CNS apolipoprotein, and it influences the motor function and synaptic morphology also. Defects in ABCA1 have been linked to HDLD1 (high density lipoprotein deficiency type 1; also known as analphalipoproteinemia, Tangier disease or TGD) and HDLD2 (high density lipoprotein deficiency type 2; also known as familial hypoalphalipoproteinemia or FHA).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Niemann Pick-C1 and cholesterol dynamics Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post.
ABCA1 (ATP-binding cassette transporter A1) The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr... Read full blog post.
ABCG8: Cholesterol's Fate The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito... Read full blog post.
ABCA1 ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues... Read full blog post.
LXR Alpha, ABCA1 and Cholesterol Homeostasis LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee... Read full blog post.
Exploring how ABCG8 Affects Heart Disease The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th... Read full blog post.