ABCG1 Antibody

Images

 
Biological Strategies: Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. 1: untreated mouse peritoneal macrophages, 2: 22-(R)-hydrocholesterol treated macrophages, 3: ...read more
Immunohistochemistry-Paraffin: ABCG1 Antibody [NB400-132] - Staining of human lung, alveolar macrophages.
Flow (Intracellular): ABCG1 Antibody [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with ...read more
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.
Western Blot: ABCG1 Antibody [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Ha, Mk, RbSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

ABCG1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:400
  • Immunohistochemistry-Paraffin 1:100 - 1:400
  • In vitro assay
  • Flow (Intracellular)
Application Notes
Preliminary results suggest that this antibody does not work in IP. In vivo assay was reported in scientific literature. Use in ICC/IF reported in scientific literature (PMID 23185679)
Control Peptide
Reviewed Applications
Read 2 Reviews rated 5
using
NB400-132 in the following applications:

Publications
Read Publications using
NB400-132 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID 27230131).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCG1 Antibody

  • ABC transporter 8
  • ABC8
  • ABC8ATP-binding cassette sub-family G member 1
  • ABCG1
  • ATP-binding cassette transporter 8
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • homolog of Drosophila white
  • MGC34313
  • white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G
  • White protein homolog
  • WHITE1
  • WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 involves in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-30032
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NB400-132
Species: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, In vitro

Publications for ABCG1 Antibody (NB400-132)(118)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chinese Hamster, Hamster, Monkey, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ICC/IF
(2)
IHC
(4)
IHC-P
(1)
In vitro
(1)
WB
(61)
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Filter By Species
Human
(22)
Mouse
(46)
Rat
(3)
Chinese Hamster
(1)
Hamster
(1)
Monkey
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 118. Show All 118 Publications.
Publications using NB400-132 Applications Species
Sedgeman LR, Beysen C, Ramirez Solano MA et al. Beta cell secretion of miR-375 to HDL is inversely associated with insulin secretion Sci Rep Mar 7 2019 [PMID: 30846744] (WB, Mouse) WB Mouse
Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019 Jan [PMID: 30389579] (Mouse) Mouse
Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse) WB Mouse
Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human) WB Human
Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018 Jun 15 [PMID: 29906247] (Mouse) Mouse
Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim. Biophys. Acta 2018 Jun 08 [PMID: 29890224] (WB, Mouse) WB Mouse
Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim. Biophys. Acta 2018 Jun 08 [PMID: 29890224] (WB, Mouse) WB Mouse
Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018 May 26 [PMID: 29802792] (Human) Human
Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor-kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017 Oct 31 [PMID: 29375683] (WB, Human) WB Human
Cuffe H, Liu M, Key CC et al. Targeted Deletion of Adipocyte Abca1 (ATP-Binding Cassette Transporter A1) Impairs Diet-Induced Obesity Arterioscler. Thromb. Vasc. Biol. 2018 Jan 18 [PMID: 29348118] (Mouse) Mouse
Show All 118 Publications.

Reviews for ABCG1 Antibody (NB400-132) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB400-132:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Angeliki Chroni
WB Human 10/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
SpeciesHuman
Western Blot ABCG1 NB400-132
Enlarge
reviewed by:
Anonymous
WB Mouse 08/26/2013
View

Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsExcellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
Pub Med ID23826352

Product General Protocols

View specific protocols for ABCG1 Antibody (NB400-132): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG1 Antibody (NB400-132) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-132AF405
Alexa Fluor 488 Labeled NB400-132AF488
Alexa Fluor 647 Labeled NB400-132AF647
Alexa Fluor 700 Labeled NB400-132AF700
Biotin Labeled NB400-132B
DyLight 405 Labeled NB400-132V
DyLight 488 Labeled NB400-132G
DyLight 550 Labeled NB400-132R
DyLight 650 Labeled NB400-132C
FITC Labeled NB400-132F
HRP Labeled NB400-132H

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Bioinformatics Tool for ABCG1 Antibody (NB400-132)

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Research Areas for ABCG1 Antibody (NB400-132)

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Blogs on ABCG1.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG1: Easy as 123
ABCG1 (ATP-binding cassette sub-family G member 1) is a transporter protein that is primarily involved in macrophage lipid homeostasis. It is a member of the superfamily of ATP-binding cassette (ABC) transporters and localizes to intracellular compart...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

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Recent Reviews

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Angeliki Chroni
10/08/2014
Application: WB
Species: Human

Anonymous
08/26/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ABCG1