CD68/SR-D1 Antibody (KP1)

Images

 
Western Blot: CD68/SR-D1 Antibody (KP1) - Unpurified [NB100-683] - Total protein from human spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) - Unpurified [NB100-683] - CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a FFPE human spleen tissue section using 1:10 dilution of CD68/SR-D1 antibody ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
KP1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD68/SR-D1 Antibody (KP1) Summary

Immunogen
Subcellular fraction of human alveolar macrophages.
Localization
Cell membrane
Marker
Macrophage Marker
Specificity
This is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD68
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1-2 ug/million cells/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:200
  • Immunohistochemistry-Frozen 1:10-1:200
  • Immunohistochemistry-Paraffin 1:10-1:200
  • Immunoprecipitation
Application Notes
IHC: For an ABC system, dilute 1:20 and incubate for 30-60 minutes at RT. IHC-P tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Flow Cytometry reported in scientific literature (PMID: 18405323).
Control
CD68/SR-D1 Overexpression Lysate
CD68/SR-D1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-683 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25058444)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tissue culture supernatant
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD68/SR-D1 Antibody (KP1)

  • CD68 antigenmacrophage antigen CD68
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

CD68, also known as GP110, LAMP4, and SCARD1, encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissues. CD68 is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favors binding to lectins through its heavily glycosylated extracellular domain. Furthermore, CD68 has also been found to be a member of the scavenger receptor family, whose main purpose is to clear cellular debris and regulate phagocytosis and macrophage recruitment. Recent studies have investigated the role of tumor related macrophages using CD68 as a probe (PMID: 22931216), as well as their involvement in the progression of other cancers (PMID: 21113139).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (NB100-683)(42)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(7)
IHC
(16)
IHC-Fr
(1)
IHC-P
(8)
WB
(2)
All Applications
Filter By Species
Human
(23)
Mouse
(8)
Rat
(2)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB100-683 Applications Species
Wu Y, Qin YH, Liu Y et al. Cardiac troponin I autoantibody induces myocardial dysfunction by PTEN signaling activation EBioMedicine Aug 29 2019 [PMID: 31474552] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Liu W, Kampfe Nordstrom C, Danckwardt-Lilliestrom N, Rask-Andersen H, Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study Front Neurol Jul 10 2019 [PMID: 31354608] (IHC-Fr, Human) IHC-Fr Human
Ding Jinli, Yin Tailang, Yan Nana et al. FasL on decidual macrophages mediates trophoblast apoptosis: A potential cause of recurrent miscarriage. International Journal of Molecular Medicine 2019 Mar 21 [PMID: 30942389] (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human
Triulzi T, Forte L, Regondi V et al. HER2 signaling regulates the tumor immune microenvironment and trastuzumab efficacy OncoImmunology Sep 21 2018 [PMID: 30546951] (IHC, Human) IHC Human
Triulzi T, Regondi V, De Cecco L et al. Early immune modulation by single-agent trastuzumab as a marker of trastuzumab benefit. Br. J. Cancer. Nov 27 2018 [PMID: 30478407] (ICC/IF, Human) ICC/IF Human
van Rooijen JM, Qiu SQ, Timmer-Bosscha H et al. Androgen receptor expression inversely correlates with immune cell infiltration in human epidermal growth factor receptor 2-positive breast cancer. Eur. J. Cancer Sep 9 2018 [PMID: 30208359] (ICC/IF, Human) ICC/IF Human
Li, Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis Aug 7 2018 [PMID: 30082761] (IHC, Human) IHC Human
Ao JY, Zhu XD, Chai ZT et al. Colony-stimulating factor 1 receptor blockade inhibits tumor growth by altering the polarization of tumor-associated macrophages in hepatocellular carcinoma. Mol. Cancer Ther. 2017 Jun 01 [PMID: 28572167] (IHC-P, Mouse) IHC-P Mouse
Liu X, Guo X, Li J et al. Interferon-gamma Aggravated L-Arginine-Induced Acute Pancreatitis in Sprague-Dawley Rats and Its Possible Mechanism: Trypsinogen Activation and Autophagy Up-regulation. Pancreas. Apr 4 2017 [PMID: 28375949] (Rat) Rat
Feng X, Weng D, Zhou F et al. Activation of PPARgamma by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization. EBioMedicine Jun 15 2016 [PMID: 27374313] (IHC, Mouse) IHC Mouse
Show All 42 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD68/SR-D1 Antibody (NB100-683) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-683AF350
Alexa Fluor 405 NB100-683AF405
Alexa Fluor 488 NB100-683AF488
Alexa Fluor 532 NB100-683AF532
Alexa Fluor 594 NB100-683AF594
Alexa Fluor 647 NB100-683AF647
Alexa Fluor 700 NB100-683AF700
Alexa Fluor 750 NB100-683AF750
Allophycocyanin NB100-683APC
Biotin NB100-683B
DyLight 350 NB100-683UV
DyLight 405 NB100-683V
DyLight 488 NB100-683G
DyLight 550 NB100-683R
DyLight 594 NB100-683DL594
DyLight 650 NB100-683C
DyLight 680 NB100-683FR
DyLight 755 NB100-683IR
FITC NB100-683F
HRP NB100-683H
Janelia Fluor 549 NB100-683JF549
Janelia Fluor 646 NB100-683JF646
PE NB100-683PE
PerCP NB100-683PCP

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Blogs on CD68/SR-D1.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

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Bioinformatics

Gene Symbol CD68
Entrez
Uniprot