Apolipoprotein A-I/ApoA1 Antibody

Images

 
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody ...read more
Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Apolipoprotein A-I/ApoA1 Antibody Summary

Immunogen
Partial recombinant human ApoA1 protein (amino acids 25-267) [UniProt P02647]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml - 1.0 ug/ml
  • Flow Cytometry 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:400 - 1:1000
  • Immunohistochemistry-Paraffin 1:400 - 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52979AF350
Alexa Fluor 405 NBP2-52979AF405
Alexa Fluor 488 NBP2-52979AF488
Alexa Fluor 532 NBP2-52979AF532
Alexa Fluor 594 NBP2-52979AF594
Alexa Fluor 647 NBP2-52979AF647
Alexa Fluor 700 NBP2-52979AF700
Alexa Fluor 750 NBP2-52979AF750
Biotin NBP2-52979B
DyLight 350 NBP2-52979UV
DyLight 405 NBP2-52979V
DyLight 488 NBP2-52979G
DyLight 550 NBP2-52979R
DyLight 594 NBP2-52979DL594
DyLight 650 NBP2-52979C
DyLight 680 NBP2-52979FR
DyLight 755 NBP2-52979IR
FITC NBP2-52979F
HRP NBP2-52979H
Janelia Fluor 549 NBP2-52979JF549
Janelia Fluor 646 NBP2-52979JF646

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Diseases for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

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Bioinformatics

Gene Symbol APOA1
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