SR-BI Antibody - BSA Free

Images

 
Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using ...read more
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% ...read more
Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Genetic Strategies: Knockdown Validated: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. Silence of CD36 decreased HCV infection and replication (n = 3). Huh7.5 cells were transfected with 0.2 ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, B/N, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

SR-BI Antibody - BSA Free Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:1000
  • Immunohistochemistry 2.5 - 5 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 - 5 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Proximity Ligation Assay
  • Simple Western 1:100
  • Western Blot 1:1000 - 1:5000
Application Notes
Use in PLA reported in scientific literature (PMID:10.1016/j.jbc.2021.100828) In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in IHC-Frozen reported in scientific literature (PMID: 26865459). Knockout validation (PMID: 31462534). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in blocking/ neutralizing reported in scientific literature (PMID: 24859737).
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SR-BI Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.2
using
NB400-104 in the following applications:

Publications
Read Publications using
NB400-104 in the following applications:

  • BN
    1 publication
  • 7 publications
  • 3 publications
  • 13 publications
  • IHC
    8 publications
  • 1 publication
  • 7 publications
  • IP
    2 publications
  • KO
    1 publication
  • PLA
    1 publication
  • WB
    95 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33719499).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody - BSA Free

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: B/N, ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
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NBP3-04922
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NBP2-22106
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NB400-104
Species: Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHa
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, B/N, KD, KO

Publications for SR-BI Antibody (NB400-104)(218)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Chinese Hamster, Golden Syrian Hamster, Hamster, Porcine, Primate, Rabbit.

We have publications tested in 11 applications: BN, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, PLA, WB.


Filter By Application
BN
(1)
FLOW
(7)
Flow
(3)
ICC/IF
(13)
IHC
(8)
IHC-Fr
(1)
IHC-P
(7)
IP
(2)
KO
(1)
PLA
(1)
WB
(95)
All Applications
Filter By Species
Human
(51)
Mouse
(64)
Rat
(14)
Chinese Hamster
(1)
Golden Syrian Hamster
(2)
Hamster
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 218. Show All 218 Publications.
Publications using NB400-104 Applications Species
Li L, Zhong S, Li R et al. Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRalpha-mediated ABCA1 expression JCI insight [PMID: 35393951]
Li Q, Liu X, Zhang X et al. Terpene Lactucopicrin Limits Macrophage Foam Cell Formation by a Reduction of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 in Lipid Rafts Molecular nutrition & food research Dec 21 2021 [PMID: 34932892] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Nyandwi JB, Ko YS, Jin H Et al. Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice Biomolecules Oct 6 2021 [PMID: 34680102] (WB, Mouse) WB Mouse
Haberl EM, Pohl R, Rein-Fischboeck L Et al. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice Lipids in health and disease Oct 10 2021 [PMID: 34629057] (WB, Mouse) WB Mouse
Raith M, Kauffman S, Asoudeh M Et Al. Elongated PEO-based nanoparticles bind the high-density lipoprotein (HDL) receptor scavenger receptor class B I (SR-BI) Journal of Controlled Release Aug 1 2021 [PMID: 34352314] (ICC/IF) ICC/IF
Zapatero-Belinchon Fj, Otjengerdes R, Sheldon J Et Al. Interdependent Impact of Lipoprotein Receptors and Lipid-Lowering Drugs on HCV Infectivity Cells Jun 29 2021 [PMID: 34209751] (WB) WB
Kulkarni NN, O'Neill AM, Dokoshi T et al. Sequence determinants in the cathelicidin LL37 that promote inflammation via presentation of RNA to scavenger receptors The Journal of biological chemistry May 25 2021 [PMID: 34048712] (PLA, Human) PLA Human
Zhang Z, Zhou Q, Liu R et al. The adaptor protein GIPC1 stabilizes the scavenger receptor SR-B1 and increases its cholesterol uptake Journal of Biological Chemistry Mar 1 2021 [PMID: 33811857] (Human) Human
Li X, Lu Q, Qiu Y et al. Direct Cardiac Actions of the Sodium Glucose Co-Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure-Overload Heart Failure Journal of the American Heart Association Mar 16 2021 [PMID: 33719499] (WB, Mouse) WB Mouse
Morton RE, Mihna D, Liu Y The lipid substrate preference of CETP controls the biochemical properties of HDL in fat/cholesterol-fed hamsters Journal of lipid research Jan 27 2021 [PMID: 33515552] (WB, Golden Syrian Hamster) WB Golden Syrian Hamster
Show All 218 Publications.

Reviews for SR-BI Antibody (NB400-104) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB400-104:
Filter by Applications
Immunocytochemistry
(2)
Flow Cytometry
(2)
Western Blot
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SR-BI NB400-104
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4
reviewed by:
Verified Customer
Immunocytochemistry Mouse 10/02/2020
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Summary

ApplicationImmunocytochemistry
Sample Testedmouse macrophage
SpeciesMouse
LotT-4
Immunocytochemistry SR-BI NB400-104
Enlarge
4
reviewed by:
Verified Customer
Immunocytochemistry Human 04/05/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast
SpeciesHuman

Comments

CommentsSR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% bovine serum albumin. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).
Flow Cytometry SR-BI NB400-104
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4
reviewed by:
Verified Customer
Flow Cytometry Human 11/06/2012
View

Summary

ApplicationFlow Cytometry
Sample TestedHepG2 liver cells
SpeciesHuman
LotP-1
Western Blot SR-BI NB400-104
Enlarge
5
reviewed by:
Verified Customer
Western Blot Rat 10/23/2012
View

Summary

ApplicationWestern Blot
Sample TestedH4IIE total cell lysates and plasma membrane proteins
SpeciesRat
LotP-1
  4
reviewed by:
B.M. Tao
Flow Cytometry Mouse 11/23/2011
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse
SpeciesMouse

Product General Protocols

View specific protocols for SR-BI Antibody (NB400-104): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104). (Showing 1 - 4 of 4 FAQs).

  1. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  2. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
  3. Do the cells have to be permeabilized before using this antibody for flow cytometry?
    • For flow cytometry, we do recommend fixation and permeabilization of the cell prior to incubation with this antibody. The product NB400-104 recognizes an epitope in the cytoplasmic domain of this membrane protein. Usually our lab will recommend using 0.1-0.5% Tween-20 for membrane permeabilization. Sometimes, you can also achieve ample permeabilization of the membrane using methanol fixation. Our lab's flow protocol can be found here: Flow Cytometry protocol.
  4. Hi. I'm interested in scarb1 antibody. Can you tell me what is the difference between NB400-101 and 104?
    • SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SR-BI Antibody (NB400-104)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

Omicron Variant Antibodies

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Verified Customer
10/02/2020
Application: Immunocytochemistry
Species: Mouse

Verified Customer
04/05/2017
Application: Immunocytochemistry
Species: Human

Verified Customer
11/06/2012
Application: Flow Cytometry
Species: Human

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot