SREBP2: From Cholesterol Homeostasis to Cancer Invasion

Wed, 06/06/2012 - 09:31

Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP2 modulates expression of the transmembrane protein ABCA1, which is responsible for managing cellular cholesterol efflux. ABCA1 mediates transport of lipids between the Golgi body and plasma membrane, as well as cholesterol and phospholipid efflux to the ApoA1 and ApoE apolipoproteins in the formation of HDL. Studies with SREBP antibodies have shown that other SREBP family isoforms have different roles in lipid synthesis – SREBP2 however, is relatively specific to cholesterol synthesis1.

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody

Recent work with SREBP antibodies in smooth muscle cells studied the effects of adhesion on HMG-CoA reductase as the rate-limiting step within the cholesterol biosynthesis pathway2. Exciting and new chromatin immunuprecipitation studies with SREBP2 antibody found that cholesterol synthesis signal transduction pathways (like that of SREBP) may have a strong role in cancer invasion3. Specifically, HDAC5 was found to be a downstream SREBP2 target that not only upregulates oxysterol binding proteins but is associated with tumor invasion and poor prognosis in pancreatic cancer patients. miRNAs have been shown to play an important role in cholesterol homeostasis by post-transcriptional regulation of a number of lipid metabolism genes, with SREBP antibody studies showing ABCA1 inhibition through a powerful ntronic miRNA named miR-33 that affects several other lipids 4.

  1. PMID: 15589694
  2. PMID: 19608976
  3. PMID: 20128820
  4. PMID: 20732877

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For cancer invasion this blog gave an idea about how to calculate from cholestrol.Thanks for giving this information.

Thanks for sharing your thoughts on abca1. Regards

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