ABCG1 Antibody - BSA Free

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: ABCG1 Antibody - BSA Free [NB400-132] - Staining of human lung, alveolar macrophages.

Flow (Intracellular): ABCG1 Antibody - BSA Free [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody.

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Cells were pretreated with 5 uM F12511 or 5 uM MG132 for 30 min and then exposed to 5 or 50 uM 24S-OHC for 6 h. Whole-cell lysates were immunoblotted with antibodies specific for ABCG1 or beta-actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31285856/) licensed under a CC-BY license.

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated macrophages, Lane 3: T0901713 treated macrophages, Lane 4: T091713 treated macrophages.

Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected subcutaneously with PBS or 0.8 ug/g LPS as indicated, followed 4 hr later by intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages. ABCG1 in liver lysates (40 ug protein) was identified by immunoblotting and quantified by densitometry. Results from each of the 3 experiments (n = 4-5 per group per experiment) were expressed relative to PBS-injected WT mice after normalization to beta-actin. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/283486/), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, ChHa, Ha, Pm, Rb
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ABCG1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting reported in scientific literature (PMID 28264879)
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23185679)
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation reported in scientific literature (PMID 30235209)
  • In vitro assay
  • In vivo assay reported in scientific literature
  • Western Blot 1:500
• Theoretical MW: 75.59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  THP-1 TO901317 Treated / Untreated Cell Lysate

• Control Peptide:

  ABCG1 Protein (NB400-132PEP)

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID: 27230131).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCG1 Antibody - BSA Free

• ABC transporter 8
• ABC8
• ABCG1
• ATP-binding cassette sub-family G member 1
• ATP-binding cassette transporter 8
• ATP-binding cassette transporter member 1 of subfamily G
• ATP-binding cassette, sub-family G (WHITE), member 1
• homolog of Drosophila white
• MGC34313
• white protein homolog (ATP-binding cassette transporter 8)
• White protein homolog
• WHITE1
• WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1) is a member of the ABC superfamily of transporters, specifically the ABCG (White) subfamily, that is characterized by their ABC domain organization (1). The ABC domains are around 180 amino acids (aa) in length and contain three conserved domains: Walker A motif (12 aa), Walker B motif (5 aa), and Signature motif (5 aa) (1, 2). Functional ABC transporters are comprised of two ABCs and two transmembrane domains (TMDs), where each TMD contains six TM alpha-helices (1-3). ABCG1 is considered a "half" transporter because it only has one ABC domain and one TMD (1-3). In order to be functional, ABCG1 and other half transporters can form homodimers or heterodimers (1-3). ABCG1 has a theoretical molecular weight of 102 kDa and is highly expressed in cells including macrophages, lymphocytes, and neurons (2). Additionally, ABCG1 is shown to be highly expressed in lung, kidney, brain, and spleen tissue (2). Specifically, ABCG1 is presented on the cell surface and in intracellular compartments of cholesterol-rich macrophages and, consequently, plays a crucial role in cholesterol regulation and homeostasis (3-5). ABCG1 is involved in the export, or efflux, of cholesterol and phospholipids from macrophages to high density lipoproteins (HDL) for eventual excretion via the liver.

A variety of cardiovascular and cardiometabolic diseases are associated with ABCG1 dysfunction (5-7). Macrophages can become cholesterol-containing foam cells that are generated by the uptake of low-density lipoproteins (LDL), cholesterol esterification, and compromised cholesterol efflux machinery in transporters like ABCG1 and ABCA1 (2, 5, 6, 7). Foam cells are associated with the chronic, inflammatory disease atherosclerosis which is characterized by arterial buildup of plaques that can ultimately lead to cardiovascular disease (5, 6, 7). Additionally, ABCG1 has a critical role in cardiometabolic disorders. Studies have found that diabetic mice have decreased ABCG1 expression (8). Furthermore, loss of ABCG1 in mouse pancreatic beta cells ultimately leads to impaired insulin secretion, suggesting that inhibition or modulation of ABCG1 may contribute to development of diabetes and obesity (8). Finally, other related ATP-binding cassette transporter family members, such as ABCA1 and ABCG5/8, have been associated with genetically-inherited syndromes like Tangier disease, characterized by reduced levels of HDL in the blood, and Sitosterolemia, characterized by elevated plant sterol lipid accumulation in blood and tissues (7). .

Alternate names for ABCG1 includes ABC transporter 8 (ABC8), ATP-binding cassette transporter, anti-, sub-family G (WHITE), homolog of Drosophila white, and MGC34313. .

References

1. Tarling E. J. (2013). Expanding roles of ABCG1 and sterol transport. Current opinion in lipidology. https://doi.org/10.1097/MOL.0b013e32835da122.

2. Tarr, P. T., Tarling, E. J., Bojanic, D. D., Edwards, P. A., & Baldan, A. (2009). Emerging new paradigms for ABCG transporters. Biochimica et biophysica acta.https://doi.org/10.1016/j.bbalip.2009.01.007.

3. Tarling, E. J., & Edwards, P. A. (2011). ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1113021108.

4. Phillips M. C. (2014). Molecular mechanisms of cellular cholesterol efflux. The Journal of biological chemistry, 289(35), 24020-24029. https://doi.org/10.1074/jbc.R114.583658.

5. Ouimet, M., Barrett, T. J., & Fisher, E. A. (2019). HDL and Reverse Cholesterol Transport. Circulation research. https://doi.org/10.1161/CIRCRESAHA.119.312617.

6. Yu, X. H., Fu, Y. C., Zhang, D. W., Yin, K., & Tang, C. K. (2013). Foam cells in atherosclerosis. Clinica chimica acta; international journal of clinical chemistry. https://doi.org/10.1016/j.cca.2013.06.006

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG1 Antibody (NB400-132)(175)

Publications using NB400-132

• Yalcinkaya M, Fotakis P, Liu W et al. Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1b secretion Cardiovascular research 2022-12-20 [PMID: 36537208] (WB, Mouse)
• Bruggmann N Untersuchungen zum ABCA1-/ABCG1-vermittelten Cholesterin-Efflux in humanen Kontroll-und Tangierfibroblasten Thesis 2021-01-01 (WB, Human, Primate)
• Machado J. T. N-acetylcysteine reduces endoplasmic reticulum stress and affects selectively cholesterol efflux from macrophages mediated by ABCA-1 and ABCG-1 in chronic kidney disease. Thesis. 2014-09-01 (WB, Rat)
• Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (ICC/IF, IHC-Fr)
• Gro beta E, Hilger RA, Schümann FL et al. SAM-Competitive EZH2-Inhibitors Induce Platinum Resistance by EZH2-Independent Induction of ABC-Transporters Cancers 2023-06-03 [PMID: 37297005] (WB, Human)
• Wang P, Li M, Gao T et al. Vascular Electrical Stimulation with Wireless, Battery-Free, and Fully Implantable Features Reduces Atherosclerotic Plaque Formation Through Sirt1-Mediated Autophagy Small (Weinheim an der Bergstrasse, Germany) 2023-06-02 [PMID: 37267941] (WB, Mouse)
• Cao D, Khan Z, Li X et al. Macrophage angiotensin-converting enzyme (ACE) reduces atherosclerosis by increasing PPAR? and fundamentally changing lipid metabolism Cardiovascular research 2023-05-24 [PMID: 37225143] (WB, Mouse)
• Prasad R, Adu-Agyeiwaah Y, Floyd JL et al. Sustained ACE2 Expression by Probiotic Improves Integrity of Intestinal Lymphatics and Retinopathy in Type 1 Diabetic Model Journal of clinical medicine 2023-02-23 [PMID: 36902558] (IHC-P, Mouse)
• Cassim Bawa FN, Gopoju R, Xu Y et al. Retinoic Acid Receptor Alpha (RARa) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice Cells 2022-10-11 [PMID: 36291054] (WB, Mouse)
• Xu H, Zheng LX, Chen XS et al. Brain-specific loss of Abcg1 disturbs cholesterol metabolism and aggravates pyroptosis and neurological deficits after traumatic brain injury Brain Pathology 2023-05-01 [PMID: 36271611] (ICC/IF, IHC)
• Cai Y, Wang Z, Li L et al. Neuropeptide Y regulates cholesterol uptake and efflux in macrophages and promotes foam cell formation Journal of cellular and molecular medicine 2022-09-29 [PMID: 36172879] (WB, Human)
• Man B, Hu C, Yang G et al. Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPAR alpha in ApoE-/- mice Biochemical and Biophysical Research Communications 2022-07-01 [PMID: 35933927] (WB, Mouse)
  
  Details:
  Figure 3E
• Basu R, Qian Y, Mathes S et al. Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo Frontiers in oncology 2022-07-05 [PMID: 35865483] (WB, Mouse)
• Xi B, Luo FZ, He B et al. High nuclear ABCG1 expression is a poor predictor for hepatocellular carcinoma patient survival Hepatobiliary & pancreatic diseases international : HBPD INT 2022-06-01 [PMID: 35778316] (IF/IHC, Human)
• Vaisar T, Wang S, Omer M et al. 10,12-conjugated linoleic acid supplementation improves HDL composition and function in mice Journal of lipid research 2022-06-14 [PMID: 35714730] (WB, Mouse)
• Esobi I, Olanrewaju O, Echesabal-Chen J, Stamatikos A. Utilizing the LoxP-Stop-LoxP System to Control Transgenic ABC-Transporter Expression In Vitro Biomolecules 2022-05-08 [PMID: 35625607]
• Zhang X, Rotllan N, Canfran-Duque A et al. Targeted Suppression of miRNA-33 Using pHLIP Improves Atherosclerosis Regression Circulation research 2022-05-09 [PMID: 35534923] (WB, Mouse)
• Yildirim Z, Baboo S, Hamid SM et al. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression EMBO molecular medicine 2022-02-22 [PMID: 35191199] (WB, Mouse)
• Balamurugan K, Medishetti R, Kotha J et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways iScience 2022-02-01 [PMID: 35141506] (WB, Human, Mouse)
• Shinohata R, Shibakura M, Arao Y et al. A high-fat/high-cholesterol diet, but not high-cholesterol alone, increases free cholesterol and apoE-rich HDL serum levels in rats and upregulates hepatic ABCA1 expression Biochimie 2022-01-24 [PMID: 35085709] (WB, Rat)
• Hueso M, GriNAn R, Mallen A et al. MiR-125b downregulates macrophage scavenger receptor type B1 and reverse cholesterol transport Biomedicine & Pharmacotherapy 2022-02-01 [PMID: 35062066] (WB, Human, Mouse)
• Kane J, Jansen M, Hendrix S et al. Anti-Galectin-2 antibody treatment reduces atherosclerotic plaque size and alters macrophage polarity Thrombosis and haemostasis 2021-12-01 [PMID: 34852377] (IF/IHC, Mouse)
• Zhang X, McDonald JG, Aryal B Et al. Desmosterol suppresses macrophage inflammasome activation and protects against vascular inflammation and atherosclerosis Proceedings of the National Academy of Sciences of the United States of America 2021-11-23 [PMID: 34782454] (WB, Mouse)
• Kang CM, Li WK, Yu KW Et al. Long non coding RNA AL355711 promotes smooth muscle cell migration through the ABCG1/MMP3 pathway International journal of molecular medicine 2021-12-01 [PMID: 34608503] (WB)
• Sun Y, Wang J, Long T Et al. Molecular basis of cholesterol efflux via ABCG subfamily transporters Proceedings of the National Academy of Sciences of the United States of America 2021-08-24 [PMID: 34404721] (WB, Human)
• Wang H, Zhang P, Chen X Et Al. Activin a inhibits foam cell formation and up-regulates ABCA1 and ABCG1 expression through Alk4-Smad signaling pathway in RAW 264.7 macrophages Steroids 2021-07-06 [PMID: 34237315]
• Ram�rez CM, Torrecilla-Parra M, Pardo-Marqu�s V et al. Crosstalk Between LXR and Caveolin-1 Signaling Supports Cholesterol Efflux and Anti-Inflammatory�Pathways�in Macrophages Front Endocrinol (Lausanne) 2021-05-27 [PMID: 34122329]
• Zhang S, Li Z, Zhang Y et al. Ketone Body 3 Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a Mediated Calcium Influx Advanced Science 2021-03-01 [PMID: 33977048] (WB, Mouse)
• Tsui PF, Chern CY, Lien CF et al. An octimibate derivative, Oxa17, enhances cholesterol efflux and exerts anti-inflammatory and atheroprotective effects in experimental atherosclerosis Biochemical pharmacology 2021-04-23 [PMID: 33895158]
• Esobi IC, Barksdale C, Heard-Tate C et al. MOVAS Cells: A Versatile Cell Line for Studying Vascular Smooth Muscle Cell Cholesterol Metabolism Lipids 2021-04-21 [PMID: 33881166]
• Liu W, Liu J, Xing S et al. The benzoate plant metabolite ethyl gallate prevents cellular- and vascular-lipid accumulation in experimental models of atherosclerosis Biochemical and biophysical research communications 2021-06-04 [PMID: 33839416]
• Sun R, Fang P, Jiang J et al. Insulin Rescued MCP-1-Suppressed Cholesterol Efflux to Large HDL2 Particles via ABCA1, ABCG1, SR-BI and PI3K/Akt Activation in Adipocytes Cardiovascular drugs and therapy 2021-03-19 [PMID: 33740174]
• Price NL, Zhang X, Fernández-Tussy P, et al. Loss of hepatic miR-33 improves metabolic homeostasis and liver function without altering body weight or atherosclerosis Proceedings of the National Academy of Sciences of the United States of America 2021-02-02 [PMID: 33495342] (WB, Mouse)
• Hou X, Zhang C, Wang L, Wang K Natural Piperine Improves Lipid Metabolic Profile of High-Fat Diet-Fed Mice by Upregulating SR-B1 and ABCG8 Transporters Journal of natural products 2021-01-25 [PMID: 33492139]
• Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology 2020-12-14 [PMID: 33381046] (WB, Mouse)
• Ren Q, Xie X, Tang Y et al. Methyl tertiary-butyl ether inhibits THP-1 macrophage cholesterol efflux in vitro and accelerates atherosclerosis in ApoE-deficient mice in vivo J Environ Sci (China) 2020-12-18 [PMID: 33334519]
• Qian Y, Basu R, Mathes SC et al. Growth Hormone Upregulates Mediators of Melanoma Drug Efflux and Epithelial-to-Mesenchymal Transition In Vitro and In Vivo Cancers 2020-12-04 [PMID: 33291663] (WB, Human, Mouse)
• Alsina-Sanchis, E, MUlfarth, R Et al. Intraperitoneal Oil Application Causes Local Inflammation with Depletion of Resident Peritoneal Macrophages. Mol Cancer Res 2021-02-01 [PMID: 33139505] (WB, Human)
• Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics 2020-08-13 [PMID: 32929345] (WB, Mouse)
• Ohkawa, R, Low, H Et al. Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood. J Lipid Res 2020-12-01 [PMID: 32907987] (WB, Mouse)
• A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells Sci Rep, 2020-08-19;10(1):13988. 2020-08-19 [PMID: 32814794] (WB, Human)
• Barilla S, Liang N, Mileti E et al. Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling through upregulation of ATP binding cassette subfamily G member 1 Mol Metab 2020-08-13 [PMID: 32798719] (WB, Human)
• Donadon M, Torzilli G, Cortese N et al. Macrophage morphology correlates with single-cell diversity and prognosis in colorectal liver metastasis J. Exp. Med. 2020-11-02 [PMID: 32785653] (FLOW, Human)
• Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research 2020-07-01 [PMID: 32621955] (WB, Mouse)
• Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. 2020-06-30 [PMID: 32602556] (WB, Rat)
• Chen LW, Tsai MC, Chern CY et al. A chalcone derivative, 1m-6, exhibits atheroprotective effects by increasing cholesterol efflux and reducing inflammation-induced endothelial dysfunction Br. J. Pharmacol. 2020-06-24 [PMID: 32579243]
• Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. 2020-05-08 [PMID: 32437644] (WB, Human)
• Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020-05-21 [PMID: 32437392] (WB, Human)
• Shen CY, Wang TX, Jiang JG et al. Bergaptol from blossoms of Citrus aurantium L. var. amara Engl inhibits LPS-induced inflammatory responses and ox-LDL-induced lipid deposition Food Funct 2020-05-20 [PMID: 32432251]
• Salonurmi T, Nabil H, Ronkainen J et Al. 4 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. 2020-03-27 [PMID: 32292343] (WB, Rat)
• Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. 2020-02-28 [PMID: 32173266] (WB, Mouse)
• Guo Y MicroRNA-205-5p Promotes Unstable Atherosclerotic Plaque Formation In Vivo Cardiovasc Drugs Ther 2020-02-08 [PMID: 32034643] (Mouse)
• Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. 2019-11-14 [PMID: 31771811] (WB, Mouse)
• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019-09-12 [PMID: 31604677]
• Urano Y, Ho Vo DK, Hirofumi A, Noguchi N 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death Cell Death Discov 2019-07-05 [PMID: 31285856] (WB, Human)
• Wu A, Grela E, Woojtowicz K, et al. ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells Cell. Mol. Life Sci. 2019-05-27 [PMID: 31134303] (WB, Chinese Hamster)
• Pasca AM, Park JY, Shin HW et al. Cyclodextrin Ameliorates the Progression of Atherosclerosis via Increasing High-Density Lipoprotein Cholesterol Plasma Levels and Anti-inflammatory Effects in Rabbits J. Cardiovasc. Pharmacol. 2019-05-01 [PMID: 30855405]
• Sedgeman LR, Beysen C, Ramirez Solano MA et al. Beta cell secretion of miR-375 to HDL is inversely associated with insulin secretion Sci Rep 2019-03-07 [PMID: 30846744] (WB, Mouse)
• Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019-01-01 [PMID: 30389579] (Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Harris MT, Hussain SS, Inouye CM et al. Reinterpretation of the localization of the ATP binding cassette transporter ABCG1 in insulin-secreting cells and insights regarding its trafficking and function PLoS One. 2018-09-19 [PMID: 30235209] (IP, Rat)
• Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International 2018-07-12 [PMID: 30105261] (WB, Rabbit)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. 2018-07-23 [PMID: 30038271] (WB, Human)
• Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018-06-15 [PMID: 29906247] (Mouse)
• Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim Biophys Acta Mol Cell Biol Lipids. [PMID: 29890224] (WB, Mouse)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018-05-26 [PMID: 29802792] (Human)
• Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor‑kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017-10-31 [PMID: 29375683] (WB, Human)
• Cuffe H, Liu M, Key CC et al. Targeted Deletion of Adipocyte Abca1 (ATP-Binding Cassette Transporter A1) Impairs Diet-Induced Obesity Arterioscler. Thromb. Vasc. Biol. 2018-01-18 [PMID: 29348118] (Mouse)
• de Aguiar Vallim TQ, Lee E, Merriott DJ et al. ABCG1 regulates pulmonary surfactant metabolism in mice and men. J. Lipid Res. 2017-03-06 [PMID: 28264879]
• Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 (IHC-Fr, Mouse)
  
