SR-BI Antibody

Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP-conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1000 dilution, shows distinct band at 82 kDa.

Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% BSA. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).

Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.

Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on SK-MEL-28 cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.

Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.

Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.

Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control NBP2-24893PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: SR-BI Antibody [NB400-104] - SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified customer review. Image using the DyLight 550 form of this antibody.

Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on A549 cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHa
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SR-BI Antibody Summary

• Immunogen: A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt Q61009]
• Localization: Cell Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCARB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 2.5-5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5-5 ug/ml
  • Immunoprecipitation 1:10-1:500
• Application Notes: In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in IHC-Frozen reported in scientific literature (PMID 26865459).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SR-BI Overexpression Lysate

Reactivity Notes

Reactivity with CHO cells reported in scientific literature (PMID: 12356718). Porcine reactivity reported in scientific literature (PMID: 26852751).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SR-BI Antibody

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-BI Antibody (NB400-104)(186)

Publications using NB400-104

• Huang J, Yin H, Yin P et al. SR-BI Interactome Analysis Reveals a Proviral Role for UGGT1 in Hepatitis C Virus Entry Front Microbiol Sep 6 2019 [PMID: 31551978] (WB, Human)
• Zhang J, Qu C, Li T et al. Phagocytosis mediated by scavenger receptor class BI promotes macrophage transition during skeletal muscle regeneration J. Biol. Chem. Aug 28 2019 [PMID: 31462534] (WB, KO, Mouse)
• Dolezelova E, SA ICI, Prasnicka A et al. High soluble endoglin levels regulate cholesterol homeostasis and bile acids turnover in the liver of transgenic mice Life Sci. Jul 9 2019 [PMID: 31299237] (WB, Mouse)
• Zurkirchen L, Varum S, Giger S et al. Upregulation of Scavenger receptor B1 is required for steroidogenic and non-steroidogenic cholesterol metabolism in prostate cancer Cancer Res. May 7 2019 [PMID: 31064850] (WB, Human)
• Huang, L;Chambliss, KL;Gao, X;Yuhanna, IS;Behling-Kelly, E;Bergaya, S;Ahmed, M;Michaely, P;Luby-Phelps, K;Darehshouri, A;Xu, L;Fisher, EA;Ge, WP;Mineo, C;Shaul, PW; SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature Apr 24 2019 [PMID: 31019307] (IHC-P, Mouse)
• Wu ZY, Li H, Li JR et al. Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I Eur. J. Pharmacol. Mar 19 2019 [PMID: 30902657] (WB, Human)
• Tsuzuki S, Lee S, Kimoto Y et al. A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae Biomed. Res. Dec 12 2018 [PMID: 30531159] (IHC, Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Cartocci V, Tonini C, Di Pippo T et al. Prenatal exposure to valproate induces sex-, age-, and tissue-dependent alterations of cholesterol metabolism: Potential implications on autism. J. Cell. Physiol. Oct 20 2018 [PMID: 30341891] (WB, Rat)
• Cairns R, Fischer AW, Blanco-Munoz P et al. Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet PLoS ONE Aug 15 2018 [PMID: 30110341] (WB, Mouse)
• Sun Y, Zhang D, Liu X et al. Endoplasmic Reticulum Stress Affects Lipid Metabolism in Atherosclerosis Via CHOP Activation and Over-Expression of miR-33 Cell. Physiol. Biochem. Aug 9 2018 [PMID: 30092598] (WB, Mouse)
• Sticozzi C, Pecorelli A, Romani A et al. Tropospheric ozone affects SRB1 levels via oxidative post-translational modifications in lung cells. Free Radic. Biol. Med. Jul 27 2018 [PMID: 30059777] (WB, Human)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (Human)
• Bruening J, Lasswitz L, Banse P et al. Hepatitis C virus enters liver cells using the CD81 receptor complex proteins calpain-5 and CBLB. PLoS Pathog. Jul 1 2018 [PMID: 30024968] (ICC/IF, Human)
• Zeng Z, Cao B, Guo X et al. Apolipoprotein B-100 peptide 210 antibody inhibits atherosclerosis by regulation of macrophages that phagocytize oxidized lipid. Am J Transl Res Jun 15 2018 [PMID: 30018722] (WB, Mouse)
• Tsuzuki S, Kimoto Y, Lee S et al. A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity Biomed. Res. Jun 15 2018 [PMID: 29899187] (WB, Mouse)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018 May 26 [PMID: 29802792] (Human)
• Adamson SX, Lin Z, Chen R et al. Experimental challenges regarding the in vitro investigation of the nanoparticle-biocorona in disease states Toxicol In Vitro Sep 1 2018 [PMID: 29738787] (Flow, Mouse)
• Gao F, Li G, Liu C et al. Autophagy regulates testosterone synthesis by facilitating cholesterol uptake in Leydig cells. J. Cell Biol. Apr 4 2018 [PMID: 29618492] (Mouse)
• Durham KK, Chathely KM, Trigatti BL. High density lipoprotein protects cardiomyocytes against necrosis induced by oxygen and glucose deprivation, through SR-B1, PI3K, and AKT1 and 2. Biochem. J. Mar 9 2018 [PMID: 29523748] (Mouse)
• Schobel A, Rosch K, Herker E. Functional innate immunity restricts Hepatitis C Virus infection in induced pluripotent stem cell-derived hepatocytes. Sci Rep. Mar 1 2018 [PMID: 29497123] (WB, Human)
• Lee SX, Heine M, Schlein C, Ramakrishnan R. FoxO transcription factors are required for hepatic HDL-cholesterol clearance. J. Clin. Invest. 2018 Feb 06 [PMID: 29408809] (WB, Mouse)
• Pewkliang Y, Rungin S, Lerdpanyangam K et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax Malar. J. 2018 Jan 25 [PMID: 29370800] (ICC/IF, Human)
• Zhu L, Shi J, Luu TN et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Molecular Metabolism 2017 [PMID: 29331506] (Mouse)
• Cartocci V, Catallo M, Tempestilli M et al. Altered Brain Cholesterol/Isoprenoid Metabolism in a Rat Model of Autism Spectrum Disorders Neuroscience 2018 Jan 06 [PMID: 29309878] (Rat)
• Milligan S, Martin GG, Landrock D et al. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice. Biochim Biophys Acta Mol Cell Biol Lipids 2018 Mar [PMID: 29307784] (Mouse)
• Fung KY, Wang C, Nyegaard S et al. SR-BI Mediated Transcytosis of HDL in Brain Microvascular Endothelial Cells Is Independent of Caveolin, Clathrin, and PDZK1 Front Physiol. 2017 Oct 30 [PMID: 29163190] (WB, Human)
• Xian X, Ding Y, Dieckmann M et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis Elife. 2017 Nov 16 [PMID: 29144234] (WB, Mouse)
• Muresan XM, Sticozzi C, Belmonte G et al. Modulation of cutaneous scavenger receptor B1 levels by exogenous stressors impairs in vitro wound closure Mech. Ageing Dev. 2017 Nov 01 [PMID: 29102450] (Human)
• Durham K, Chathely K, Mak KC et al. HDL protects against doxorubicin induced cardiotoxicity in a scavenger receptor class B type 1, PI3K, and AKT dependent manner.. Am. J. Physiol. Heart Circ. Physiol. 2017 Oct 06 [PMID: 28986362] (ICC/IF, Human)
• Rink JS, Yang S, Cen O et al. Rational Targeting of Cellular Cholesterol in Diffuse Large B-Cell Lymphoma (DLBCL) Enabled by Functional Lipoprotein Nanoparticles: A Therapeutic Strategy Dependent on Cell of Origin. Mol Pharm. 2017 Sep 21 [PMID: 28933554] (Flow)
  
