CD68/SR-D1 Antibody (KP1)

Images

 
Western Blot: CD68/SR-D1 Antibody (KP1) [NB100-683] - Total protein from mouse Raw cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD68 antibody. The staining was developed using HRP labeled anti-rabbit ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68 Antibody [KP1] NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
KP1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD68/SR-D1 Antibody (KP1) Summary

Immunogen
Subcellular fraction of human alveolar macrophages.
Localization
Cell membrane
Marker
Macrophage Marker
Specificity
This is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD68
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation
Application Notes
IHC: For an ABC system, dilute 1:20 and incubate for 30-60 minutes at RT. IHC-P tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Flow Cytometry reported in scientific literature (PMID: 18405323). Use in Electron Microscopy reported in scientific literature (PMID: 8962141).
Control
CD68/SR-D1 Overexpression Lysate
CD68/SR-D1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-683 in the following applications:

Publications
Read Publications using
NB100-683 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25058444)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD68/SR-D1 Antibody (KP1)

  • CD_antigen: CD68
  • CD68 antigen
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • LAMP4
  • macrophage antigen CD68
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

CD68, also known as GP110, LAMP4, and SCARD1, encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissues. CD68 is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favors binding to lectins through its heavily glycosylated extracellular domain. Furthermore, CD68 has also been found to be a member of the scavenger receptor family, whose main purpose is to clear cellular debris and regulate phagocytosis and macrophage recruitment. Recent studies have investigated the role of tumor related macrophages using CD68 as a probe (PMID: 22931216), as well as their involvement in the progression of other cancers (PMID: 21113139).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (NB100-683)(49)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(9)
IF
(1)
IHC
(19)
IHC-Fr
(1)
IHC-P
(9)
WB
(3)
All Applications
Filter By Species
Human
(25)
Mouse
(10)
Rat
(5)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using NB100-683 Applications Species
McCloskey CW, Cook DP, Kelly BS et al. Metformin abrogates age-associated ovarian fibrosis Clin. Cancer Res. 2019 Oct 09 [PMID: 31597663] (IHC-P, IHC, Human, Mouse) IHC-P, IHC Human, Mouse
Liu X, Chen J, Zhang B et al. KDM3A inhibition modulates macrophage polarization to aggravate post-MI injuries and accelerates adverse ventricular remodeling via an IRF4 signaling pathway Cell. Signal. Sep 9 2019 [PMID: 31513837] (IF, WB, Rat) IF, WB Rat
Wu Y, Qin YH, Liu Y et al. Cardiac troponin I autoantibody induces myocardial dysfunction by PTEN signaling activation EBioMedicine Aug 29 2019 [PMID: 31474552] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Liu W, Kampfe Nordstrom C, Danckwardt-Lilliestrom N, Rask-Andersen H, Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study Front Neurol Jul 10 2019 [PMID: 31354608] (IHC-Fr, Human) IHC-Fr Human
Ding Jinli, Yin Tailang, Yan Nana et al. FasL on decidual macrophages mediates trophoblast apoptosis: A potential cause of recurrent miscarriage. International Journal of Molecular Medicine 2019 Mar 21 [PMID: 30942389] (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human
van Rooijen JM, Qiu SQ, Timmer-Bosscha H et al. Androgen receptor expression inversely correlates with immune cell infiltration in human epidermal growth factor receptor 2-positive breast cancer. Eur. J. Cancer Sep 9 2018 [PMID: 30208359] (ICC/IF, Human) ICC/IF Human
Ao JY, Zhu XD, Chai ZT et al. Colony-stimulating factor 1 receptor blockade inhibits tumor growth by altering the polarization of tumor-associated macrophages in hepatocellular carcinoma. Mol. Cancer Ther. 2017 Jun 01 [PMID: 28572167] (IHC-P, Mouse) IHC-P Mouse
Liu X, Guo X, Li J et al. Interferon-gamma Aggravated L-Arginine-Induced Acute Pancreatitis in Sprague-Dawley Rats and Its Possible Mechanism: Trypsinogen Activation and Autophagy Up-regulation. Pancreas. Apr 4 2017 [PMID: 28375949] (Rat) Rat
Miao H, Ou J, Peng Y et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun. May 18 2016 [PMID: 27189574] (IHC-P, Human) IHC-P Human
Triulzi T, Forte L, Regondi V et al. HER2 signaling regulates the tumor immune microenvironment and trastuzumab efficacy PLoS ONE Sep 28 2018
Show All 49 Publications.

Review for CD68/SR-D1 Antibody (NB100-683) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-683:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD68/SR-D1 NB100-683
Enlarge
reviewed by:
KARTHIKEYAN THIRUGNANAM
IHC-P Mouse 11/22/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse heart
SpeciesMouse
Lot263360

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD68/SR-D1 Antibody (NB100-683) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-683AF350
Alexa Fluor 405 NB100-683AF405
Alexa Fluor 488 NB100-683AF488
Alexa Fluor 532 NB100-683AF532
Alexa Fluor 594 NB100-683AF594
Alexa Fluor 647 NB100-683AF647
Alexa Fluor 700 NB100-683AF700
Alexa Fluor 750 NB100-683AF750
Allophycocyanin NB100-683APC
Biotin NB100-683B
DyLight 350 NB100-683UV
DyLight 405 NB100-683V
DyLight 488 NB100-683G
DyLight 550 NB100-683R
DyLight 594 NB100-683DL594
DyLight 650 NB100-683C
DyLight 680 NB100-683FR
DyLight 755 NB100-683IR
FITC NB100-683F
HRP NB100-683H
Janelia Fluor 549 NB100-683JF549
Janelia Fluor 646 NB100-683JF646
PE NB100-683PE
PerCP NB100-683PCP

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Bioinformatics Tool for CD68/SR-D1 Antibody (NB100-683)

Discover related pathways, diseases and genes to CD68/SR-D1 Antibody (NB100-683). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for CD68/SR-D1 Antibody (NB100-683)

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Blogs on CD68/SR-D1.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

KARTHIKEYAN THIRUGNANAM
11/22/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CD68
Entrez
Uniprot