ABCA1 Antibody [DyLight 550] Summary
Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot
- Flow Cytometry
This ABCA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence (PMID: 21501868), Western blot and Immunohistochemistry on frozen and paraffin embedded sections. In Western blot a band representing ABCA1 is observed at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. ELISA has been reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Canine reactivity reported in scientific literature (PMID: 24612239)
Packaging, Storage & Formulations
Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for ABCA1 Antibody [DyLight 550]
- ABC1ATP-binding cassette transporter A1
- ATP-binding cassette 1
- ATP-binding cassette transporter 1
- ATP-binding cassette, sub-family A (ABC1), member 1
- Cholesterol efflux regulatory protein
- EC 18.104.22.168
- EC 22.214.171.124
- EC 3.6.3
- EC 126.96.36.199
- TGDATP-binding cassette sub-family A member 1
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for ABCA1 Antibody (NB400-105R). (Showing 1 - 1 of 1 FAQ).
May I just confirm that NB400-105C, NB400-105G, and NB400-105R are all raised against the same immunogen and that they are simply conjugated to different DyLights? Also, the customer has asked if you might know if the actual immunogen is located in the intra or extracellular domain. If unknown was the flow performed on only fixed/permeabilized cells or also on fresh/unfixed? Do you have any examples of flow staining?
- You are correct, NB400-105C, NB400-105G, and NB400-105R are all NB400-105 conjugated to different fluorochromes. The immunogen is located in the intracellular domain. We always fix and permeabilize our cells for flow, regardless of immunogen. With this being intracellular, it will be necessary. Unfortunately, I do not have any examples of flow data at the moment. It may have come from customer feedback.
Other Available Formats
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Blogs on ABCA1. Showing 1-10 of 12 blog posts - Show all blog posts.
Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post.
ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr... Read full blog post.
ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp... Read full blog post.
ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito... Read full blog post.
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues... Read full blog post.
SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g.... Read full blog post.
SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP... Read full blog post.
LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee... Read full blog post.
The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa... Read full blog post.
Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th... Read full blog post.