SR-BI Antibody

Images

 
Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% ...read more
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.
Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Genetic Strategies: Knockdown Validated: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. Silence of CD36 decreased HCV infection and replication (n = 3). Huh7.5 cells were transfected with 0.2 ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

SR-BI Antibody Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:1000
  • Immunohistochemistry 2.5 - 5 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 - 5 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Block/Neutralize
  • Knockdown Validated
  • Knockout Validated
Application Notes
In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in IHC-Frozen reported in scientific literature (PMID: 26865459). Knockout validation (PMID: 31462534). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in blocking/ neutralizing reported in scientific literature (PMID: 24859737).
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SR-BI Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.2
using
NB400-104 in the following applications:

Publications
Read Publications using
NB400-104 in the following applications:

  • BN
    1 publication
  • 7 publications
  • 3 publications
  • 10 publications
  • IHC
    7 publications
  • 1 publication
  • 7 publications
  • IP
    2 publications
  • KO
    1 publication
  • WB
    83 publications

Reactivity Notes

Reactivity with CHO cells reported in scientific literature (PMID: 12356718). Porcine reactivity reported in scientific literature (PMID: 26852751).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SR-BI Antibody (NB400-104)(196)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Chinese Hamster, Golden Syrian Hamster, Hamster, Porcine, Primate, Rabbit.

We have publications tested in 10 applications: BN, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB.


Filter By Application
BN
(1)
FLOW
(7)
Flow
(3)
ICC/IF
(10)
IHC
(7)
IHC-Fr
(1)
IHC-P
(7)
IP
(2)
KO
(1)
WB
(83)
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Filter By Species
Human
(46)
Mouse
(53)
Rat
(14)
Chinese Hamster
(1)
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(1)
Hamster
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 196. Show All 196 Publications.
Publications using NB400-104 Applications Species
Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. May 8 2020 [PMID: 32437644] (WB, Human) WB Human
Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020 May 21 [PMID: 32437392] (WB, Human) WB Human
Mutoh T, Shirai T, Ishii T et Al. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis Nat Commun Mar 9 2020 [PMID: 32152303] (IP, Rat)

Details:
YB2/0
IP Rat
Guo Y MicroRNA-205-5p Promotes Unstable Atherosclerotic Plaque Formation In Vivo Cardiovasc Drugs Ther Feb 8 2020 [PMID: 32034643] (Mouse) Mouse
Ji A, Wang X, Noffsinger VP et al. Serum amyloid A is not incorporated into HDL during HDL biogenesis J. Lipid Res. Jan 8 2020 [PMID: 31915139] (WB, Mouse) WB Mouse
Huang J, Yin H, Yin P et al. SR-BI Interactome Analysis Reveals a Proviral Role for UGGT1 in Hepatitis C Virus Entry Front Microbiol Sep 6 2019 [PMID: 31551978] (WB, Human) WB Human
Zhang J, Qu C, Li T et al. Phagocytosis mediated by scavenger receptor class BI promotes macrophage transition during skeletal muscle regeneration J. Biol. Chem. Aug 28 2019 [PMID: 31462534] (WB, KO, Mouse) WB, KO Mouse
Roscam Abbing RLP, Slijepcevic D, Donkers JM et al. Blocking Sodium-Taurocholate Cotransporting Polypeptide Stimulates Biliary Cholesterol and Phospholipid Secretion in Mice Hepatology Jan 1 2020 [PMID: 31136002] (WB, Mouse) WB Mouse
Wu ZY, Li H, Li JR et al. Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I Eur. J. Pharmacol. Mar 19 2019 [PMID: 30902657] (WB, Human) WB Human
Tsuzuki S, Lee S, Kimoto Y et al. A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae Biomed. Res. Dec 12 2018 [PMID: 30531159] (IHC, Mouse) IHC Mouse
Show All 196 Publications.

