Apolipoprotein E/ApoE Antibody (WUE-4)

Images

 
Western Blot: ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # ...read more
Flow Cytometry: ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Western Blot: ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
WUE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Apolipoprotein E/ApoE Antibody (WUE-4) Summary

Immunogen
Purified human ApoE [UniProt# P02649]
Epitope
Residues 140-160 of purified human ApoE (LDLR receptor binding site).
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26564908)
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Apolipoprotein E/ApoE Lysate (NBL1-07621)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-60531 in the following applications:

Publications
Read Publications using
NB110-60531 in the following applications:

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein E/ApoE Antibody (NB110-60531)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


Filter By Application
ELISA
(4)
ICC/IF
(1)
WB
(5)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB110-60531 Applications Species
Zhao N, Liu CC, Van Ingelgom AJ et al. Apolipoprotein E4 Impairs Neuronal Insulin Signaling by Trapping Insulin Receptor in the Endosomes. Neuron. 2017 Sep 27 [PMID: 28957663] (Human) Human
Chouinard-Watkins R, Pincon A, Coulombe JD et al. A Diet Rich in Docosahexaenoic Acid Restores Liver Arachidonic Acid and Docosahexaenoic Acid Concentrations in Mice Homozygous for the Human Apolipoprotein E e4 Allele. J. Nutr. Jun 15 2016 [PMID: 27306896] (WB, Human) WB Human
Pincon A, Coulombe JD, Chouinard-Watkins R, Plourde M. Human apolipoprotein E allele and docosahexaenoic acid intake modulate peripheral cholesterol homeostasis in mice. J. Nutr. Biochem. May 14 2016 [PMID: 27239755] (WB, Mouse) WB Mouse
Jung BK, Kim HR, Park GN et al. Roles of human apolipoprotein E in the infectivity and replication of hepatitis C virus genotype 2a. J. Microbiol. Jun 1 2016 [PMID: 27225463] (Human) Human
Tambini MD, Pera M, Kanter E et al. ApoE4 upregulates the activity of mitochondria-associated ER membranes. EMBO Rep. 2015 Nov 12 [PMID: 26564908] (WB, ICC/IF) WB, ICC/IF
Fukuhara T, Wada M, Nakamura S et al. Amphipathic alpha-Helices in Apolipoproteins Are Crucial to the Formation of Infectious Hepatitis C Virus Particles. PLoS Pathog. 2014 Dec 01 [PMID: 25502789] (WB, ELISA, Human)

Details:
ApoE antibody was used for Western blot and ELISA in ApoE-knockout Huh7 cell line to confirm deficiencies of ApoE expression (Figure S1).
WB, ELISA Human
Lee CY, Tse W, Smith JD, Landreth GE. Apolipoprotein E Promotes beta-Amyloid Trafficking and Degradation by Modulating Microglial Cholesterol Levels. J Biol Chem;287(3):2032-44. 2012 Jan 13. [PMID: 22130662] (ELISA, Mouse) ELISA Mouse
Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Lutjohann D, Pritchard H, Wellington CL. LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins. J Lipid Res;50(5):885-93. 2009 May. [PMID: 19065001] (ELISA, Mouse) ELISA Mouse
Fryer JD et al. The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice. J Biol Chem;280(27):25754-9. 2005 Jul 8. [PMID: 15888448] (ELISA, Human) ELISA Human
Krul ES, Tikkanen MJ, Schonfeld G. Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function. J Lipid Res;29(10):1309-25. 1988 Oct. [PMID: 2466929] (WB, Human) WB Human

Reviews for Apolipoprotein E/ApoE Antibody (NB110-60531) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: ELISA, WB.

Reviews using NB110-60531:
Filter by Applications
ELISA
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 09/08/2015
View

Summary

ApplicationELISA
Sample TestedHuman umbilical vein cell line EA.hy926 whole cell lysate after incubation with recombinant human apoE
SpeciesHuman

Blocking

Blocking Details10% nonfat dry milk in PBS/0.05% Tween 20, 1h, 37C

Secondary Antibody

Secondary DescriptionGoat anti-human apoE-HRP

Details

Detection NotesOPD substate, plate reader, measurement of absorbance
Western Blot Apolipoprotein E/ApoE NB110-60531
Enlarge
reviewed by:
Anonymous
WB Human 02/24/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

View specific protocols for Apolipoprotein E/ApoE Antibody (NB110-60531): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apolipoprotein E/ApoE Antibody (NB110-60531). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in purchasing your apolipoprotein E antibody ID: NB110-60531. I would like to ask you which isotype control antibody you would recommend to go along with this antibody based on.
    • Our ApoE (WUE-4) antibody with catalogue number NB110-60531 is a mouse monoclonal of the isotype IgG1 kappa. The following product is an unconjugated mouse IgG1 kappa isotype control which should meet your requirements, however please note isotype control catalog # NBP1-43319 has not been validated in an identical range of applications to ApoE antibody NB110-60531.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-60531AF405
Alexa Fluor 488 Labeled NB110-60531AF488
Alexa Fluor 647 Labeled NB110-60531AF647
Alexa Fluor 700 Labeled NB110-60531AF700
Biotin Labeled NB110-60531B
DyLight 405 Labeled NB110-60531V
DyLight 488 Labeled NB110-60531G
DyLight 550 Labeled NB110-60531R
DyLight 650 Labeled NB110-60531C
FITC Labeled NB110-60531F
HRP Labeled NB110-60531H

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
09/08/2015
Application: ELISA
Species: Human

Anonymous
02/24/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol APOE
Entrez