SR-BI Antibody

Images

 
Simple Western: SR-BI Antibody [NB400-104] - Simple Western lane view shows a specific band for SR-BI in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1,000 dilution, shows distinct band at 82 kDa.
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% ...read more
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and ...read more
Immunohistochemistry: SR-BI Antibody [NB400-104] - Staining of adrenal cortex.
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104PE (blue) and a matched isotype control NBP2-24893PE (orange). Cells were fixed with 4% PFA ...read more
Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy ...read more
Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.
Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis using the DyLight 550 conjugate of NB400-104. SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SR-BI Antibody Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 2.5-5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5-5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26865459).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
SR-BI Lysate (NBL1-15720)
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB400-104 in the following applications:

Publications
Read Publications using
NB400-104 in the following applications:

Reactivity Notes

Mouse, rat, mink, hamster, primate, rabbit and the long form of human SR-BI. Reactivity with CHO cells reported in scientific literature (PMID: 12356718).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-104
Species: Hu, Mu, Rt, ChHa, Ha, Md, Pm, Rb, SyHa
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for SR-BI Antibody (NB400-104)(151)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Chinese Hamster, Golden Syrian Hamster, Hamster, Porcine, Primate, Rabbit.

We have publications tested in 7 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


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FLOW
(8)
ICC/IF
(8)
IHC
(6)
IHC-Fr
(1)
IHC-P
(6)
IP
(1)
WB
(57)
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Human
(31)
Mouse
(29)
Rat
(11)
Chinese Hamster
(1)
Golden Syrian Hamster
(1)
Hamster
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 151. Show All 151 Publications.
Publications using NB400-104 Applications Species
Durham K, Chathely K, Mak KC et al. HDL protects against doxorubicin induced cardiotoxicity in a scavenger receptor class B type 1, PI3K, and AKT dependent manner.. Am. J. Physiol. Heart Circ. Physiol. 2017 Oct 06 [PMID: 28986362] (ICC/IF, Human) ICC/IF Human
Qian Y, Qiao S, Dai Y et al. Molecular-Targeted Immunotherapeutic Strategy for Melanoma via Dual-Targeting Nanoparticles Delivering Small Interfering RNA to Tumor-Associated Macrophages ACS Nano 2017 Sep 26 [PMID: 28858473] (WB, Mouse) WB Mouse
Russelli G, Pizzillo P, Iannolo G et al. HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation PLoS ONE 2017 Jul 27 [PMID: 28750044] (ICC/IF, Human) ICC/IF Human
Castella B, Kopecka J, Sciancalepore P et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vg9Vdelta2 T cell activation by dendritic cells. Nat Commun. 2017 Jun 05 [PMID: 28580927] (WB, Human) WB Human
Fletcher RB, Das D, Gadye L et al. Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution. Cell Stem Cell Apr 28 2017 [PMID: 28506465]
Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human) WB Human
Geng S, Chen K, Yuan R et al. The persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis. Nat Commun. Nov 8 2016 [PMID: 27824038] (Mouse) Mouse
Cedo L, Garcia-Leon A, Baila-Rueda L et al. ApoA-I mimetic administration, but not increased apoA-I-containing HDL, inhibits tumour growth in a mouse model of inherited breast cancer. Sci Rep. Nov 3 2016 [PMID: 27808249] (WB, Mouse) WB Mouse
Pierre J, Rommel M, Ileana C. A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection. Cell Systems 2016 Oct 26 [PMID: 27641956] (ICC/IF, Human) ICC/IF Human
Scharinger B, Messner B, Turkcan A et al. Leoligin, the major lignan from Edelweiss, inhibits 3-hydroxy-3-methyl-glutaryl-CoA reductase and reduces cholesterol levels in ApoE-/- mice J Mol Cell Cardiol 2016 Oct [PMID: 27497529] (WB)

Details:
This reference used the HRP version of NB400-104.
WB
Show All 151 Publications.

Reviews for SR-BI Antibody (NB400-104) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.
We have 4 reviews tested in 3 application: ICC, Flow, WB.

Reviews using NB400-104:
Filter by Applications
ICC
(1)
Flow
(2)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SR-BI NB400-104
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reviewed by:
Pierre Jean Beltran
ICC Human 04/05/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast
SpeciesHuman

Comments

CommentsSR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation was performed overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% bovine serum albumin. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).
Flow Cytometry SR-BI NB400-104
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reviewed by:
Ryan Russell
Flow Human 11/06/2012
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Summary

ApplicationFlow Cytometry
Sample TestedHepG2 liver cells
SpeciesHuman
LotP-1
CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1:5000, 5% milk in TBST 4 degrees overnight in

Details

Detection NotesPVDF membrane. Thermo Scientific Chemiluminescent Substrate, 5-min. negative control: eNOS; positive control: actin

Comments

CommentsLess Novus anti SR-B1 was required (yielding a stronger signal) than Invitrogen anti SR-B1. Both Novus and Invitrogen antibodies detected over-expression of human SR-B1 in HepG2 cells, but Novus also detected endogenous SR-B1 under the same testing conditions.
Western Blot SR-BI NB400-104
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reviewed by:
Mitchell Knutson
WB Rat 10/23/2012
View

Summary

ApplicationWestern Blot
Sample TestedH4IIE total cell lysates and plasma membrane proteins
SpeciesRat
LotP-1

Blocking

Blocking Details5% Non fat dry milk in TBST, 1 h, RT

Primary Anitbody

Dilution Ratio1:2000 in blocking buffer, overnight incubation at 4 degrees

Secondary Antibody

Secondary DescriptionHRP-conjugated donkey anti-rabbit igG
Secondary Manufacturer Cat#GE healthcare NA9340V
Secondary Concentration1:2000-1:5000

Details

Detection NotesWest Pico (Pierce), visualized with FluoroChem E (Cell Biosciences)
 
reviewed by:
Anonymous
Flow Mouse 11/23/2011
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse
SpeciesMouse
CommentsThe signal is strong and clear

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 100x, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Details

Detection NotesDetection Visualization Method: LSM 700 Confocal Microscopy, Secondary Ab: Goat anti-rabbit IgG FITC

Comments

CommentsThe signal is strong and clear

Product General Protocols

View specific protocols for SR-BI Antibody (NB400-104): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104). (Showing 1 - 3 of 3 FAQs).

  1. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  2. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
  3. Do the cells have to be permeabilized before using this antibody for flow cytometry?
    • For flow cytometry, we do recommend fixation and permeabilization of the cell prior to incubation with this antibody. The product NB400-104 recognizes an epitope in the cytoplasmic domain of this membrane protein. Usually our lab will recommend using 0.1-0.5% Tween-20 for membrane permeabilization. Sometimes, you can also achieve ample permeabilization of the membrane using methanol fixation. Our lab's flow protocol can be found here: Flow Cytometry protocol.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-104AF405
Alexa Fluor 488 Labeled NB400-104AF488
Alexa Fluor 647 Labeled NB400-104AF647
Alexa Fluor 700 Labeled NB400-104AF700
Biotin Labeled NB400-104B
DyLight 405 Labeled NB400-104V
DyLight 488 Labeled NB400-104G
DyLight 550 Labeled NB400-104R
DyLight 650 Labeled NB400-104C
FITC Labeled NB400-104F
HRP Labeled NB400-104H
PE Labeled NB400-104PE

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Bioinformatics Tool for SR-BI Antibody (NB400-104)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Pierre Jean Beltran
04/05/2017
Application: ICC
Species: Human

Ryan Russell
11/06/2012
Application: Flow
Species: Human

Mitchell Knutson
10/23/2012
Application: WB
Species: Rat

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot