CD36 Antibody - BSA Free

Immunohistochemistry: CD36 Antibody [NB400-144] - Timp4-deficiency results in defective lipid digestion and absorption. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed and HFD-fed WT and Timp4-/- mice. Western blot (C) and mRNA (D) for CD36 in enterocyte fraction of chow-fed and HFD-fed WT and Timp4-/- mice (collected from the proximal small intestine). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY licence.

Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of CD36 in human adipocytes.

Western Blot: CD36 Antibody [NB400-144] - Mouse primary muscle cell lysate. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD36 Antibody [NB400-144] - Bone marrrow derived mouse macrophages stained with CD36 antibody. ICC/IF image submitted by a verified customer review.

Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Rb
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD36 Antibody - BSA Free Summary

• Immunogen: This CD36 Antibody was developed against a synthetic peptide mapping to a region of human CD36 between residues 100-200 [Uniprot# P16671]
• Localization: Integral membrane
• Marker: Endothelial Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD36
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24531551). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CD36 Overexpression Lysate
  Human Adipose Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  CD36 Protein (NB400-144PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34974159) Porcine reactivity reported in scientific literature (PMID: 23727393). Rat reactivity reported in scientific literature (PMID: 25635851). Rabbit reactivity reported in scientific literature (PMID: 30105261)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD36 Antibody - BSA Free

• BDPLT10
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 molecule (thrombospondin receptor)
• CHDS7
• cluster determinant 36
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIB
• GPIV
• leukocyte differentiation antigen CD36
• PAS IV
• PAS-4 protein
• PASIV
• platelet glycoprotein 4
• platelet glycoprotein IV
• SCARB3
• scavenger receptor class B, member 3

Background

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD36 Antibody (NB400-144)(100)

Publications using NB400-144

• Kim D, Jang G, Hwang J et al. Combined Therapy of Low-Dose Angiotensin Receptor-Neprilysin Inhibitor and Sodium-Glucose Cotransporter-2 Inhibitor Prevents Doxorubicin-Induced Cardiac Dysfunction in Rodent Model with Minimal Adverse Effects Pharmaceutics 2022-11-28 [PMID: 36559124] (WB, Mouse)
• Jabbari K, Cheng Q, Winkelmaier G Et al. CD36(+) Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast Int J Mol Sci 2022-11-11 [PMID: 36361532] (ICC/IF, Human)
  
  Details:
  Citation using the FITC version of this antibody.
• Liao M, Li Y, Xiao A et al. HIF-2a-induced upregulation of CD36 promotes the development of ccRCC Experimental cell research 2022-10-15 [PMID: 36252650] (WB, Human)
• Lin YC, Lin HF, Wu CC et al. Pathogenic effects of Desulfovibrio in the gut on fatty liver in diet-induced obese mice and children with obesity Journal of gastroenterology 2022-08-17 [PMID: 35976494]
• Nakamura NK, Tokunaga DS, Ha HY, Polgar N The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells Cells 2022-08-06 [PMID: 35954283] (ICC/IF, PLA, Rat)
  
