| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-131SS | |||
| Azide and BSA Free | NBP2-80762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27409 | |||
| BSA Free | NB100-56704SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-42761 | |||
| HRP | NBP1-42762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-683-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56509SS | |||
| Azide and BSA Free | NBP2-80788 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-562SS | |||
| Azide and BSA Free | NBP2-80641 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64796SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80652-0.1mg | |||
| BSA Free | NB100-77417SS | |||
| Azide and BSA Free | NBP2-80652 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2219SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33337SS | |||
| Azide and BSA Free | NBP2-80656 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40766SS | |||
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Immunoblot validation of HIF2A and PHD2 KO clones using HIF2A (#NB100-122; dilution: 1/300), PHD2 (#NB100-137; dilution: 1/500). To blot HIFs factor cells were first pre-treated for 5 h with CoCl2 300 uM before protein extraction, a condition that promotes HIF factor accumulation. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06988-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Knockout-Validated-NB100-137-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Immunoblot validation of HIF2A and PHD2 KO clones using HIF2A (#NB100-122; dilution: 1/300), PHD2 (#NB100-137; dilution: 1/500). To blot HIFs factor cells were first pre-treated for 5 h with CoCl2 300 uM before protein extraction, a condition that promotes HIF factor accumulation. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06988-3) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Simple Western: EGLN1/PHD2 Antibody [NB100-137] - Simple Western lane view shows a specific band for PHD2/HIF Prolyl Hydroxylase 2 in 0.5 mg/mL of hypoxic HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Simple-Western-NB100-137-img0010.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 expression in human prostate cancer cell lines. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-137-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-137-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 in human glioblastoma cell lysates. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-137-img0005.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of human PHD2 by Western blot. Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. EGLN1/PHD2 antibody used at 1 ug/mL. Detection by chemiluminescence.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-137-img0013.jpg)
![Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - EGLN1/PHD2 and EGFR expression levels positively correlate in breast cancer. Processed tissue microarrays of breast cancer biopsies from 313 patients were stained with EGLN1/PHD2 and EGFR antibodies. Four representative immunohistochemistries of human breast cancer with low and high expression of EGLN1/PHD2 and EGFR are shown. Magnification 10x. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/14241/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-NB100-137-img0018.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Exogenous PHD2 has no impact on the gene expression of HIF-1alpha and HIF-2alpha. U87MG cells were transfected with empty vector (pcDNA) or PHD2 plasmid. Twenty-four hours after transfection, PHD2 was detected by immunoblotting. beta-Actin was used as loading control. The results are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014295), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-137-img0017.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - An intracellular stain was performed on Jurkat cells with NB100-137AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Flow-Cytometry-NB100-137-img0014.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in normoxia (lane 1) or hypoxia (lane 2) treated SK-N-BE(2) cells using anti-EGLN1/PHD2 antibody. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-137-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of PHD2 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-137-img0003.jpg)
![Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - Staining of lung vascular endothelium with EGLN1/PHD2 antibody. Image at 40X.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-NB100-137-img0015.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 in Jurkat cells. One million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/mL anti-EGLN1/PHD2 antibody in a 150 uL reaction. Isotype control (black), anti-MLL1 (red).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Flow-Cytometry-NB100-137-img0007.jpg)
![Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-210202423454817.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Representative picture of western blot in histopathologically unchanged tissue (N) & primary cancerous tissue (C) from patients with CRC. Immunodetection of bands was performed with Rp anti- PHD1, - PHD2, - PHD3 & - FIH Ab, followed by incubation with goat anti-rabbit HRP-conjugated Ab. The membrane was stripped & incubated with Rp anti-GAPDH Ab, followed by incubation with goat anti-rabbit HRP-conjugated Ab. Bands were revealed using SuperSignal West Femto Chemiluminescent Substrate, Thermo Fisher Scientific (Rockford, IL) & Biospectrum® Imaging System 500, UVP Ltd. (Upland, CA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24195777), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-310202415293310.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-31020241683931.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Oxygen chemotaxis is independent of the PHD/HIF pathway. a–dHIF1A, HIF2A, HIF1A+HIF2A & PHD2 CRISPR/Cas9 KO clones characterisation, respectively, regarding O2-directed migration. Left panels: immunoblot validation of HIF1A, HIF2A, HIF1A+HIF2A & PHD2 KO clones. To blot HIFs factors (a, b, c), cells were first pre-treated for 5 h with CoCl2 300 µM before protein extraction, a condition that promotes HIF factors accumulation (cf. Supplementary Fig. 8f). Middle panels: cell trajectories under confinement. Red dashed lines indicate the border of the cell cluster at 0 h. Right panels: relative distribution of MCF10A KO clones versus wt cells at the edge of the cluster at 48 h. These experiments demonstrate that HIF factors deletion does not prevent aerotaxis. e, f Tracks & redistribution of wt & PHD2 KO clone silenced for PHD3 (siPHD3) or not (siCTR). g Tracks & redistribution of MCF10A cells treated with DMOG (50 µM) or CoCl2 (50 µM) to inhibit PHDs, or with vehicle only (DMSO). These experiments demonstrate that PHDs do not participate in O2-sensing during aerotaxis. Confinement was applied for 48 h (a–g). Scale bars, 500 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30382089), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-31020241612618.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - PHD3 expression correlates with a mesenchymal-like morphology in pancreatic ductal adenocarcinoma cell lines.NHF-1 (Fibroblast) MiaPaca2, Panc1, CAPAN1 & BxPC3 cells were harvested for RNA & protein following 24 hours exposure to normoxia (21% O2) or hypoxia (1% O2). (A) Phase-contrast images at 10× magnification were taken of MiaPaca2 (mesenchymal-like) morphology & BxPC3 cells (differentiated, epithelial morphology) under normoxic conditions. (B) PHD3 mRNA expression was determined by qRT-PCR & graphed relative to BxPC3 in normoxia. All samples were normalized to 18S rRNA & graphed as expression relative to BxPC3-Vec Normoxia (lane 1). n = 3, error bars = 1 S.D. (C) Whole cell lysate was resolved by SDS-PAGE & blotted for beta -tubulin PHD3 & PHD2. N = normoxia, H = hypoxia (1% O2). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0083021), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-310202415541942.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Iron supplementation restores HIF-1 alpha levels to normal following ATP6V0E1 inhibition in HeLa cells. (A) Schematic diagram of the multimeric V-ATPase complex. (B) Chemical inhibition of V-ATPase by 10 nM BafA treatment for 24 h, increased HIF-1 alpha levels in HIF alpha -GFPODD reporter cells. Treatment with 100 μM Fe (III) citrate significantly reduced the elevated HIF-1 alpha levels associated with loss of ATP6V0E1 (1 × 106 cells per sample harvested & analyzed; N = 2). (C) Knock-down of ATP6V0E1 subunit with three different CRISPR-Cas9 guides resulted in significant upregulation of HIF-1 alpha levels in HIF alpha -GFPODD reporter cells. Co-treating cells with 100 μM Fe (III) citrate led to a reduction in HIF-1 alpha levels across the three ATP6V0E1 depleted cells. phd2 was knocked down as a control & treatment with 100 μM Fe (III) citrate did not result in reduction of HIF-1 alpha levels (1 × 106 cells per sample harvested & analyzed; N = 2). FACs plot shown is a representative image of two biological repeats performed. (D) Immunoblot analysis for HIF-1 alpha & PHD2 levels in HIF alpha -GFPODD reporter cells with either ATP6V0E1 or PHD2 depleted or treated with 10 nM BafA. The cells were treated with 100 μM Fe (III) citrate for 24 h. beta actin was used as a control. Results validated findings observed by flow cytometry, whereby HIF-1 alpha levels were upregulated following ATP6V0E1 knock-down or inhibition & levels were re-normalized upon Fe (III) citrate treatment. Treatment of Fe (III) citrate in PHD2 depleted cells did not alter HIF-1 alpha levels. All experiments were performed in biological duplicate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32984302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-310202415533838.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-137] - PHD3 depletion stabilizes hypoxic p27 expression by increasing p27 half-life. a Cell cycle arrest at G0 & subsequent release shows an increase of p27 expression in siPHD3 exposed cells. b Quantification for p27 expression under PHD3 depletion at indicated time points after cell cycle release in HeLa & 786-O cells. Asterisk indicates significant difference (p < 0,05; n = 3). c Cell cycle arrest at G0 & inhibition of protein synthesis with cycloheximide indicate increased p27 stability in PHD3 depleted HeLa cells. d Quantification of p27 expression using siPHD3 or control at indicated time points. Four independent experiments (± SEM) are shown (p < 0,05; n = 4). e Analysis of p27 stability in 786–0 cells by cycloheximide chase during reoxygenation after 24 h hypoxia demonstrates markedly increased half-life of p27 upon PHD3 depletion Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26223520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-137_rabbit-polyclonal-egln1-phd2-antibody-310202415533825.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0030.jpg "Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license.")
