ABCG1 Antibody

Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. 1: untreated mouse peritoneal macrophages, 2: 22-(R)-hydrocholesterol treated macrophages, 3: T0901713 treated macrophages, 4: T091713 treated macrophages.

Immunohistochemistry-Paraffin: ABCG1 Antibody [NB400-132] - Staining of human lung, alveolar macrophages.

Flow (Intracellular): ABCG1 Antibody [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody (SA5-10033, Thermo Fisher).

Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.

Western Blot: ABCG1 Antibody [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.

• Reactivity: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, In vitro
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ABCG1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:400
  • Immunohistochemistry-Paraffin 1:100 - 1:400
  • In vitro assay
  • Flow (Intracellular)
• Application Notes: Preliminary results suggest that this antibody does not work in IP. In vivo assay was reported in scientific literature. Use in ICC/IF reported in scientific literature (PMID 23185679)

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID 27230131).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCG1 Antibody

• ABC transporter 8
• ABC8
• ABC8ATP-binding cassette sub-family G member 1
• ABCG1
• ATP-binding cassette transporter 8
• ATP-binding cassette, sub-family G (WHITE), member 1
• homolog of Drosophila white
• MGC34313
• white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G
• White protein homolog
• WHITE1
• WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 involves in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG1 Antibody (NB400-132)(118)

Publications using NB400-132

• Sedgeman LR, Beysen C, Ramirez Solano MA et al. Beta cell secretion of miR-375 to HDL is inversely associated with insulin secretion Sci Rep Mar 7 2019 [PMID: 30846744] (WB, Mouse)
• Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019 Jan [PMID: 30389579] (Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human)
• Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018 Jun 15 [PMID: 29906247] (Mouse)
• Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim. Biophys. Acta 2018 Jun 08 [PMID: 29890224] (WB, Mouse)
• Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim. Biophys. Acta 2018 Jun 08 [PMID: 29890224] (WB, Mouse)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018 May 26 [PMID: 29802792] (Human)
• Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor-kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017 Oct 31 [PMID: 29375683] (WB, Human)
• Cuffe H, Liu M, Key CC et al. Targeted Deletion of Adipocyte Abca1 (ATP-Binding Cassette Transporter A1) Impairs Diet-Induced Obesity Arterioscler. Thromb. Vasc. Biol. 2018 Jan 18 [PMID: 29348118] (Mouse)
• Canfran-Duque A, Rotllan N, Zhang X et al. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis EMBO Mol Med 2017 Jul 03 [PMID: 28674080] (WB, Mouse)
• de Aguiar Vallim TQ, Lee E, Merriott DJ et al. ABCG1 regulates pulmonary surfactant metabolism in mice and men. J. Lipid Res. Mar 6 2017 [PMID: 28264879]
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017 Feb 16 [PMID: 28205585] (WB, Mouse)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)
• Cheng HY, Gaddis DE, Wu R et al. Loss of ABCG1 influences regulatory T cell differentiation and atherosclerosis. J. Clin. Invest. Sep 1 2016 [PMID: 27482882] (WB, Mouse)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Aryal B, Rotllan N, Araldi E et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. Jul 27 2016 [PMID: 27460411] (WB, Mouse)
• Mostafa A, Hamdy N, Abdel-Rahman S, El-Mesallamy H. Effect of vildagliptin and pravastatin combination on cholesterol efflux in adipocytes. IUBMB Life. [PMID: 27251372] (WB, Mouse)
• Tarling EJ, Edwards PA. Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both ABCG1-L550 and ABCG1-P550. Arterioscler. Thromb. Vasc. Biol. May 26 2016 [PMID: 27230131] (WB, ICC/IF, Mouse, Chinese Hamster, Monkey)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID: 27063951] (WB, Mouse)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)
• Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ. Glucagon-like peptide 1 (GLP-1)-based therapy upregulate LXR-ABCA1/ABCG1 cascade in adipocytes. Biochem. Biophys. Res. Commun. 2015 Nov 18 [PMID: 26603933]
• Matsuo K, Yamamoto S, Wakamatsu T et al. Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate. Toxins (Basel). 2015 Aug 20 [PMID: 26287243] (WB, Human)
  
