CD36 Antibody

Images

 
Biological Strategies: Immunohistochemistry: CD36 Antibody [NB400-144] - Timp4-deficiency results in defective lipid digestion and absorption. (B) Immunostaining for CD36 in small intestine (proximal region) of ...read more
Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of CD36 in human adipocytes.
Western Blot: CD36 Antibody [NB400-144] - Mouse primary muscle cell lysate. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CD36 Antibody [NB400-144] - Bone marrrow derived mouse macrophages stained with CD36 antibody. ICC/IF image submitted by a verified customer review.
Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CD36 Antibody Summary

Immunogen
This CD36 Antibody was developed against a synthetic peptide mapping to a region of human CD36 between residues 100-200 [Uniprot# P16671]
Localization
Integral membrane
Marker
Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD36
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Western Blot 1:500-1:2000
Application Notes
This CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24531551). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD36 Overexpression Lysate
Human Adipose Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
CD36 Protein (NB400-144PEP)
Reviewed Applications
Read 8 Reviews rated 4.1
using
NB400-144 in the following applications:

Publications
Read Publications using
NB400-144 in the following applications:

  • 1 publication
  • 5 publications
  • IF
    1 publication
  • IHC
    7 publications
  • 4 publications
  • 9 publications
  • IP
    1 publication
  • WB
    53 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 23727393). Rat reactivity reported in scientific literature (PMID: 25635851). Rabbit reactivity reported in scientific literature (PMID: 30105261).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD36 Antibody

  • BDPLT10
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)
  • CD36 molecule (thrombospondin receptor)
  • CHDS7
  • cluster determinant 36
  • FAT
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3B
  • GP4
  • GPIIIB
  • GPIV
  • leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4 protein
  • PASIV
  • platelet glycoprotein 4
  • platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3

Background

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD36 Antibody (NB400-144)(88)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Rabbit.

We have publications tested in 8 applications: ELISA, ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(5)
IF
(1)
IHC
(7)
IHC-Fr
(4)
IHC-P
(9)
IP
(1)
WB
(53)
All Applications
Filter By Species
Human
(17)
Mouse
(47)
Rat
(8)
Porcine
(2)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 88. Show All 88 Publications.
Publications using NB400-144 Applications Species
Wang J, Qin X, Sun H Et al. Nogo receptor impairs the clearance of fibril amyloid-beta by microglia and accelerates Alzheimer's-like disease progression Aging cell Nov 24 2021 [PMID: 34821024] (WB, Mouse) WB Mouse
Jabbari K, Winkelmaier G, Andersen C et al. Protein Ligands in the Secretome of CD36+ Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines Cancers Sep 8 2021 [PMID: 34572749] (ICC/IF, Human) ICC/IF Human
Daquinag Ac, Gao Z, Fussell C Et Al. Fatty acid mobilization from adipose tissue is mediated by CD36 post-translational modifications and intracellular trafficking JCI insight Jul 27 2021 [PMID: 34314388] (WB) WB
Fujimoto BA, Young M, Nakamura N et al. Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model The Journal of biological chemistry Feb 26 2021 [PMID: 33647317] (WB, Mouse) WB Mouse
Jayakumari NR, Rajendran RS, Sivasailam A, et al. Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart European journal of pharmacology Jan 30 2021 [PMID: 33529726] (WB) WB
Cyr Y, Lamantia V, Bissonnette S et al. Lower plasma PCSK9 in normocholesterolemic subjects is associated with upregulated adipose tissue surface-expression of LDLR and CD36 and NLRP3 inflammasome Physiological reports Feb 1 2021 [PMID: 33527668] (IHC-P, Human) IHC-P Human
Alajbegovic A, Holmberg J, Daoud F, et al. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells Vascular pharmacology Jan 28 2021 [PMID: 33516965] (WB, Human) WB Human
Dhanasekara CS, Zhang J, Nie S et al Nanoparticles target intimal macrophages in atherosclerotic lesions Nanomedicine Dec 1 2020 [PMID: 33259961] (ELISA)

Details:
Citation using the Alexa Fluor 647 version of this antibody.
ELISA
Navas Madronal M, Castelblanco E, Camacho M et al. Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis Preprint Aug 5 2020 [PMID: 33028031] (WB, Human) WB Human
Hsiao WY, Jung SM, Tang Y et al. The Lipid Handling Capacity of Subcutaneous Fat Is Programmed by mTORC2 during Development Cell Rep Oct 6 2020 [PMID: 33027655] (WB, Mouse) WB Mouse
Show All 88 Publications.

