CD68/SR-D1 Antibody (KP1) - BSA Free

Images

 
Immunohistochemistry: CD68/SR-D1 Antibody (KP1) [NB100-683] - Representative images (40x) of infiltrated macrophages stained with anti-CD 68 in the gingiva. The black arrowheads indicate the infiltrated macrophages. The ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (KP1) [NB100-683] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: CD68/SR-D1 Antibody (KP1) [NB100-683] - Total protein from mouse Raw cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on A431 cells with CD68/SR-D1 [KP1] Antibody NB100-683AF647 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (KP1) [NB100-683] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD68 antibody. The staining was developed using HRP labeled anti-rabbit ...read more
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68 Antibody [KP1] NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Dual RNAscope ISH-IHC: CD68/SR-D1 Antibody (KP1) [NB100-683] - NOS2 mRNA (red) and CD68 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MS, Dual ISH-IHC
Clone
KP1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD68/SR-D1 Antibody (KP1) - BSA Free Summary

Additional Information
Clone KP1 was used by HLDA to establish CD designation.
Immunogen
This CD68/SR-D1 Antibody (KP1) was developed against subcellular fraction of human alveolar macrophages.
Localization
Cell membrane
Marker
Macrophage Marker
Specificity
This CD68/SR-D1 Antibody (KP1) is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD68
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Electron Microscopy reported in scientific literature (PMID 8962141)
  • Flow Cytometry reported in scientific literature (PMID 18405323)
  • Immunocytochemistry/ Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation
  • Western Blot 2 ug/ml
Application Notes
IHC: For an ABC system, dilute 1:20 and incubate for 30-60 minutes at RT. IHC-P tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Control
CD68/SR-D1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-683 in the following applications:

Publications
Read Publications using
NB100-683 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25058444)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD68/SR-D1 Antibody (KP1) - BSA Free

  • CD_antigen: CD68
  • CD68 antigen
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • LAMP4
  • macrophage antigen CD68
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (NB100-683)(64)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: FLOW, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-P, In Vivo, WB.


Filter By Application
FLOW
(1)
ICC/IF
(11)
IF
(1)
IF/IHC
(20)
IHC
(2)
IHC-Fr
(5)
IHC-P
(15)
In Vivo
(1)
WB
(3)
All Applications
Filter By Species
Human
(30)
Mouse
(14)
Rat
(5)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using NB100-683 Applications Species
Pan Y, Hu Q, Yang Y et al. Characterization of pain-related behaviors and gene expression profiling of peripheral sensory ganglia in a mouse model of acute ankle sprain Frontiers in Behavioral Neuroscience 2023-05-25 [PMID: 37304762] (IHC-Fr, Mouse) IHC-Fr Mouse
Lim CS, Veltri B, Kashon M et al. Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 macrophages in�vitro Nanotoxicology 2023-04-28 [PMID: 37115655]
Zhai M, Gong S, Luan P et al. Extracellular traps from activated vascular smooth muscle cells drive the progression of atherosclerosis Nature communications 2022-12-06 [PMID: 36473863] (WB, IHC-Fr, IHC-P, Mouse, Human) WB, IHC-Fr, IHC-P Mouse, Human
Kater MSJ, Huffels CFM, Oshima T et al. Prevention of microgliosis halts early memory loss in a mouse model of Alzheimer's disease Brain, behavior, and immunity 2022-10-18 [PMID: 36270437] (IHC-Fr, Mouse)

Details:
Dilution used for IHC 1:100
IHC-Fr Mouse
Real F, Zhu A, Huang B et al. S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature communications 2022-10-11 [PMID: 36220814] (IHC-P, Human) IHC-P Human
Zhang J, Yin Z, Yu L et al. Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice Oxidative Medicine and Cellular Longevity 2022-09-01 [PMID: 36092156] (IHC-Fr, ICC/IF, IHC-P) IHC-Fr, ICC/IF, IHC-P
Stock AT, Parsons S, Sharma VJ et al. Intimal macrophages develop from circulating monocytes during vasculitis Clinical & translational immunology 2022-08-13 [PMID: 35991774] (IHC-P, Human) IHC-P Human
Zhang T, Zhang Y, Yang Z et al. Echinococcus multilocularis protoscoleces enhance glycolysis to promote M2 Macrophages through PI3K/Akt/mTOR Signaling Pathway Pathogens and global health 2023-06-01 [PMID: 35876088] (IHC-P, Human) IHC-P Human
Rivellese F, Surace AEA, Goldmann K Et al. Rituximab versus tocilizumab in rheumatoid arthritis: synovial biopsy-based biomarker analysis of the phase 4 R4RA randomized trial Nat Med 2022-05-19 [PMID: 35589854] (IHC-P, Human)

Details:
Citation using the Alexa Fluor 532 version of this antibody.
IHC-P Human
Zhang, S;Zhou, Y;Xian, H;Shi, Y;Liu, Y;Li, Z;Huang, Y; Nerve regeneration in rat peripheral nerve allografts: An assessment of the role of endogenous neurotrophic factors in nerve cryopreservation and regeneration The European journal of neuroscience [PMID: 35332602]
Show All 64 Publications.

Review for CD68/SR-D1 Antibody (NB100-683) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-683:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD68/SR-D1 NB100-683
Enlarge
5
reviewed by:
KARTHIKEYAN THIRUGNANAM
IHC-P Mouse 11/22/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse heart
SpeciesMouse
Lot263360

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD68/SR-D1 Antibody (NB100-683) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD68/SR-D1.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD ...  Read full blog post.

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KARTHIKEYAN THIRUGNANAM
11/22/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CD68
Entrez
Uniprot