SR-BI Antibody

Images

 
Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP-conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1000 dilution, shows distinct band at 82 kDa.
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% ...read more
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on SK-MEL-28 cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control NBP2-24893PE (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified customer review. Image using the DyLight 550 form of ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on A549 cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SR-BI Antibody Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 2.5-5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5-5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in IHC-Frozen reported in scientific literature (PMID 26865459).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SR-BI Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB400-104 in the following applications:

Publications
Read Publications using
NB400-104 in the following applications:

  • 8 publications
  • 2 publications
  • 10 publications
  • IHC
    7 publications
  • 1 publication
  • 7 publications
  • IP
    1 publication
  • WB
    75 publications

Reactivity Notes

Reactivity with CHO cells reported in scientific literature (PMID: 12356718). Porcine reactivity reported in scientific literature (PMID: 26852751).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-104
Species: Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHa
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for SR-BI Antibody (NB400-104)(184)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Chinese Hamster, Golden Syrian Hamster, Hamster, Porcine, Primate, Rabbit.

We have publications tested in 8 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
FLOW
(8)
Flow
(2)
ICC/IF
(10)
IHC
(7)
IHC-Fr
(1)
IHC-P
(7)
IP
(1)
WB
(75)
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Human
(43)
Mouse
(46)
Rat
(13)
Chinese Hamster
(1)
Golden Syrian Hamster
(1)
Hamster
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 184. Show All 184 Publications.
Publications using NB400-104 Applications Species
Dolezelova E, SA ICI, Prasnicka A et al. High soluble endoglin levels regulate cholesterol homeostasis and bile acids turnover in the liver of transgenic mice Life Sci. Jul 9 2019 [PMID: 31299237] (WB, Mouse) WB Mouse
Zurkirchen L, Varum S, Giger S et al. Upregulation of Scavenger receptor B1 is required for steroidogenic and non-steroidogenic cholesterol metabolism in prostate cancer Cancer Res. May 7 2019 [PMID: 31064850] (WB, Human) WB Human
Huang, L;Chambliss, KL;Gao, X;Yuhanna, IS;Behling-Kelly, E;Bergaya, S;Ahmed, M;Michaely, P;Luby-Phelps, K;Darehshouri, A;Xu, L;Fisher, EA;Ge, WP;Mineo, C;Shaul, PW; SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature Apr 24 2019 [PMID: 31019307] (IHC-P, Mouse) IHC-P Mouse
Wu ZY, Li H, Li JR et al. Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I Eur. J. Pharmacol. Mar 19 2019 [PMID: 30902657] (WB, Human) WB Human
Tsuzuki S, Lee S, Kimoto Y et al. A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae Biomed. Res. Dec 12 2018 [PMID: 30531159] (IHC, Mouse) IHC Mouse
Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse) WB Mouse
Cartocci V, Tonini C, Di Pippo T et al. Prenatal exposure to valproate induces sex-, age-, and tissue-dependent alterations of cholesterol metabolism: Potential implications on autism. J. Cell. Physiol. Oct 20 2018 [PMID: 30341891] (WB, Rat) WB Rat
Cairns R, Fischer AW, Blanco-Munoz P et al. Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet PLoS ONE Aug 15 2018 [PMID: 30110341] (WB, Mouse) WB Mouse
Sun Y, Zhang D, Liu X et al. Endoplasmic Reticulum Stress Affects Lipid Metabolism in Atherosclerosis Via CHOP Activation and Over-Expression of miR-33 Cell. Physiol. Biochem. Aug 9 2018 [PMID: 30092598] (WB, Mouse) WB Mouse
Sticozzi C, Pecorelli A, Romani A et al. Tropospheric ozone affects SRB1 levels via oxidative post-translational modifications in lung cells. Free Radic. Biol. Med. Jul 27 2018 [PMID: 30059777] (WB, Human) WB Human
Show All 184 Publications.