Reviews for SR-BI Antibody (NB400-104) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB400-104:
Filter by Applications
ICC
(2)
Flow
(2)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SR-BI NB400-104
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reviewed by:
Hongxue Shi
ICC Mouse 10/02/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse macrophage
SpeciesMouse
LotT-4
Immunocytochemistry SR-BI NB400-104
Enlarge
reviewed by:
Pierre Jean Beltran
ICC Human 04/05/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast
SpeciesHuman

Comments

CommentsSR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% bovine serum albumin. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).
Flow Cytometry SR-BI NB400-104
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reviewed by:
Ryan Russell
Flow Human 11/06/2012
View

Summary

ApplicationFlow Cytometry
Sample TestedHepG2 liver cells
SpeciesHuman
LotP-1
CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1:5000, 5% milk in TBST 4 degrees overnight in

Details

Detection NotesPVDF membrane. Thermo Scientific Chemiluminescent Substrate, 5-min. negative control: eNOS; positive control: actin

Comments

CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.
Western Blot SR-BI NB400-104
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reviewed by:
Mitchell Knutson
WB Rat 10/23/2012
View

Summary

ApplicationWestern Blot
Sample TestedH4IIE total cell lysates and plasma membrane proteins
SpeciesRat
LotP-1

Blocking

Blocking Details5% Non fat dry milk in TBST, 1 h, RT

Primary Anitbody

Dilution Ratio1:2000 in blocking buffer, overnight incubation at 4 degrees

Secondary Antibody

Secondary DescriptionHRP-conjugated donkey anti-rabbit igG
Secondary Manufacturer Cat#GE healthcare NA9340V
Secondary Concentration1:2000-1:5000

Details

Detection NotesWest Pico (Pierce), visualized with FluoroChem E (Cell Biosciences)
 
reviewed by:
Anonymous
Flow Mouse 11/23/2011
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse
SpeciesMouse
CommentsThe signal is strong and clear

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 100x, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Details

Detection NotesDetection Visualization Method: LSM 700 Confocal Microscopy, Secondary Ab: Goat anti-rabbit IgG FITC

Comments

CommentsThe signal is strong and clear

Product General Protocols

View specific protocols for SR-BI Antibody (NB400-104): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104). (Showing 1 - 4 of 4 FAQs).

  1. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  2. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
  3. Do the cells have to be permeabilized before using this antibody for flow cytometry?
    • For flow cytometry, we do recommend fixation and permeabilization of the cell prior to incubation with this antibody. The product NB400-104 recognizes an epitope in the cytoplasmic domain of this membrane protein. Usually our lab will recommend using 0.1-0.5% Tween-20 for membrane permeabilization. Sometimes, you can also achieve ample permeabilization of the membrane using methanol fixation. Our lab's flow protocol can be found here: Flow Cytometry protocol.
  4. Hi. I'm interested in scarb1 antibody. Can you tell me what is the difference between NB400-101 and 104?
    • SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-104AF350
Alexa Fluor 405 NB400-104AF405
Alexa Fluor 488 NB400-104AF488
Alexa Fluor 532 NB400-104AF532
Alexa Fluor 594 NB400-104AF594
Alexa Fluor 647 NB400-104AF647
Alexa Fluor 700 NB400-104AF700
Alexa Fluor 750 NB400-104AF750
Biotin NB400-104B
DyLight 350 NB400-104UV
DyLight 405 NB400-104V
DyLight 488 NB400-104G
DyLight 550 NB400-104R
DyLight 594 NB400-104DL594
DyLight 650 NB400-104C
DyLight 680 NB400-104FR
DyLight 755 NB400-104IR
FITC NB400-104F
HRP NB400-104H
Janelia Fluor 549 NB400-104JF549
Janelia Fluor 646 NB400-104JF646
PE NB400-104PE

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Bioinformatics Tool for SR-BI Antibody (NB400-104)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Hongxue Shi
10/02/2020
Application: ICC
Species: Mouse

Pierre Jean Beltran
04/05/2017
Application: ICC
Species: Human

Ryan Russell
11/06/2012
Application: Flow
Species: Human

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot