ABCG1 Antibody

Images

 
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Ln1: positive ABCG1 control, 22-(R)-hydrocholesterol treated macrophages (2), T0901713 treated macrophages (3), and T091713 ...read more
Immunohistochemistry: ABCG1 Antibody [NB400-132] - Analysis using the Biotin conjugate of NB400-132. Staining of lung, alveolar macrophages.
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.
Western Blot: ABCG1 Antibody [NB400-132] - Western blot analysis of Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at a concentration of 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Ha, Mk, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml

ABCG1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • In vitro assay
Application Notes
This ABCG1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) were tested, where a band was observed at ~90 kDa. Preliminary results suggest that this antibody does not appear to work in Immunoprecipitation.In vivo assay was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23185679)
Control Peptide
ABCG1 Peptide (NB400-132PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB400-132 in the following applications:

Publications
Read Publications using
NB400-132 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID; 23185679). Chinese Hamster, and Monkey reactivity reported in scientific literature (PMID: 27230131)

Alternate Names for ABCG1 Antibody

  • ABC transporter 8
  • ABC8ATP-binding cassette sub-family G member 1
  • ATP-binding cassette transporter 8
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • homolog of Drosophila white
  • MGC34313
  • white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G
  • White protein homolog
  • WHITE1
  • WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 involves in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-132
Species: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
Applications: WB, ICC/IF, IHC, IHC-P, In vitro

Publications for ABCG1 Antibody (NB400-132)(102)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chinese Hamster, Hamster, Monkey, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, In vitro, WB.


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ICC/IF
(2)
IHC
(3)
IHC-P
(1)
In vitro
(1)
WB
(52)
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Human
(19)
Mouse
(35)
Rat
(3)
Chinese Hamster
(1)
Hamster
(1)
Monkey
(1)
Rabbit
(1)
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Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using NB400-132 Applications Species
Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 12:00AM [PMID:27890613] (Mouse) Mouse
Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 12:00AM [PMID:27862930] (WB, Human) WB Human
Cheng HY, Gaddis DE, Wu R et al. Loss of ABCG1 influences regulatory T cell differentiation and atherosclerosis. J. Clin. Invest. Sep 1 2016 12:00AM [PMID:27482882] (WB, Mouse) WB Mouse
Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID:27471270] (WB, Mouse) WB Mouse
Aryal B, Rotllan N, Araldi E et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. Jul 27 2016 12:00AM [PMID:27460411] (WB, Mouse) WB Mouse
Mostafa A, Hamdy N, Abdel-Rahman S, El-Mesallamy H. Effect of vildagliptin and pravastatin combination on cholesterol efflux in adipocytes. IUBMB Life. [PMID:27251372] (WB, Mouse) WB Mouse
Tarling EJ, Edwards PA. Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both ABCG1-L550 and ABCG1-P550. Arterioscler. Thromb. Vasc. Biol. May 26 2016 12:00AM [PMID:27230131] (WB, ICC/IF, Mouse, Chinese Hamster, Monkey) WB, ICC/IF Mouse, Chinese Hamster, Monkey
Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID:27114256] (WB, Mouse) WB Mouse
Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID:27063951] (WB, Mouse) WB Mouse
Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 12:00AM [PMID:26821916] (WB, Mouse) WB Mouse
Show All 102 Publications.

Reviews for ABCG1 Antibody (NB400-132) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB400-132:
Filter by Applications
WB
(2)
All Applications
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Angeliki Chroni
WB Human 10/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
SpeciesHuman
Western Blot ABCG1 NB400-132
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reviewed by:
Anonymous
WB Mouse 08/26/2013
View

Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsExcellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
Pub Med ID23826352

Product General Protocols

View specific protocols for ABCG1 Antibody (NB400-132): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG1 Antibody (NB400-132) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-132AF405
Alexa Fluor 488 Labeled NB400-132AF488
Alexa Fluor 647 Labeled NB400-132AF647
Alexa Fluor 700 Labeled NB400-132AF700
Biotin Labeled NB400-132B
DyLight 405 Labeled NB400-132V
DyLight 488 Labeled NB400-132G
DyLight 550 Labeled NB400-132R
DyLight 650 Labeled NB400-132C
FITC Labeled NB400-132F
HRP Labeled NB400-132H

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Blogs on ABCG1.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG1: Easy as 123
ABCG1 (ATP-binding cassette sub-family G member 1) is a transporter protein that is primarily involved in macrophage lipid homeostasis. It is a member of the superfamily of ATP-binding cassette (ABC) transporters and localizes to intracellular compart...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Angeliki Chroni
10/08/2014
Application: WB
Species: Human

Anonymous
08/26/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ABCG1

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