RFC1 Antibody [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

RFC1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RFC1
Met800-Ser1093
Accession # P35251
Specificity
Detects human RFC1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
RFC1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RFC1 Antibody [Unconjugated]

  • A1 140 kDa subunit
  • A1
  • Activator 1 140 kDa subunit
  • Activator 1 large subunit
  • Activator 1 subunit 1
  • MGC51786
  • MHC binding factor, beta
  • MHCBFB
  • PO-GA
  • RECC1
  • replication factor C (activator 1) 1 (145kD)
  • replication factor C (activator 1) 1, 145kDa
  • Replication factor C 140 kDa subunit
  • Replication factor C large subunit
  • replication factor C subunit 1
  • replication factor C1
  • RF-C 140 kDa subunit
  • RFC
  • RFC1
  • RFC140
  • RFC140DNA-binding protein PO-GA

Background

RFC1 (Replication factor C subunit 1; also RFC140) is a 140‑150 kDa, ubiquitously expressed member of the activator 1 large subunit family of proteins. It is one of five RFC complex subunits that participate in the early stages of DNA replication. Following the initiation of DNA synthesis by pol alpha /primase, an RFC complex displaces pol alpha at the replication fork, and recruits a PCNA homotrimeric complex to this site. This interacts with polδ to extend the newly‑formed DNA strand. Human RFC1 is 1148 amino acids (aa) in length. It contains a phosphorylated-DNA binding region (aa 366‑477), a PCNA binding domain (aa 478‑712), an ATPase site (aa 643‑772), and an NLS (aa 1120‑1124). Caspase-3 cleaves RFC-1 into 97, 73 and 65 kDa fragments during apoptosis. There is one potential alternative start site at Met117. Over aa 800‑1093, human RFC1 shares 90% aa identity with mouse RFC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RFC1
Uniprot