IL-6 Antibody

Images

 
Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 ...read more
Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors ...read more
Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

IL-6 Antibody Summary

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli. (Uniprot: P05231)
Localization
Secreted
Specificity
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts).
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
IL6
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:400-1:800
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400 - 1:800
  • Immunoprecipitation 1:400-1:800
  • Knockdown Validated
  • Western Blot 1:500-1:2000
Application Notes
IL-6 antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, and immunoblot. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. For immunohistochemistry either paraffin fix or cryofix tissue
Theoretical MW
23.72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
IL-6 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 1
using
NB600-1131 in the following applications:

Publications
Read Publications using
NB600-1131 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33651836).

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Purity
Unpurified

Notes

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for IL-6 Antibody

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

The antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule (21-28 kDa). The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1131
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD

Publications for IL-6 Antibody (NB600-1131)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Monkey.

We have publications tested in 7 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
ELISA
(1)
ICC/IF
(4)
IHC
(11)
IHC-Fr
(1)
IHC-P
(10)
KD
(1)
WB
(11)
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Filter By Species
Human
(8)
Mouse
(20)
Rat
(6)
Monkey
(1)
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB600-1131 Applications Species
Chow SKH, Cui C, Cheng KYK Et al. Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway International journal of molecular sciences Aug 13 2021 [PMID: 34445423] (IHC, Mouse) IHC Mouse
Koga Y, Sotokawauchi A, Higashimoto Y Et al. DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice Oxidative medicine and cellular longevity Aug 7 2021 [PMID: 34413930]
Singh J, Thachil T, Eapen M Et Al. Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound-guided needle biopsy specimens of prostate adenocarcinoma Molecular and Clinical Oncology Jul 16 2021 [PMID: 34405051] (IHC-P) IHC-P
Li Q, Qin L, Li J IL-6 signaling pathway contributes to exercise pressor reflex in rats with femoral artery occlusion in association with Kv4 activity in muscle afferent nerves Physiological reports Jul 1 2021 [PMID: 34231965] (IHC) IHC
Betsou A, Lambropoulou M, Georgakopoulou AE et al. The hepatoprotective effect of silibinin after hepatic ischemia/reperfusion in a rat model is confirmed by immunohistochemistry and qRT-PCR The Journal of pharmacy and pharmacology Apr 13 2021 [PMID: 33847359]
Subburaman M, Edderkaoui B Evaluation of CCL21 role in post-knee injury inflammation and early cartilage degeneration PloS one Mar 2 2021 [PMID: 33651836] (IHC-P, Rat) IHC-P Rat
Kanno SI, Hara A The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin-induced cardiotoxicity Molecular medicine reports Feb 1 2021 [PMID: 33300057] (ICC/IF, Rat) ICC/IF Rat
Shao H, Li Y, Pastar I et al. Notch1 signaling determines the plasticity and function of fibroblasts in diabetic wounds Life Sci Alliance Dec 1 2020 [PMID: 33109684]
Balakrishnan I, Danis E, Pierce A et al. Involvement of HO-1 and Autophagy in the Protective Effect of Magnolol in Hepatic Steatosis-Induced NLRP3 Inflammasome Activation In Vivo and In Vitro Cell Rep 2020 Sep 27 [PMID: 33086074] (WB, IHC, Human)

Details:
Citation using the IHC-Prediluted format of this antibody
WB, IHC Human
Balakrishnan I, Danis E, Pierce A et al. Involvement of HO-1 and Autophagy in the Protective Effect of Magnolol in Hepatic Steatosis-Induced NLRP3 Inflammasome Activation In Vivo and In Vitro Cell Rep 2020 Sep 27 [PMID: 33086074] (WB, IHC, Human)

Details:
Citation using the IHC-Prediluted format of this antibody
WB, IHC Human
Show All 44 Publications.

Review for IL-6 Antibody (NB600-1131) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-1131:
Filter by Applications
Simple Western
(1)
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Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western IL-6 NB600-1131
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1
reviewed by:
Lenka Uherková
Simple Western Rat 02/12/2020
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Summary

ApplicationSimple Western
Sample Testedskin wound - healthy
SpeciesRat
Lot38801

Comments

CommentsLow Rating Note: Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-6 Antibody (NB600-1131). (Showing 1 - 2 of 2 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
  2. I had a quick question regarding NB600-1131SS IL-6 antibody. Not sure if you guys would be able to answer but how low do you think I could go on protein amount loaded to pick up a band? I see on the data sheet you guys have loaded 50ug and I cant load that much based on the nature of my samples.
    • We usually recommend anywhere from 10-50 ug/lane of protein. I wouldn't recommend using much lower than 10 ug of sample.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP1-42761
HRP NBP1-42762

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Blogs on IL-6. Showing 1-10 of 12 blog posts - Show all blog posts.


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Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.


  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Lenka Uherková
02/12/2020
Application: Simple Western
Species: Rat

Bioinformatics

Gene Symbol IL6
Entrez
Uniprot