  Details:
  This citation used the Alexa Fluor 647 form of this antibody.
• Qian Y, Qiao S, Dai Y et al. Molecular-Targeted Immunotherapeutic Strategy for Melanoma via Dual-Targeting Nanoparticles Delivering Small Interfering RNA to Tumor-Associated Macrophages ACS Nano 2017 Sep 26 [PMID: 28858473] (WB, Mouse)
• Lai CJ, Cheng HC, Lin CY et al. Activation of liver X receptor suppresses angiogenesis via induction of ApoD FASEB J. 2017 Aug 21 [PMID: 28842423] (WB, Human)
• Russelli G, Pizzillo P, Iannolo G et al. HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation PLoS ONE 2017 Jul 27 [PMID: 28750044] (ICC/IF, Human)
• Castella B, Kopecka J, Sciancalepore P et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vg9Vdelta2 T cell activation by dendritic cells. Nat Commun. 2017 Jun 05 [PMID: 28580927] (WB, Human)
• Fletcher RB, Das D, Gadye L et al. Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution. Cell Stem Cell Apr 28 2017 [PMID: 28506465]
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)
• Geng S, Chen K, Yuan R et al. The persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis. Nat Commun. Nov 8 2016 [PMID: 27824038] (Mouse)
• Cedo L, Garcia-Leon A, Baila-Rueda L et al. ApoA-I mimetic administration, but not increased apoA-I-containing HDL, inhibits tumour growth in a mouse model of inherited breast cancer. Sci Rep. Nov 3 2016 [PMID: 27808249] (WB, Mouse)
• Song G, Wu X, Zhang P et al. High-density lipoprotein inhibits ox-LDL-induced adipokine secretion by upregulating SR-BI expression and suppressing ER Stress pathway. Sci Rep. Jul 29 2016 [PMID: 27468698] (WB)
• Li J, Wang J, Li M et al. Up-regulated expression of scavenger receptor class B type 1 (SR-B1) is associated with malignant behaviors and poor prognosis of breast cancer. Pathol. Res. Pract. 2016 Mar 31 [PMID: 27067809] (IHC-P, WB, Human)
  
  Details:
  SR-BI antibody was used for WB and IHC-P on human samples.
• Julovi SM, Xue A, Thanh LE TN et al. Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo PLoS ONE Mar 23 2016 [PMID: 27002321] (WB, IHC-P, Human)
• Cheng JJ, Li JR, Huang MH et al. CD36 is a co-receptor for hepatitis C virus E1 protein attachment Sci Rep Feb 22 2016 [PMID: 26898231] (WB, Human)
• Ganesan LP, Mates JM, Cheplowitz AM et al. Scavenger receptor B1, the HDL receptor, is expressed abundantly in liver sinusoidal endothelial cells. Sci Rep. 2016 Feb 11 [PMID: 26865459] (IHC-Fr, Mouse)
• Hong L, Xu X, Huang J et al. Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency Sci Rep Feb 8 2016 [PMID: 26852751] (IHC-P, Porcine)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)
• Mysore R, Zhou Y, Sadevirta S et al. MicroRNA-192* impairs adipocyte triglyceride storage. Biochim. Biophys. Acta. 2015 Dec 30 [PMID: 26747651] (WB, Human)
• Cameron SJ, Morrell CN, Bao C et al. A Novel Anti-Inflammatory Effect for High Density Lipoprotein. PLoS ONE. 2015 Dec 18 [PMID: 26680360] (WB, Human)
• Nadeem AE, Thomas P, Ulf ML et al. Cell culture-derived HCV cannot infect synovial fibroblasts. Sci Rep. 2015 Dec 08 [PMID: 26643193] (WB, Human)
• Bartels ED, Christoffersen C, Lindholm MW, Nielsen LB. Altered Metabolism of LDL in the Arterial Wall Precedes Atherosclerosis Regression. Circ. Res. 2015 Sep 10 [PMID: 26358193] (IHC, Mouse)
• Calattini S, Fusil F, Mancip J et al. Functional and Biochemical Characterization of Hepatitis C Virus (HCV) Particles Produced in a Humanized Liver Mouse Model. J Biol Chem 2015 Sep 18 [PMID: 26224633]
• Yan X, Shen T, Jiang X et al. Coenzyme Q10 consumption promotes ABCG1-mediated macrophage cholesterol efflux: a randomized, double-blind, placebo-controlled, cross-over study in healthy volunteers. Mol Nutr Food Res 2015 Sep [PMID: 26081100]
• Frentzen A, Kusuma A, Guerlevik E et al. Cell entry, efficient RNA replication, and production of infectious hepatitis C virus progeny in mouse liver-derived cells. Hepatology 2013 Jul 19 [PMID: 23873628] (FLOW, Mouse)
• Bura KS, Lord C, Marshall S et al. Intestinal SR-BI does not impact cholesterol absorption or transintestinal cholesterol efflux in mice. J Lipid Res 2013 Jun [PMID: 23564696]
• Kim S, Ishida H, Yamane D et al. Contrasting Roles of Mitogen-activated Protein Kinases in Cellular Entry and Replication of Hepatitis C Virus: MKNK1 Facilitates Cell Entry. J Virol 2013 Jan 30 [PMID: 23365451] (WB, Human)
• Nam H, Wang CY, Zhang L et al. ZIP14 and DMT1 in the liver, pancreas, and heart are differentially regulated by iron deficiency and overload: implications for tissue iron uptake in iron-related. Haematologica 2013 Jan 24 [PMID: 23349308] (WB, Mouse, Rat)
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation Biochem Pharmacol 2013 Jan 9 [PMID: 23313547] (WB, Human)
• Escola-Gil JC, Julve J, Llaverias G et al. Resveratrol administration or SIRT1 overexpression does not increase LXR signaling and macrophage-to-feces reverse cholesterol transport in vivo Transl Res 2012 Nov 10 [PMID: 23146569] (WB, Mouse)
• Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013 Jan [PMID: 23117655] (WB, Mouse)
• Shen C, Jiang J, Huang C, Zhu W. Gypenoside LVI attenuates foam cell formation by promoting cholesterol export and inhibiting inflammation response Oncotarget Oct 5 2018
• Pierre J, Rommel M, Ileana C. A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection. Cell Systems 2016 Oct 26 [PMID: 27641956] (ICC/IF, Human)
• Scharinger B, Messner B, Turkcan A et al. Leoligin, the major lignan from Edelweiss, inhibits 3-hydroxy-3-methyl-glutaryl-CoA reductase and reduces cholesterol levels in ApoE-/- mice J Mol Cell Cardiol 2016 Oct [PMID: 27497529] (WB)
  
  Details:
  This reference used the HRP version of NB400-104.
• Ulitzky Laura, Lafer Manuel M, KuKuruga Mark A et al. A New Signaling Pathway for HCV Inhibition by Estrogen: GPR30 Activation Leads to Cleavage of Occludin by MMP-9. PloS One 2016 [PMID: 26731262] (WB, Human)
• Sane A, Seidman E, Spahis S et al. New Insights in Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis J. Cell. Biochem. 2015 Mar 30 [PMID: 25826777] (WB, Human)
• Wang Y, Meng C, Wei Q et al. Expression and regulation of scavenger receptor class B type 1 in the rat ovary and uterus during the estrous cycle. Acta Histochem. 2015 Mar 26 [PMID: 25817199] (WB, IHC-P, Rat)
• Ng S, Schwartz RE, March S et al. Human iPSC-Derived Hepatocyte-like Cells Support Plasmodium Liver-Stage Infection In Vitro Stem Cell Reports. 2015 Feb 04 [PMID: 25660406] (ICC/IF, WB, Human)
  
  Details:
  SR-BI antibody used for ICC-IF (Figure 1F and 1G) and WB (Figure 1E) on iPSC-derived hepatocyte-like cells / iHLCs at days between 10-20 of differentiation. ICC-IF protocol involved - fixation in chilled methanol for 10 min at 4C, blocking for ON at 4C with 2% BSA-PBS, primary antibody used at 1:100 dilution with ON 4C incubation, detection with Alexa Fluor donkey anti-rabbit secondary antibody and Hoechst 33258 counterstaining.
• Ali Kamilah, Abo-Ali Ehab M, Kabir M D et al. A Western-fed diet increases plasma HDL and LDL-cholesterol levels in apoD-/- mice. PLoS One. 2014 Dec 30 [PMID: 25548917] (WB, Mouse)
• Ye R, Holland Wl, Gordillo R et al. Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes B-cell regeneration. Elife. 2014 Oct 23 [PMID: 25339419] (IHC, Mouse)
• Bankwitz D, Vieyres G, Hueging K et al. Role of the hypervariable region 1 (HVR1) for the interplay of hepatitis C virus with entry factors and lipoproteins. J Virol. 2014 Aug 20 [PMID: 25142595] (WB, Human)
• Matsuda M, Suzuki R, Kataoka C et al. Alternative endocytosis pathway for productive entry of hepatitis C virus. J Gen. Virol. 2014 Aug 05 [PMID: 25096815] (WB, Human)
• Xu Y, Liu Q, Xu Y et al. Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT. J Lipid Res. 2014 Jun 7 [PMID: 24908654]
• Poulaert M, Gunata Z, During A et al. Hesperidin increases intestinal betta, betta-carotene 15-15'mono-oxygenase 1 (BCMO1) activity in Mongolian gerbils (< i> Meriones unguiculatus</i>) fed with betta-carotene- …. Food Chemistry 3/13/2014 [PMID: 24767085] (WB)
• Valacchi G, Belmonte G, Miracco C et al. Effect of combined mulberry leaf and fruit extract on liver and skin cholesterol transporters in high fat diet-induced obese mice. Nutr Res Pract 2/1/2014 [PMID: 24611101] (IHC-P, Mouse)
• Bertolin K, Gossen J, Schoonjans K. The Orphan Nuclear Receptor Nr5a2 is Essential for Luteinization in the Female Mouse Ovary. Endocrinology 2014 Feb 20 [PMID: 24552399] (IHC-P, Mouse)
• Sticozzi C, Belmonte G, Cervellati F et al. Resveratrol protects SR-B1 levels in keratinocytes exposed to cigarette smoke. Free Radic. Biol. Med. 2014 Jan 12 [PMID: 24423486] (WB, Mouse)
• Ying R, Yuan Y, Qin YF. The combination of L-4F and simvastatin stimulate cholesterol efflux and related proteins expressions to reduce atherosclerotic lesions in apoE knockout mice. Lipids Health Dis 2013 Dec 8 [PMID: 24314261] (WB, Mouse)
• Rohrl C, Eigner K, Winter K et al. Endoplasmic reticulum stress impairs cholesterol efflux and synthesis in hepatic cells. J. Lipid Res. 2014 Jan 1 [PMID: 24179149] (WB, Human)
• Danilo C, Gutierrez-Pajares JL, Mainieri MA et al. Scavenger receptor class B type I regulates cellular cholesterol metabolism and cell signaling associated with breast cancer development. Breast Cancer Res. 2013 Sep 24 [PMID: 24060386] (WB, IHC, Human, Mouse)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013 Feb 19 [PMID: 23334894] (WB, Mouse)
• (WB, Mouse)
• Dube E, Ethier-Chiasson M, Lafond J. Modulation of cholesterol transport by insulin-treated gestational diabetes mellitus in human full-term placenta. Biol Reprod 2013 Jan 17 [PMID: 23221398]
• Koga T, Ichinose K, Mizui M et al. Calcium/calmodulin-dependent protein kinase IV suppresses IL-2 production and regulatory T cell activity in lupus. J Immunol 2012 Oct 1 [PMID: 22942433]
• Wang CY, Jenkitkasemwong S, Duarte S et al. ZIP8 Is an Iron and Zinc Transporter Whose Cell-surface Expression Is Upregulated by Cellular Iron Loading J Biol Chem 2012 Aug 16 [PMID: 22898811] (WB, Rat)
• Spillmann F, Miteva K, Warstat K, Perisic S. High-density Lipoproteins Induce the Migration Capacity of Mesenchymal Stromal Cells. J Stem Cell Res Ther 2/22/2014 (FLOW, Human)
• Jarajapu YP, Cai J, Yan Y et al. Protection of Blood Retinal Barrier and Systemic Vasculature by Insulin-Like Growth Factor Binding Protein-3 plos One 2012 [PMID: 22792172] (IHC, ICC/IF, Rat)
• Dao Thi VL, Granier C, Zeisel MB et al. Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps J Biol Chem 2012 Sep 7 [PMID: 22767607] (WB, Human)
• Kotake-Nara E, Nagao A. Effects of mixed micellar lipids on carotenoid uptake by human intestinal Caco-2 cells Biosci Biotechnol Biochem 2012 [PMID: 22738952] (WB, Human)
• Cox JV, Naher N, Abdelrahman YM, BellRJ. Host HDL biogenesis machinery is recruited to the inclusion of Chlamydia trachomatis-infected cells and regulates chlamydial growth Cell Microbiol 2012 Jun 4 [PMID: 22672264]
• Lundasen T, Andersson EM, Snaith M, Lindmark H, Lundberg J, Ostlund-Lindqvist AM, Angelin B, Rudling M. Inhibition of intestinal bile Acid transporter slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice. PLoS One;7(5):e37787. 2012 [PMID: 22662222] (WB, Mouse)
• Grefhorst A, Oosterveer MH, Brufau G et al. Pharmacological LXR activation reduces presence of SR-B1 in liver membranes contributing to LXR-mediated induction of HDL-cholesterol Atherosclerosis, Mar 3 2012 [PMID: 22481067] (WB, Mouse)
• Broderius M, Mostad E, Prohaska JR. Suppressed hepcidin expression correlates with hypotransferrinemia in copper-deficient rat pups but not dams Genes Nutr 2012 Mar 29 [PMID: 22457245] (WB, Rat)
• Sticozzi C, Belmonte G, Pecorelli A et al. Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation PLoS One 2012 [PMID: 22442701]
• BriF, Thieblemont Q, Muzotte E, Sulpice T. High-fat and fructose intake induces insulin resistance, dyslipidemia, and liver steatosis and alters in vivo macrophage-to-feces reverse cholesterol transport in hamsters J Nutr 2012 Apr [PMID: 22357742]
• Naples M, Baker C, Lino M, Iqbal J, Hussain MM, Adeli K. Ezetimibe ameliorates intestinal chylomicron overproduction and improves glucose tolerance in a diet-induced hamster model of insulin resistance. Am J Physiol Gastrointest Liver Physiol;302(9):G1043-52. 2012 May. [PMID: 22345552] (WB, Golden Syrian Hamster)
• Molina-Jimenez F, Benedicto I, Dao Thi VL et al. Matrigel-embedded 3D culture of Huh-7 cells as a hepatocyte-like polarized system to study hepatitis C virus cycle. Virology. 2012 Jan 24. [PMID: 22280897]
• Storey SM, McIntosh AL, Huang H et al. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2012 Jan 12. [PMID: 22241858]
• Kuang YL, Paulson KE, Lichtenstein AH, Lamon-Fava S. Regulation of the expression of key genes involved in HDL metabolism by unsaturated fatty acids Br J Nutr 2012 Oct 28 [PMID: 22221450]
• Ji A, Wroblewski JM, Cai L et al. Nascent HDL formation in hepatocytes and role of ABCA1, ABCG1 and SR-BI. Journal of Lipid Research. 2011 Dec 20. [PMID: 22190590]
• Kammerer I, Ringseis R, Biemann R et al. 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages. Lipids in Health and Disease 3010(1):222. 2011 Nov. [PMID: 22129452]
• Reboul E, Soayfane Z, Goncalves A et al. Respective contributions of intestinal Niemann-Pick C1-like 1 and scavenger receptor class B type I to cholesterol and tocopherol uptake: in vivo v. in vitro studies. Br J Nutr 2012 May [PMID: 21929836]
• Dong P, Xie T, Zhou X et al. Induction of macrophage scavenger receptor type BI expression by tamoxifen and 4-hydroxytamoxifen. Atherosclerosis. Oct. 2011 [PMID: 21820658] (WB, Mouse)
• Corcoran MP, Lichtenstein AH, Meydani M et al. The effect of 17{beta}-estradiol on cholesterol content in human macrophages is influenced by the lipoprotein milieu. J Mol Endocrinol. 2011 Jun 6. [PMID: 21646386] (WB, Human)
• Ji A, Meyer JM, Cai L et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011 Apr 8. [PMID: 21481393]
• Vaziri ND, Gollapudi P, Han S, FarahmG, Yuan J, Rahimi A, Moradi H. Nephrotic syndrome causes upregulation of HDL endocytic receptor and PDZK-1-dependent downregulation of HDL docking receptor. Nephrol Dial Transplant;26(10):3118-23. 2011 Oct. [PMID: 21459782] (WB, IHC, Rat)
• Taketa Y, Yoshida M, Inoue K et al. Differential stimulation pathways of progesterone secretion from newly formed corpora lutea in rats treated with ethylene glycol monomethyl ether, sulpiride, or atrazine. Toxicol Sci;121(2):267-278. 2011 Jun. [PMID: 21427058] (IHC, Rat)
• Koller E, Vincent TM, Chappell A et al. Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytes. Nucleic Acids Res. 2011 Feb 23. [PMID: 21345934]
• Win SJ, Ward VK, Dunbar PR et al. Cross-presentation of epitopes on virus-like particles via the MHC I receptor recycling pathway. Immunol Cell Biol 2011 Aug [PMID: 21221122]
• Law RH, Lukoyanova N, Voskoboinik I et al. The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 2010 Nov 18 [PMID: 21037563] (WB)
• Srivastava Roshni, Yu Shaohua, Parks Brian W et al. Autoimmune-mediated reduction of high-density lipoprotein-cholesterol and paraoxonase 1 activity in systemic lupus erythematosus-prone gld mice. Arthritis and Rheumatism 2011 [PMID: 20882670] (WB, Mouse)
• Jenkitkasemwong S, Broderius M, Nam H et al. Anemic copper-deficient rats, but not mice, display low hepcidin expression and high ferroportin levels. J Nutr;140(4):723-30. 2010 Apr. [PMID: 20164366] (WB, Mouse, Rat)
• Tancevski I, Demetz E, Eller P et al. The liver-selective thyromimetic T-0681 influences reverse cholesterol transport and atherosclerosis development in mice. PLoS One 2010 [PMID: 20090943] (Mouse)
• Schafer G, Guler R, Murray G et al. The role of scavenger receptor B1 in infection with Mycobacterium tuberculosis in a murine model. PLoS One 2009 [PMID: 20041149] (WB)
• Hoppmann S, Steinbach J, Pietzsch J. Scavenger receptors are associated with cellular interactions of S100A12 in vitro and in vivo. Int J Biochem Cell Biol 2010 May [PMID: 20025991] (WB)
• Gantman A, Fuhrman B, Aviram M, Hayek T. High glucose stimulates macrophage SR-BI expression and induces a switch in its activity from cholesterol efflux to cholesterol influx. Biochem Biophys Res Commun 2010 Jan 1 [PMID: 19941833] (Mouse)
• Haid S, Windisch MP, Bartenschlager R et al. Mouse-specific residues of claudin-1 limit hepatitis C virus genotype 2a infection in a human hepatocyte cell line. J Virol;84(2):964-75. 2010 Jan. [PMID: 19889758]
• Mailhot G, Ravid Z, Barchi S et al. CFTR knockdown stimulates lipid synthesis and transport in intestinal Caco-2/15 cells. Am J Physiol Gastrointest Liver Physiol;297(6):G1239-49. 2009 Dec. [PMID: 19808659]
• Tao W, Xu C, Ding Q et al. A single point mutation in E2 enhances hepatitis C virus infectivity and alters lipoprotein association of viral particles. Virology 2009 Dec 5 [PMID: 19793603] (FLOW)
• Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res;105(5):500-510. 2009 [PMID: 19644050]
• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression cholesterol efflux from human macrophages. Am J Physiol Endocrinol Metab;297(2):E474-482. 2009 [PMID: 19625677]
• Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009 [PMID: 19590047]
• Benedicto I, Molina-Jimenez F, Bartosch B et al. The Tight Junction-Associated Protein Occludin Is Required for a Postbinding Step in Hepatitis C Virus Entry Infection. J Virol;83(16):8012-8020. 2009 [PMID: 19515778]
• Arakawa R, Tsujita M, Iwamoto N et al. Pharmacological inhibition of ABCA1 degradation increases HDL biogenesis and exhibits antiatherogenesis. J Lipid Res;50(11):2299-305. 2009 Nov. [PMID: 19458386] (WB, Rabbit)
• Bujold K, Rhainds D, Jossart C et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res;83(3):457-464. 2009 [PMID: 19377069]
• Kim HJ, Moradi H, Yuan J et al. Renal mass reduction results in accumulation of lipids dysregulation of lipid regulatory proteins in the remnant kidney. Am J Physiol Renal Physiol 90761.92008. [PMID: 19357177]
• Karasinska JM, Rinninger F, Lutjohann D et al. Specific Loss of Brain ABCA1 Increases Brain Cholesterol Uptake Influences Neuronal Structure Function. J Neurosci;29(11):3579-3589. 2009 [PMID: 19295162]
• Atshaves BP, McIntosh AL, Martin GG et al. Overexpression of sterol carrier protein-2 differentially alters hepatic cholesterol accumulation in cholesterol-fed mice. J Lipid Res;50(7):1429-1447. 2009 [PMID: 19289417]
• Neumann S, Coudreuse DY, van der Westhuyzen DR et al. Mammalian Wnt3a is released on lipoprotein particles. Traffic 2009 Mar [PMID: 19207483]
• Beaslas O, Cueille C, Delers F et al. Sensing of dietary lipids by enterocytes: a new role for SR-BI/CLA-1. PLoS One 2009 [PMID: 19169357] (IP, Human)
• Burke KT, Colvin PL, Myatt L et al. Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster. J Lipid Res;50(6):1146-1155. 2009 [PMID: 19122238] (WB, Hamster)
• Siebrasse JP, Veith R, Dobay A et al. Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin. Proc Natl Acad Sci U S A 2008 Dec 23 [PMID: 19074261]
• Iwao Y, Nakajou K, Nagai R et al. CD36 is one of important receptors promoting renal tubular injury by advanced oxidation protein products. Am J Physiol Renal Physiol;295(6):F1871-80. 2008 Dec. [PMID: 18971214]
• Shimizu E, Kawahara K, Kajizono M et al. IL-4-induced selective clearance of oligomeric beta-amyloid peptide(1-42) by rat primary type 2 microglia. J Immunol;181(9):6503-13. 2008 Nov 1. [PMID: 18941241]
• Pou J, Rebollo A, Roglans N et al. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood);233(12):1572-82. 2008 Dec. [PMID: 18849545]
• Cherian-Shaw M, Puttabyatappa M, Greason E et al. Expression of Scavenger Receptor-BI Low-Density Lipoprotein Receptor Differential Use of Lipoproteins to Support Early Steroidogenesis in Luteinizing Macaque Granulosa Cells. Endocrinology;150(2):957-65. 2009 Feb. [PMID: 18832102] (WB, Primate)
• Martin GG, Atshaves BP, McIntosh AL et al. Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity. J Nutr;138(10):1859-65. 2008 Oct. [PMID: 18806093]
• Bulat N, Waeber G, Widmann C. LDLs stimulate p38 MAPKs and wound healing through SR-BI independently of Ras and PI3 kinase. J Lipid Res;50(1):81-9. 2009 Jan. [PMID: 18757346]
• Karpac J, Czyzewska K, Kern A et al. Failure of adrenal corticosterone production in POMC-deficient mice results from lack of integrated effects of POMC peptides on multiple factors. Am J Physiol Endocrinol Metab;295(2):E446-55. 2008 Aug. [PMID: 18559987]
• van der Velde AE, Vrins CLJ, van den Oever K et al. Regulation of direct transintestinal cholesterol excretion in mice. Am J Physiol Gastrointest Liver Physiol;295(1):G203-G208. 2008 Jul. [PMID: 18511744]
• Frank PG, Pavlides S, Cheung MWC et al. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol;295(1):C242-8. 2008 Jul. [PMID: 18508910]
• Li L, Thompson PA, Kitchens RL. Infection induces a positive acute phase apolipoprotein E response from a negative acute phase gene: role of hepatic LDL receptors. J Lipid Res;49(8):1782-93. 2008 Aug. [PMID: 18497424]
• During A, Doraiswamy S, Harrison EH. Xanthophylls are preferentially taken up compared with beta-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res;49(8):1715-24. 2008 Aug. [PMID: 18424859]
• Komori H, Arai H, Kashima T et al. Coexpression of CLA-1 and human PDZK1 in murine liver modulates HDL cholesterol metabolism. Arterioscler Thromb Vasc Biol 2008 Jul [PMID: 18403724]
• Tancevski I, Wehinger A, Demetz E et al. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology;149(7):3708-12. 2008 Jul. [PMID: 18388200]
• Drover VA, Nguyen DV, Bastie CC et al. CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice. J Biol Chem;283(19):13108-15. 2008 May 9. [PMID: 18332148]
• Ashraf, M Z, N S Kar et al. Specific oxidized phospholipids inhibit scavenger receptor bi-mediated selective uptake of cholesteryl esters. J Biol Chem;283(16):10408-14. 2008 Apr 18. [PMID: 18285332]
• Montoudis, A, Seidman, E, Boudreau, F et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008 May. [PMID: 18235139]
• Barth H, Schnober EK, Neumann-Haefelin C et al. Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells. J Virol;82(7):3466-79. 2008 Apr. [PMID: 18216094]
• Tietge UJF, Nijstad N, Havinga R et al. Secretory phospholipase A2 increases SR-BI-mediated selective uptake from HDL but not biliary cholesterol secretion. J Lipid Res;49(3):563-71. 2008 Mar. [PMID: 18037706]
• Eacker SM, Agrawal N, Qian K et al. Hormonal regulation of testicular steroid and cholesterol homeostasis. Mol Endocrinol;22(3):623-35. 2008 Mar. [PMID: 18032697]
• Zeisel MB, Koutsoudakis G, Schnober EK et al. Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81. Hepatology 2007 Dec [PMID: 18000990] (FLOW)
• Valiyaveettil, M, N Kar et al. Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI. Blood. 111(4):1962-71. 2008 Feb 15. [PMID: 17993610] (FLOW, WB, Human, Mouse)
• Yvan-Charvet L, Pagler TA, Wang N et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res;49(1):107-14. 2008 Jan. [PMID: 17960026]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007 Oct. [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR et al. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007 Oct. [PMID: 17609524]
• Thomas-Moya E, Gianotti M, Proenza AM et al. Paraoxonase 1 response to a high-fat diet: gender differences in the factors involved. Mol Med 2007 Mar-Apr [PMID: 17592556] (WB, Rat)
• Qu, Shen et al. Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice. J Lipid Res;48(7):1476-87. 2007 Jul. [PMID: 17438339]
• Timmers, C et al. E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop. Mol Cell Biol;27(1):65-78. 2007 Jan. [PMID: 17167174]
• Harder CJ, Meng A, Rippstein P et al. SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells. J Biol Chem 2007 Jan 12 [PMID: 17105723]
• Wadsack, C et al. Intrauterine growth restriction is associated with alterations in placental lipoprotein receptors and maternal lipoprotein composition. Am J Physiol Endocrinol Metab;292(2):E476-84. 2007 Feb. [PMID: 17003234]
• Zhong, J et al. Persistent hepatitis C virus infection in vitro: coevolution of virus and host. J Virol;80(22):11082-93. 2006 Nov. [PMID: 16956932] (FLOW, Human)
• Azhar, S et al. LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells. J Lipid Res;47(11):2538-50. 2006 Nov. [PMID: 16929031] (ICC/IF, Rat)
• Akpovi, CD et al. The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J Lipid Res;47(10):2233-47. 2006 Oct. [PMID: 16861621]
• Sane, AT et al. Localization and role of NPC1L1 in cholesterol absorption in human intestine. J Lipid Res;47(10):2112-20. 2006 Oct. [PMID: 16829661]
• Thomas-Moya, E et al. The age-related paraoxonase 1 response is altered by long-term caloric restriction in male and female rats. J Lipid Res;47(9):2042-8. 2006 Sep. [PMID: 16816326]
• Zhang, C et al. NO-1886 upregulates ATP binding cassette transporter A1 and inhibits diet-induced atherosclerosis in Chinese Bama minipigs. J Lipid Res;47(9):2055-63. 2006 Sep. [PMID: 16807312]
• Heo TH, Lee SM, Bartosch B et al. Hepatitis C virus E2 links soluble human CD81 and SR-B1 protein. Virus Res 2006 Oct [PMID: 16725222]
• Ikura Y, Ohsawa M, Suekane T et al. Localization of oxidized phosphatidylcholine in nonalcoholic fatty liver disease: impact on disease progression. Hepatology 2006 Mar [PMID: 16496325] (Human)
• Yesilaltay, A et al. Effects of hepatic expression of the high-density lipoprotein receptor SR-BI on lipoprotein metabolism and female fertility. Endocrinology;147(4):1577-88. 2006 Apr. [PMID: 16410302]
• Harder, CJ et al. Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy. J Lipid Res;47(3):492-503. 2006 Mar. [PMID: 16339112]
• Tondu AL, Robichon C, Yvan-Charvet L et al. Insulin and angiotensin II induce the translocation of scavenger receptor class B, type I from intracellular sites to the plasma membrane of adipocytes. J Biol Chem 2005 Sep 30 [PMID: 16033765] (WB, ICC/IF, FLOW, Mouse)
• Johansson, L et al. Selective thyroid receptor modulation by GC-1 reduces serum lipids and stimulates steps of reverse cholesterol transport in euthyroid mice. Proc Natl Acad Sci U S A;102(29):10297-302. 2005 Jul 19. [PMID: 16006512]
• Barth H, Cerino R, Arcuri M et al. Scavenger receptor class B type I and hepatitis C virus infection of primary tupaia hepatocytes. J Virol;79(9):5774-85. 2005 May. [PMID: 15827192]
• Lalanne, F et al. Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells. J Lipid Res;46(6):1312-9. 2005 Jun. [PMID: 15741654]
• Lemieux, C et al. Hypolipidemic action of the SERM acolbifene is associated with decreased liver MTP and increased SR-BI and LDL receptors. J Lipid Res;46(6):1285-94. 2005 Jun. [PMID: 15741653]
• Brodeur, MR et al. Physiological importance of SR-BI in the in vivo metabolism of human HDL and LDL in male and female mice. J Lipid Res;46(4):687-96. 2005 Apr. [PMID: 15654132]
• Han, J et al. Functional interplay between the macrophage scavenger receptor class B type I and pitavastatin (NK-104). Circulation;110(22):3472-9. 2004 Nov 30. [PMID: 15557368]
• Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol 2003 Dec [PMID: 12952856] (WB)
• Zager RA, Johnson AC, Hanson SY, Shah VO. Acute tubular injury causes dysregulation of cellular cholesterol transport proteins. Am J Pathol 2003 Jul [PMID: 12819036] (WB, Mouse)
• Sehayek E, Wang R, Ono JG et al. Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol. J Lipid Res 2003 Sep [PMID: 12810817] (WB, Mouse)
• Wadsack C, Hrzenjak A, Hammer A et al. Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins. Eur J Biochem 2003 Feb [PMID: 12542695] (ICC/IF, Human)
• Johnson AC, Yabu JM, Hanson S et al. Experimental glomerulopathy alters renal cortical cholesterol, SR-B1, ABCA1, and HMG CoA reductase expression Am J Pathol. 2003 Jan [PMID: 12507911] (WB, Mouse)
• Zager RA, Johnson AC, Hanson SY. Sepsis syndrome stimulates proximal tubule cholesterol synthesis and suppresses the SR-B1 cholesterol transporter. Kidney Int 2003 Jan [PMID: 12472775]
• Scarselli E, Ansuini H, Cerino R et al The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J 2002 Oct 1 [PMID: 12356718] (FLOW, WB, Human, Chinese Hamster)
• Goti, D et al. Effects of lipoprotein lipase on uptake and transcytosis of low density lipoprotein (LDL) and LDL-associated alpha-tocopherol in a porcine in vitro blood-brain barrier model. J Biol Chem;277(32):28537-44. 2002 Aug 9. [PMID: 12032155] (WB, Human)
• Hullinger, Thomas G et al. p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain. J Biol Chem;276(50):46807-14. 2001 Dec 14. [PMID: 11585816]
• Witt, W et al. Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells. J Lipid Res;41(12):2009-16. 2000 Dec. [PMID: 11108734] (WB, Human, Rat)

Reviews for SR-BI Antibody (NB400-104) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Pierre Jean Beltran ICC Human 04/05/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Human fibroblast
• Species: Human

Comments

• Comments: SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% bovine serum albumin. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).

reviewed by: Ryan Russell Flow Human 11/06/2012

Summary

• Application: Flow Cytometry
• Sample Tested: HepG2 liver cells
• Species: Human
• Lot: P-1
• Comments: Less Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.

Blocking

• Blocking Details: 5% milk in TBST

Primary Anitbody

• Dilution Ratio: 1:5000, 5% milk in TBST 4 degrees overnight in

Details

• Detection Notes: PVDF membrane. Thermo Scientific Chemiluminescent Substrate, 5-min. negative control: eNOS; positive control: actin

Comments

• Comments: Less Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.

reviewed by: Mitchell Knutson WB Rat 10/23/2012

Summary

• Application: Western Blot
• Sample Tested: H4IIE total cell lysates and plasma membrane proteins
• Species: Rat
• Lot: P-1

Blocking

• Blocking Details: 5% Non fat dry milk in TBST, 1 h, RT

Primary Anitbody

• Dilution Ratio: 1:2000 in blocking buffer, overnight incubation at 4 degrees

Secondary Antibody

• Secondary Description: HRP-conjugated donkey anti-rabbit igG
• Secondary Manufacturer Cat#: GE healthcare NA9340V
• Secondary Concentration: 1:2000-1:5000

Details

• Detection Notes: West Pico (Pierce), visualized with FluoroChem E (Cell Biosciences)

reviewed by: Anonymous Flow Mouse 11/23/2011

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse
• Species: Mouse
• Comments: The signal is strong and clear

Blocking

• Blocking Details: Blocking Buffer: 1% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 100x, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Details

• Detection Notes: Detection Visualization Method: LSM 700 Confocal Microscopy, Secondary Ab: Goat anti-rabbit IgG FITC

Comments

• Comments: The signal is strong and clear

FAQs for SR-BI Antibody (NB400-104). (Showing 1 - 3 of 3 FAQs).

1. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
   • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
2. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
   • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
3. Do the cells have to be permeabilized before using this antibody for flow cytometry?
   • For flow cytometry, we do recommend fixation and permeabilization of the cell prior to incubation with this antibody. The product NB400-104 recognizes an epitope in the cytoplasmic domain of this membrane protein. Usually our lab will recommend using 0.1-0.5% Tween-20 for membrane permeabilization. Sometimes, you can also achieve ample permeabilization of the membrane using methanol fixation. Our lab's flow protocol can be found here: Flow Cytometry protocol.

Bioinformatics

• Gene Symbol: SCARB1
• Entrez:
  • 949(Human)
  • 20778(Mouse)
  • 25073(Rat)
• Uniprot:
  • Q61009(Mouse)