CD36 Antibody - BSA Free

Images

 
Biological Strategies: Immunohistochemistry: CD36 Antibody [NB400-144] - Timp4-deficiency results in defective lipid digestion and absorption. (B) Immunostaining for CD36 in small intestine (proximal region) of ...read more
Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of CD36 in human adipocytes.
Western Blot: CD36 Antibody [NB400-144] - Mouse primary muscle cell lysate. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CD36 Antibody [NB400-144] - Bone marrrow derived mouse macrophages stained with CD36 antibody. ICC/IF image submitted by a verified customer review.
Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.
B) Representative immunoblotting bands and densitometric results of CD36 in liver tissue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31652636), licensed under a CC-BY ...read more
Niacin treatment of middle-aged mice alters macrophage/microglia morphology and promotes myelin phagocytosis. h) Representative images of lysolecithin lesions 7 days post-demyelination from middle-aged ...read more
Integrin beta3 modulates SMC transdifferentiation. a-c Mice were fed a HFD for 6 or 16 weeks as indicated, and then transverse aortic root sections were stained. In a, b sections from ApoE(-/-), SMMHC-CreERT2, ...read more
ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular ...read more
EpEX and EpCAM activate a STAT3-HIF2 alpha signal for EpEX/EpCAM-mediated iPSC formation.(A) iPSCs were infected with two different EpCAM shRNAs (two clones, #1 and #2). The protein expressions of EpCAM and HIF2 alpha ...read more
Western Blot: Rabbit Polyclonal CD36 Antibody [NB400-144] - The western blot was conducted on avian (passerine) skeletal muscle tissue homogenate. 6ug of total protein was loaded and GAPDH was probed to ensure total ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

CD36 Antibody - BSA Free Summary

Immunogen
This CD36 Antibody was developed against a synthetic peptide mapping to a region of human CD36 between residues 100-200 [Uniprot# P16671]
Localization
Integral membrane
Marker
Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD36
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551)
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Western Blot 1:500-1:2000
Application Notes
In Western Blot, a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD36 Overexpression Lysate
Human Adipose Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
CD36 Protein (NB400-144PEP)
Reviewed Applications
Read 10 Reviews rated 3.8
using
NB400-144 in the following applications:

Publications
Read Publications using
NB400-144 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34974159) Porcine reactivity reported in scientific literature (PMID: 23727393). Rat reactivity reported in scientific literature (PMID: 25635851). Rabbit reactivity reported in scientific literature (PMID: 30105261). Avian reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD36 Antibody - BSA Free

  • BDPLT10
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)
  • CD36 molecule (thrombospondin receptor)
  • CHDS7
  • cluster determinant 36
  • FAT
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3B
  • GP4
  • GPIIIB
  • GPIV
  • leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4 protein
  • PASIV
  • platelet glycoprotein 4
  • platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3

Background

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-144
Species: Hu, Mu, Rt, Po, Av, Bv, Pm, Rb
Applications: WB, ICC/IF, IHC

Publications for CD36 Antibody (NB400-144)(122)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Rabbit.

We have publications tested in 10 applications: B/N, ELISA, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-P, PLA, WB.


Filter By Application
B/N
(4)
ELISA
(1)
ICC/IF
(10)
IF
(1)
IF/IHC
(7)
IHC
(1)
IHC-Fr
(5)
IHC-P
(11)
PLA
(1)
WB
(70)
All Applications
Filter By Species
Human
(21)
Mouse
(53)
Rat
(11)
Bovine
(1)
Porcine
(2)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 122. Show All 122 Publications.
Publications using NB400-144 Applications Species
Hayashi KG, Sakumoto R Differential expression of pro- and anti-angiogenic factors in the endometrium between repeat breeder and normally fertile cows Animal Reproduction Science 2023-05-01 [PMID: 37270879] (IHC-P, Bovine) IHC-P Bovine
Tseng KY, Stratoulias V, Hu WF et al. Augmenting hematoma-scavenging capacity of innate immune cells by CDNF reduces brain injury and promotes functional recovery after intracerebral hemorrhage Cell death & disease 2023-02-15 [PMID: 36792604] (IHC-Fr, WB, Rat, Mouse) IHC-Fr, WB Rat, Mouse
Kim D, Jang G, Hwang J et al. Combined Therapy of Low-Dose Angiotensin Receptor-Neprilysin Inhibitor and Sodium-Glucose Cotransporter-2 Inhibitor Prevents Doxorubicin-Induced Cardiac Dysfunction in Rodent Model with Minimal Adverse Effects Pharmaceutics 2022-11-28 [PMID: 36559124] (WB, Mouse) WB Mouse
Gouveia V, Rizzello L, Vidal B et al. Targeting Macrophages and Synoviocytes Intracellular Milieu to Augment Anti Inflammatory Drug Potency Advanced Therapeutics 2022-01-10 (WB, Human) WB Human
Trivedi P ROLE OF TRANSCRIPTION FACTOR EB (TFEB) IN CARDIAC HEALTH AND DISEASE Thesis 2020-01-01 (WB, Rat) WB Rat
Naghibosadat M Characterization of the Growth Hormone Secretagogue Receptor in Dilated Cardiomyopathy Thesis 2019-06-20 (IF/IHC, Mouse) IF/IHC Mouse
Dynes J. The Effects of Chemotherapy Schedules on Ovarian Function. Thesis. 2014-12-01 (IHC-P, WB, Mouse)

Details:
CD36/SR-B3 antibody used for WB at 1:2000 dilution (Figure 9) and for IHC-P at 1:1000 dilution (Figure 18) in mice that were subjected to Low-Dose Metronomic (LDM) chemotherapy scheduling and traditional Maximum-Tolerated Dose (MTD) of cyclophosphamide.
IHC-P, WB Mouse
Ma Y, Huang L, Zhang Z et al. CD36 promotes tubular ferroptosis by regulating the ubiquitination of FSP1 in acute kidney injury Genes & Diseases 2023-01-01
Ma Y, Huang L, Zhang Z et al. CD36 promotes tubular ferroptosis by regulating the ubiquitination of FSP1 in acute kidney injury Genes & Diseases 2024-01-01 [PMID: 37588197] (ICC/IF, IHC) ICC/IF, IHC
Boso D, Tognon M, Curtarello M et al. Anti-VEGF therapy selects for clones resistant to glucose starvation in ovarian cancer xenografts Journal of Experimental & Clinical Cancer Research 2023-08-07 [PMID: 37550722] (B/N, WB) B/N, WB
Show All 122 Publications.

Reviews for CD36 Antibody (NB400-144) (10) 3.810

Average Rating: 3.8
(Based on 10 reviews)
We have 10 reviews tested in 4 species: Mouse, Avian and Passerine, Mouse and Rat, Other.

Reviews using NB400-144:
Filter by Applications
WB
(6)
Simple Western
(1)
ICC
(1)
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(7)
Avian and Passerine
(1)
Mouse and Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CD36 NB400-144
Enlarge
4
reviewed by:
Sampath Kumar Anandan
WB Avian and Passerine 02/23/2024
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesAvian and Passerine
LotD147570

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
The western blot was conducted on avian (passerine) skeletal muscle tissue homogenate. 6ug of total protein was loaded and GAPDH was probed to ensure total protein loads were similar across samples. Novus biologicals Rabbit polyclonal CD36 antibody (NB400-144) was used at 1:5000 dilution (diluted in 5% milk). CD36 and GAPDH were probed sequentially - not multiplexed. CD36 produced a band above 40kDa and GAPDH at 36kDa.
  1
reviewed by:
Erin Giles
Simple Western Mouse and Rat 02/16/2022
View

Summary

ApplicationSimple Western
Sample TestedMouse adipose tissue,mouse liver,rat adipose tissue
SpeciesMouse and Rat
LotN-1

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Western blot was performed using the protein simple WES system
A 12-230 kDa plate kit was used and the protocol was performed as stated in the protein simple WES user guide.
mouse and rat tissues were homogenized with T-PER lysis buffer Thermo-Scientific 78510
all samples were at a protein concentration of 0.4 mg/ml before loading them onto the plate.
CD36 antibody concentrate was diluted 1:10 in order to use 100 ug/ml on all samples
Immunocytochemistry CD36 NB400-144
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3
reviewed by:
Verified Customer
ICC Mouse 10/02/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
LotL-3
Western Blot CD36 NB400-144
Enlarge
5
reviewed by:
Marie-Claude Sincennes
WB Mouse 03/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedPrimary muscle cell lysate
SpeciesMouse
LotJ-3
Western Blot CD36 NB400-144
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 03/01/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhite adipose tissue
SpeciesMouse
Lotf-5
Immunohistochemistry CD36 NB400-144
Enlarge
4
reviewed by:
Chansol Hurr
IHC Mouse 05/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver
SpeciesMouse
  3
reviewed by:
Verified Customer
WB Mouse 11/30/2016
View

Summary

ApplicationWestern Blot
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsUsed antibody at a 1:1000 dilution in insoluble fraction of infarct tissue. Antibody shows many bands especially in post-infarct tissue.
Immunofluorescence CD36 NB400-144
Enlarge
4
reviewed by:
Kristine DeLeon-Pennell
IF Mouse 11/30/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsCo-localization was performed using either an antibody for Mac-3 and CD36 to determine if macrophages were a major source of CD36. We used paraffin embedded infarct tissue.
  4
reviewed by:
Verified Customer
WB Other 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23727393
SpeciesOther
Western Blot CD36 NB400-144
Enlarge
5
reviewed by:
Cathal McCarthy
WB Mouse 02/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedWhole tissue lysate ApoE murine aorta, Sample Amount: 20ug
SpeciesMouse
LotC2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD36 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
    • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
  2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
    • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for CD36 Antibody (NB400-144)

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Pathways for CD36 Antibody (NB400-144)

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Research Areas for CD36 Antibody (NB400-144)

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Blogs on CD36.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

How do Lipase A and the CD36 Antibody Relate to Each Other
Obesity, diabetes and metabolic disorders are dramatically on the increase, linked to disorders such as heart disease, stroke and cancer. To combat this, research groups are studying metabolism at both a cellular and a systemic level. Although we at N...  Read full blog post.

ABCA1 Mutations: A Risk Factor in Atherosclerosis Related Strokes
The ATP Binding Cassette Transporter (ABCA1) gene encodes the cholesterol regulatory efflux protein, which plays a key role in lipid metabolism. ABCA1 antibody products are an important part of any antibody catalog covering atherosclerosis disease res...  Read full blog post.

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Recent Reviews

3.8
5
3
4
4
3
2
2
0
1
1

Sampath Kumar Anandan
02/23/2024
Application: WB
Species: Avian and Passerine

Erin Giles
02/16/2022
Application: Simple Western
Species: Mouse and Rat

Verified Customer
10/02/2020
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CD36