CD31/PECAM-1 Antibody (TLD-3A12) [FITC]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, IHC
Clone
TLD-3A12
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC
Concentration
0.1 mg/ml

CD31/PECAM-1 Antibody (TLD-3A12) [FITC] Summary

Immunogen
Activated, Lewis rat derived microglial cells
Localization
Membrane; single pass type I membrane protein.
Specificity
Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
Application Notes
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC-P: The staining of endothelium may be enhanced on FFPE tissue by pre-treatment with 0.2M boric acid pH7.0, See Wilson et al. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using NB100-63703.

Reactivity Notes

Reacts with: Rhesus Monkey. Mouse reactivity reported in scientific literature (PMID: 28237322).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 1.0% BSA
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for CD31/PECAM-1 Antibody (TLD-3A12) [FITC]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NB100-63703)(7)


Showing Publications 1 - 7 of 7.
Publications using NB100-63703 Applications Species
Salehi-Had H, Roh MI, Giani A et al. Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization. PLoS One. 2011 Apr. [PMID: 21559527]
Nakao A, Huang CS, Stolz DB et al. Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 2011 Feb. [PMID: 20851811]
Willis CL, Meske DS, Davis TP et al. Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 2010 Nov. [PMID: 20700133]
Lochhead JJ, McCaffrey G, Quigley CE et al. Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 2010 Sep. [PMID: 20234382]
Thebault P, Lhermite N, Tilly G et al. The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 2009 Sep. [PMID: 19667084]
Stevenson KS, McGlynn L, Hodge M et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009 Dec. [PMID: 19326970]
Kielian T, Hickey WF. Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 2000 Aug. [PMID: 10934167]

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-63701

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Bioinformatics Tool for CD31/PECAM-1 Antibody (NB100-63703)

Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NB100-63703). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1
Entrez