Reviews for CD36 Antibody (NB400-144) (8) 4.18

Average Rating: 4.1
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Mouse, Other.

Reviews using NB400-144:
Filter by Applications
ICC
(1)
WB
(5)
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(7)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CD36 NB400-144
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3
reviewed by:
Verified Customer
ICC Mouse 10/02/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
LotL-3
Western Blot CD36 NB400-144
Enlarge
5
reviewed by:
Marie-Claude Sincennes
WB Mouse 03/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedPrimary muscle cell lysate
SpeciesMouse
LotJ-3
Western Blot CD36 NB400-144
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5
reviewed by:
Verified Customer
WB Mouse 03/01/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhite adipose tissue
SpeciesMouse
Lotf-5
Immunohistochemistry CD36 NB400-144
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4
reviewed by:
Chansol Hurr
IHC Mouse 05/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver
SpeciesMouse
  3
reviewed by:
Verified Customer
WB Mouse 11/30/2016
View

Summary

ApplicationWestern Blot
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsUsed antibody at a 1:1000 dilution in insoluble fraction of infarct tissue. Antibody shows many bands especially in post-infarct tissue.
Immunofluorescence CD36 NB400-144
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4
reviewed by:
Kristine DeLeon-Pennell
IF Mouse 11/30/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsCo-localization was performed using either an antibody for Mac-3 and CD36 to determine if macrophages were a major source of CD36. We used paraffin embedded infarct tissue.
  4
reviewed by:
Verified Customer
WB Other 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23727393
SpeciesOther

Blocking

Blocking DetailsSee PMID 23727393

Details

Detection NotesSee PMID 23727393

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23727393.
Western Blot CD36 NB400-144
Enlarge
5
reviewed by:
Cathal McCarthy
WB Mouse 02/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedWhole tissue lysate ApoE murine aorta, Sample Amount: 20ug
SpeciesMouse
LotC2
CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit , Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#sc-2004 Santa Cruz

Details

Detection NotesDetection Method: Supersignal Pierce, Exposure Time: 10 min, Specific Bands: 75kDa, Non-Specific Bands: None

Comments

CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Product General Protocols

View specific protocols for CD36 Antibody (NB400-144): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD36 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
    • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
  2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
    • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-144AF350
Alexa Fluor 405 NB400-144AF405
Alexa Fluor 488 NB400-144AF488
Alexa Fluor 532 NB400-144AF532
Alexa Fluor 594 NB400-144AF594
Alexa Fluor 647 NB400-144AF647
Alexa Fluor 700 NB400-144AF700
Alexa Fluor 750 NB400-144AF750
Biotin NB400-144B
DyLight 350 NB400-144UV
DyLight 405 NB400-144V
DyLight 488 NB400-144G
DyLight 550 NB400-144R
DyLight 594 NB400-144DL594
DyLight 650 NB400-144C
DyLight 680 NB400-144FR
DyLight 755 NB400-144IR
FITC NB400-144F
HRP NB400-144H
Janelia Fluor 549 NB400-144JF549
Janelia Fluor 646 NB400-144JF646

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Bioinformatics Tool for CD36 Antibody (NB400-144)

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Blogs on CD36.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

How do Lipase A and the CD36 Antibody Relate to Each Other
Obesity, diabetes and metabolic disorders are dramatically on the increase, linked to disorders such as heart disease, stroke and cancer. To combat this, research groups are studying metabolism at both a cellular and a systemic level. Although we at N...  Read full blog post.

ABCA1 Mutations: A Risk Factor in Atherosclerosis Related Strokes
The ATP Binding Cassette Transporter (ABCA1) gene encodes the cholesterol regulatory efflux protein, which plays a key role in lipid metabolism. ABCA1 antibody products are an important part of any antibody catalog covering atherosclerosis disease res...  Read full blog post.

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Recent Reviews

4.1
5
3
4
3
3
2
2
0
1
0

Verified Customer
10/02/2020
Application: ICC
Species: Mouse

Marie-Claude Sincennes
03/13/2018
Application: WB
Species: Mouse

Verified Customer
03/01/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CD36