CD68/SR-D1 Antibody (KP1)

Images

 
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a formalin fixed paraffin embedded human spleen tissue section using 1:10 dilution of CD68/SR-D1 antibody (clone KP1) with HRP-DAB ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
KP1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD68/SR-D1 Antibody (KP1) Summary

Immunogen
Subcellular fraction of human alveolar macrophages.
Localization
Cell membrane
Marker
Macrophage Marker
Specificity
This is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD68
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:200
  • Immunohistochemistry-Frozen 1:10-1:200
  • Immunohistochemistry-Paraffin 1:10-1:200
  • Immunoprecipitation
Application Notes
This CD68 Antibody (KP1) is useful for Immunohistochemistry on frozen fixed and paraffin-emdedded sections, Immunoprecipitation, Western blot and ICC/IF. IHC: For an ABC system, dilute 1:20 and incubate for 30-60 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Flow Cytometry reported in scientific literature (PMID 18405323)
Control
CD68/SR-D1 Lysate (NBL1-08962)
CD68/SR-D1 Lysate (NBP2-64803)
Publications
Read Publications using
NB100-683 in the following applications:

  • 2 publications
  • 4 publications
  • IHC
    14 publications
  • 6 publications
  • WB
    2 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25058444)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tissue culture supernatant
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD68/SR-D1 Antibody (KP1)

  • CD68 antigenmacrophage antigen CD68
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

CD68, also known as GP110, LAMP4, and SCARD1, encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissues. CD68 is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favors binding to lectins through its heavily glycosylated extracellular domain. Furthermore, CD68 has also been found to be a member of the scavenger receptor family, whose main purpose is to clear cellular debris and regulate phagocytosis and macrophage recruitment. Recent studies have investigated the role of tumor related macrophages using CD68 as a probe (PMID: 22931216), as well as their involvement in the progression of other cancers (PMID: 21113139).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (NB100-683)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(4)
IHC
(14)
IHC-P
(6)
WB
(2)
All Applications
Filter By Species
Human
(16)
Mouse
(7)
Rat
(2)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-683 Applications Species
Ao JY, Zhu XD, Chai ZT et al. Colony-stimulating factor 1 receptor blockade inhibits tumor growth by altering the polarization of tumor-associated macrophages in hepatocellular carcinoma. Mol. Cancer Ther. 2017 Jun 01 [PMID: 28572167] (IHC-P, Mouse) IHC-P Mouse
Ayari H, Legedz L, Lantelme P et al. Auto-amplification of cortisol actions in human carotid atheroma is linked to arterial remodeling and stroke Fundamental & Clinical Pharmacology 2012 Jul 20 (IHC, Human) IHC Human
Liu X, Guo X, Li J et al. Interferon-gamma Aggravated L-Arginine-Induced Acute Pancreatitis in Sprague-Dawley Rats and Its Possible Mechanism: Trypsinogen Activation and Autophagy Up-regulation. Pancreas. Apr 4 2017 [PMID: 28375949] (Rat) Rat
Feng X, Weng D, Zhou F et al. Activation of PPARgamma by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization. EBioMedicine Jun 15 2016 [PMID: 27374313] (IHC, Mouse) IHC Mouse
Miao H, Ou J, Peng Y et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun. May 18 2016 [PMID: 27189574] (IHC-P, Human) IHC-P Human
Koh MY, Gagea M, Sargis T et al. A new HIF-1alpha/RANTES driven pathway to hepatocellular carcinoma mediated by germline haploinsufficiency of SART1/HAF. Hepatology. 2016 Jan 22 [PMID: 26799785]
Pandurangan AK, Ismail S, Saadatdoust Z. Allicin Alleviates Dextran Sodium Sulfate-(DSS-) Induced Ulcerative Colitis in BALB/c Mice. Oxidative Medicine and Cellular Longevity. 2015 [PMID: 26075036] (IHC-P, Mouse) IHC-P Mouse
Lin FY, Hsiao FP, Huang CY et al. Porphyromonas gingivalis GroEL Induces Osteoclastogenesis of Periodontal Ligament Cells and Enhances Alveolar Bone Resorption in Rats. PLoS ONE 2014 Jul 25 [PMID: 25058444] (IHC-P, Rat)

Details:
CD68 antibody used in IHC-P for detecting the inflammatory infiltration of the gingival connective tissue of Rats inoculated or not with Porphyromonas gingivalis GroEL (inoculated into rat gingiva). Figure 5D - GroEL induced macrophages infiltration in rat gingival as marked by CD68 expression.
IHC-P Rat
Joshi-Barr S, Bett C, Chiang WC et al. De novo prion aggregates trigger autophagy in skeletal muscle. J Virol 2013 Dec 4 [PMID: 24307586] (IHC-P, Mouse) IHC-P Mouse
Herwig MC, Bergstrom C, Wells JR et al. M2/M1 ratio of tumor associated macrophages and PPAR-gamma expression in uveal melanomas with class 1 and class 2 molecular profiles Exp Eye Res 2012 Nov 30 [PMID: 23206928] (IHC, Human) IHC Human
Show All 34 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD68/SR-D1 Antibody (NB100-683) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD68/SR-D1.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

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Bioinformatics

Gene Symbol CD68
Entrez
Uniprot