ABCG1 Antibody

Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated macrophages, Lane 3: T0901713 treated macrophages, Lane 4: T091713 treated macrophages.

Flow (Intracellular): ABCG1 Antibody [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody.

Western Blot: ABCG1 Antibody [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: ABCG1 Antibody [NB400-132] - Staining of human lung, alveolar macrophages.

Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.

Western Blot: ABCG1 Antibody [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.

Western Blot: ABCG1 Antibody [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected subcutaneously with PBS or 0.8 ug/g LPS as indicated, followed 4 hr later by intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages. ABCG1 in liver lysates (40 ug protein) was identified by immunoblotting and quantified by densitometry. Results from each of the 3 experiments (n = 4-5 per group per experiment) were expressed relative to PBS-injected WT mice after normalization to beta-actin. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/283486/), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, ChHa, Ha, Pm, Rb
• Applications: WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, In vitro, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ABCG1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation
  • In vitro assay
  • Flow (Intracellular)
• Application Notes: Preliminary results suggest that this antibody does not work in IP. In vivo assay was reported in scientific literature. Use in ICC/IF reported in scientific literature (PMID 23185679). Use in immunoblotting reported in scientific literature (PMID: 28264879). Use in immunoprecipitation reported in scientific literature (PMID:30235209).
• Theoretical MW: 75.59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ABCG1 Protein (NB400-132PEP)

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID 23185679). Chinese Hamster and Monkey reactivity reported in scientific literature (PMID 27230131).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCG1 Antibody

• ABC transporter 8
• ABC8
• ABCG1
• ATP-binding cassette sub-family G member 1
• ATP-binding cassette transporter 8
• ATP-binding cassette transporter member 1 of subfamily G
• ATP-binding cassette, sub-family G (WHITE), member 1
• homolog of Drosophila white
• MGC34313
• white protein homolog (ATP-binding cassette transporter 8)
• White protein homolog
• WHITE1
• WHT1

Background

ABCG1 (ATP-binding cassette sub-family G member 1) is a member of the ABC superfamily of transporters, specifically the ABCG (White) subfamily, that is characterized by their ABC domain organization (1). The ABC domains are around 180 amino acids (aa) in length and contain three conserved domains: Walker A motif (12 aa), Walker B motif (5 aa), and Signature motif (5 aa) (1, 2). Functional ABC transporters are comprised of two ABCs and two transmembrane domains (TMDs), where each TMD contains six TM alpha-helices (1-3). ABCG1 is considered a "half" transporter because it only has one ABC domain and one TMD (1-3). In order to be functional, ABCG1 and other half transporters can form homodimers or heterodimers (1-3). ABCG1 has a theoretical molecular weight of 102 kDa and is highly expressed in cells including macrophages, lymphocytes, and neurons (2). Additionally, ABCG1 is shown to be highly expressed in lung, kidney, brain, and spleen tissue (2). Specifically, ABCG1 is presented on the cell surface and in intracellular compartments of cholesterol-rich macrophages and, consequently, plays a crucial role in cholesterol regulation and homeostasis (3-5). ABCG1 is involved in the export, or efflux, of cholesterol and phospholipids from macrophages to high density lipoproteins (HDL) for eventual excretion via the liver.

A variety of cardiovascular and cardiometabolic diseases are associated with ABCG1 dysfunction (5-7). Macrophages can become cholesterol-containing foam cells that are generated by the uptake of low-density lipoproteins (LDL), cholesterol esterification, and compromised cholesterol efflux machinery in transporters like ABCG1 and ABCA1 (2, 5, 6, 7). Foam cells are associated with the chronic, inflammatory disease atherosclerosis which is characterized by arterial buildup of plaques that can ultimately lead to cardiovascular disease (5, 6, 7). Additionally, ABCG1 has a critical role in cardiometabolic disorders. Studies have found that diabetic mice have decreased ABCG1 expression (8). Furthermore, loss of ABCG1 in mouse pancreatic beta cells ultimately leads to impaired insulin secretion, suggesting that inhibition or modulation of ABCG1 may contribute to development of diabetes and obesity (8). Finally, other related ATP-binding cassette transporter family members, such as ABCA1 and ABCG5/8, have been associated with genetically-inherited syndromes like Tangier disease, characterized by reduced levels of HDL in the blood, and Sitosterolemia, characterized by elevated plant sterol lipid accumulation in blood and tissues (7)..

