ABCG1 Antibody

Images

 
Biological Strategies: Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated ...read more
Biological Strategies: Flow (Intracellular): ABCG1 Antibody [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular ...read more
Biological Strategies: Western Blot: ABCG1 Antibody [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for ...read more
Immunohistochemistry-Paraffin: ABCG1 Antibody [NB400-132] - Staining of human lung, alveolar macrophages.
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.
Western Blot: ABCG1 Antibody [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.
Western Blot: ABCG1 Antibody [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected subcutaneously ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Ha, Pm, RbSpecies Glossary
Applications WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, In vitro, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

ABCG1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation
  • In vitro assay
  • Flow (Intracellular)
Application Notes
Preliminary results suggest that this antibody does not work in IP. In vivo assay was reported in scientific literature. Use in ICC/IF reported in scientific literature (PMID 23185679). Use in immunoblotting reported in scientific literature (PMID: 28264879). Use in immunoprecipitation reported in scientific literature (PMID:30235209).
Theoretical MW
75.59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ABCG1 Protein (NB400-132PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB400-132 in the following applications:

Publications
Read Publications using
NB400-132 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID 27230131).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCG1 Antibody

  • ABC transporter 8
  • ABC8
  • ABCG1
  • ATP-binding cassette sub-family G member 1
  • ATP-binding cassette transporter 8
  • ATP-binding cassette transporter member 1 of subfamily G
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • homolog of Drosophila white
  • MGC34313
  • white protein homolog (ATP-binding cassette transporter 8)
  • White protein homolog
  • WHITE1
  • WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1) is a member of the ABC superfamily of transporters, specifically the ABCG (White) subfamily, that is characterized by their ABC domain organization (1). The ABC domains are around 180 amino acids (aa) in length and contain three conserved domains: Walker A motif (12 aa), Walker B motif (5 aa), and Signature motif (5 aa) (1, 2). Functional ABC transporters are comprised of two ABCs and two transmembrane domains (TMDs), where each TMD contains six TM alpha-helices (1-3). ABCG1 is considered a "half" transporter because it only has one ABC domain and one TMD (1-3). In order to be functional, ABCG1 and other half transporters can form homodimers or heterodimers (1-3). ABCG1 has a theoretical molecular weight of 102 kDa and is highly expressed in cells including macrophages, lymphocytes, and neurons (2). Additionally, ABCG1 is shown to be highly expressed in lung, kidney, brain, and spleen tissue (2). Specifically, ABCG1 is presented on the cell surface and in intracellular compartments of cholesterol-rich macrophages and, consequently, plays a crucial role in cholesterol regulation and homeostasis (3-5). ABCG1 is involved in the export, or efflux, of cholesterol and phospholipids from macrophages to high density lipoproteins (HDL) for eventual excretion via the liver.

A variety of cardiovascular and cardiometabolic diseases are associated with ABCG1 dysfunction (5-7). Macrophages can become cholesterol-containing foam cells that are generated by the uptake of low-density lipoproteins (LDL), cholesterol esterification, and compromised cholesterol efflux machinery in transporters like ABCG1 and ABCA1 (2, 5, 6, 7). Foam cells are associated with the chronic, inflammatory disease atherosclerosis which is characterized by arterial buildup of plaques that can ultimately lead to cardiovascular disease (5, 6, 7). Additionally, ABCG1 has a critical role in cardiometabolic disorders. Studies have found that diabetic mice have decreased ABCG1 expression (8). Furthermore, loss of ABCG1 in mouse pancreatic beta cells ultimately leads to impaired insulin secretion, suggesting that inhibition or modulation of ABCG1 may contribute to development of diabetes and obesity (8). Finally, other related ATP-binding cassette transporter family members, such as ABCA1 and ABCG5/8, have been associated with genetically-inherited syndromes like Tangier disease, characterized by reduced levels of HDL in the blood, and Sitosterolemia, characterized by elevated plant sterol lipid accumulation in blood and tissues (7)..

Alternate names for ABCG1 includes ABC transporter 8 (ABC8), ATP-binding cassette transporter, anti-, sub-family G (WHITE), homolog of Drosophila white, and MGC34313..

References

1. Tarling E. J. (2013). Expanding roles of ABCG1 and sterol transport. Current opinion in lipidology. https://doi.org/10.1097/MOL.0b013e32835da122.

2. Tarr, P. T., Tarling, E. J., Bojanic, D. D., Edwards, P. A., & Baldan, A. (2009). Emerging new paradigms for ABCG transporters. Biochimica et biophysica acta.https://doi.org/10.1016/j.bbalip.2009.01.007.

3. Tarling, E. J., & Edwards, P. A. (2011). ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1113021108.

4. Phillips M. C. (2014). Molecular mechanisms of cellular cholesterol efflux. The Journal of biological chemistry, 289(35), 24020-24029. https://doi.org/10.1074/jbc.R114.583658.

5. Ouimet, M., Barrett, T. J., & Fisher, E. A. (2019). HDL and Reverse Cholesterol Transport. Circulation research. https://doi.org/10.1161/CIRCRESAHA.119.312617.

6. Yu, X. H., Fu, Y. C., Zhang, D. W., Yin, K., & Tang, C. K. (2013). Foam cells in atherosclerosis. Clinica chimica acta; international journal of clinical chemistry. https://doi.org/10.1016/j.cca.2013.06.006

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCG1 Antibody (NB400-132)(145)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chinese Hamster, Hamster, Monkey, Rabbit.

We have publications tested in 7 applications: Flow, ICC/IF, IHC, IHC-P, IP, In vitro, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC
(4)
IHC-P
(1)
IP
(1)
In vitro
(1)
WB
(81)
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Human
(31)
Mouse
(56)
Rat
(6)
Chinese Hamster
(2)
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(1)
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(1)
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All Species
Showing Publications 1 - 10 of 145. Show All 145 Publications.
Publications using NB400-132 Applications Species
Hou X, Zhang C, Wang L, Wang K Natural Piperine Improves Lipid Metabolic Profile of High-Fat Diet-Fed Mice by Upregulating SR-B1 and ABCG8 Transporters Journal of natural products Jan 25 2021 [PMID: 33492139]
Shen C, Jiang J, Huang C, Zhu W. Gypenoside LVI attenuates foam cell formation by promoting cholesterol export and inhibiting inflammation response Oncotarget Oct 5 2018
Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology Dec 14 2020 [PMID: 33381046] (WB, Mouse) WB Mouse
Qian Y, Basu R, Mathes SC et al. Growth Hormone Upregulates Mediators of Melanoma Drug Efflux and Epithelial-to-Mesenchymal Transition In Vitro and In Vivo Cancers Dec 4 2020 [PMID: 33291663] (WB, Mouse, Human) WB Mouse, Human
Zhang X, Rotllan N, CanfrAn-Duque A et al. Targeted suppression of microRNA-33 in lesional macrophages using pH low-insertion peptides (pHLIP) improves atherosclerotic plaque regression Research Square Oct 29 2020 (WB, Mouse) WB Mouse
Po A, Citarella A, Catanzaro G et al. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells Sci Rep Aug 19 2020 [PMID: 32814794] (WB, Human) WB Human
Donadon M, Torzilli G, Cortese N et al. Macrophage morphology correlates with single-cell diversity and prognosis in colorectal liver metastasis J. Exp. Med. Nov 2 2020 [PMID: 32785653] (Flow, Human) Flow Human
Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
Wu A, Grela E, Woojtowicz K, et al. ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells Cell. Mol. Life Sci. May 27 2019 [PMID: 31134303] (WB, Chinese Hamster) WB Chinese Hamster
Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human) WB Human
Show All 145 Publications.

Reviews for ABCG1 Antibody (NB400-132) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB400-132:
Filter by Applications
WB
(2)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Angeliki Chroni
WB Human 10/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
SpeciesHuman
Western Blot ABCG1 NB400-132
Enlarge
5
reviewed by:
Anonymous
WB Mouse 08/26/2013
View

Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsExcellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
Pub Med ID23826352

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG1 Antibody (NB400-132). (Showing 1 - 1 of 1 FAQs).

  1. What secondary antibodies would be recommended for immunofluorescent or Western blot assays when using NB400-132 as the primary antibody?
    • Under the "Support and Research" tab on the NB400-132 product page on the Novus website, there are links to Anti-Rabbit labeled antibodies and isotype controls that may be useful for your experiments.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-132AF350
Alexa Fluor 405 NB400-132AF405
Alexa Fluor 488 NB400-132AF488
Alexa Fluor 532 NB400-132AF532
Alexa Fluor 594 NB400-132AF594
Alexa Fluor 647 NB400-132AF647
Alexa Fluor 700 NB400-132AF700
Alexa Fluor 750 NB400-132AF750
Allophycocyanin/Cy7 NB400-132APCCY7
Biotin NB400-132B
DyLight 350 NB400-132UV
DyLight 405 NB400-132V
DyLight 488 NB400-132G
DyLight 550 NB400-132R
DyLight 594 NB400-132DL594
DyLight 650 NB400-132C
DyLight 680 NB400-132FR
DyLight 755 NB400-132IR
FITC NB400-132F
HRP NB400-132H
Janelia Fluor 549 NB400-132JF549
Janelia Fluor 646 NB400-132JF646
PE/Atto594 NB400-132PEATT594
PE/Cy5.5 NB400-132PECY55
PE/Cy7 NB400-132PECY7

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Blogs on ABCG1.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG1: Easy as 123
ABCG1 (ATP-binding cassette sub-family G member 1) is a transporter protein that is primarily involved in macrophage lipid homeostasis. It is a member of the superfamily of ATP-binding cassette (ABC) transporters and localizes to intracellular compart...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Angeliki Chroni
10/08/2014
Application: WB
Species: Human

Anonymous
08/26/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ABCG1