Raw 264.7 Whole Cell - T0901317 treated Assay Kit (NBP1-30159)

Images

 
Western Blot: Raw 264.7 Whole Cell - T0901317 treated Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).

Product Details

Summary
Applications WB
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Order Details

Raw 264.7 Whole Cell - T0901317 treated Assay Kit Summary

Localization
Macrophage
Marker
(T0901317 treated)
Kit Type
Assay Kit

Applications/Dilutions

Dilutions
  • Western Blot 0.01ml per lane
Application Notes
For western blot 10ul should be loaded per lane.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Details for Raw 264.7 Whole Cell - T0901317 treated

Type
Cell
Life Stage
Embryonic
Tissue
Blood Lymph
Protein State
Denatured
Tissue Condition
Tumor
Subcellular Fraction
Whole

Kit Components

Notes

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 10% prior to use. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Samples should be heated to 65C for 15 minutes prior to loading.

Background

T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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