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Histologically distinct cell types in hemangioblastomas do not arise from a common ancestral clone. Representative images of sample SH-0622 acquired at 400x of (a) H + E and IHC for (b) HIF1-alpha reveal heterogenous cell types in this tumor characterized by a rich vascular network. Arrowheads indicate that the stromal cells demonstrate increased cytoplasmic staining for HIF1-alpha and VEGF, whereas the double arrowheads highlight PDGFR-beta protein restricted to vascular endothelium. Scale bar is 25 um. Image collected and cropped by CiteAb from the following publication (//www.actaneurocomms.org/content/2/1/167), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0029.jpg)
![Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Image shows a specific band for HIF-1 alpha in 0.5 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Simple-Western-NB100-131-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. 100X magnification. Blue: DAPI nuclear staining.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0016.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of a FFPE mouse kidney tissue section using HIF-1 alpha antibody clone ESEE122 at 1ug/mL concentration. The detection was performed using X-cell plus universal HRP polymer detection system with Vector SG chromagen substrate. Image courtesy of a product review by Steven Grover.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0019.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha in normal renal tissue (A) and clear cell renal cell carcinoma (CCRCC) (D). A homogeneous cytoplasmic staining of tubular cells and weak staining in glomerules was observed with HIF-1 alpha (A). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A and VEFG-C (E and F). (magnification x200). Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/28/1/40), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0031.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0021.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Nuclear HIF-1 alpha protein expression. Xenografts were established in female nude mice (BALB c[nu/nu]) by subcutaneous injection of head and neck squamous cell carcinoma cell lines UT-SCC-2 (UT-2) and UT-SCC-14 (UT-14). Cetuximab (1 mg/injection) or PBS was administered by intraperitoneal injection at day 10, 14, and 17. A tissue microarray was constructed from tumours harvested at day 21, and the expression of nuclear HIF-1 alpha was evaluated by immunohistochemistry (IHC) in untreated controls and cetuximab-treated tumour specimens. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28756482/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus, using NB100-131. Blue: DAPI nuclear staining. Image courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. 100X magnification. Image courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0007.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0018.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of a FFPE tissue section of human renal cancer xenograft using HIF-1 alpha antibody (NB100-131 Lot 83115) at 1:200 dilution. The antibody generated a strong cytoplasmic staining mainly in the cancer cells. Only a fraction of cells depicted nuclear staining, while weak to negligible positivity was seen in the tumor stromal cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0024.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - HIF-1 alpha staining in hypoxia-induced human placenta.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0025.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1alpha, c-Met, CA9 and GLUT1. HIF-1alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), and strong staining intensity in squamous cell carcinoma (C). Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/11/1/185), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0032.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Histologically distinct cell types in hemangioblastomas do not arise from a common ancestral clone. “Representative images of sample SH-0622 acquired at 400x of (a) H + E & IHC for (b) HIF1-alpha , (c) VEGF, & (d) PDGFR-beta reveal heterogenous cell types in this tumor characterized by a rich vascular network. Arrowheads indicate that the stromal cells demonstrate increased cytoplasmic staining for HIF1-alpha & VEGF, whereas the double arrowheads highlight PDGFR-beta protein restricted to vascular endothelium. Scale bar is 25 μm. Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-014-0167-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241534345.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical analysis in non-GIST STS representing negative, & score 1–3 of CAIX, GLUT-1, HIF-1 alpha , & HIF-2 alpha . non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, CAIX: carbonic anhydrase IX, GLUT-1: glucose transporter-1, & HIF-1/2 alpha : hypoxia induced factor 1/2 alpha . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22454562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415371952.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Digital image analysis of cytoplasmic & membranous staining. Cytoplasmic HIF-1 alpha staining is shown (A) & automated image analysis utilizing TissueIA recognizes cytoplasmic HIF-1 alpha staining highlighted in green color (B). CA9 is shown in membranous staining (C) & automated image analysis determines membranous CA9 staining highlighted in green color (D). The output from the algorithm returns a number of quantitative measurements for intensity & percentage of positive staining present. Scale bar: 100 μm. Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241534362.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha , VEGF-A & VEGF-C in normal renal tissue (A-C) & clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells & weak staining in glomerules was observed with HIF-1 alpha (A), while VEGF-A & VEGF-C were positive in tubular cells, glomerular mesangium & interstitial macrophages (B & C). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A & VEFG-C (E & F). (magnification ×200). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19302703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415371953.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Positive immunohistochemical staining for (A) VEGF, (B) HIF-1 alpha , (C) Dll4 (tumor cells), (D) Dll4 (endothelial cells), & (E) CD31 (for microvessel counting, ×200 magnification). Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/or.2012.2075), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415384140.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha , VEGF-A & VEGF-C in normal renal tissue (A-C) & clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells & weak staining in glomerules was observed with HIF-1 alpha (A), while VEGF-A & VEGF-C were positive in tubular cells, glomerular mesangium & interstitial macrophages (B & C). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A & VEFG-C (E & F). (magnification ×200). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19302703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415363744.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1 alpha , c-Met, CA9 & GLUT1. HIF-1 alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), & strong staining intensity in squamous cell carcinoma (C). c-Met (D-F), CA9 (G, H) & GLUT1 (I) shows cell membranous staining. Representative c-Met expression in cervical samples shown with no staining in normal cervix (D), weak membranous staining intensity in squamous cell carcinoma (E) & strong intensity in squamous cell carcinoma (F). CA9 expression showing moderate intensity staining in carcinoma in situ (CIS) (G) & strong staining in adenocarcinoma (H). GLUT1 expression showing strong intensity in squamous cell carcinoma (I). Scale bar: 50 μm. Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415532087.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1 alpha , c-Met, CA9 & GLUT1. HIF-1 alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), & strong staining intensity in squamous cell carcinoma (C). c-Met (D-F), CA9 (G, H) & GLUT1 (I) shows cell membranous staining. Representative c-Met expression in cervical samples shown with no staining in normal cervix (D), weak membranous staining intensity in squamous cell carcinoma (E) & strong intensity in squamous cell carcinoma (F). CA9 expression showing moderate intensity staining in carcinoma in situ (CIS) (G) & strong staining in adenocarcinoma (H). GLUT1 expression showing strong intensity in squamous cell carcinoma (I). Scale bar: 50 μm. Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241553723.jpg)
![Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Analysis of human IKK alpha in tumors of the three groups B (C-IKK alpha), E (Control), H (N-IKK alpha). Specific staining of human IKK alpha-using the NB100-56704 antibody. Scale bar: 70um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/8792/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunohistochemistry-Paraffin-NB100-56704-img0018.jpg "Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Analysis of human IKK alpha in tumors of the three groups B (C-IKK alpha), E (Control), H (N-IKK alpha). Specific staining of human IKK alpha-using the NB100-56704 antibody. Scale bar: 70um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/8792/text/) licensed under a CC-BY license.")
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Western blot showing the increased expression of IKK-alpha in transgenic mice. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/8792), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Western-Blot-NB100-56704-img0021.jpg)
![Flow Cytometry: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - An intracellular stain was performed on Hek293 cells with IKK alpha Antibody (14A231) NB100-56704 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Flow-Cytometry-NB100-56704-img0017.jpg)
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Total protein from various Human, Mouse and Rat cell lines were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-IKK-alpha in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Western-Blot-NB100-56704-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40X.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-56704-img0008.jpg)
![Immunohistochemistry: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - B-I. Immunohistochemistry showing the expression of the transgenic protein in N-IKKa and C-IKKa tumors. Staining with NB100-56704 antibody is shown. (B, C) Representative images showing the expression of transgenic IKKa in tumors and adjacent skin of N-IKKa/TgAC mice (B), and C-IKKa/TgAC animals (C). (D, E) Detail showing the nuclear (D) or cytoplasmic (E) localization of the transgenic IKKa in tumors. (F, G) Similar levels of expression of the transgenic IKKa in different N-IKKa tumors. By contrast variable levels of expression of the transgene are observed between different C-IKKa tumors (H, I). t: tumor; s: non-tumoral skin. Scale bar: (B, C) 100um; (D, E) 80 um; (F-I) 200 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/8792/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunohistochemistry-NB100-56704-img0022.jpg)
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Western-Blot-NB100-56704-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-IKK alpha Antibody (14A231) at 2 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-56704-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-IKK alpha Antibody (14A231) at 2 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-56704-img0015.jpg)
![Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of IKK alpha clone 14A231 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunohistochemistry-Paraffin-NB100-56704-img0016.jpg)
![Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Expression of the transgenic IKK alpha protein in skin of C-IKK alpha and N-IKK alpha mice. D-E. Expression of exogenous IKK alpha protein in back skin of 1-month-old mice. Immunostaining with the NB100-56704 anti-IKK alpha antibody is shown. Note the cytoplasmic expression of the transgene in the C-IKK alpha mice (D). By contrast, it is located in the nuclei of cells in the N-IKK alpha mice (E). In both types of transgenic mice the exogenous IKK alpha is expressed in basal keratinocytes (bk), in the outer root sheath of hair follicles (ORS) and in cells surrounding the sebaceous glands (sb). Scale bar: 60um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/8792/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Immunohistochemistry-Paraffin-NB100-56704-img0020.jpg)
![Simple Western: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Image shows a specific band for IKK alpha in 0.5 mg/mL of Daudi (left) and U937 (right) lysate. This exeriment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-14A231-BSA-Free-Simple-Western-NB100-56704-img0009.jpg)
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - NEMO-Ub binding is essential for TNF alpha signaling, but not for IL-1 beta signaling.(a) Schematic illustration of NEMO deletions or point mutations. (b,c) Effects of NEMO C-terminal deletion mutants on TNF alpha & IL-1 beta signaling. NEMO−/Y MEFs were reconstituted with mock, NEMO WT or different C-terminal deletion constructs & stimulated with TNF alpha (b) or IL-1 beta (c). Effects on NF-kappa B signaling were investigated by determining I kappa B alpha phosphorylation & degradation in Western Blots as well as NF-kappa B-DNA binding by EMSA. (d,e) Effects of NEMO D311N on TNF alpha & IL-1 beta signaling. NEMO−/Y MEFs were reconstituted with mock, NEMO WT or NEMO D311N point mutant & stimulated with TNF alpha (d) or IL-1 beta (e). Analysis on NF-kappa B signaling were performed as in (b,c). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26740240), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56704_mouse-monoclonal-ikk-alpha-antibody-14a231-bsa-free-310202415392529.jpg)
![Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Expression of the transgenic IKK alpha protein in skin of C-IKK alpha & N-IKK alpha miceA. Recombinant DNA constructs employed to generate both transgenic mice lines. For C-IKK alpha mice generation, the nuclear localization signal (NLS) was removed from the sequence of the human IKK alpha cDNA employed. In the construct used for generation of the N-IKK alpha mice an extra NLS signal was added. WT IKK alpha ; wild type IKK alpha . B. Western blot of total protein extracts showing IKK alpha expression in back skin of Control & C-and N-IKK alpha mice. Actin was used as a loading control. C. Representative example of the K5 staining in back skin section of Control mice. D-E. Expression of exogenous IKK alpha protein in back skin of 1-month-old mice. Immunostaining with the NB100-56704 anti-IKK alpha antibody is showed; similar results were obtained with the H00001147-M04 IKK alpha antibody (not shown). Note the cytoplasmic expression of the transgene in the C-IKK alpha mice (D). By contrast, it is located in the nuclei of cells in the N-IKK alpha mice (E). In both types of transgenic mice the exogenous IKK alpha is expressed in basal keratinocytes (bk), in the outer root sheath of hair follicles (ORS) & in cells surrounding the sebaceous glands (sb). F. Back skin section of Control mice. The NB100-56704 antibody used does not recognize the endogenous IKK alpha in immunohistochemical assays. G. Endogenous IKK alpha expression in control mice using the IKK alpha (sc-7182) antibody. Scale bar: (C) 70 μm; (D-G) 60 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27121058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56704_mouse-monoclonal-ikk-alpha-antibody-14a231-bsa-free-310202415523920.jpg)
![Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Expression of the transgenic IKK alpha protein in skin of C-IKK alpha & N-IKK alpha miceA. Recombinant DNA constructs employed to generate both transgenic mice lines. For C-IKK alpha mice generation, the nuclear localization signal (NLS) was removed from the sequence of the human IKK alpha cDNA employed. In the construct used for generation of the N-IKK alpha mice an extra NLS signal was added. WT IKK alpha ; wild type IKK alpha . B. Western blot of total protein extracts showing IKK alpha expression in back skin of Control & C-and N-IKK alpha mice. Actin was used as a loading control. C. Representative example of the K5 staining in back skin section of Control mice. D-E. Expression of exogenous IKK alpha protein in back skin of 1-month-old mice. Immunostaining with the NB100-56704 anti-IKK alpha antibody is showed; similar results were obtained with the H00001147-M04 IKK alpha antibody (not shown). Note the cytoplasmic expression of the transgene in the C-IKK alpha mice (D). By contrast, it is located in the nuclei of cells in the N-IKK alpha mice (E). In both types of transgenic mice the exogenous IKK alpha is expressed in basal keratinocytes (bk), in the outer root sheath of hair follicles (ORS) & in cells surrounding the sebaceous glands (sb). F. Back skin section of Control mice. The NB100-56704 antibody used does not recognize the endogenous IKK alpha in immunohistochemical assays. G. Endogenous IKK alpha expression in control mice using the IKK alpha (sc-7182) antibody. Scale bar: (C) 70 μm; (D-G) 60 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27121058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56704_mouse-monoclonal-ikk-alpha-antibody-14a231-bsa-free-3102024168399.jpg)
![Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0014.jpg "Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.")
![Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors were stained with anti-CD31, anti-IL-6, and anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal and tumor tissues. Bar represents 50?um. Zoom-in factor: 4 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03050-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1131-img0015.jpg)
![Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. a Human brain ECs were treated with glioma-CM for 24 h, & cell lysates were subjected to multiplex cytokine array analysis. Left, a representative blot. Right, quantified dot intensity of most significantly changed cytokines. b Human microvascular brain ECs were treated with glioma-CM that were harvested from different human glioma cells. Cell lysates were immunoblotted. c Human microvascular brain ECs & tumor-associated ECs isolated from different GBM patients were subjected to immunoblot analysis. d Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains & tumors were stained with anti-CD31, anti-IL-6, & anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal & tumor tissues. Bar represents 50 μm. Zoom-in factor: 4 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29422647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1131_rabbit-polyclonal-il-6-antibody-310202416154648.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (KP1) [NB100-683] - Representative images (40x) of infiltrated macrophages stained with anti-CD 68 in the gingiva. The black arrowheads indicate the infiltrated macrophages. The right graphs show the quantitative data of CD68-positive cells in HPF (magnification of 200x). Data are expressed as the mean +/- SEM of three slides. *p<0.05 compared with control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0102450) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Immunohistochemistry-NB100-683-img0009.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (KP1) [NB100-683] - Representative images (40x) of infiltrated macrophages stained with anti-CD 68 in the gingiva. The black arrowheads indicate the infiltrated macrophages. The right graphs show the quantitative data of CD68-positive cells in HPF (magnification of 200x). Data are expressed as the mean +/- SEM of three slides. *p<0.05 compared with control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0102450) licensed under a CC-BY license.")
![Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1:50 for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Flow-Cytometry-NB100-683-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (KP1) [NB100-683] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD68/SR-D1 Antibody [KP1] (NB100-683) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Immunocytochemistry-Immunofluorescence-NB100-683-img0012.jpg)
![Western Blot: CD68/SR-D1 Antibody (KP1) [NB100-683] - Total protein from mouse Raw cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD68 (NB100-683) in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Western-Blot-NB100-683-img0007.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on A431 cells with CD68/SR-D1 [KP1] Antibody NB100-683AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Flow-Cytometry-NB100-683-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (KP1) [NB100-683] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD68/SR-D1 Antibody [KP1] conjugated to Alexa Fluor 647 (NB100-683AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Immunocytochemistry-Immunofluorescence-NB100-683-img0011.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD68 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Immunohistochemistry-Paraffin-NB100-683-img0006.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68 Antibody [KP1] NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Flow-Cytometry-NB100-683-img0005.jpg)
![Dual RNAscope ISH-IHC: CD68/SR-D1 Antibody (KP1) [NB100-683] - NOS2 mRNA (red) and CD68 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-NOS2 and CD68/SR-D1 antibody (KP1) at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-Dual-RNAscope-ISH-IHC-NB100-683-img0010.jpg)
![Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Western-Blot-NB100-56509-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of IKK beta Antibody in HeLa cells. Antibody dilution 5 ug/ml. Image from verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunocytochemistry-Immunofluorescence-NB100-56509-img0016.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunohistochemistry-Paraffin-NB100-56509-img0014.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0012.jpg)
![Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Western-Blot-NB100-56509-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunocytochemistry-Immunofluorescence-NB100-56509-img0013.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control , red represents the IKKbeta antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0010.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0011.jpg)
![Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Simple-Western-NB100-56509-img0008.jpg)
![Western Blot: IKK beta Antibody (10AG2) - BSA Free [NB100-56509] - TRAM is required for the pro-inflammatory polarization of macrophages by SLD-LPS. (A,B) Flow cytometry analysis of Ly6C (A) or CD200R (B) expression in WT or TRAM−/− live-cell-gated macrophages with geometric MFI quantification on right. Pink = PBS, Blue = 100 pg/ml LPS. (C–E) Representative Western blots of IKK-beta (C), p-p65 (D), & total NF-kappa B p65 (E) expression in WT & TRAM−/− monocytes treated with either PBS or 100 pg/mL LPS for 5 days. Quantification is depicted on the right. (F) Ratio of p-p65/total p65 (p-p65 levels from (D) divided by corresponding total p65 levels). All data are representative of at least 3 separate experiments (n = 3) for (A,B) & n = 6 for (C–F). ANOVA with Tukey's multiple comparisons test (A,B); unpaired student t-test (C–F) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s, non-significant; MFI, Mean fluorescence intensity. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32765513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56509_mouse-monoclonal-ikk-beta-antibody-10ag2-31020241554520.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-310202416171426.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-31020241618941.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-310202416173525.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-31020241616378.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-3102024161609.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-310202416163753.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-562_mouse-monoclonal-cd31-pecam-1-antibody-jc-70a-310202416171483.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the FITC conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of rat brain cryosection with Mouse.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Frozen-NB100-64796-img0012.jpg "Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the FITC conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of rat brain cryosection with Mouse.")