Reviews for SR-BI Antibody (NB400-104) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB400-104:
Filter by Applications
ICC
(1)
Flow
(2)
WB
(1)
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Human
(2)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SR-BI NB400-104
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reviewed by:
Pierre Jean Beltran
ICC Human 04/05/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast
SpeciesHuman

Comments

CommentsSR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% bovine serum albumin. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).
Flow Cytometry SR-BI NB400-104
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reviewed by:
Ryan Russell
Flow Human 11/06/2012
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Summary

ApplicationFlow Cytometry
Sample TestedHepG2 liver cells
SpeciesHuman
LotP-1
CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1:5000, 5% milk in TBST 4 degrees overnight in

Details

Detection NotesPVDF membrane. Thermo Scientific Chemiluminescent Substrate, 5-min. negative control: eNOS; positive control: actin

Comments

CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.
Western Blot SR-BI NB400-104
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reviewed by:
Mitchell Knutson
WB Rat 10/23/2012
View

Summary

ApplicationWestern Blot
Sample TestedH4IIE total cell lysates and plasma membrane proteins
SpeciesRat
LotP-1

Blocking

Blocking Details5% Non fat dry milk in TBST, 1 h, RT

Primary Anitbody

Dilution Ratio1:2000 in blocking buffer, overnight incubation at 4 degrees

Secondary Antibody

Secondary DescriptionHRP-conjugated donkey anti-rabbit igG
Secondary Manufacturer Cat#GE healthcare NA9340V
Secondary Concentration1:2000-1:5000

Details

Detection NotesWest Pico (Pierce), visualized with FluoroChem E (Cell Biosciences)
 
reviewed by:
Anonymous
Flow Mouse 11/23/2011
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse
SpeciesMouse
CommentsThe signal is strong and clear

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 100x, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Details

Detection NotesDetection Visualization Method: LSM 700 Confocal Microscopy, Secondary Ab: Goat anti-rabbit IgG FITC

Comments

CommentsThe signal is strong and clear

Product General Protocols

View specific protocols for SR-BI Antibody (NB400-104): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104). (Showing 1 - 3 of 3 FAQs).

  1. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  2. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
  3. Do the cells have to be permeabilized before using this antibody for flow cytometry?
    • For flow cytometry, we do recommend fixation and permeabilization of the cell prior to incubation with this antibody. The product NB400-104 recognizes an epitope in the cytoplasmic domain of this membrane protein. Usually our lab will recommend using 0.1-0.5% Tween-20 for membrane permeabilization. Sometimes, you can also achieve ample permeabilization of the membrane using methanol fixation. Our lab's flow protocol can be found here: Flow Cytometry protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-104AF350
Alexa Fluor 405 NB400-104AF405
Alexa Fluor 488 NB400-104AF488
Alexa Fluor 532 NB400-104AF532
Alexa Fluor 594 NB400-104AF594
Alexa Fluor 647 NB400-104AF647
Alexa Fluor 700 NB400-104AF700
Alexa Fluor 750 NB400-104AF750
Allophycocyanin/Cy7 NB400-104APCCY7
Biotin NB400-104B
DyLight 350 NB400-104UV
DyLight 405 NB400-104V
DyLight 488 NB400-104G
DyLight 550 NB400-104R
DyLight 594 NB400-104DL594
DyLight 650 NB400-104C
DyLight 680 NB400-104FR
DyLight 755 NB400-104IR
FITC NB400-104F
HRP NB400-104H
Janelia Fluor 549 NB400-104JF549
Janelia Fluor 646 NB400-104JF646
PE NB400-104PE
PE/Atto594 NB400-104PEATT594
PE/Cy5.5 NB400-104PECY55
PE/Cy7 NB400-104PECY7

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Pierre Jean Beltran
04/05/2017
Application: ICC
Species: Human

Ryan Russell
11/06/2012
Application: Flow
Species: Human

Mitchell Knutson
10/23/2012
Application: WB
Species: Rat

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot