ABCG1 Antibody - BSA Free


Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last more
Immunohistochemistry-Paraffin: ABCG1 Antibody - BSA Free [NB400-132] - Staining of human lung, alveolar macrophages.
Flow (Intracellular): ABCG1 Antibody - BSA Free [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed more
Biological Strategies: Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Cells were pretreated with 5 uM F12511 or 5 uM MG132 for 30 min and then exposed to 5 or 50 uM 24S-OHC for 6 h. Whole-cell lysates were more
Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.
Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.
Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated macrophages, Lane 3: more
Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected more

Product Details

Reactivity Hu, Mu, Rt, ChHa, Ha, Pm, RbSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IP, WB
BSA Free
1 mg/ml
Validated by:

Biological Strategies


Order Details

ABCG1 Antibody - BSA Free Summary

A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting reported in scientific literature (PMID 28264879)
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23185679)
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation reported in scientific literature (PMID 30235209)
  • In vitro assay
  • In vivo assay reported in scientific literature
  • Western Blot 1:500
Theoretical MW
75.59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
THP-1 TO901317 Treated / Untreated Cell Lysate
Control Peptide
ABCG1 Protein (NB400-132PEP)
Reviewed Applications
Read 2 Reviews rated 5
NB400-132 in the following applications:

Read Publications using
NB400-132 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID: 27230131).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for ABCG1 Antibody - BSA Free

  • ABC transporter 8
  • ABC8
  • ABCG1
  • ATP-binding cassette sub-family G member 1
  • ATP-binding cassette transporter 8
  • ATP-binding cassette transporter member 1 of subfamily G
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • homolog of Drosophila white
  • MGC34313
  • white protein homolog (ATP-binding cassette transporter 8)
  • White protein homolog
  • WHITE1
  • WHT1


ABCG1 (ATP-binding cassette sub-family G member 1) is a member of the ABC superfamily of transporters, specifically the ABCG (White) subfamily, that is characterized by their ABC domain organization (1). The ABC domains are around 180 amino acids (aa) in length and contain three conserved domains: Walker A motif (12 aa), Walker B motif (5 aa), and Signature motif (5 aa) (1, 2). Functional ABC transporters are comprised of two ABCs and two transmembrane domains (TMDs), where each TMD contains six TM alpha-helices (1-3). ABCG1 is considered a "half" transporter because it only has one ABC domain and one TMD (1-3). In order to be functional, ABCG1 and other half transporters can form homodimers or heterodimers (1-3). ABCG1 has a theoretical molecular weight of 102 kDa and is highly expressed in cells including macrophages, lymphocytes, and neurons (2). Additionally, ABCG1 is shown to be highly expressed in lung, kidney, brain, and spleen tissue (2). Specifically, ABCG1 is presented on the cell surface and in intracellular compartments of cholesterol-rich macrophages and, consequently, plays a crucial role in cholesterol regulation and homeostasis (3-5). ABCG1 is involved in the export, or efflux, of cholesterol and phospholipids from macrophages to high density lipoproteins (HDL) for eventual excretion via the liver.

A variety of cardiovascular and cardiometabolic diseases are associated with ABCG1 dysfunction (5-7). Macrophages can become cholesterol-containing foam cells that are generated by the uptake of low-density lipoproteins (LDL), cholesterol esterification, and compromised cholesterol efflux machinery in transporters like ABCG1 and ABCA1 (2, 5, 6, 7). Foam cells are associated with the chronic, inflammatory disease atherosclerosis which is characterized by arterial buildup of plaques that can ultimately lead to cardiovascular disease (5, 6, 7). Additionally, ABCG1 has a critical role in cardiometabolic disorders. Studies have found that diabetic mice have decreased ABCG1 expression (8). Furthermore, loss of ABCG1 in mouse pancreatic beta cells ultimately leads to impaired insulin secretion, suggesting that inhibition or modulation of ABCG1 may contribute to development of diabetes and obesity (8). Finally, other related ATP-binding cassette transporter family members, such as ABCA1 and ABCG5/8, have been associated with genetically-inherited syndromes like Tangier disease, characterized by reduced levels of HDL in the blood, and Sitosterolemia, characterized by elevated plant sterol lipid accumulation in blood and tissues (7). .

Alternate names for ABCG1 includes ABC transporter 8 (ABC8), ATP-binding cassette transporter, anti-, sub-family G (WHITE), homolog of Drosophila white, and MGC34313. .


1. Tarling E. J. (2013). Expanding roles of ABCG1 and sterol transport. Current opinion in lipidology.

2. Tarr, P. T., Tarling, E. J., Bojanic, D. D., Edwards, P. A., & Baldan, A. (2009). Emerging new paradigms for ABCG transporters. Biochimica et biophysica acta.

3. Tarling, E. J., & Edwards, P. A. (2011). ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter. Proceedings of the National Academy of Sciences of the United States of America.

4. Phillips M. C. (2014). Molecular mechanisms of cellular cholesterol efflux. The Journal of biological chemistry, 289(35), 24020-24029.

5. Ouimet, M., Barrett, T. J., & Fisher, E. A. (2019). HDL and Reverse Cholesterol Transport. Circulation research.

6. Yu, X. H., Fu, Y. C., Zhang, D. W., Yin, K., & Tang, C. K. (2013). Foam cells in atherosclerosis. Clinica chimica acta; international journal of clinical chemistry.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
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Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu
Applications: WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Bv, Hu, Rb
Applications: ELISA, ICC/IF, IP, WB
Species: Hu, Mu, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF (-), IHC, IHC-P, WB
Species: Ca, Ch, SyHa, Ha, Hu, Pm, Mu, Rt
Applications: IHC-Fr, KO, Simple Western, WB
Species: Hu
Applications: IHC, IHC-P
Species: Bv, Hu, Po, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA, WB
Species: Bv, Ca, Fe, Hu, Mu, Xp
Applications: ICC/IF, IHC, IHC-Fr, KO, WB

Publications for ABCG1 Antibody (NB400-132)(175)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinese Hamster, Hamster, Monkey, Primate, Rabbit.

We have publications tested in 9 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, In vitro, WB.

Filter By Application
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Showing Publications 1 - 10 of 175. Show All 175 Publications.
Publications using NB400-132 Applications Species
Yalcinkaya M, Fotakis P, Liu W et al. Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1b secretion Cardiovascular research 2022-12-20 [PMID: 36537208] (WB, Mouse) WB Mouse
Bruggmann N Untersuchungen zum ABCA1-/ABCG1-vermittelten Cholesterin-Efflux in humanen Kontroll-und Tangierfibroblasten Thesis 2021-01-01 (WB, Human, Primate) WB Human, Primate
Machado J. T. N-acetylcysteine reduces endoplasmic reticulum stress and affects selectively cholesterol efflux from macrophages mediated by ABCA-1 and ABCG-1 in chronic kidney disease. Thesis. 2014-09-01 (WB, Rat) WB Rat
Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (ICC/IF, IHC-Fr) ICC/IF, IHC-Fr
Gro beta E, Hilger RA, Schümann FL et al. SAM-Competitive EZH2-Inhibitors Induce Platinum Resistance by EZH2-Independent Induction of ABC-Transporters Cancers 2023-06-03 [PMID: 37297005] (WB, Human) WB Human
Wang P, Li M, Gao T et al. Vascular Electrical Stimulation with Wireless, Battery-Free, and Fully Implantable Features Reduces Atherosclerotic Plaque Formation Through Sirt1-Mediated Autophagy Small (Weinheim an der Bergstrasse, Germany) 2023-06-02 [PMID: 37267941] (WB, Mouse) WB Mouse
Cao D, Khan Z, Li X et al. Macrophage angiotensin-converting enzyme (ACE) reduces atherosclerosis by increasing PPAR? and fundamentally changing lipid metabolism Cardiovascular research 2023-05-24 [PMID: 37225143] (WB, Mouse) WB Mouse
Prasad R, Adu-Agyeiwaah Y, Floyd JL et al. Sustained ACE2 Expression by Probiotic Improves Integrity of Intestinal Lymphatics and Retinopathy in Type 1 Diabetic Model Journal of clinical medicine 2023-02-23 [PMID: 36902558] (IHC-P, Mouse) IHC-P Mouse
Cassim Bawa FN, Gopoju R, Xu Y et al. Retinoic Acid Receptor Alpha (RARa) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice Cells 2022-10-11 [PMID: 36291054] (WB, Mouse) WB Mouse
Xu H, Zheng LX, Chen XS et al. Brain-specific loss of Abcg1 disturbs cholesterol metabolism and aggravates pyroptosis and neurological deficits after traumatic brain injury Brain Pathology 2023-05-01 [PMID: 36271611] (ICC/IF, IHC) ICC/IF, IHC
Show All 175 Publications.

Reviews for ABCG1 Antibody (NB400-132) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB400-132:
Filter by Applications
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Filter by Species
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Images Ratings Applications Species Date Details
reviewed by:
Angeliki Chroni
WB Human 10/08/2014


ApplicationWestern Blot
Sample TestedABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
reviewed by:
Verified Customer
WB Mouse 08/26/2013


ApplicationWestern Blot

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG1 Antibody (NB400-132). (Showing 1 - 1 of 1 FAQs).

  1. What secondary antibodies would be recommended for immunofluorescent or Western blot assays when using NB400-132 as the primary antibody?
    • Under the "Support and Research" tab on the NB400-132 product page on the Novus website, there are links to Anti-Rabbit labeled antibodies and isotype controls that may be useful for your experiments.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG1: Easy as 123
ABCG1 (ATP-binding cassette sub-family G member 1) is a transporter protein that is primarily involved in macrophage lipid homeostasis. It is a member of the superfamily of ATP-binding cassette (ABC) transporters and localizes to intracellular compart...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

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Recent Reviews


Angeliki Chroni
Application: WB
Species: Human

Verified Customer
Application: WB
Species: Mouse


Gene Symbol ABCG1