![Flow Cytometry: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the Alexa Fluor (R) 488 conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of PBMC's (Monocyte gate at 1 x 10^6 cells/ml) with CD31 antibody (orange) stained at a dilution of 1:500. Shown with mIgG (AF488) isotype control (blue).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-TLD-3A12-Flow-Cytometry-NB100-64796-img0007.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis of CD31 in 4th day cutaneous wound section of diabetic rat using CD31/PECAM-1 Antibody (TLD-3A12). Image from verified customer review.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Paraffin-NB100-64796-img0005.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the FITC conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of rat spleen cryosection.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Frozen-NB100-64796-img0013.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification 200). n = 6, **P less than 0.01. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2019/9564869/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Paraffin-NB100-64796-img0014.jpg)
![Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Flow-Cytometry-NB100-77417-img0004.jpg "Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).")
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0013.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0003.jpg)
![Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Frozen-NB100-77417-img0008.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0002.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0009.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0012.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0011.jpg)
![Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - IHC and Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of PHD-2 on healed wounded skin and adjacent normal unwounded skin of wild type, heterozygous K14-Cre/homozygous floxed PHD-2, and heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice at 400x magnification. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-NB100-2219-img0015.jpg "Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - IHC and Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of PHD-2 on healed wounded skin and adjacent normal unwounded skin of wild type, heterozygous K14-Cre/homozygous floxed PHD-2, and heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice at 400x magnification. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: EGLN1/PHD2 Antibody [NB100-2219] - In vitro analysis of PHD-2 knockout and protein quantification.Western blot data for PHD-2 knockout in fibroblasts of heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice compared to wild type mice (*p<0.05). D') Western blot data for PHD-2 knockout in keratinocytes of heterozygous K14-Cre/homozygous floxed PHD-2 mice compared to wild type mice (*p<0.05). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Knockdown-Validated-NB100-2219-img0016.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of an FFPE mouse lung section using 1:200 dilution of EGLN1/PHD2 antibody. The staining was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The antibody generated a specific staining in the cytoplasm and nuclei of alveolar as well as bronchiolar epithelial cells. Cytoplasmic staining was observed in almost all cells while the nuclear positivity was seen in a subset of cells only.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-Paraffin-NB100-2219-img0014.jpg)
![Western Blot: EGLN1/PHD2 Antibody [NB100-2219] - Detection of EGLN1/PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Western-Blot-NB100-2219-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - EGLN1/PHD2 antibody at 1:500 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2219-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of EGLN1/PHD2 in ARPE-19 cells using anti-PHD2 antibody. ICC/IF image submitted by a veriifed customer review.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2219-img0009.jpg)
![Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Staining of renal tubular epithelium in mouse using NB100-2219 at 2.5 ug/mL.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-NB100-2219-img0010.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen cryosection.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunohistochemistry-Paraffin-NBP2-33337-img0009.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen cryosection.")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunohistochemistry-Paraffin-NBP2-33337-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Wehi-3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti CD68 (FA-11) [NBP2-33337] at a 1:100 dilution overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunocytochemistry-Immunofluorescence-NBP2-33337-img0012.jpg)
![Flow (Intracellular): CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw 246.7 cells with CD68/SR-D1 (FA-11) antibody NBP2-33337 (blue) and a matched isotype control MAB006 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-rat secondary antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Intracellular-NBP2-33337-img0011.jpg)
![Western Blot: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Expression on J774 cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Western-Blot-NBP2-33337-img0010.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw264.7 cells with CD68/SR-D1 Antibody (FA-11) NBP2-33337B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Cytometry-NBP2-33337-img0013.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Staining of permeabilised Mouse peritoneal Macrophages cells with Rat anti Mouse CD68 Antibody (Clone FA-11) visualised with F(ab')2 Goat Anti Rat IgG:FITC (Mouse Adsorbed).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Cytometry-NBP2-33337-img0007.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Analysis of CD11b/c in Raw 264.7 whole cell lysate.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Western-Blot-NB110-40766-img0002.jpg "Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Analysis of CD11b/c in Raw 264.7 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - CD11 antibody was tested in Raw264.7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-40766-img0006.jpg)