  Details:
  L6-GLUT4myc cells
• Daquinag Ac, Gao Z, Fussell C Et Al. Fatty acid mobilization from adipose tissue is mediated by CD36 post-translational modifications and intracellular trafficking JCI insight 2021-07-27 [PMID: 34314388] (WB)
• Trivedi P ROLE OF TRANSCRIPTION FACTOR EB (TFEB) IN CARDIAC HEALTH AND DISEASE Thesis 2020-01-01 (WB, Rat)
• Jin Y, Liu Y, Xu L et al. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis Cell death & disease 2022-05-31 [PMID: 35641492] (WB, Mouse)
• Blachnio-Zabielska AU, Roszczyc-Owsiejczuk K, Imierska M et al. CerS1 but Not CerS5 Gene Silencing, Improves Insulin Sensitivity and Glucose Uptake in Skeletal Muscle Cells 2022-01-08 [PMID: 35053322] (WB, Mouse)
• Zeng H, Qin H, Liao M et al. CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing Molecular metabolism 2021-12-30 [PMID: 34974159] (WB, Mouse)
• Bhat N, Narayanan A, Fathzadeh M et al. Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice The Journal of clinical investigation 2021-12-02 [PMID: 34855620] (WB)
• Wang J, Qin X, Sun H Et al. Nogo receptor impairs the clearance of fibril amyloid-beta by microglia and accelerates Alzheimer's-like disease progression Aging cell 2021-11-24 [PMID: 34821024] (WB, Mouse)
• Jabbari K, Winkelmaier G, Andersen C et al. Protein Ligands in the Secretome of CD36+ Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines Cancers 2021-09-08 [PMID: 34572749] (ICC/IF, Human)
• Fujimoto BA, Young M, Nakamura N et al. Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model The Journal of biological chemistry 2021-02-26 [PMID: 33647317] (WB, Mouse)
• Jayakumari NR, Rajendran RS, Sivasailam A, et al. Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart European journal of pharmacology 2021-01-30 [PMID: 33529726] (WB)
• Cyr Y, Lamantia V, Bissonnette S et al. Lower plasma PCSK9 in normocholesterolemic subjects is associated with upregulated adipose tissue surface-expression of LDLR and CD36 and NLRP3 inflammasome Physiological reports 2021-02-01 [PMID: 33527668] (IHC-P, Human)
• Alajbegovic A, Holmberg J, Daoud F, et al. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells Vascular pharmacology 2021-01-28 [PMID: 33516965] (WB, Human)
• Srikanthan S McIntyre T M. ROLE OF EXOSOMES IN ATHEROTHROMBOTIC PROCESSES Atherosclerosis 2020-12-02 [PMID: 33261815]
• Dhanasekara CS, Zhang J, Nie S et al Nanoparticles target intimal macrophages in atherosclerotic lesions Nanomedicine 2020-12-01 [PMID: 33259961] (ELISA)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Ghaffari, S, Jang, E Et al. Endothelial HMGB1 Is a Critical Regulator of LDL Transcytosis via an SREBP2-SR-BI Axis. Arterioscler Thromb Vasc Biol 2021-01-01 [PMID: 33054399] (WB, Mouse)
• Navas Madronal M, Castelblanco E, Camacho M et al. Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis Preprint 2020-08-05 [PMID: 33028031] (WB, Human)
• Hsiao WY, Jung SM, Tang Y et al. The Lipid Handling Capacity of Subcutaneous Fat Is Programmed by mTORC2 during Development Cell Rep 2020-10-06 [PMID: 33027655] (WB, Mouse)
• Barroso, E, RodrIguez-RodrIguez, R Et al. SIRT3 deficiency exacerbates fatty liver by attenuating the HIF1 alpha -LIPIN 1 pathway and increasing CD36 through Nrf2. Cell Commun Signal 2020-09-10 [PMID: 32912335] (WB, Rat)
• Rey E, Del Pozo-Maroto E, MaraNOn P et al. Intrahepatic Expression of Fatty Acid Translocase CD36 Is Increased in Obstructive Sleep Apnea Front Med (Lausanne) 2020-08-11 [PMID: 32850919] (IHC-P, WB, Human)
• Lin YC, Wang JC, Wu MS et al. Nifedipine Exacerbates Lipogenesis in the Kidney via KIM-1, CD36, and SREBP Upregulation: Implications from an Animal Model for Human Study Int J Mol Sci 2020-06-19 [PMID: 32575412] (WB, Rat)
• Luo X, Li Y, Yang P et al. Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function EBioMedicine 2020-06-01 [PMID: 32516742] (ICC/IF, IHC-P, WB, Mouse)
• Liu Y, Munisso MC, Mahara A et al. Anti-platelet adhesion and in situ capture of circulating endothelial progenitor cells on ePTFE surface modified with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and hemocompatible peptide 1 (HCP-1) Colloids Surf B Biointerfaces 2020-05-15 [PMID: 32447201] (Porcine)
• Trivedi PC, Bartlett JJ, Mercer A et al. Loss of function of transcription factor EB remodels lipid metabolism and cell death pathways in the cardiomyocyte Biochim Biophys Acta Mol Basis Dis 2020-05-08 [PMID: 32437957] (WB, Mouse)
• Hu Zb, Lu J, Chen Pp et Al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis Theranostics 2020-02-03 [PMID: 32194836] (IF, IHC, WB, Rat)
• Jayakumari Nr, Rajendran Rs, Sivasailam A et Al. Impaired substrate-mediated cardiac mitochondrial complex I respiration with unaltered regulation of fatty acid metabolism and oxidative stress status in type 2 diabetic Asian Indians J Diabetes 2020-03-03 [PMID: 32125087] (WB, Human)
• Yang P, Zeng H, Tan W et al. Loss of CD36 impairs hepatic insulin signaling by enhancing the interaction of PTP1B with IR FASEB J. 2020-02-25 [PMID: 32100381] (WB, Mouse)
• Rawji KS, Young AMH, Ghosh T et al. Niacin-mediated rejuvenation of macrophage/microglia enhances remyelination of the aging central nervous system Acta Neuropathol. 2020-02-06 [PMID: 32030468] (IHC-Fr, Mouse)
• Feng WW, Wilkins O, Bang S et al. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies Cell Rep 2019-12-10 [PMID: 31825825]
• Lin HY, Wang FS, Yang YL et al. MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice Cells 2019-10-22 [PMID: 31652636] (WB, Mouse)
• Knackstedt S L, Georgiadou A et al. Neutrophil extracellular traps drive inflammatory pathogenesis in malaria. Sci Immunol 2019-10-18 [PMID: 31628160] (IHC, Mouse)
• Wilhelmsen AJ, Mallinson J, Jones R et al. Chronic effects of high intensity interval training on postprandial lipaemia in healthy males J. Appl. Physiol. 2019-10-17 [PMID: 31622161]
• Srisowanna N, Choijookhuu N, Yano K et al. The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats Acta Histochem Cytochem 2019-08-30 [PMID: 31592200] (IHC-P, Rat)
• Hurr C, Simonyan H, Morgan DA et al. Liver sympathetic denervation reverses obesity-induced hepatic steatosis J. Physiol. (Lond.) 2019-07-06 [PMID: 31278754]