  Details:
  ABCG1 antibody was used at 1:1000 dilution for WB analysis of lysates from THP-1 cells that were exposed to 1 mM indoxyl sulfate for differentiation for 24 hours (Figure 2).
• Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes. 2015 Aug 07 [PMID: 26253613]
• Lee Tzong-Shyuan, Lu Kuo-Yun, Yu Yuan-Bin et al. B Common Receptor Mediates Erythropoietin-Conferred Protection on OxLDL-Induced Lipid Accumulation and Inflammation in Macrophages. Mediators of Inflammation 2015 [PMID: 26101463] (IHC, Mouse)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015 Feb 21 [PMID: 25708389] (WB, Mouse)
• Kuo Cheng-Hsiang, Leon Lorraine, Chung Eun Ji et al. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B Mater Biol Med. 2014 Dec 14 [PMID: 25685357]
• Park SH, Paek JH, Shin D et al. Purple perilla extracts with alpha-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages Int. J. Mol. Med. 2015 Feb 12 [PMID: 25673178] (WB, Mouse)
• Zheng W, Mast N, Saadane A, Pikuleva Ia. Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments. J. Lipid Res. 2014 Oct 07 [PMID: 25293590] (IHC, Mouse)
• Frisdal E, Lay SL, Hooton H et al. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity. Diabetes. 2014 Sep 23 [PMID: 25249572]
• Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014 Sep 12 [PMID: 25217640]
• Horie Takahiro, Nishino Tomohiro, Baba Osamu et al. MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. Sci Rep. 2014 Jun 16 [PMID: 24931346] (Mouse)
• Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 3/5/2014 [PMID: 24637412] (WB, Mouse)
• Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013 Dec 4 [PMID: 24371802] (WB, Human)
• Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013 Nov 6 [PMID: 24223214] (WB, Mouse)
• Nicod N, Parker RS. Vitamin E Secretion by Caco-2 Monolayers to APOA1, but Not to HDL, Is Vitamer Selective. J Nutr. 2013 Oct [PMID: 23946344] (WB, Human)
• Daniil G, Zannis VI, Chroni A. Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux. PLoS One 2013 Jun 27 [PMID: 23826352] (WB, Mouse, Human)
• Zhou MS, Chadipiralla K, Mendez AJ et al. Nicotine potentiates proatherogenic effects of oxLDL by stimulating and upregulating macrophage CD36 signaling. Am J Physiol Heart Circ Physiol. 2013 Aug [PMID: 23748423] (WB, Human)
• Nakaya K, Ayaori M, Uto-Kondo H et al. Overexpression of stearoyl-coenzyme A desaturase 1 in macrophages promotes reverse cholesterol transport. Biochim Biophys Acta 2013 Aug [PMID: 23747827] (WB, Mouse)
• Fang L, Choi SH, Baek JS et al. Control of angiogenesis by AIBP-mediated cholesterol efflux. Nature 2013 Jun 6 [PMID: 23719382] (WB, Human)
• Doddapattar P, Radovic B, Patankar JV et al. Xanthohumol ameliorates atherosclerotic plaque formation, hypercholesterolemia, and hepatic steatosis in ApoE-deficient mice. Mol Nutr Food Res 2013 May 3 [PMID: 23650230] (WB, Mouse)
• Mulay V, Wood P, Manetsch M et al. Inhibition of Mitogen-Activated Protein Kinase Erk1/2 Promotes Protein Degradation of ATP Binding Cassette Transporters A1 and G1 in CHO and HuH7 Cells. PLoS One 2013 Apr 25 [PMID: 23634230] (WB, Hamster, Human)
• de Beer MC, Wroblewski JM, Noffsinger VP et al. The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A. J Lipids 2013 [PMID: 23431457] (WB, Mouse)
• Huang CX, Zhang YL, Wang Jf et al. MCP-1 impacts RCT by repressing ABCA1, ABCG1, and SR-BI through PI3K/Akt posttranslational regulation in HepG2 cells. J Lipid Res 2013 May [PMID: 23402987]
• Gu HM, Li G, Gao X, Berthiaume LG, Zhang DW. Characterization of palmitoylation of ATP binding cassette transporter G1: effect on protein trafficking and function. Biochim Biophys Acta 2013 Feb 3 [PMID: 23388354]
• El Roz A, Bard JM, Huvelin JM, Nazih H. The anti-proliferative and pro-apoptotic effects of the trans9,trans11 conjugated linoleic acid isomer on MCF-7 breast cancer cells are associated with LXR activation. Prostaglandins Leukot Essent Fatty Acids 2013 Jan 31 [PMID: 23375583] (WB, Human)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013 Feb 19 [PMID: 23334894] (WB, Mouse)
• Horie T, Baba O, Kuwabara Y et al. MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice. J Am Heart Assoc 2012 [PMID: 23316322] (WB, Mouse)
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation Biochem Pharmacol 2013 Jan 9 [PMID: 23313547] (WB, Human)
• Munch G, Bultmann A, Li Z et al. Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits. Heart Int 2012 Jun 5 [PMID: 23185679] (ICC/IF, Rabbit)
• Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013 Jan [PMID: 23117655] (WB, Mouse)
• L Marko, G Paragh, P Ugocsai et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light Biology 2012 (WB, IHC, Human)
• Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human)
• Shen C, Jiang J, Huang C, Zhu W. Gypenoside LVI attenuates foam cell formation by promoting cholesterol export and inhibiting inflammation response Journal of Functional Foods Nov 1 2018 (WB, Mouse)
• Chen J, Zhang X, Kusumo H et al. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis Biochim Biophys Acta 2012 Sep 23 [PMID: 23010475] (WB, Rat)
• Marko L, Paragh G, Ugocsai P et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light J Photochem Photobiol B 2012 Nov 5 [PMID: 22982209] (WB, IHC, Human)
• Gelissen IC, Sharpe LJ, Sandoval C et al. Protein kinase A modulates the activity of a major human isoform of ABCG1 J Lipid Res 2012 Oct [PMID: 22872754]
• Wang SH, Yuan SG, Peng DQ, Zhao SP. HDL and ApoA-I Inhibit Antigen Presentation-mediated T Cell Activation by Disrupting Lipid Rafts in Antigen Presenting Cells Atherosclerosis 2012 Jul 27 [PMID: 22862966] (WB, Mouse)
• Iizuka M, Ayaori M, Uto-Kondo H et al. Astaxanthin enhances ATP-binding cassette transporter A1/G1 expressions and cholesterol efflux from macrophages J Nutr Sci Vitaminol 2012 [PMID: 22790567] (WB, Mouse, Human)
• Olivier M, Tanck MW, Out R et al. Human ATP-binding cassette G1 controls macrophage lipoprotein lipase bioavailability and promotes foam cell formation. Arterioscler Thromb Vasc Biol 2012 Sep [PMID: 22772754]
• El Roz A, Bard JM, Huvelin JM, Nazih H. LXR Agonists and ABCG1-dependent Cholesterol Efflux in MCF-7 Breast Cancer Cells: Relation to Proliferation and Apoptosis Anticancer Res 2012 Jul [PMID: 22753765]
• Yasuyo Okada, Kiyomi Ueyama, Jyun-ichi Nishikawa, Masanori Semma, Atsushi Ichikawa. Effect of 6-O-alpha-maltosyl-beta cyclodextrin and its cholesterol inclusion complex on cellular cholesterol levels and ABCA1 and ABCG1 expression in mouse mastocytoma P-815 cells,. Carbohydrate Research, 10.1016/j.carres.2012.04.019. 2012 [PMID: 22677519] (WB, Mouse)
• Miroshnikova VV, Demina EP, Mayorov NV. The expression of ABCG1 transporter gene in peripheral blood mononuclear cells of patients with atherosclerosis. Cell and Tissue Biology. 2014
• Kuo CH, Leon L, Chung EJ, Huang RT. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. Journal of Materials Chemistry B. 2014 Sep 05
• Reboulleau A, Robert V, Vedie B et al. Involvement of cholesterol efflux pathway in the control of cardiomyocytes cholesterol homeostasis. Journal of Molecular and Cellular Cardiology. 2012 June 3. [PMID: 22668787] (WB, Rat)
• Zhao JF, Ching LC, Huang YC, Chen CY, Chiang AN, Kou YR, Shyue SK, Lee TS. Molecular mechanism of curcumin on the suppression of cholesterol accumulation in macrophage foam cells and atherosclerosis. Mol Nutr Food Res;56(5):691-701. 2012 May. [PMID: 22648616] (WB, Mouse)
• Tang S-L, Chen W-J, Yin K, et el. PAPP-A Negatively Regulates ABCA1, ABCG1 and SR-B1 Expression by inhibiting LXR alpha through the IGF-I-mediated signaling pathway Atherosclerosis 2012 Jun [PMID: 22503545] (WB, Human)
• Cui W, SunY,Wang Z et al. Liver X receptor activation attenuates inflammatory response and protects cholinergic neurons in APP/PS1 transgenic mice Neuroscience Mar 5 2012 [PMID: 22425753] (WB, Mouse)
• Meurs I, Calpe-Berdiel L, Habets KLL, Zhao Y, Korporaal SJA et al. Effects of Deletion of Macrophage ABCA7 on Lipid Metabolism and the Development of Atherosclerosis in the Presence and Absence of ABCA1 PLoS ONE 2012 [PMID: 22403608] (WB, Mouse)
• Morbitzer D, Namgaladze D, Brune B. Phospholipase A(2)-Modified Low-Density Lipoprotein Activates Liver X Receptor in Human Macrophages Cell Biochem Biophys 2012 Feb 26 [PMID: 22367754] (WB, Human)
• Ayaori M, Yakushiji E, Ogura M, Nakaya K, Hisada T, Uto-Kondo H, Takiguchi S, Terao Y, Sasaki M, Komatsu T, Iizuka M, Yogo M, Uehara Y, Kagechika H, Nakanishi T, Ikewaki K. Retinoic acid receptor agonists regulate expression of ATP-binding cassette transporter G1 in macrophages. Biochim Biophys Acta;1821(4):561-572. 2012 Feb 14. [PMID: 22353356]
• Morales CR, Ni X, Smith CE et al. ABCA17 mediates sterol efflux from mouse spermatozoa plasma membranes. Histol Histopathol 27(3). 2012 Mar. [PMID: 22237709] (In vitro, Mouse)
• Ku CS, Park Y, Coleman SL, Lee J. Unsaturated fatty acids repress expression of ATP binding cassette transporter A1 and G1 in RAW 264. 7 macrophages. The Journal of Nutritional Biochemistry. 2011 Dec 29. [PMID: 22209005]
• Ji A, Wroblewski JM, Cai L et al. Nascent HDL formation in hepatocytes and role of ABCA1, ABCG1, and SR-BI. J Lipid Res 2012 Mar [PMID: 22190590] (WB)
• Edgel KA, McMillen TS, Wei H et al. Obesity and weight loss result in increased adipose tissue ABCG1 expression in db/db mice. Biochim Biophys Acta. 2011 Dec 10. [PMID: 22179025]
• Tallada Iborra R, Machado-Lima A, Castilho G et al. Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1. Lipids in Health and Disease;10(1):172. 2011 Sep 29. [PMID: 21957962]
• Nagashima M, Watanabe T, Terasaki M et al. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice Diabetologia 2011 Oct [PMID: 21786155] (WB, Mouse)
• Corcoran MP, Lichtenstein AH, Meydani M et al. The effect of 17{beta}-estradiol on cholesterol content in human macrophages is influenced by the lipoprotein milieu. J Mol Endocrinol. 2011 Jun 6. [PMID: 21646386] (WB, Human)
• Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther;338(3):870-8. 2011 Sep. [PMID: 21628419]
• Ji A, Meyer JM, Cai L et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011 Apr 8. [PMID: 21481393]
• Wong BXW, Kyle RA, Croft KD et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids;46(4):371-80. 2011 Apr. [PMID: 21286835]
• Law RH, Lukoyanova N, Voskoboinik I et al. The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 2010 Nov 18 [PMID: 21037563] (WB)
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010 Aug [PMID: 20629037]
• Aye IL, Waddell BJ, Mark PJ, Keelan JA. Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity. Biochim Biophys Acta 2010 Sep [PMID: 20570635] (Human)
• Uto-Kondo H, Ayaori M, Ogura M et al. Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. Circ Res;106(4):779-87. 2010 Mar 5. [PMID: 20075335] (WB, Mouse)
• Nagelin MH, Srinivasan S, Nadler JL et al. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem;284(45):31303-14. 2009 Nov 6. [PMID: 19713213]
• Lee TS, Lu KY, Yu YB et al. beta Common Receptor Mediates Erythropoietin-Conferred Protection on OxLDL-Induced Lipid Accumulation and Inflammation in Macrophages Mediators of Inflammation. 2015 Jan 01 (WB, Mouse)
• Machado J. T. N-acetylcysteine reduces endoplasmic reticulum stress and affects selectively cholesterol efflux from macrophages mediated by ABCA-1 and ABCG-1 in chronic kidney disease. Thesis. 2014 Sep 01 (WB, Rat)
• Welch C, Santra MK, El-Assaad W et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472CAN-0009-0128. [PMID: 19706769]
• Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res :CIRCRESAHA.109.193870. 2009 [PMID: 19644050]
• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Endocrinol Metab 2009 Aug [PMID: 19625677] (WB, Human)
• Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009 [PMID: 19590047]
• Ronis MJ et al. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr;139(8):1431-8. 2009 Aug. [PMID: 19515742]
• Ouvrier A, Cadet R, Vernet P et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res :M800657-JLR800200. 2009 [PMID: 19395734] (WB, IHC-P, Mouse)
• Bujold K et al. CD36-mediated cholesterol efflux is associated with PPARgamma activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res;83(3):457-64. 2009 Aug 1. [PMID: 19377069]
• Rohrer L, Ohnsorg PM, Lehner M et al. High-density lipoprotein transport through aortic endothelial cells involves scavenger receptor BI and ATP-binding cassette transporter G1. Circ Res 2009 May 22 [PMID: 19372466]
• Sirek AS, Liu L, Naples M et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology;150(8):3483-3492. 2009 [PMID: 19359385]
• Delvecchio CJ, Bilan P, Nair P et al. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol;295(5):L949-57. 2008 Nov. [PMID: 18820007]
• Nakanishi S, Vikstedt R, Soderlund S et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res;50(2):183-92. 2009 Feb. [PMID: 18787236]
• Mauldin JP et al. Reduced expression of ATP-binding cassette transporter G1 increases cholesterol accumulation in macrophages of patients with type 2 diabetes mellitus. Circulation;117(21):2785-92. 2008 May 27. [PMID: 18490524]
• Lorenzi I, von Eckardstein A, Radosavljevic S, Rohrer L Lipidation of apolipoprotein A-I by ATP-binding cassette transporter (ABC) A1 generates an interaction partner for ABCG1 but not for scavenger receptor BI. Biochim Biophys Acta 2008 Jun-Jul [PMID: 18485926]
• Ouimet M, Wang M-D, Cadotte N et al. Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Arterioscler Thromb Vasc Biol;28(6):1144-50. 2008 Jun. [PMID: 18369155]
• Wojcik AJ et al. A critical role for ABCG1 in macrophage inflammation and lung homeostasis. J Immunol;180(6):4273-82. 2008 Mar 15. [PMID: 18322240]
• Burgess, BL et al. ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J Lipid Res;49(6):1254-67. 2008 Jun. [PMID: 18314463]
• Wahrle SE, Jiang H, Parsadanian M et al. Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. J Clin Invest 2008 Feb [PMID: 18202749] (WB, Mouse)
• Hu, W et al. Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J Lipid Res;49(2):386-93. 2008 Feb. [PMID: 18033752]
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Reviews for ABCG1 Antibody (NB400-132) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Angeliki Chroni WB Human 10/08/2014

Summary

• Application: Western Blot
• Sample Tested: ABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
• Species: Human

reviewed by: Anonymous WB Mouse 08/26/2013

Summary

• Application: Western Blot
• Species: Mouse
• Special Applications: Excellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
• Pub Med ID: 23826352

Bioinformatics

• Gene Symbol: ABCG1