Apolipoprotein E/ApoE Antibody (WUE-4)

Images

 
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # ...read more
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark ...read more
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype ...read more
Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody (WUE-4) NB110-60531AF700 (blue) and a matched isotype ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody (WUE-4) NB110-60531PE (blue) and a matched isotype control ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
WUE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Apolipoprotein E/ApoE Antibody (WUE-4) Summary

Immunogen
Purified human ApoE [UniProt# P02649]
Epitope
Residues 140-160 of purified human ApoE (LDLR receptor binding site).
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26564908). This antibody is CyTOF ready.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Apolipoprotein E/ApoE Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-60531 in the following applications:

Publications
Read Publications using
NB110-60531 in the following applications:

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein E/ApoE Antibody (NB110-60531)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, Flow, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(7)
Flow
(1)
ICC/IF
(1)
IHC-P
(1)
WB
(7)
All Applications
Filter By Species
Human
(14)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB110-60531 Applications Species
Schmid B, Prehn KR, Nimsanor N et al. Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. Stem Cell Res Jan 4 2019 [PMID: 30660866] (WB, Human) WB Human
Zhao N, Liu CC, Van Ingelgom AJ et al. APOE epsilon 2 is associated with increased tau pathology in primary tauopathy. Nat Commun. 2018 Oct 22 [PMID: 30348994] (ELISA, Human) ELISA Human
Alvarez P, Genre F, Iglesias M et al. Modulation of autoimmune arthritis severity in mice by apolipoprotein E (ApoE) and cholesterol Clin. Exp. Immunol. 2016 Dec 01 (ELISA, Mouse) ELISA Mouse
Yamazaki Y, Shinohara M, Yamazaki A et al. ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components Arterioscler. Thromb. Vasc. Biol. Jan 1 2020 [PMID: 31665905] (ELISA, Human) ELISA Human
Chouinard-Watkins R, Pincon A, Coulombe JD et al. A Diet Rich in Docosahexaenoic Acid Restores Liver Arachidonic Acid and Docosahexaenoic Acid Concentrations in Mice Homozygous for the Human Apolipoprotein E e4 Allele. J. Nutr. Jun 15 2016 [PMID: 27306896] (WB, Human) WB Human
Yamazaki Y, Liu C, Yamazaki A et al. Vascular apoE4 Impairs Brain Cognition by Modulating Glio-Vascular Functions SSRN Journal Jan 4 2020
Zimmerman KA, Bentley MR, Lever JM et al. Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species. J Am Soc Nephrol. 2019 Apr 4 [PMID: 30948627] (Flow, Human) Flow Human
Liu CC, Zhao N, Fu Y et al. ApoE4 Accelerates Early Seeding of Amyloid Pathology. Neuron. 2017 Dec 6 [PMID: 29216449] (WB, Human) WB Human
Huynh TV, Liao F, Francis CM et al. Age-Dependent Effects of apoE Reduction Using Antisense Oligonucleotides in a Model of beta-amyloidosis. Neuron. 2017 Dec 6 [PMID: 29216448] (IHC-P, Human) IHC-P Human
Zhao N, Liu CC, Van Ingelgom AJ et al. Apolipoprotein E4 Impairs Neuronal Insulin Signaling by Trapping Insulin Receptor in the Endosomes. Neuron. 2017 Sep 27 [PMID: 28957663] (Human) Human
Show All 18 Publications.

Reviews for Apolipoprotein E/ApoE Antibody (NB110-60531) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB110-60531:
Filter by Applications
ELISA
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 09/08/2015
View

Summary

ApplicationELISA
Sample TestedHuman umbilical vein cell line EA.hy926 whole cell lysate after incubation with recombinant human apoE
SpeciesHuman

Blocking

Blocking Details10% nonfat dry milk in PBS/0.05% Tween 20, 1h, 37C

Secondary Antibody

Secondary DescriptionGoat anti-human apoE-HRP

Details

Detection NotesOPD substate, plate reader, measurement of absorbance
Western Blot Apolipoprotein E/ApoE NB110-60531
Enlarge
reviewed by:
Anonymous
WB Human 02/24/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

View specific protocols for Apolipoprotein E/ApoE Antibody (NB110-60531): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apolipoprotein E/ApoE Antibody (NB110-60531). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in purchasing your apolipoprotein E antibody ID: NB110-60531. I would like to ask you which isotype control antibody you would recommend to go along with this antibody based on.
    • Our ApoE (WUE-4) antibody with catalogue number NB110-60531 is a mouse monoclonal of the isotype IgG1 kappa. The following product is an unconjugated mouse IgG1 kappa isotype control which should meet your requirements, however please note isotype control catalog # NBP1-43319 has not been validated in an identical range of applications to ApoE antibody NB110-60531.
  2. I've ordered your ApoE Ab (Apolipoprotein E/ApoE Antibody (WUE-4); NB110-60531) to use for FACS, and one of your images reports that you just fixed with 4% PFA and then used 0.1% saponin. Is the saponin therefore sufficient/preferable for permeabilization (rather than using one of the commercially available fixation and permeabilization kits)?Presumably, the 0.1% saponin then needs to be added to the FACS buffer throughout until you the samples are run, to keep them permeabilized?
    • FLOW has worked with the protocol below and was successful. So we can say it worked but will not say it is the only protocol you should use. We cannot comment on the commercial kit you are referring to since we have not tested directly but assume any protocol that correctly identifies the location of the target protein should be successful.https://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60531AF350
Alexa Fluor 405 NB110-60531AF405
Alexa Fluor 488 NB110-60531AF488
Alexa Fluor 532 NB110-60531AF532
Alexa Fluor 594 NB110-60531AF594
Alexa Fluor 647 NB110-60531AF647
Alexa Fluor 700 NB110-60531AF700
Alexa Fluor 750 NB110-60531AF750
Allophycocyanin NB110-60531APC
Biotin NB110-60531B
DyLight 350 NB110-60531UV
DyLight 405 NB110-60531V
DyLight 488 NB110-60531G
DyLight 550 NB110-60531R
DyLight 594 NB110-60531DL594
DyLight 650 NB110-60531C
DyLight 680 NB110-60531FR
DyLight 755 NB110-60531IR
FITC NB110-60531F
HRP NB110-60531H
Janelia Fluor 549 NB110-60531JF549
Janelia Fluor 646 NB110-60531JF646
PE NB110-60531PE
PerCP NB110-60531PCP

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
09/08/2015
Application: ELISA
Species: Human

Anonymous
02/24/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol APOE
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