  Details:
  1:100 dilution
• Canfran-Duque A, Rotllan N, Zhang X et al. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis EMBO Mol Med 2017-07-03 [PMID: 28674080] (WB, Mouse)
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017-02-16 [PMID: 28205585] (WB, Mouse)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. 2016-11-24 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. 2016-11-15 [PMID: 27862930] (WB, Human)
• Cheng HY, Gaddis DE, Wu R et al. Loss of ABCG1 influences regulatory T cell differentiation and atherosclerosis. J. Clin. Invest. 2016-09-01 [PMID: 27482882] (WB, Mouse)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Aryal B, Rotllan N, Araldi E et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016-07-27 [PMID: 27460411] (WB, Mouse)
• Miroshnikova VV, Panteleeva AA, Bazhenova EA et al. [Regulation of ABCA1 and ABCG1 gene expression in the intraabdominal adipose tissue] Biomeditsinskaya Khimiya 2016-07-16 [PMID: 27420620]
• Mostafa A, Hamdy N, Abdel-Rahman S, El-Mesallamy H. Effect of vildagliptin and pravastatin combination on cholesterol efflux in adipocytes. IUBMB Life. [PMID: 27251372] (WB, Mouse)
• Tarling EJ, Edwards PA. Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both ABCG1-L550 and ABCG1-P550. Arterioscler. Thromb. Vasc. Biol. 2016-05-26 [PMID: 27230131] (ICC/IF, WB, Monkey, Mouse, Chinese Hamster)
• Westerterp M, Tsuchiya K et al. Deficiency of ATP-Binding Cassette Transporters A1 and G1 in Endothelial Cells Accelerates Atherosclerosis in Mice. Arterioscler Thromb Vasc Biol 2016-01-07 [PMID: 27199450] (WB, Mouse)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016-04-22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016-04-10 [PMID: 27063951] (WB, Mouse)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep 2016-01-29 [PMID: 26821916] (WB, Mouse)
• Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ. Glucagon-like peptide 1 (GLP-1)-based therapy upregulate LXR-ABCA1/ABCG1 cascade in adipocytes. Biochem. Biophys. Res. Commun. 2015-11-18 [PMID: 26603933]
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  Details:
  ABCG1 antibody was used at 1:1000 dilution for WB analysis of lysates from THP-1 cells that were exposed to 1 mM indoxyl sulfate for differentiation for 24 hours (Figure 2).
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• Yasuyo Okada, Kiyomi Ueyama, Jyun-ichi Nishikawa, Masanori Semma, Atsushi Ichikawa. Effect of 6-O-alpha-maltosyl-beta cyclodextrin and its cholesterol inclusion complex on cellular cholesterol levels and ABCA1 and ABCG1 expression in mouse mastocytoma P-815 cells, . Carbohydrate Research, 10.1016/j.carres.2012.04.019. 2012-01-01 [PMID: 22677519] (WB, Mouse)
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• Zhao JF, Ching LC, Huang YC, Chen CY, Chiang AN, Kou YR, Shyue SK, Lee TS. Molecular mechanism of curcumin on the suppression of cholesterol accumulation in macrophage foam cells and atherosclerosis. Mol Nutr Food Res;56(5):691-701. 2012-05-01 [PMID: 22648616] (WB, Mouse)
• Tang S-L, Chen W-J, Yin K, et el. PAPP-A Negatively Regulates ABCA1, ABCG1 and SR-B1 Expression by inhibiting LXR alpha through the IGF-I-mediated signaling pathway Atherosclerosis 2012-06-01 [PMID: 22503545] (WB, Human)
• Cui W, SunY ,Wang Z et al. Liver X receptor activation attenuates inflammatory response and protects cholinergic neurons in APP/PS1 transgenic mice Neuroscience 2012-03-05 [PMID: 22425753] (WB, Mouse)
• Meurs I, Calpe-Berdiel L, Habets KLL, Zhao Y, Korporaal SJA et al. Effects of Deletion of Macrophage ABCA7 on Lipid Metabolism and the Development of Atherosclerosis in the Presence and Absence of ABCA1 PLoS ONE 2012-01-01 [PMID: 22403608] (WB, Mouse)
• Morbitzer D, Namgaladze D, Brune B. Phospholipase A(2)-Modified Low-Density Lipoprotein Activates Liver X Receptor in Human Macrophages Cell Biochem Biophys 2012-02-26 [PMID: 22367754] (WB, Human)
• Ayaori M, Yakushiji E, Ogura M, Nakaya K, Hisada T, Uto-Kondo H, Takiguchi S, Terao Y, Sasaki M, Komatsu T, Iizuka M, Yogo M, Uehara Y, Kagechika H, Nakanishi T, Ikewaki K. Retinoic acid receptor agonists regulate expression of ATP-binding cassette transporter G1 in macrophages. Biochim Biophys Acta;1821(4):561-572. 2012-02-14 [PMID: 22353356]
• Morales CR, Ni X, Smith CE et al. ABCA17 mediates sterol efflux from mouse spermatozoa plasma membranes. Histol Histopathol 27(3). 2012-03-01 [PMID: 22237709] (In vitro, Mouse)
• Ku CS, Park Y, Coleman SL, Lee J. Unsaturated fatty acids repress expression of ATP binding cassette transporter A1 and G1 in RAW 264. 7 macrophages. The Journal of Nutritional Biochemistry. 2011-12-29 [PMID: 22209005]
• Ji A, Wroblewski JM, Cai L et al. Nascent HDL formation in hepatocytes and role of ABCA1, ABCG1, and SR-BI. J Lipid Res 2012-03-01 [PMID: 22190590] (WB)
• Edgel KA, McMillen TS, Wei H et al. Obesity and weight loss result in increased adipose tissue ABCG1 expression in db/db mice. Biochim Biophys Acta 2012-03-01 [PMID: 22179025]
• Tallada Iborra R, Machado-Lima A, Castilho G et al. Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1. Lipids in Health and Disease;10(1):172. 2011-09-29 [PMID: 21957962]
• Nagashima M, Watanabe T, Terasaki M et al. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice Diabetologia 2011-10-01 [PMID: 21786155] (WB, Mouse)
• Corcoran MP, Lichtenstein AH, Meydani M et al. The effect of 17{beta}-estradiol on cholesterol content in human macrophages is influenced by the lipoprotein milieu. J Mol Endocrinol. 2011-06-06 [PMID: 21646386] (WB, Human)
• Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther;338(3):870-8. 2011-09-01 [PMID: 21628419]
• Ji A, Meyer JM, Cai L et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011-04-08 [PMID: 21481393]
• Wong BXW, Kyle RA, Croft KD et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids;46(4):371-80. 2011-04-01 [PMID: 21286835]
• Law RH, Lukoyanova N, Voskoboinik I et al. The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 2010-11-18 [PMID: 21037563] (WB)
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010-08-01 [PMID: 20629037]
• Aye IL, Waddell BJ, Mark PJ, Keelan JA. Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity. Biochim Biophys Acta 2010-09-01 [PMID: 20570635] (Human)
• Uto-Kondo H, Ayaori M, Ogura M et al. Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. Circ Res;106(4):779-87. 2010-03-05 [PMID: 20075335] (WB, Mouse)
• Larrede S, Quinn C M et al. Stimulation of cholesterol efflux by LXR agonists in cholesterol-loaded human macrophages is ABCA1-dependent but ABCG1-independent. Arterioscler Thromb Vasc Biol 2009-01-11 [PMID: 19729607] (WB, Human)
• Nagelin MH, Srinivasan S, Nadler JL et al. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem;284(45):31303-14. 2009-11-06 [PMID: 19713213]
• Welch C, Santra MK, El-Assaad W et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472CAN-0009-0128. [PMID: 19706769]
• Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res;105(5):500-510. 2009-01-01 [PMID: 19644050]
• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Cell Physiol 2009-08-01 [PMID: 19625677] (WB, Human)
• Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009-01-01 [PMID: 19590047]
• Ronis MJ et al. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr;139(8):1431-8. 2009-08-01 [PMID: 19515742]
• Ouvrier A, Cadet R, Vernet P et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res :M800657-JLR800200. 2009-01-01 [PMID: 19395734] (IHC-P, WB, Mouse)
• Bujold K, Rhainds D, Jossart C et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res :cvp118. 2009-01-01 [PMID: 19377069]
• Rohrer L, Ohnsorg PM, Lehner M et al. High-density lipoprotein transport through aortic endothelial cells involves scavenger receptor BI and ATP-binding cassette transporter G1. Circ Res 2009-05-22 [PMID: 19372466]
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Reviews for ABCG1 Antibody (NB400-132) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Angeliki Chroni WB Human 10/08/2014

Summary

• Application: Western Blot
• Sample Tested: ABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
• Species: Human

reviewed by: Verified Customer WB Mouse 08/26/2013

Summary

• Application: Western Blot
• Species: Mouse

FAQs for ABCG1 Antibody (NB400-132). (Showing 1 - 1 of 1 FAQs).

1. What secondary antibodies would be recommended for immunofluorescent or Western blot assays when using NB400-132 as the primary antibody?
   • Under the "Support and Research" tab on the NB400-132 product page on the Novus website, there are links to Anti-Rabbit labeled antibodies and isotype controls that may be useful for your experiments.

Bioinformatics

• Gene Symbol: ABCG1