![Immunohistochemistry-Frozen: CD11b/c Antibody - BSA Free [NB110-40766] - 10 um Cryosection of murine acute brain slices stained with CD11b/c antibody (1:300 = 3.3 ug/ml) and Alexa Fluor 555 donkey anti rabbit IgG. Antibody shows specific staining, but the background is quite heavy. Image from verified customer review.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB110-40766-img0009.jpg)
![Flow Cytometry: CD11b/c Antibody - BSA Free [NB110-40766] - A surface stain was performed on RAW 264.7 with NB110-40766 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Flow-Cytometry-NB110-40766-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, phosphoinositide 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), and phosphorylated inhibitor of kB alpha (p-IkB alpha) in the spinal cord of the CCI-injured mice. The relative protein levels of (A) CD11b/c and (B) PI3K on the ipsilateral side of the spinal cord in the CCI-injured mice treated with oltipraz (OLT) or vehicle are represented. (C) Representative examples of blots for CD11b/c (160 kDa), PI3K (130 kDa), and GAPDH (37 kDa). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-c Antibody - BSA Free-Western Blot-NB110-40766-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - Phase contrast microscopy images of HFA and brain tumor cells and immunofluorescence images of cultured primary brain tumor cells, AA and HFA. HFA were derived from three different fetal brains. HFA from an 18-week- old fetus (passaged once) - GFAP (DAKO) merged with DAPI and CD68 (zoomed: 2.1x magnification); GFAP (green and red) was used as an astrocyte marker and CD68 (red) and CD11b (green) were used as markers for microglia. Nuclei indicated by DAPI (blue) in all images. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-40766-img0010.jpg)
![Immunohistochemistry: CD11b/c Antibody - BSA Free [NB110-40766] - Staining of human bone marrow, myeloid precursors.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunohistochemistry-NB110-40766-img0007.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, phosphoinositide 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), & phosphorylated inhibitor of kappa B alpha (p-IkB alpha ) in the spinal cord of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha on the ipsilateral side of the spinal cord in the CCI-injured mice treated with oltipraz (OLT) or vehicle are represented. The sham-operated mice (SHAM) treated with vehicle were used as controls. (C) Representative examples of blots for CD11b/c (160 kDa), PI3K (130 kDa), & GAPDH (37 kDa), & (F) for p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa) & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235533.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, & p-IkB alpha in the prefrontal cortex of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha in the prefrontal cortex of the CCI-injured mice treated with oltipraz (OLT) or vehicle. The sham-operated mice (SHAM) treated with vehicle were used as controls. Representative examples of blots for (C) CD11b/c (160 kDa), PI3K (130 kDa) & GAPDH (37 kDa), & for (F) p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa) & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 4-–5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235518.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, & p-IkB alpha in the hippocampus of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha in the hippocampus of the CCI-injured mice treated with oltipraz (OLT) or vehicle. The sham-operated mice (SHAM) treated with vehicle were used as controls. Representative examples of blots for (C) CD11b/c (160 kDa), PI3K (130 kDa), & GAPDH (37 kDa); & (F) for p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa), & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235538.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - Phase contrast microscopy images of HFA & brain tumor cells & immunofluorescence images of cultured primary brain tumor cells, AA & HFA.HFA were derived from three different foetal brains. (A) 18-week foetus (10× magnification); (B) 19-week foetus, first week in culture (10× magnification); (C) 19-week foetus, day 5 in culture (20× magnification); (D) Primary GBM after 2 trypsinisations (20x); (E) Recurrent GBM (20x); (F) Secondary GBM after 3 trypsinisations (10x); (G) OII 14 days in culture (10x); (H) AII 13 days in culture (10x); (I) Negative control for NGS merged with DAPI; (J) Negative control for secondary antibodies merged with DAPI; (K) GBM cells derived from one patient – GFAP (DAKO) merged with DAPI & CD68 (zoomed: 2.4× magnification); (L) primary brain tumor cells from one patient (male, 40 years old) (passaged three times) - GFAP (Novocastra) merged with DAPI; (K & L) GFAP (green) was used as a GBM marker & CD68 (red) was used as a marker for microglia. (M) AA from one Female, 61 years old; (passaged twice) - GFAP (Novocastra) merged with DAPI; (N) AA from one male 40 years old; (passaged once) - GFAP (Sigma) merged with DAPI & CD68 (Abcam); (M & N) GFAP (green) was used as an astrocyte marker & CD68 (red) & CD11b (green) were used as markers for microglia; (O) HFA from one 17-week- old foetus (passaged once) - GFAP (Novocastra) merged with DAPI & CD11b (Novus); (P) HFA from an 18-week- old foetus (passaged once) - GFAP (DAKO) merged with DAPI & CD68 (zoomed: 2.1× magnification); (O & P) GFAP (green & red) was used as an astrocyte marker & CD68 (red) & CD11b (green) were used as markers for microglia. Nuclei indicated by DAPI (blue) in all images. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235543.jpg)