Alternate names for ABCG1 includes ABC transporter 8 (ABC8), ATP-binding cassette transporter, anti-, sub-family G (WHITE), homolog of Drosophila white, and MGC34313..

References

1. Tarling E. J. (2013). Expanding roles of ABCG1 and sterol transport. Current opinion in lipidology. https://doi.org/10.1097/MOL.0b013e32835da122.

2. Tarr, P. T., Tarling, E. J., Bojanic, D. D., Edwards, P. A., & Baldan, A. (2009). Emerging new paradigms for ABCG transporters. Biochimica et biophysica acta.https://doi.org/10.1016/j.bbalip.2009.01.007.

3. Tarling, E. J., & Edwards, P. A. (2011). ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1113021108.

4. Phillips M. C. (2014). Molecular mechanisms of cellular cholesterol efflux. The Journal of biological chemistry, 289(35), 24020-24029. https://doi.org/10.1074/jbc.R114.583658.

5. Ouimet, M., Barrett, T. J., & Fisher, E. A. (2019). HDL and Reverse Cholesterol Transport. Circulation research. https://doi.org/10.1161/CIRCRESAHA.119.312617.

6. Yu, X. H., Fu, Y. C., Zhang, D. W., Yin, K., & Tang, C. K. (2013). Foam cells in atherosclerosis. Clinica chimica acta; international journal of clinical chemistry. https://doi.org/10.1016/j.cca.2013.06.006

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG1 Antibody (NB400-132)(145)

Publications using NB400-132

• Hou X, Zhang C, Wang L, Wang K Natural Piperine Improves Lipid Metabolic Profile of High-Fat Diet-Fed Mice by Upregulating SR-B1 and ABCG8 Transporters Journal of natural products Jan 25 2021 [PMID: 33492139]
• Shen C, Jiang J, Huang C, Zhu W. Gypenoside LVI attenuates foam cell formation by promoting cholesterol export and inhibiting inflammation response Oncotarget Oct 5 2018
• Ma C, Zhang J, Yang S et al. Astragalus Flavone Ameliorates Atherosclerosis and Hepatic Steatosis Via Inhibiting Lipid-Disorder and Inflammation in apoE-/- Mice Frontiers in Pharmacology Dec 14 2020 [PMID: 33381046] (WB, Mouse)
• Qian Y, Basu R, Mathes SC et al. Growth Hormone Upregulates Mediators of Melanoma Drug Efflux and Epithelial-to-Mesenchymal Transition In Vitro and In Vivo Cancers Dec 4 2020 [PMID: 33291663] (WB, Mouse, Human)
• Zhang X, Rotllan N, CanfrAn-Duque A et al. Targeted suppression of microRNA-33 in lesional macrophages using pH low-insertion peptides (pHLIP) improves atherosclerotic plaque regression Research Square Oct 29 2020 (WB, Mouse)
• Po A, Citarella A, Catanzaro G et al. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells Sci Rep Aug 19 2020 [PMID: 32814794] (WB, Human)
• Donadon M, Torzilli G, Cortese N et al. Macrophage morphology correlates with single-cell diversity and prognosis in colorectal liver metastasis J. Exp. Med. Nov 2 2020 [PMID: 32785653] (Flow, Human)
• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
• Wu A, Grela E, Woojtowicz K, et al. ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells Cell. Mol. Life Sci. May 27 2019 [PMID: 31134303] (WB, Chinese Hamster)
• Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human)
• Shen C, Jiang J, Huang C, Zhu W. Gypenoside LVI attenuates foam cell formation by promoting cholesterol export and inhibiting inflammation response Journal of Functional Foods Nov 1 2018 (WB, Mouse)
• Barilla S, Liang N, Mileti E et al. Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling through upregulation of ATP binding cassette subfamily G member 1 Mol Metab Aug 13 2020 [PMID: 32798719] (WB, Human)
• Koga M, Kanaoka Y, Okamoto M et al. Varenicline aggravates atherosclerotic plaque formation in nicotine-pretreated ApoE knockout mice due to enhanced oxLDL uptake by macrophages through downregulation of ABCA1 and ABCG1 expression J. Pharmacol. Sci. Nov 14 2019 [PMID: 31771811] (WB, Mouse)
• Urano Y, Ho Vo DK, Hirofumi A, Noguchi N 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death Cell Death Discov Jul 5 2019 [PMID: 31285856] (WB, Human)
• Pasca AM, Park JY, Shin HW et al. Cyclodextrin Ameliorates the Progression of Atherosclerosis via Increasing High-Density Lipoprotein Cholesterol Plasma Levels and Anti-inflammatory Effects in Rabbits J. Cardiovasc. Pharmacol. May 1 2019 [PMID: 30855405]
• Sedgeman LR, Beysen C, Ramirez Solano MA et al. Beta cell secretion of miR-375 to HDL is inversely associated with insulin secretion Sci Rep Mar 7 2019 [PMID: 30846744] (WB, Mouse)
• Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019 Jan [PMID: 30389579] (Mouse)
• Nishino T, Horie T, Baba O et al. SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo Arteriosclerosis, Thrombosis, and Vascular Biology [PMID: 30354203] (WB, Mouse)
• Harris MT, Hussain SS, Inouye CM et al. Reinterpretation of the localization of the ATP binding cassette transporter ABCG1 in insulin-secreting cells and insights regarding its trafficking and function PLoS One. 2018 Sep 19 [PMID: 30235209] (IP, Rat)
• Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International Jul 12 2018 [PMID: 30105261] (WB, Rabbit)
• Wang L, Eftekhari P, Schachner D et al. Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux. Sci Rep. Jul 23 2018 [PMID: 30038271] (WB, Human)
• Sonett J, Goldklang M, Sklepkiewicz P et al. A critical role for ABC transporters in persistent lung inflammation in the development of emphysema after smoke exposure. FASEB J 2018 Jun 15 [PMID: 29906247] (Mouse)
• Ying F, Cai Y, Wong HK et al. EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia Biochim Biophys Acta Mol Cell Biol Lipids. [PMID: 29890224] (WB, Mouse)
• Wang D, Hiebl V, Ladurner A et al. 6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-Derived Macrophages. Mol Nutr Food Res 2018 May 26 [PMID: 29802792] (Human)
• Li Y, Shen S, Ding S, Wang L. Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor-kappa B pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis Experimental and Therapeutic Medicine 2017 Oct 31 [PMID: 29375683] (WB, Human)
• Westerterp M, Tsuchiya K et al. Deficiency of ATP-Binding Cassette Transporters A1 and G1 in Endothelial Cells Accelerates Atherosclerosis in Mice. Arterioscler Thromb Vasc Biol Jan 7 2016 [PMID: 27199450] (WB, Mouse)
• Ren Q, Xie X, Tang Y et al. Methyl tertiary-butyl ether inhibits THP-1 macrophage cholesterol efflux in vitro and accelerates atherosclerosis in ApoE-deficient mice in vivo Journal of Environmental Sciences Mar 1 2020 (WB, Mouse)
• L Marko, G Paragh, P Ugocsai et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light Biology 2012 (WB, IHC, Human)
• Price NL, Zhang X, Fernández-Tussy P, et al. Loss of hepatic miR-33 improves metabolic homeostasis and liver function without altering body weight or atherosclerosis Proceedings of the National Academy of Sciences of the United States of America Feb 2 2021 [PMID: 33495342] (WB, Mouse)
• Shang Y, Ma C, Zhang J et al. Bifunctional supramolecular nanofiber inhibits atherosclerosis by enhancing plaque stability and anti-inflammation in apoE-/- mice Theranostics Aug 13 2020 [PMID: 32929345] (WB, Mouse)
• Gao Q, Wei A, Chen F et al. Enhancing PPAR gamma by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice Pharmacological Research Jul 1 2020 [PMID: 32621955] (WB, Mouse)
• Werkman IL, KOvilein J, de Jonge JC, Baron W Impairing committed cholesterol biosynthesis in white matter astrocytes, but not grey matter astrocytes, enhances in vitro myelination J. Neurochem. Jun 30 2020 [PMID: 32602556] (WB, Rat)
• Chen LW, Tsai MC, Chern CY et al. A chalcone derivative, 1m-6, exhibits atheroprotective effects by increasing cholesterol efflux and reducing inflammation-induced endothelial dysfunction Br. J. Pharmacol. Jun 24 2020 [PMID: 32579243]
• Wang D, Hiebl V, Schachner D et al. Soraphen A enhances macrophage cholesterol efflux via indirect LXR activation and ABCA1 upregulation Biochem. Pharmacol. May 8 2020 [PMID: 32437644] (WB, Human)
• Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020 May 21 [PMID: 32437392] (WB, Human)
• Shen CY, Wang TX, Jiang JG et al. Bergaptol from blossoms of Citrus aurantium L. var. amara Engl inhibits LPS-induced inflammatory responses and ox-LDL-induced lipid deposition Food Funct May 20 2020 [PMID: 32432251]