• Ramalho T, Ramalingam L, Filgueiras L et al. Leukotriene-B4 modulates macrophage metabolism and fat loss in type 1 diabetic mice J. Leukoc. Biol. 2019-06-26 [PMID: 31242337] (WB, Mouse)
• Huang, L;Chambliss, KL;Gao, X;Yuhanna, IS;Behling-Kelly, E;Bergaya, S;Ahmed, M;Michaely, P;Luby-Phelps, K;Darehshouri, A;Xu, L;Fisher, EA;Ge, WP;Mineo, C;Shaul, PW; SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature 2019-04-24 [PMID: 31019307] (WB, Human)
• Lin, YC;Wu, MS;Lin, YF;Chen, CR;Chen, CY;Chen, CJ;Shen, CC;Chen, KC;Peng, CC; Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway Int J Mol Sci 2019-03-29 [PMID: 30934807] (WB, Rat)
• Yue H, Febbraio M, Klenotic PA et al. CD36 Enhances Vascular Smooth Muscle Cell Proliferation and Development of Neointimal Hyperplasia Arterioscler. Thromb. Vasc. Biol. 2018-12-20 [PMID: 30567481] (IHC, Mouse)
• Abbas A, Yu L et al. Development and Characterization of an 18F-labeled Ghrelin Peptidomimetic for Imaging the Cardiac Growth Hormone Secretagogue Receptor. Mol Imaging 2018-06-11 [PMID: 30394854] (ICC/IF, Mouse)
• Zhong S, Li L, Zhang YL et al. Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. J. Clin. Invest. 2018-10-30 [PMID: 30375985] (WB, Mouse)
• Lu Z, Li Y, Ru J et al. Interaction of palmitate and LPS regulates cytokine expression and apoptosis through sphingolipids in human retinal microvascular endothelial cells. Experimental Eye Research 2018-09-01 [PMID: 30273577] (WB, Human)
• Lindsey ML, Jung M, Yabluchanskiy A et al. Exogenous CXCL4 Infusion Inhibits Macrophage Phagocytosis by Limiting CD36 Signaling to Enhance Post-myocardial Infarction Cardiac Dilation and Mortality. Cardiovasc. Res. 2018-08-29 [PMID: 30169632] (WB, Human)
• Qian K, Feng L, Sun Y et al. Overexpression of Salusin-a Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model. BioMed Research International 2018-07-12 [PMID: 30105261] (WB, Rabbit)
• Fang Y, Wang J, Yao L et al. The adhesion and migration of microglia to B-amyloid (AB) is decreased with aging and inhibited by Nogo/NgR pathway. J Neuroinflammation. 2018-07-20 [PMID: 30029608] (WB, Mouse)
• Li Y, Lu Z, Ru JH et al. Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro. Am. J. Physiol. Endocrinol. Metab. 2018-07-10 [PMID: 29989851] (IHC-Fr, Mouse)
• Chen X, Austin ED, Talati M et al. Oestrogen inhibition reverses pulmonary arterial hypertension and associated metabolic defects Eur. Respir. J. 2017-08-01 [PMID: 28775043] (WB, Mouse)
• Gouveia V, Rizzello L, Vidal B et al. Targeting Macrophages and Synoviocytes Intracellular Milieu to Augment Anti Inflammatory Drug Potency Advanced Therapeutics 2022-01-10 (WB, Human)
• Sankar SB, Donegan RK, Shah KJ et al. Heme and hemoglobin suppress amyloid b-mediated inflammatory activation of mouse astrocytes J. Biol. Chem. 2018-06-05 [PMID: 29871926] (WB, Mouse)
• Misra A, Feng Z, Chandran RR et al. Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells Nat Commun 2018-05-25 [PMID: 29802249] (IHC-Fr, Mouse)
• Rozovski U, Harris DM, Li P et al. STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells Oncotarget 2018-04-20 [PMID: 29765537] (ICC/IF, Human)
• Lee JY, Han SH, Park MH et al. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease. Nat Commun 2018-04-16 [PMID: 29662056] (WB, Mouse)
• Guri Y, Colombi M, Dazert E et al. mTORC2 Promotes Tumorigenesis via Lipid Synthesis. Cancer Cell 2017-12-11 [PMID: 29232555] (Mouse)
• Xian X, Ding Y, Dieckmann M et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis Elife. 2017-11-16 [PMID: 29144234] (WB, Mouse)
• Sakamuri SSVP, Watts R, Takawale A et al. Absence of Tissue Inhibitor of Metalloproteinase-4 (TIMP4) ameliorates high fat diet-induced obesity in mice due to defective lipid absorption Sci Rep. 2017-07-23 [PMID: 28740132] (IHC-P, Mouse)
• Chen Y, Huang W, Yang M et al. Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase. Arterioscler. Thromb. Vasc. Biol. 2017-06-15 [PMID: 28619997] (Mouse)
• Delgado-Maroto Virginia, Benitez Raquel, Forte-Lago Irene et al. Cortistatin reduces atherosclerosis in hyperlipidemic ApoE-deficient mice and the formation of foam cells. Scientific Reports 2017-04-13 [PMID: 28406244] (WB, Mouse)
• Lavie L, Dyugovskaya L, Polyakov A et al. Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro. J Vis Exp. 2017-01-25 [PMID: 28190059]
• Garbacz WG, Lu P, Miller TM et al. Hepatic overexpression of CD36 improves glycogen homeostasis and attenuates high-fat diet induced hepatic steatosis and insulin resistance. Mol. Cell. Biol. 2016-08-15 [PMID: 27528620] (WB, Mouse)
• Vaillant F, Lauzier B, Ruiz M et al. Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia. Am. J. Physiol. Heart Circ. Physiol. 2016-10-01 [PMID: 27496881] (WB)
• Salameh A, Daquinag AC, Staquicini DI et al. Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue JCI Insight 2016-07-07 [PMID: 27468426]
• Sachdev V, Leopold C, Bauer R et al. Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism. Molecular and Cell Biology of Lipids. 2016-06-23 [PMID: 27344248]
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Souza AC, Bocharov AV, Baranova IN et al. Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation. Kidney Int. 2016-04-01 [PMID: 26994575] (IHC-P, Mouse)
• Chen X, Talati M et al. Estrogen Metabolite 16 alpha-Hydroxyestrone Exacerbates Bone Morphogenetic Protein Receptor Type II-Associated Pulmonary Arterial Hypertension Through MicroRNA-29-Mediated Modulation of Cellular Metabolism. Circulation 2016-05-01 [PMID: 26487756] (WB, Human, Mouse)
• Bartels ED, Christoffersen C, Lindholm MW, Nielsen LB. Altered Metabolism of LDL in the Arterial Wall Precedes Atherosclerosis Regression. Circ. Res. 2015-09-10 [PMID: 26358193] (IHC, Mouse)
• Zidek LM, Ackermann T, Hartleben G et al. Deficiency in mTORC1-controlled C/EBPbeta-mRNA translation improves metabolic health in mice. EMBO Rep 2015-08-01 [PMID: 26113365] (WB, Mouse)
• Barroso E, Rodriguez-Rodriguez R, Chacon MR et al. PPARbeta/delta ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation Biochim. Biophys. Acta 2015-05-01 [PMID: 25728706] (WB, Mouse)
• Konstantynowicz-Nowicka K, Harasim E, Baranowski M, Chabowski A. New evidence for the role of ceramide in the development of hepatic insulin resistance PLoS ONE. 2015-01-31 [PMID: 25635851] (WB, Rat)
  
  Details:
  CD36/SR-B3 (FAT) antibody used for WB on lysates of primary rat hepatocytes that were incubated with high glucose DMEM culture medium and were treated or not with 0.75 mM palmitate for 16 and 40 hours (Figure 5).
• Ye R, Holland Wl, Gordillo R et al. Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes B-cell regeneration. Elife. 2014-10-23 [PMID: 25339419] (IHC, Mouse)
• Srikanthan S, Li W, Silverstein RL, McIntyre TM. Exosome poly-ubiquitin inhibits platelet activation, downregulates CD36, and inhibits pro-atherothombotic cellular functions. J Thromb. Haemost. 2014-08-28 [PMID: 25163645]
• Chen Jingjing, Yong Yangyang, Xia Xian et al. The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36. Carbohydr Polym. 2014-11-04 [PMID: 25129711]
• Hale JS, Otvos B, Sinyuk M et al. Cancer stem cell-specific scavenger receptor CD36 drives glioblastoma progression. Stem Cells 2014-04-15 [PMID: 24737733] (IHC-P, Human)
• Khan S, Abu Jawdeh BG, Goel M et al. Lipotoxic disruption of NHE1 interaction with PI(4,5)P2 expedites proximal tubule apoptosis. J. Clin. Invest. 2014-03-04 [PMID: 24531551] (IHC-Fr, Mouse)
• Zhao Y, Xiong Z, Lechner EJ et al. Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury. Mucosal Immunol. 2013-09-18 [PMID: 24045574]
• Himoto T, Tani J, Miyoshi H et al. Investigation of the factors associated with circulating soluble CD36 levels in patients with HCV-related chronic liver disease. Diabetol Metab Syndr. 2013-09-09 [PMID: 24016701] (ICC/IF, IHC-P, Human)
• McGillicuddy FC, Reynolds CM, Finucane O, Coleman E et al. Long-term exposure to a high-fat diet results in the development of glucose intolerance and insulin resistance in interleukin-1 receptor I-deficient mice. Am J Physiol Endocrinol Metab. 2013-10-01 [PMID: 23921145] (WB, Mouse)
• Ledee D, Portman MA, Kajimoto M et al. Thyroid hormone reverses aging-induced myocardial Fatty Acid oxidation defects and improves the response to acutely increased afterload. PLoS One 2013-06-07 [PMID: 23762386] (WB, Mouse)
• Kajimoto M, O'Kelly Priddy CM, Ledee DR et al. Extracorporeal membrane oxygenation promotes long chain fatty acid oxidation in the immature swine heart in vivo. J Mol Cell Cardiol 2013-05-30 [PMID: 23727393] (WB, Porcine)
• Das R, Ganapathy S, Mahabeleshwar GH, Drumm C et al. Macrophage Gene Expression and Foam Cell Formation Are Regulated by Plasminogen. Circulation 2013-03-19 [PMID: 23401155] (WB, Mouse)
• Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013-02-19 [PMID: 23334894] (WB, Mouse)
• McLean BA, Kienesberger PC, Wang W et al. Enhanced recovery from ischemia-reperfusion injury in PI3Kalpha dominant negative hearts: Investigating the role of alternate PI3K isoforms, increased glucose oxidation and MAPK signaling. J Mol Cell Cardiol 2012-11-08 [PMID: 23142539] (WB, Mouse)
  
