Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Images

 
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Western blot analysis using Apolipoprotein A-I/ApoA1 antibody. Harvested from mouse, separated on a 4-12 gradient gel by SDS-PAGE, transferred to PVDF ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody ...read more
Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells ...read more
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Apolipoprotein A-I/ApoA1 Antibody - BSA Free Summary

Immunogen
Partial recombinant human ApoA1 protein (amino acids 25-267) [UniProt P02647]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:400 - 1:1000
  • Immunohistochemistry-Paraffin 1:400 - 1:1000
  • Western Blot 0.5 ug/ml - 1.0 ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP2-52979 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-75410
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB400-105
Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
NB600-610
Species: Bv, Hu
Applications: ELISA, IHC, IHC-P, IP, WB
NB300-558
Species: Bv, Hu, Rb
Applications: ELISA, ICC/IF, IP, WB
NBP1-05950
Species: Hu, Mu
Applications: ICC/IF, WB
NBP2-37477
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NBP2-52932
Species: Hu
Applications: IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
NB200-527
Species: Hu, Mu, Rt
Applications: IP, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
NB400-104
Species: ChHa, SyHa, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PLA, Simple Western, WB
AF2009
Species: Hu
Applications: ICC, IHC
AF7895
Species: Hu
Applications: IHC, WB

Publications for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979) (0)

There are no publications for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-52979:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Apolipoprotein A-I/ApoA1 NBP2-52979
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 03/17/2022
View

Summary

ApplicationWestern Blot
Sample TestedBlood plasma,peritoneal lavage
SpeciesMouse
LotA-1

Comments

CommentsThe antibody is easy to use and captures the blot very well!
WB dilution 1: 8000, block before incubation overnight

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Apolipoprotein A-I/ApoA1 Products

Bioinformatics Tool for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

Discover related pathways, diseases and genes to Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

Discover more about diseases related to Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979).
 

Pathways for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

View related products by pathway.

PTMs for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

Learn more about PTMs related to Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979).
 

Research Areas for Apolipoprotein A-I/ApoA1 Antibody (NBP2-52979)

Find related products by research area.

Blogs on Apolipoprotein A-I/ApoA1

There are no specific blogs for Apolipoprotein A-I/ApoA1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
03/17/2022
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol APOA1