• Salonurmi T, Nabil H, Ronkainen J et Al. 5 beta-Hydroxycholesterol Signals From the Liver to Regulate Peripheral Cholesterol Transporters Front. Pharmacol. Mar 27 2020 [PMID: 32292343] (WB, Rat)
• Koga M, Kanaoka Y, Inada K et al. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci. Feb 28 2020 [PMID: 32173266] (WB, Mouse)
• Guo Y MicroRNA-205-5p Promotes Unstable Atherosclerotic Plaque Formation In Vivo Cardiovasc Drugs Ther Feb 8 2020 [PMID: 32034643] (Mouse)
• Cuffe H, Liu M, Key CC et al. Targeted Deletion of Adipocyte Abca1 (ATP-Binding Cassette Transporter A1) Impairs Diet-Induced Obesity Arterioscler. Thromb. Vasc. Biol. 2018 Jan 18 [PMID: 29348118] (Mouse)
• Canfran-Duque A, Rotllan N, Zhang X et al. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis EMBO Mol Med 2017 Jul 03 [PMID: 28674080] (WB, Mouse)
• de Aguiar Vallim TQ, Lee E, Merriott DJ et al. ABCG1 regulates pulmonary surfactant metabolism in mice and men. J. Lipid Res. Mar 6 2017 [PMID: 28264879]
• Mariani MM, Malm T, Lamb R et al. Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease. Sci Rep. 2017 Feb 16 [PMID: 28205585] (WB, Mouse)
• Jiang M, Li X. Activation of PPARgamma does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI. Biochem. Biophys. Res. Commun. Nov 24 2016 [PMID: 27890613] (Mouse)
• Wang L, Palme V, Rotter S et al. Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Mol Nutr Food Res. Nov 15 2016 [PMID: 27862930] (WB, Human)
• Alsina-Sachis E, Muelfarth R, Moll I et al. Intraperitoneal oil application causes local inflammation with depletion of resident peritoneal macrophages bioRxiv Jul 16 2020 (WB, Mouse)
• Cheng HY, Gaddis DE, Wu R et al. Loss of ABCG1 influences regulatory T cell differentiation and atherosclerosis. J. Clin. Invest. Sep 1 2016 [PMID: 27482882] (WB, Mouse)
• Lai L, Azzam KM, Lin W et al. MicroRNA-33 regulates the innate immune response via ATP Binding Cassette transporter-mediated remodeling of membrane microdomains. J. Biol. Chem. [PMID: 27471270] (WB, Mouse)
• Aryal B, Rotllan N, Araldi E et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. Jul 27 2016 [PMID: 27460411] (WB, Mouse)
• Mostafa A, Hamdy N, Abdel-Rahman S, El-Mesallamy H. Effect of vildagliptin and pravastatin combination on cholesterol efflux in adipocytes. IUBMB Life. [PMID: 27251372] (WB, Mouse)
• Tarling EJ, Edwards PA. Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both ABCG1-L550 and ABCG1-P550. Arterioscler. Thromb. Vasc. Biol. May 26 2016 [PMID: 27230131] (WB, ICC/IF, Mouse, Chinese Hamster, Monkey)
• Zhang X, Hu J, Zhong L et al. Quercetin stabilizes apolipoprotein E and reduces brain Abeta levels in amyloid model mice. Neuropharmacology. 2016 Apr 22 [PMID: 27114256] (WB, Mouse)
• Leng S, Iwanowycz S, Saaoud F et al. Ursolic acid enhances macrophage autophagy and attenuates atherogenesis. J. Lipid Res. 2016 Apr 10 [PMID: 27063951] (WB, Mouse)
• Shi H, Mao X, Zhong Y et al. Lanatoside C Promotes Foam Cell Formation and Atherosclerosis Sci Rep Jan 29 2016 [PMID: 26821916] (WB, Mouse)
• Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ. Glucagon-like peptide 1 (GLP-1)-based therapy upregulate LXR-ABCA1/ABCG1 cascade in adipocytes. Biochem. Biophys. Res. Commun. 2015 Nov 18 [PMID: 26603933]
• Matsuo K, Yamamoto S, Wakamatsu T et al. Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate. Toxins (Basel). 2015 Aug 20 [PMID: 26287243] (WB, Human)
  