  Details:
  Using the HRP conjugated version of NB400-144, catalog number NB400-144H.
• Olson AK, Ledee D, Iwamoto K et al. C-Myc induced compensated cardiac hypertrophy increases free fatty acid utilization for the citric acid cycle J Mol Cell Cardiol 2012-07-22 [PMID: 22828478] (WB, Mouse)
• Pienimaeki-Roemer A, Ruebsaamen K, Boettcher A et al. Stored platelets alter glycerophospholipid and sphingolipid species, which are differentially transferred to newly released extracellular vesicles Transfusion 2012-07-15 [PMID: 22804622] (WB, Human)
• Faustino JV, Wang X, Johnson CE et al. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. J Neurosci;31(36):12992-13001. 2011-09-07 [PMID: 21900578]
• Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010-08-01 [PMID: 20629037]
• Lee JH, Wada T, Febbraio M et al. A novel role for the dioxin receptor in fatty acid metabolism and hepatic steatosis. Gastroenterology;139(2):653-63. 2010-08-01 [PMID: 20303349] (WB, Human, Mouse)
• Tagliani E, Guermonprez P, Sepulveda J et al. Selection of an antibody library identifies a pathway to induce immunity by targeting CD36 on steady-state CD8 alpha+ dendritic cells. J Immunol;180(5):3201-9. 2008-03-01 [PMID: 18292544] (WB, Mouse)
• Dynes J. The Effects of Chemotherapy Schedules on Ovarian Function. Thesis. 2014-12-01 (IHC-P, WB, Mouse)
  
  Details:
  CD36/SR-B3 antibody used for WB at 1:2000 dilution (Figure 9) and for IHC-P at 1:1000 dilution (Figure 18) in mice that were subjected to Low-Dose Metronomic (LDM) chemotherapy scheduling and traditional Maximum-Tolerated Dose (MTD) of cyclophosphamide.
• Naghibosadat M Characterization of the Growth Hormone Secretagogue Receptor in Dilated Cardiomyopathy Thesis 2019-06-20 (IHC, Mouse)
• Baines RJ, Chana RS, Hall M. CD36 mediates proximal tubular binding and uptake of albumin and is upregulated in proteinuric nephropathies Am J Physiol Renal Physiol 2012-10-01 [PMID: 22791331] (IHC, WB, Mouse)
• Cheng J, Krausz KW, Tanaka N, Gonzalez FJ. Chronic exposure to rifaximin causes hepatic steatosis in pregnane x receptor-humanized mice Toxicol Sci 2012-10-01 [PMID: 22790967] (WB, Mouse)
• Huang W, Febbraio M, Silverstein RL. CD9 Tetraspanin Interacts with CD36 on the Surface of Macrophages: A Possible Regulatory Influence on Uptake of Oxidized Low Density Lipoprotein. PLoS ONE 6(12). 2011-01-01 [PMID: 22216174]
• Kienesberger P, Pulinilkunnil T, Sung M et al. Myocardial ATGL overexpression decreases the reliance on fatty acid oxidation and protects against pressure overload-induced cardiac dysfunction. Mol Cell Biol 2012-01-01 [PMID: 22158969]
  
