Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

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Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # ...read more
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark ...read more
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex ...read more
Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
WUE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free Summary

Immunogen
Purified human ApoE [UniProt# P02649]
Epitope
Residues 140-160 of purified human ApoE (LDLR receptor binding site).
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
Application Notes
This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26564908). This antibody is CyTOF ready.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Apolipoprotein E/ApoE Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-60531 in the following applications:

Publications
Read Publications using
NB110-60531 in the following applications:

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein E/ApoE Antibody (NB110-60531)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, Flow, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(8)
Flow
(3)
ICC/IF
(3)
IHC-P
(1)
WB
(9)
All Applications
Filter By Species
Human
(18)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB110-60531 Applications Species
Jin Y, Li F, Sonoustoun B et al. APOE4 exacerbates alpha-synuclein seeding activity and contributes to neurotoxicity in Alzheimer's disease with Lewy body pathology Acta neuropathologica Apr 26 2022 [PMID: 35471463] (ELISA, Human) ELISA Human
Milich LM, Choi JS, Ryan CB et al. Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord J Exp Med Jun 16 2021 [PMID: 34132743]
Yamazaki Y, Shinohara M, Yamazaki A et al. ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components Arterioscler. Thromb. Vasc. Biol. Jan 1 2020 [PMID: 31665905] (ELISA, Human) ELISA Human
Konings S The synaptic and neurobiological role of apolipoprotein E4 in models of Alzheimer's disease Thesis Jan 1 2022 (WB, Human) WB Human
Shim H, Horner J, Wu C et al. Telomerase reverse transcriptase preserves neuron survival and cognition in Alzheimer s disease models Nature Aging Dec 1 2021 [PMID: 35036927]
Wang G, Yin W, Shin H Et al. Neuronal accumulation of peroxidated lipids promotes demyelination and neurodegeneration through the activation of the microglial NLRP3 inflammasome Nature Aging Nov 1 2021
Gnanaguru G, Wagschal A, Oh J et al. Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration Molecular therapy : the journal of the American Society of Gene Therapy Mar 17 2021 [PMID: 33744470]
Schmid B, Holst B, Clausen C et al. Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (epsilon2/epsilon3/epsilon4) and knock-out for the study of APOE biology in health and disease Stem cell research Feb 2 2021 [PMID: 33556820] (ICC/IF, Flow) ICC/IF, Flow
Yamazaki Y, Liu CC, Yamazaki A et al. Vascular ApoE4 Impairs Behavior by Modulating Gliovascular Function Neuron Dec 4 2020 [PMID: 33321072]
Yang R, Zhao Y, Gu Y et al. Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma Oncogene Aug 27 2020 [PMID: 32855525] (WB) WB
Show All 29 Publications.

Reviews for Apolipoprotein E/ApoE Antibody (NB110-60531) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB110-60531:
Filter by Applications
ELISA
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Angeliki Chroni
ELISA Human 09/08/2015
View

Summary

ApplicationELISA
Sample TestedHuman umbilical vein cell line EA.hy926 whole cell lysate after incubation with recombinant human apoE
SpeciesHuman
Western Blot Apolipoprotein E/ApoE NB110-60531
Enlarge
4
reviewed by:
Hua Jiang
WB Human 02/24/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apolipoprotein E/ApoE Antibody (NB110-60531). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in purchasing your apolipoprotein E antibody ID: NB110-60531. I would like to ask you which isotype control antibody you would recommend to go along with this antibody based on.
    • Our ApoE (WUE-4) antibody with catalogue number NB110-60531 is a mouse monoclonal of the isotype IgG1 kappa. The following product is an unconjugated mouse IgG1 kappa isotype control which should meet your requirements, however please note isotype control catalog # NBP1-43319 has not been validated in an identical range of applications to ApoE antibody NB110-60531.
  2. I've ordered your ApoE Ab (Apolipoprotein E/ApoE Antibody (WUE-4); NB110-60531) to use for FACS, and one of your images reports that you just fixed with 4% PFA and then used 0.1% saponin. Is the saponin therefore sufficient/preferable for permeabilization (rather than using one of the commercially available fixation and permeabilization kits)?Presumably, the 0.1% saponin then needs to be added to the FACS buffer throughout until you the samples are run, to keep them permeabilized?
    • FLOW has worked with the protocol below and was successful. So we can say it worked but will not say it is the only protocol you should use. We cannot comment on the commercial kit you are referring to since we have not tested directly but assume any protocol that correctly identifies the location of the target protein should be successful.https://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Apolipoprotein E/ApoE Products

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Angeliki Chroni
09/08/2015
Application: ELISA
Species: Human

Hua Jiang
02/24/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol APOE