  Details:
  ABCG1 antibody was used at 1:1000 dilution for WB analysis of lysates from THP-1 cells that were exposed to 1 mM indoxyl sulfate for differentiation for 24 hours (Figure 2).
• Daffu G, Shen X, Senatus L et al. RAGE Suppresses ABCG1-Mediated Macrophage Cholesterol Efflux in Diabetes. Diabetes. 2015 Aug 07 [PMID: 26253613]
• Lee Tzong-Shyuan, Lu Kuo-Yun, Yu Yuan-Bin et al. B Common Receptor Mediates Erythropoietin-Conferred Protection on OxLDL-Induced Lipid Accumulation and Inflammation in Macrophages. Mediators of Inflammation 2015 [PMID: 26101463] (IHC, Mouse)
• De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015 Feb 21 [PMID: 25708389] (WB, Mouse)
• Kuo Cheng-Hsiang, Leon Lorraine, Chung Eun Ji et al. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B Mater Biol Med. 2014 Dec 14 [PMID: 25685357]
• Park SH, Paek JH, Shin D et al. Purple perilla extracts with alpha-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages Int. J. Mol. Med. 2015 Feb 12 [PMID: 25673178] (WB, Mouse)
• Du X M, Kim M J et al. HDL particle size is a critical determinant of ABCA1-mediated macrophage cellular cholesterol export. Circ Res Mar 27 2015 [PMID: 25589556] (WB, Human)
• The expression of ABCG1 transporter gene in peripheral blood mononuclear cells of patients with atherosclerosis. Miroshnikova VV, Demina EP, Mayorov NV Tsitologiia Jul 6 2005 [PMID: 25509420]
• Zheng W, Mast N, Saadane A, Pikuleva Ia. Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments. J. Lipid Res. 2014 Oct 07 [PMID: 25293590] (IHC, Mouse)
• Frisdal E, Lay SL, Hooton H et al. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity. Diabetes. 2014 Sep 23 [PMID: 25249572]
• Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014 Sep 12 [PMID: 25217640]
• Horie Takahiro, Nishino Tomohiro, Baba Osamu et al. MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. Sci Rep. 2014 Jun 16 [PMID: 24931346] (Mouse)
• Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 3/5/2014 [PMID: 24637412] (WB, Mouse)
• Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013 Dec 4 [PMID: 24371802] (WB, Human)
• Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013 Nov 6 [PMID: 24223214] (WB, Mouse)
• Nicod N, Parker RS. Vitamin E Secretion by Caco-2 Monolayers to APOA1, but Not to HDL, Is Vitamer Selective. J Nutr. 2013 Oct [PMID: 23946344] (WB, Human)
• Daniil G, Zannis VI, Chroni A. Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux. PLoS One 2013 Jun 27 [PMID: 23826352] (WB, Mouse, Human)
• Zhou MS, Chadipiralla K, Mendez AJ et al. Nicotine potentiates proatherogenic effects of oxLDL by stimulating and upregulating macrophage CD36 signaling. Am J Physiol Heart Circ Physiol. 2013 Aug [PMID: 23748423] (WB, Human)
• Nakaya K, Ayaori M, Uto-Kondo H et al. Overexpression of stearoyl-coenzyme A desaturase 1 in macrophages promotes reverse cholesterol transport. Biochim Biophys Acta 2013 Aug [PMID: 23747827] (WB, Mouse)
• Fang L, Choi SH, Baek JS et al. Control of angiogenesis by AIBP-mediated cholesterol efflux. Nature 2013 Jun 6 [PMID: 23719382] (WB, Human)
• Doddapattar P, Radovic B, Patankar JV et al. Xanthohumol ameliorates atherosclerotic plaque formation, hypercholesterolemia, and hepatic steatosis in ApoE-deficient mice. Mol Nutr Food Res 2013 May 3 [PMID: 23650230] (WB, Mouse)
• Mulay V, Wood P, Manetsch M et al. Inhibition of Mitogen-Activated Protein Kinase Erk1/2 Promotes Protein Degradation of ATP Binding Cassette Transporters A1 and G1 in CHO and HuH7 Cells. PLoS One 2013 Apr 25 [PMID: 23634230] (WB, Hamster, Human)
• de Beer MC, Wroblewski JM, Noffsinger VP et al. The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A. J Lipids 2013 [PMID: 23431457] (WB, Mouse)
• Huang CX, Zhang YL, Wang Jf et al. MCP-1 impacts RCT by repressing ABCA1, ABCG1, and SR-BI through PI3K/Akt posttranslational regulation in HepG2 cells. J Lipid Res 2013 May [PMID: 23402987]
• Gu HM, Li G, Gao X, Berthiaume LG, Zhang DW. Characterization of palmitoylation of ATP binding cassette transporter G1: effect on protein trafficking and function. Biochim Biophys Acta 2013 Feb 3 [PMID: 23388354]
• El Roz A, Bard JM, Huvelin JM, Nazih H. The anti-proliferative and pro-apoptotic effects of the trans9,trans11 conjugated linoleic acid isomer on MCF-7 breast cancer cells are associated with LXR activation. Prostaglandins Leukot Essent Fatty Acids 2013 Jan 31 [PMID: 23375583] (WB, Human)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013 Feb 19 [PMID: 23334894] (WB, Mouse)
• Horie T, Baba O, Kuwabara Y et al. MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice. J Am Heart Assoc 2012 [PMID: 23316322] (WB, Mouse)
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation Biochem Pharmacol 2013 Jan 9 [PMID: 23313547] (WB, Human)
• Machado J. T. N-acetylcysteine reduces endoplasmic reticulum stress and affects selectively cholesterol efflux from macrophages mediated by ABCA-1 and ABCG-1 in chronic kidney disease. Thesis. 2014 Sep 01 (WB, Rat)
• Munch G, Bultmann A, Li Z et al. Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits. Heart Int 2012 Jun 5 [PMID: 23185679] (ICC/IF, Rabbit)
• Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013 Jan [PMID: 23117655] (WB, Mouse)
• Chen J, Zhang X, Kusumo H et al. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis Biochim Biophys Acta 2012 Sep 23 [PMID: 23010475] (WB, Rat)
• Marko L, Paragh G, Ugocsai P et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light J Photochem Photobiol B 2012 Nov 5 [PMID: 22982209] (WB, IHC, Human)
• Gelissen IC, Sharpe LJ, Sandoval C et al. Protein kinase A modulates the activity of a major human isoform of ABCG1 J Lipid Res 2012 Oct [PMID: 22872754]
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Reviews for ABCG1 Antibody (NB400-132) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Angeliki Chroni WB Human 10/08/2014

Summary

• Application: Western Blot
• Sample Tested: ABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
• Species: Human

reviewed by: Anonymous WB Mouse 08/26/2013

Summary

• Application: Western Blot
• Species: Mouse
• Special Applications: Excellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
• Pub Med ID: 23826352

FAQs for ABCG1 Antibody (NB400-132). (Showing 1 - 1 of 1 FAQs).

1. What secondary antibodies would be recommended for immunofluorescent or Western blot assays when using NB400-132 as the primary antibody?
   • Under the "Support and Research" tab on the NB400-132 product page on the Novus website, there are links to Anti-Rabbit labeled antibodies and isotype controls that may be useful for your experiments.

Bioinformatics

• Gene Symbol: ABCG1