  Details:
  Using the HRP conjugated version of NB400-144, catalog number NB400-144H.
• Sos BC, Harris C, Nordstrom SM et al. Abrogation of growth hormone secretion rescues fatty liver in mice with hepatocyte-specific deletion of JAK2 J Clin Invest 2011-04-01 [PMID: 21364286] (WB, Mouse)
• Marecki JC, Ronis MJ, Shankar K, Badger TM. Hyperinsulinemia and ectopic fat deposition can develop in the face of hyperadiponectinemia in young obese rats. J Nutr Biochem;22(2):142-52. 2011-02-01 [PMID: 20435456] (WB, Rat)
• Hoppmann S, Steinbach J, Pietzsch J. Scavenger receptors are associated with cellular interactions of S100A12 in vitro and in vivo. Int J Biochem Cell Biol 2010-05-01 [PMID: 20025991] (WB, Human, Rat)
• Chabowski A, Zmijewska M, Gorski J et al. IL-6 deficiency increases fatty acid transporters and intramuscular lipid content in red but not white skeletal muscle. J Physiol Pharmacol;59 Suppl 7:105-17. 2008-12-01 [PMID: 19258660] (WB, Mouse)

Reviews for CD36 Antibody (NB400-144) (9)

Average Rating: 3.8

(Based on 9 reviews)

We have 9 reviews tested in 3 species: Mouse, Mouse and Rat, Other.

reviewed by: Verified Customer Simple Western Mouse and Rat 02/16/2022

Summary

• Application: Simple Western
• Sample Tested: Mouse adipose tissue,mouse liver,rat adipose tissue
• Species: Mouse and Rat
• Lot: N-1

Comments

• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.***
  Western blot was performed using the protein simple WES system
  A 12-230 kDa plate kit was used and the protocol was performed as stated in the protein simple WES user guide.
  mouse and rat tissues were homogenized with T-PER lysis buffer Thermo-Scientific 78510
  all samples were at a protein concentration of 0.4 mg/ml before loading them onto the plate.
  CD36 antibody concentrate was diluted 1:10 in order to use 100 ug/ml on all samples

reviewed by: Hongxue Shi ICC Mouse 10/02/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: Bone Marrrow Derived Macrophages
• Species: Mouse
• Lot: L-3

reviewed by: Verified Customer WB Mouse 03/13/2018

Summary

• Application: Western Blot
• Sample Tested: Primary muscle cell lysate
• Species: Mouse
• Lot: J-3

reviewed by: Wenliang Zhang WB Mouse 03/01/2018

Summary

• Application: Western Blot
• Sample Tested: white adipose tissue
• Species: Mouse
• Lot: f-5

reviewed by: Verified Customer IHC Mouse 05/11/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: mouse liver
• Species: Mouse

reviewed by: Kristine DeLeon-Pennell WB Mouse 11/30/2016

Summary

• Application: Western Blot
• Sample Tested: cardiac tissue
• Species: Mouse

Comments

• Comments: Used antibody at a 1:1000 dilution in insoluble fraction of infarct tissue. Antibody shows many bands especially in post-infarct tissue.

reviewed by: Verified Customer IF Mouse 11/30/2016

Summary

• Application: Immunofluorescence
• Sample Tested: cardiac tissue
• Species: Mouse

Comments

• Comments: Co-localization was performed using either an antibody for Mac-3 and CD36 to determine if macrophages were a major source of CD36. We used paraffin embedded infarct tissue.

reviewed by: cynthia morimoto WB Other 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23727393
• Species: Other

reviewed by: Verified Customer WB Mouse 02/09/2011

Summary

• Application: Western Blot
• Sample Tested: Whole tissue lysate ApoE murine aorta, Sample Amount: 20ug
• Species: Mouse
• Lot: C2

FAQs for CD36 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
   • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
   • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: CD36